21 results about "Extracellular polymeric substance" patented technology

This invention relates to the field of catalytic degradation technology for organic pollutants, and particularly to an iron-modified biochar immobilized catalase, its preparation method, and its application. The preparation method of the iron-modified biochar immobilized catalase includes the following steps: mixing iron-modified biochar, catalase solution, and an extracellular polymeric substance; performing an immobilization and cross-linking reaction; and separating the solid and liquid phases to obtain the iron-modified biochar immobilized catalase; the extracellular polymeric substance is secreted by *Goldenella*. This invention uses an extracellular polymeric substance instead of traditional glutaraldehyde as a cross-linking agent, avoiding the hazards associated with glutaraldehyde. Simultaneously, the prepared iron-modified biochar immobilized catalase exhibits higher enzyme activity and superior pollutant degradation performance.

This invention discloses a method for extracting extracellular polymers from benthic microalgae culture medium, as well as the products and applications thereof, comprising the following steps: adding formamide to the benthic microalgae culture medium, adding Na... + The extracellular polymeric substance (ECPL) is obtained by stirring, centrifuging, heating and concentrating, freeze-drying, dissolving in water, adding anhydrous ethanol, precipitating, dissolving again in water, dialyzing, and freeze-drying. This invention improves upon existing ethanol precipitation processes by employing an extraction process of "heating and concentrating benthic microalgae culture medium—freeze-drying to obtain a crude extract—redissolving the crude extract in water—precipitating with anhydrous ethanol—dissolving the precipitate—dialysis—freeze-drying to obtain extracellular polymeric substances." Compared to traditional extraction processes, this method allows for "controllable" ethanol dosage by dissolving a large amount of crude extract in a small amount of water to obtain a solution rich in extracellular polymeric substances. Furthermore, it significantly reduces the amount of anhydrous ethanol used when extracting large quantities of extracellular polymeric substances, greatly lowering experimental costs.

This invention relates to a method for the simultaneous capture of carbon and phosphorus in wastewater. It addresses the problems of high aeration energy consumption, long hydraulic retention time, low phosphorus removal rate, and high carbon, ammonia, and phosphorus levels in existing wastewater treatment processes. The method utilizes the aerobic phosphorus absorption characteristics of microorganisms, employing tightly bound EPS (extracellular polymeric substances) to rapidly store and release phosphorus, capturing it in the sludge phase. This simultaneously and rapidly reduces COD and total phosphorus in the wastewater to low levels, achieving a COD removal rate of over 70%. Furthermore, the proportion of carbon captured and converted into the sludge phase reaches 30%–37%, while the proportion of organic matter oxidized to CO2 is only 22%–31%, resulting in an effective phosphorus capture rate of 70%–75%, achieving highly efficient capture and removal of both phosphorus and organic matter. Simultaneously, for effluent treatment with irrigation potential, the method achieves phosphorus capture ratio control based on efficient carbon capture, thereby obtaining effluent with low COD concentration and dynamically adjustable nitrogen-to-phosphorus ratio to meet target irrigation needs.

This invention relates to the field of nanomaterials technology, and discloses a method for preparing and applying gold nanoparticles synthesized using microbial extracellular polymers (EPS). The method uses methylbazin exopolymers (EPS) as a green reducing agent and stabilizer, reacting them with chloroauric acid in an aqueous phase. By adjusting the EPS concentration, pH, and temperature, gold nanoparticles (EPS-Au NPs) can be synthesized in a controlled manner. The resulting nanoparticles exhibit high crystallinity and good dispersibility, and demonstrate ultra-high activity in catalyzing the reduction of 4-nitrophenol, with a TOF value reaching 485.53 h⁻¹. ‑1 This invention is superior to many traditional catalysts and has good cycle stability. The process of this invention is green and controllable, and the resulting catalyst has promising applications in wastewater treatment and other fields.

