95 results about "Extracellular polymeric substance" patented technology

The invention discloses an SRB remediation system capable of stably operating for treating heavy metal wastewater for a long time and application, belongs to the technical field of heavy metal wastewater treatment, and solves the problems that in the prior art, mining heavy metal wastewater generally contains high-concentration soluble heavy metal, high sulfate content and low pH value, the ecological environment is seriously damaged, and the service life of the mining heavy metal wastewater is prolonged. And the traditional physicochemical treatment method has the technical problems of high agent consumption, high sludge amount, easiness in secondary pollution and the like. According to the SRB remediation system capable of stably operating for a long time and used for treating heavy metal wastewater, efficient removal and immobilization of heavy metal are achieved on the basis of the biological vulcanization effect of SRB composite flora in a UASB bioreactor in combination with the synergistic effect of microbial communities and the protection mechanism of extracellular polymeric substances, and by constructing the composite SRB flora system, operation parameters are accurately regulated and controlled, so that the heavy metal wastewater treatment efficiency is improved. And efficient and stable removal and fixation of various heavy metals in the AMD are realized in the UASB reactor by utilizing a cooperative protection mechanism of EPS.

The invention belongs to the technical field of sewage treatment, and discloses a DO-ORP coordinated regulation artificial tidal food web sludge in-situ reduction and phosphorus recovery method. Aiming at the problems of high energy consumption, serious phosphorus resource loss and the like of traditional sludge treatment, a multi-stage food chain consisting of microorganisms, protozoa and metazoan is implanted by constructing an artificial tidal environment with periodic alternation of wetting and drying; according to the method, the metabolic activity of sludge hydrolysis flora and phosphorus-accumulating bacteria is activated by utilizing cooperative regulation and control of DO concentration and ORP, microbial extracellular polymeric substances are triggered to be depolymerized by adopting tidal periodic oscillation, in-situ reduction of sludge biomass is realized by combining a food web cascade predation effect, and phosphorus is synchronously removed by virtue of an anaerobic phosphorus release-aerobic phosphorus uptake coupled struvite crystallization process. The method is suitable for treating the excess sludge of a municipal sewage treatment plant, and the high-purity magnesium ammonium phosphate fertilizer is produced while the dehydration energy consumption is reduced by 40%.

The invention discloses a probiotic strain Bifidobacterium animalis subsp. Lactiss S8 of a high-yield biological membrane, the preservation number is GDMCC NO.66518, the characteristics of the stable high-yield biological membrane are obtained through directional screening and adaptive evolution, and the yield of the biological membrane is three times higher than that of a positive control. The extracellular polymeric substance (EPS) of the strain can significantly enhance the tolerance of the strain to severe environments such as gastric acid, cholate and the like, and improve the intestinal colonization ability. Experiments prove that the strain remarkably reduces two liver function indexes, namely ALT and AST, in an animal model, and shows that the strain has a certain effect of improving liver injury.

The invention belongs to the field of microorganisms, and particularly discloses paenibacillus mucilaginosus JZ1 and application thereof. According to the invention, paenibacillus mucilaginosus with multifunctional characteristics is screened from biological crust. The paenibacillus mucilaginosus JZ1 strain obtained by the invention has multiple functions of dissolving phosphorus, dissolving potassium, fixing nitrogen, and producing IAA, ACC deaminase, siderophore and extracellular polymeric substance (ESP), is an environment-friendly biological material, and does not cause environmental pollution. The bacterial strain is combined with the crust algae for application, so that the effects of promoting plant growth and improving soil can be stably exerted. The method has a relatively great application prospect.

The application provides a sludge immobilization-based gel aerobic particle and a preparation method and application thereof, and belongs to the field of sewage treatment.The method comprises the following steps: 1, uniformly dispersing sEPS powder in a sodium alginate solution to obtain a composite gel; 2, filtering, centrifuging and removing supernatant of activated sludge in an aerobic tank to obtain concentrated bacteria solution; 3, adding the concentrated bacteria solution into the composite gel, uniformly mixing, dropping into a cross-linking solution, and then solidifying to obtain the gel aerobic particle.The sludge immobilization-based gel aerobic particle is prepared by using structural extracellular polymeric substances in activated sludge and sodium alginate to prepare a composite gel, and using a multivalent metal ion cross-linking agent to immobilize concentrated activated sludge, so that the gel aerobic particle with similar chemical composition, mechanical strength and biological performance to natural aerobic particles is prepared, and rapid granulation of sludge and rapid start-up operation of a granular system are realized.

This invention relates to the field of catalytic degradation technology for organic pollutants, and particularly to an iron-modified biochar immobilized catalase, its preparation method, and its application. The preparation method of the iron-modified biochar immobilized catalase includes the following steps: mixing iron-modified biochar, catalase solution, and an extracellular polymeric substance; performing an immobilization and cross-linking reaction; and separating the solid and liquid phases to obtain the iron-modified biochar immobilized catalase; the extracellular polymeric substance is secreted by *Goldenella*. This invention uses an extracellular polymeric substance instead of traditional glutaraldehyde as a cross-linking agent, avoiding the hazards associated with glutaraldehyde. Simultaneously, the prepared iron-modified biochar immobilized catalase exhibits higher enzyme activity and superior pollutant degradation performance.

The application discloses a kind of microbial flocculants and preparation method thereof, the preparation method includes the following steps: camellia cake meal is mixed with water, ultrasonic, hydrothermal treatment, filtration, filtrate is collected, and camellia cake meal extraction solution is obtained, configuration is camellia cake meal culture medium, inoculation flocculation function bacteria seed liquid is cultivated, and the preparation of microbial flocculants is completed.In the application, the way of ultrasonic extraction and hydrothermal extraction is combined, and camellia cake meal extraction solution with rich nutritional ingredients and good adaptability of various nutrients can be prepared, which can promote the rapid growth of flocculation function bacteria when used for culturing flocculation function bacteria, increase biomass, and induce more extracellular polymeric substance, thereby obtaining microbial flocculants with high yield and high flocculation rate.In the application, camellia cake meal is used as raw material, which can greatly reduce production cost and simplify production steps, promote large-scale use of microbial flocculants, and realize resource utilization of camellia cake meal.

The invention discloses a multistage enzyme and quorum sensing signal molecule MABR membrane and a preparation method thereof, through a bionic induction strategy, while the anti-pollution characteristic of the MABR membrane is maintained, multistage enzyme and quorum sensing signal molecules are fixed on the surface of the membrane, and the multistage enzyme and quorum sensing signal molecules induce microorganisms to secrete an extracellular polymeric substance (EPS), so that the binding strength of a biological membrane is improved by 40% or more. The preparation method of the multi-stage enzyme and quorum sensing signal molecule MABR membrane comprises the following steps: (1) screening nitrifying bacteria and extracting multi-stage enzyme and quorum sensing signal molecules of the nitrifying bacteria; (2) carrying out composite pretreatment on the surface of the MABR membrane, wherein the composite pretreatment comprises hydrophilic modification, functional group introduction, swelling of the surface of membrane silk and the like; and (3) immobilizing multistage enzyme and quorum sensing signal molecules (including cell membrane fragments, periplasmic space enzyme complexes and the like) in the strain by adopting an immobilization technology to form a bioactive interface layer with the thickness of about 50-200nm.

The application belongs to the technical field of microbial sewage treatment, and particularly relates to an iron-carbon quantum dot / electrically active non-oxygen-producing photosynthetic bacteria / carrier hybrid cell factory and application thereof in sewage resourceization or carbon emission reduction; the iron-carbon quantum dot in the hybrid cell factory provided by the application promotes the non-oxygen-producing purple photosynthetic bacteria to utilize more light energy, and generates photo-generated electrons as an extracellular efficient electron donor source, and the photosensitive active substances in the extracellular polymeric substance of the non-oxygen-producing purple photosynthetic bacteria can promote the formation of a continuous cycle of trivalent iron and divalent iron, realize mutual cooperation, promote sewage resourceization and carbon emission reduction, and synthesize high-value-added products; and in addition to facilitating the recovery of biomass, the transparent conductive glass electrode as the carrier also ensures that the non-oxygen-producing purple photosynthetic bacteria stably and efficiently utilize light energy; therefore, the hybrid cell factory provided by the application is a high-efficiency microbial sewage treatment technology, and solves the technical problem of lack of high-efficiency microbial sewage treatment technology in the prior art.