This invention provides a composite microbial agent for remediating cadmium pollution mediated by polyethylene microplastics, its preparation method, and its application. The composite microbial agent comprises directed-acclimated Bacillus subtilis and Rhodopseudomonas palustris. Applied to tea garden soil contaminated with cadmium mediated by polyethylene microplastics, the directed-acclimated Bacillus subtilis and Rhodopseudomonas palustris efficiently secrete extracellular polymeric substances (EPS) in the presence of PE, achieving physical encapsulation of PE particles and chemical complexation with Cd. On the one hand, it encapsulates PE microplastics, blocking their Cd adsorption sites; on the other hand, it complexes and passivates available Cd, thus remediating cadmium pollution mediated by polyethylene microplastics. Under a concentration gradient, the optimal concentration ( ) treatment can reduce soil DTPA-Cd content by 27.27%, TCLP-Cd content by 46.88%, Cd content in tea seedling leaves by 36.67%, and Cd translocation coefficient (TF) by 53.32%, simultaneously reducing soil environmental risk and cadmium accumulation in plants, achieving systematic control from soil to plants.

The present application belongs to the field of wastewater biological treatment and microbial electrochemistry technology, and particularly relates to a method for optimizing the ratio of living cells to dead cells and metabolic activity in the electrode surface biofilm by regulating the applied voltage in an inhibitory environment containing toxic substance 5-hydroxymethylfurfural. The present application significantly improves the ratio of living cells in the biofilm and promotes the secretion of functional extracellular polymeric substance by applying a direct current voltage of 0.8-1.4V in an anaerobic electro-fermentation system, thereby enhancing the system's resistance to toxicity. Experiments show that under a voltage of 1.2V, the ratio of living cells in the biofilm can reach more than 85%, the fluorescence intensity of protein-like and humic acid-like substances in the extracellular polymeric substance is the strongest, and the methane production is the highest and the system startup time is the shortest. The present application optimizes the biofilm structure and metabolic function by voltage regulation, thereby providing a systematic solution for the enhanced treatment and resource recovery of inhibitory wastewater.

This invention provides a confined conductive composite membrane, its preparation method, and an electrochemical method for in-situ hydrolysis of extracellular polymeric substances (EPS) based thereon, relating to the field of wastewater treatment technology. The confined conductive composite membrane comprises an ultrafiltration membrane carrier and a conductive layer formed on its surface. The conductive layer is composed of carbon nanotubes, with at least one of nano-Ni, Co, Fe, and Mo catalysts selectively loaded onto the inner wall of the hollow tubes or between the two-dimensional layers. This structure effectively prevents catalyst aggregation and loss through the confinement effect, improving the catalyst's dispersibility and stability. Simultaneously, the continuous conductive network formed by the carbon nanotubes possesses excellent electron transport capabilities, enabling rapid electron transfer to the catalytically active sites. When the composite membrane of this application operates as a cathode, the loaded nano-metal catalyst significantly reduces the overpotential of the hydrogen evolution reaction, inducing the in-situ construction of an alkaline microenvironment at low voltage, promoting the hydrolytic degradation of EPS attached to the membrane surface.

The application discloses a fluid solidified soil for reinforcing coastal soft soil and a preparation method thereof, and belongs to the technical field of soft soil treatment, and comprises coastal soft soil, cement, slag, fly ash, a composite functional microbial agent, a composite functional stabilizer, an auxiliary microbial agent, a biological carrier and water; the composite functional microbial agent is one or more of sulfate-reducing bacteria and extracellular polymeric substance-producing bacteria; the composite functional stabilizer is one or more of iron source minerals or ferrous salts and layered double hydroxides; and the auxiliary microbial agent is an electron donor bacteria and an anaerobic alkali-tolerant bacteria. The preparation method comprises the following steps: dry mixing dry components in the solidified material, adding the coastal soft soil, water and the biological agent into the dry mixture to form the fluid solidified soil, pumping, self-flowing or pouring construction, and curing under the conditions of a temperature of 10-40 DEG C and a water content of not less than 25% for 7-28 days. The sulfate ion and the chloride ion in the coastal soft soil are converted into stable components by the biological agent.