The invention belongs to the technical field of sludge treatment, and provides a method for strengthening anaerobic fermentation of sludge by activated sodium pyrosulfite, which comprises the following steps: pretreating sludge to obtain a sludge system; adding ferric salt and sodium pyrosulfite into the sludge system to obtain an activation system; and performing anaerobic fermentation on the active system to finish the treatment. By constructing a Fe < 3 + > / sodium pyrosulfite free radical system, the dissolution of organic matters in the sludge is improved, so that the generation of short-chain fatty acid (SCFAs) in the anaerobic fermentation process is promoted. Sodium pyrosulfite is dissolved in water to form sulfite species, under the action of ferric ions, the sulfite species can generate various active free radicals through electron transfer, and the free radicals have high oxidation capacity and can destroy sludge floc structures and extracellular polymeric substances, so that cell lysis is promoted, intracellular organic substances are released, and the sludge can be degraded. The concentration of a substrate available for anaerobic fermentation is improved, and the yield of short-chain acid is substantially improved.

The present application belongs to the field of sewage biological treatment technology, and particularly relates to a method for extracting different extracellular polymer components from activated sludge. The method focuses on layered extraction of various components of different extracellular polymers, that is, S-EPS in the activated sludge is first extracted by centrifugation, then LB-EPS is extracted by ultrasonic method and high-speed centrifugation, ion TB-EPS is extracted by EDTA method and high-speed centrifugation, and finally, hydrophobic TB-EPS is extracted by ethanol method and high-speed centrifugation. The combination of the above methods can complete the extraction of different components of extracellular polymers. The method for extracting different extracellular polymer components from activated sludge provided by the present application can ensure high extraction efficiency and high purity of EPS, and at the same time, the influence of the extraction method on cell lysis is as small as possible, and the quantitative accuracy and feasibility of the extracellular polymer extraction method are ensured.

This invention discloses a method for extracting extracellular polymers from benthic microalgae culture medium, as well as the products and applications thereof, comprising the following steps: adding formamide to the benthic microalgae culture medium, adding Na... + The extracellular polymeric substance (ECPL) is obtained by stirring, centrifuging, heating and concentrating, freeze-drying, dissolving in water, adding anhydrous ethanol, precipitating, dissolving again in water, dialyzing, and freeze-drying. This invention improves upon existing ethanol precipitation processes by employing an extraction process of "heating and concentrating benthic microalgae culture medium—freeze-drying to obtain a crude extract—redissolving the crude extract in water—precipitating with anhydrous ethanol—dissolving the precipitate—dialysis—freeze-drying to obtain extracellular polymeric substances." Compared to traditional extraction processes, this method allows for "controllable" ethanol dosage by dissolving a large amount of crude extract in a small amount of water to obtain a solution rich in extracellular polymeric substances. Furthermore, it significantly reduces the amount of anhydrous ethanol used when extracting large quantities of extracellular polymeric substances, greatly lowering experimental costs.

The invention discloses a method for promoting sludge dewatering by utilizing low-temperature heating under an acidic condition in cooperation with POM (polyoxymethylene), which comprises the following steps: firstly, heating activated sludge to be treated to a set temperature, adding phosphomolybdic acid into the activated sludge under a stirring condition, then adding dilute sulphuric acid to adjust the pH value to 2.0-7.0, and reacting under the stirring condition, according to the method disclosed by the invention, phosphomolybdic acid and low-temperature heat are used for cooperatively treating the sludge, and through the synergistic effect of acidic catalysis and thermally induced structure relaxation, the oxidative cracking of an extracellular polymeric substance (EPS) and the dissolution of organic matters in sludge floc are synchronously realized; the technical bottlenecks that the reaction path is limited and the energy utilization efficiency is low in the traditional single chemical oxidation or thermal conditioning process are broken through.