The invention relates to an economic process of producing extracellular polymeric substances (EPS), said process comprising: • contacting an aqueous stream comprising a carbon source with a biofilm generating system (BGS) under aerobic conditions to develop an EPS-containing biofilm on the BGS, the BGS comprising (i) an inert carrier and (ii) EPS-producing bacteria attached to the surface of the inert carrier; and • subjecting the BGS comprising the EPS-containing biofilm to a harvesting step in which the EPS-containing biofilm is separated from the inert carrier, the separated biofilm having an EPS content of at least 30% by weight of the organic matter that is contained in the separated biofilm; wherein the BGS is provided in the form of discrete pieces comprising an inert carrier body having a diameter in the range of 0.2-30 cm or wherein the BGS is provided in the form of a layer comprising a sheet of inert carrier.

The application discloses a method for improving in-situ bacteria and algae biofilm formation and nitrogen and phosphorus removal efficiency by high light intensity synchronization. The method irradiates the suspended in-situ bacteria and algae and the suspended filler under high light intensity, carries out photoassimilation reaction, reduces the total interfacial energy of the suspended in-situ bacteria and algae, and excessively secretes extracellular polymeric substances. The application greatly improves the adhesion of the suspended in-situ bacteria and algae on the filler, promotes the initial colonization of the suspended in-situ bacteria and algae on the filler, and makes the biomass of the biofilm on the filler increase by an average of 548%. Meanwhile, the ammonia nitrogen and phosphorus removal rates of the in-situ bacteria and algae biofilm system are increased by 72.1% and 90.3% respectively. The method has important significance for promoting low-carbon resource treatment of sewage.

This invention provides a silver-supported bismuth vanadate composite material, its preparation method, and its application, belonging to the field of photoelectrocatalysis technology. This invention utilizes Bacillus-based extracellular polymeric substances to reduce silver ions, obtaining silver nanoparticles, which are then used as co-catalysts to modify bismuth vanadate, resulting in a composite photoanode material for photoelectrochemical water splitting under natural light. The prepared silver-supported bismuth vanadate composite material exhibits excellent catalytic activity and stability. Compared to bismuth-based composite photoanode materials prepared using inorganic methods, the photoanode modified with microbial silver nanoparticles in this invention demonstrates superior OER performance under simulated sunlight, exhibiting better stability.

The application discloses a method for improving biopurification efficiency of marsh gas based on nano zero-valent iron composite material, which comprises the following steps: adding the nano zero-valent iron composite material into kitchen wastewater, adding anaerobic granular sludge into the mixed system, keeping the pH of the whole reaction system as 7.2+ / -0.2, blowing off for 5 minutes by using nitrogen to keep the anaerobic environment, and collecting the generated marsh gas by using a gas collection bag; wherein the nano zero-valent iron composite material comprises nano zero-valent iron coated by extracellular polymeric substance and / or nano zero-valent iron coated by sodium dodecyl sulfate. The application utilizes the kitchen wastewater to produce methane by anaerobic fermentation, adds the nano zero-valent iron composite material into the anaerobic system, provides H2 for hydrogenophilic methane-producing bacteria by corrosion and hydrogen evolution of the nano zero-valent iron composite material, and further improves the calorific value of the marsh gas.

This invention provides an analytical method for the extracellular polymeric carbon synthesis rate and carbon use efficiency, relating to the technical field of analytical testing. The method includes: pre-culturing sampled soil; adding an isotope with a purity of 98.0 at% 18 O-H2O, making the soil 18 A marker group was defined as having an O-H2O content of 20 at%; a group without added isotopes was also included. 18 The control group was treated with O-H2O. Extracellular polymers were extracted from the soil of both the labeled and control groups using a cation exchange resin method. After separation and purification, purified polysaccharides and proteins were obtained. Isotope mass spectrometry was used to determine the concentrations of O-H2O in the purified polysaccharide and protein samples. 18 The abundance of O and total oxygen content were used to calculate the atomic percentage of purified polysaccharides and proteins, respectively; the yield of purified polysaccharides and proteins was calculated; and the carbon synthesis rate and carbon utilization efficiency of extracellular polymers were calculated.