This invention relates to a method for the simultaneous capture of carbon and phosphorus in wastewater. It addresses the problems of high aeration energy consumption, long hydraulic retention time, low phosphorus removal rate, and high carbon, ammonia, and phosphorus levels in existing wastewater treatment processes. The method utilizes the aerobic phosphorus absorption characteristics of microorganisms, employing tightly bound EPS (extracellular polymeric substances) to rapidly store and release phosphorus, capturing it in the sludge phase. This simultaneously and rapidly reduces COD and total phosphorus in the wastewater to low levels, achieving a COD removal rate of over 70%. Furthermore, the proportion of carbon captured and converted into the sludge phase reaches 30%–37%, while the proportion of organic matter oxidized to CO2 is only 22%–31%, resulting in an effective phosphorus capture rate of 70%–75%, achieving highly efficient capture and removal of both phosphorus and organic matter. Simultaneously, for effluent treatment with irrigation potential, the method achieves phosphorus capture ratio control based on efficient carbon capture, thereby obtaining effluent with low COD concentration and dynamically adjustable nitrogen-to-phosphorus ratio to meet target irrigation needs.

The invention belongs to the technical field of sludge treatment, and provides a method and a system for strengthening sludge cracking and producing medium-chain fatty acid. The method comprises the following steps: concentrating sludge, mixing the concentrated sludge with halogenated hydantoin, and cracking to obtain cracked sludge; and carrying out two-stage fermentation treatment on the cracked sludge to obtain the medium-chain fatty acid. According to the method, halogenated hydantoin is adopted as a pretreatment agent for cracking the sludge, and through spontaneous combination of halogenated hydantoin molecules and the sludge extracellular polymeric substances, tight combination type extracellular polymeric substances and cell walls are remarkably oxidized and damaged; meanwhile, hypochlorous acid is slowly released to maintain a proper oxidative stress effect to stimulate the biological metabolism effect of anaerobic microorganisms, hydrolysis and acidification of organic matters are promoted, and a large number of electron acceptors are provided for microorganisms participating in carbon chain extension to produce MCFA subsequently. The method is simple, low in cost, small in medicament demand and beneficial to promoting efficient resource utilization of the sludge.

The invention belongs to the technical field of sewage treatment, and discloses a polyacrylamide compounded inorganic matter sludge treatment agent and a preparation method, and the polyacrylamide compounded inorganic matter sludge treatment agent comprises the following components by mass: 5-20 parts of polyacrylamide, 20-30 parts of straw charcoal, 30-40 parts of an inorganic flocculant, and 10-30 parts of a thickener. The long-chain bridging effect of the polyacrylamide and the charge neutralization effect of the inorganic flocculant jointly compress the double electric layers of the sludge colloid; the porous structure of the straw biochar and the water retention regulation of the thickening agent optimize the permeability of the filter cake; the pH regulation of the quicklime cooperates with the thickening agent to crack the extracellular polymeric barrier, so that the quicklime can improve the dewatering performance of sludge and reduce the water content of the sludge when being applied to sludge treatment, and has good social and economic benefits.

The present application relates to a kind of reverse osmosis membrane pollution control method based on green biological scale inhibition, especially suitable for reverse osmosis membrane pollution treatment in industrial wastewater zero discharge system.The method comprises: regulating the composition of influent organic components by sand filter pretreatment unit, using the microbial community and its extracellular polymeric substance naturally enriched in sand filter process, without additional bacteria or chemical scale inhibitor, can directly interfere with the nucleation and crystallization process of inorganic scale on membrane surface, to form loose reversible pollution layer structure.The method effectively reduces the scale rate of inorganic salts such as calcium carbonate, inhibits membrane surface biological pollution, delays the densification of pollution layer, improves membrane flux retention rate, prolongs membrane operation cycle, reduces cleaning frequency.Compared with the existing conventional physical or chemical pretreatment method, the present application makes full use of the natural scale inhibition effect of the ecological system in sand filter unit, has the advantages of low operating cost, environmental friendly, strong adaptability and the like.

The invention provides a gel aerobic particle based on sludge immobilization and a preparation method and application thereof, and belongs to the field of sewage treatment.The method comprises the steps that 1, sEPS powder is evenly dispersed in a sodium alginate solution, and composite gel is obtained; 2, filtering and centrifuging the activated sludge in the aerobic tank, and removing supernate to obtain concentrated bacterial liquid; and 3, adding the concentrated bacterial liquid into the composite gel, uniformly mixing, dripping into a cross-linking solution, and curing to obtain the gel aerobic particles. According to the gel aerobic particle based on sludge immobilization, the composite gel is prepared from the structural extracellular polymeric substance in the activated sludge and the sodium alginate, and the concentrated activated sludge is immobilized by using the multivalent metal ion cross-linking agent; gel aerobic particles with chemical components, mechanical strength and biological properties similar to those of natural aerobic particles are prepared, and rapid granulation of sludge and rapid starting operation of a granular system are achieved.

The invention provides a composite spray lotion of a targeted vaginal pathogenic bacterium biological membrane and an ultrasonic infiltration promotion system. The spray lotion comprises the following components in parts by weight: 0.1-5 parts of antibacterial peptide; 10 to 100 parts of bacteriophage; 30 to 80 parts of rose water; 10 to 30 parts of betaine; 20 to 50 parts of cocamidopropyl betaine; 5 to 20 parts of lauryl glucoside; 5 to 20 parts of sodium lauroyl sarcosinate; 0.2 to 1 part of chlorhexidine acetate; and 500 to 1000 parts of purified water. The antibacterial peptide of the spray-washing liquid directly acts on extracellular polymeric substances of vagina biomembranes, the compact structure of the extracellular polymeric substances is destroyed, and a permeation channel is opened for subsequent antibacterial components; meanwhile, pathogenic bacteria in vaginal biomembranes can be directly killed, the action mechanism of the antibacterial peptide is that bacterial cell membranes are physically damaged, and the pathogenic bacteria are not prone to generating drug resistance. The components such as the rose water can relieve discomfort such as pruritus, dryness and scorching hot caused by vaginal infection or improper cleaning, and are suitable for treatment scenes such as colpitis mycotica and abnormal leucorrhea, high in safety and wide in applicability.

The present application belongs to the technical field of sewage treatment, and discloses a method for simultaneously producing medium-chain fatty acids and hydrogen by enhancing sludge anaerobic fermentation. The sludge is concentrated and mixed with alkaline biochar, and then potassium ferrate is added for reaction to obtain pretreated sludge. After adjusting the pH, the sludge is mixed with an electron donor and inoculated sludge for anaerobic fermentation to obtain a fermentation liquor rich in medium-chain fatty acids and hydrogen. The alkaline biochar-potassium ferrate coupling system is used to break down the extracellular polymeric substance and cell wall of the sludge, drive the dissolution of particulate organic matter, and inhibit the activity of methanogens. Fe2O3 derived from potassium ferrate forms an iron-carbon composite with biochar in situ, and by enhancing the efficiency of electron transfer and enzyme activity, the acidification process of organic matter and the carbon chain extension reaction are promoted, forming a composite technology with three functions of "pretreatment-electron regulation-methane inhibition", realizing the simultaneous recovery of medium-chain fatty acids and hydrogen, and improving the sludge treatment efficiency.