The application discloses a method for controlling membrane pollution by phage induction based on electrochemical regulation and application thereof, and relates to the field of environmental protection. 2+ The method comprises the following steps: adopting a low-voltage direct-current double-electrode system, fixing a membrane assembly with a biological pollution layer attached to the surface between an anode and a cathode, adding Ca 2+ solution into an electrolyte, applying a direct-current voltage and keeping low-speed stirring, so as to induce the protophages in the bacteria on the membrane surface to enter a lysis cycle, release the phage progeny and lyse other host bacteria, realize effective removal of the pollution layer on the membrane surface through the double synergistic effect of electrochemical oxidation degradation of extracellular polymeric substances and phage biological lysis. The application does not need to add exogenous virulent phages or chemical inducers, has the advantages of green environmental protection, low energy consumption, simple operation, high process integration degree, easy compatibility with an existing membrane system and the like, and can be widely applied to the fields of membrane biological pollution control in water treatment and membrane separation.

A method for enhancing the operation of a biofilm reactor using extracellular polymers involves adding SLB-EPS (soluble extracellular polymer SB-EPS and loosely bound extracellular polymer LB-EPS) and tightly bound EPS (TB-EPS) extracted from waste activated sludge to the biofilm reactor during both the start-up and operation phases. The different amounts of PS and PN components in the EPS promote the initial and stable adhesion of negatively charged microorganisms to the carrier surface, achieving rapid start-up and long-term efficient and stable operation of the biofilm reactor. Simultaneously, it realizes the resource utilization of waste sludge, reduces sludge disposal costs, integrates wastewater treatment plants into the circular economy concept, and responds to the carbon neutrality principle. This invention is applicable to different types of biofilm reactors and packing materials, has a wide range of applications, and has significant potential for widespread application.

This invention relates to a bacterial strain that improves the physical structure of red mud and its application. The strain BRT1 is classified as Staphylococcus epidermidis. Staphylococcus epidermidis The accession number is GDMCC No. 66375, the accession date is May 22, 2025, and the depositary institution is Guangdong Microbial Culture Collection Center. This invention utilizes the BRT1 strain, which efficiently improves the physical structure of red mud, solving the technical problem of poor red mud structure. The BRT1 strain secretes extracellular polymeric substances (EPS) as a bio-cementitious agent, effectively promoting the aggregation of red mud particles and forming stable aggregate structures.

The application provides a method for sludge dewatering by combining biological enzyme and deep eutectic solvent, comprising: adding biological enzyme to sludge for hydrolysis to obtain enzyme-hydrolyzed sludge, adding deep eutectic solvent to the enzyme-hydrolyzed sludge for conditioning, and mechanically dewatering the conditioned sludge, wherein the deep eutectic solvent comprises choline chloride and organic acid. The method can shorten the capillary water absorption time and reduce the specific resistance of filter cake by first hydrolyzing to destroy the extracellular polymeric substance (EPS) network of sludge and then using DES for deep modification and hydrophobic reconstruction, and can also significantly reduce the water content of the sludge cake.

This invention discloses a method for alleviating bioclogging through the synergistic degradation of extracellular polymers by multiple enzymes, comprising: selecting freeze-dried *Pseudomonas aeruginosa* and reviving and culturing it to obtain bacterial solution a; expanding the reviving bacterial solution a and mixing it to obtain bacterial solution b; re-inoculating bacterial solution b into a new culture medium until the logarithmic growth phase to obtain bacterial solution c; centrifuging bacterial solution c in the logarithmic growth phase and adding MOPS buffer solution to the precipitate to obtain a bacterial suspension; preparing a compound enzyme agent and adding the prepared compound enzyme agent to the bacterial suspension to react and decompose the extracellular polymers secreted by the microorganisms, thereby solving the clogging problem. This invention can alleviate the formation of bioclogging without affecting the water quality of reinjected water. Compared with single enzymes or combinations of two enzymes, it has advantages such as lower dosage, lower cost, better effect, and faster results.

The present invention is in the field of organic macromolecular compounds, their preparation, and compositions based thereon, in particular biobased extracellular polymeric substances, a method of forming said biobased extracellular polymeric substances, and a composite material comprising said biobased extracellular polymeric substances.