The invention belongs to the technical field of environment detection, and particularly relates to a method for detecting Cu < 2 + > in a water body based on a biosynthetic cadmium sulfide quantum dot fluorescent probe. Cadmium sulfide quantum dots biosynthesized by an extracellular polymeric substance (EPS) are used as a fluorescent probe for detecting Cu < 2 + >, specifically, EPS is generated through carbon source stress / induction of sulfur vibrio desulfurized subspecies, the cadmium sulfide quantum dots with strong fluorescence are synthesized in the EPS, then the cadmium sulfide quantum dots and a buffer solution are mixed to prepare a fluorescent probe solution, and the fluorescent probe is used for detecting Cu < 2 + >. The obtained cadmium sulfide quantum dot fluorescent probe is used for detecting Cu < 2 + > in a water body. The method for detecting Cu < 2 + > has the advantages of simplicity in operation, high sensitivity, low detection limit and good selectivity, and has good quantitative detection capability on Cu < 2 + > in actual water, and the cadmium sulfide quantum dots are simple in synthesis, relatively low in cost and environment-friendly.

The invention discloses a method for extracting an extracellular polymeric substance from cladophora, belongs to the field of biotechnology and natural product extraction, and particularly relates to a method for extracting the extracellular polymeric substance from cladophora by culturing initial cladophora through a microalgae culture medium to obtain a cladophora frond and then extracting the cladophora frond through an extraction process to obtain the extracellular polymeric substance. The extraction process comprises a close binding type EPS extraction process, the close binding type EPS extraction process adopts temperature gradient control enzyme-heat synergistic extraction, the extraction temperature is 20-40 DEG C, and the enzyme is cellulase. The method for extracting the extracellular polymeric substance from cladophora has the advantages of high EPS extraction amount, high polysaccharide content in EPS, high biological activity and good extraction effect.

The invention discloses a method for enhancing denitrification of nano-iron adaptive microorganisms, and belongs to the technical field of water pollution control. The method comprises the following steps: preparing extracellular polymeric substance (EPS) and iron sulfide co-doped modified nano-iron (E (at) SnZVI) through a mechanical ball milling method or a liquid phase chemical reduction-co-precipitation method; the method comprises the following steps: adjusting the pH value of nitrate-containing wastewater to be treated to 5.0-7.0; (2) adding the E (at) SnZVI prepared in the step (1) into the wastewater according to the adding amount of 150 mg / L, inoculating domesticated denitrifying bacteria mixed liquor (MLSS is 3000 mg / L), and controlling the C / N ratio of the system to be 2-5; reacting under an anoxic condition, and monitoring the concentration change of nitrate, nitrite, ammonia nitrogen and nitrogen in the system until the reaction is balanced. In the wastewater with low carbon-nitrogen ratio (C / N = 2), the reaction period is shortened from more than 24 hours to 4-8 hours, and the method is suitable for a continuous flow treatment process. EPS is natural secreta of microorganisms, FeSx is low in toxicity, reaction products are iron oxide and nitrogen, and secondary pollution is avoided; no additional carbon source is needed, and the policy requirements of pollution reduction and carbon reduction are met.

The invention provides a method for evaluating an activated sludge system response mechanism under antibiotic impact and application thereof, and belongs to the technical field of water environment risk evaluation and management. The method mainly relates to detection of water quality indexes (ammonia nitrogen, total nitrogen, total phosphorus and chemical oxygen demand) in the activated sludge and physicochemical characteristics (extracellular polymeric substance EPS, sludge settling performance and Zeta potential) of the activated sludge, and a response mechanism of an activated sludge system under antibiotic impact is analyzed by taking ciprofloxacin (CIP), ofloxacin (OFL) and enrofloxacin (ENR) as indication pollutants. Effective reference is provided for system regulation and control when a sewage plant faces antibiotic impact, and a new insight is provided for removing antibiotics and drug-resistant genes in wastewater by explaining the relation among functional genes, drug-resistant genes and microbial communities in an activated sludge system under antibiotic exposure.

The invention discloses a soil extracellular polymeric substance extraction and determination method based on a cation exchange resin method, and relates to the technical field of soil analysis, and the method specifically comprises the following steps: firstly, cleaning cation exchange resin with hot water, then sequentially adding HC l, NaC l and NaOH solutions, oscillating, and airing to obtain CER resin particles; the method comprises the following steps: treating a fresh soil sample by using a CaC12 solution and a buffer solution to obtain a pretreated soil sample; weighing CER resin particles, adding the CER resin particles into a centrifugal tube filled with the pretreated soil sample, oscillating, centrifuging and filtering to obtain an EPS extracting solution; and finally, detecting the absorbance of the EPS extracting solution under different wavelengths by using a microplate reader to respectively obtain the polysaccharide content and the protein content. According to the EPS extraction and determination method, the extraction efficiency of the EPS in the soil is effectively improved by optimizing the pretreatment steps of the cation exchange resin and accurately controlling parameter conditions such as the resin dosage, the extraction time and the rotating speed.

This invention relates to the field of nanomaterials technology, and discloses a method for preparing and applying gold nanoparticles synthesized using microbial extracellular polymers (EPS). The method uses methylbazin exopolymers (EPS) as a green reducing agent and stabilizer, reacting them with chloroauric acid in an aqueous phase. By adjusting the EPS concentration, pH, and temperature, gold nanoparticles (EPS-Au NPs) can be synthesized in a controlled manner. The resulting nanoparticles exhibit high crystallinity and good dispersibility, and demonstrate ultra-high activity in catalyzing the reduction of 4-nitrophenol, with a TOF value reaching 485.53 h⁻¹. ‑1 This invention is superior to many traditional catalysts and has good cycle stability. The process of this invention is green and controllable, and the resulting catalyst has promising applications in wastewater treatment and other fields.

The invention discloses a culture method for inducing mycelia to secrete specific extracellular polymeric substances and application of the culture method in sand stabilization, and relates to the field of biotechnology and environmental materials.The culture method comprises the following steps that phanerochaete chrysosporium is inoculated into a liquid fermentation culture medium, and fermentation is conducted under the conditions that the pH ranges from 4.5 to 5.5, the temperature ranges from 35 DEG C to 37 DEG C and shaking culture is conducted; the liquid fermentation culture medium contains tween-80 with the concentration of 0.5-4 g / L and hydrogen peroxide with the concentration of 0.05-0.15 mM as an inducer combination; after the fermentation is finished, separating to obtain fermentation supernate, namely the functional extracellular polymeric substance fermentation liquor; according to the culture method for inducing the mycelium to secrete the specific extracellular polymeric substance and the application of the culture method in sand stabilization, the functional EPS is obtained from the source of regulating and controlling microbial metabolism, and the limitation that a traditional mycelium material only depends on the physical form is broken through.

The invention discloses a clarification and separation system integrated with a biological filtration function and a treatment method of the clarification and separation system, and belongs to the technical field of sewage treatment. The system comprises a clarification separator body, a water distribution device and a water collection weir groove, the interior of the clarification separator body is sequentially divided into a sludge settling area, a diversion area, a biological filtration area, a clarification area and an ultrahigh area from bottom to top. The flow guide plate is used for guiding the flow of the mixed liquid, so that large-particle sludge is settled, fine sludge is guided to float upwards, and a sludge floc filtering layer with a certain thickness is naturally formed in the biological filtering area; a porous net-shaped structure formed by sludge flocs and extracellular polymeric substances (EPS) in the filter layer is used for permeating, intercepting, adsorbing and purifying rising water flow. The integrated treatment of precipitation separation and biological filtration is realized, an advanced treatment unit does not need to be additionally arranged, the SS (suspended solids) in the effluent is stable and less than or equal to 10mg / L, and the integrated treatment device has the advantages of small occupied area, low operation energy consumption, adaptability to high-sludge-concentration working conditions and the like.

The invention provides an autotrophic nitrogen removal reactor as well as a preparation method and application thereof, and belongs to the technical field of biological sewage treatment. According to the invention, the ammonia nitrogen adsorption material is mounted in the suspension balls to form the porous spherical filler, the adsorption capacity of ammonia nitrogen is improved, a large number of biological membranes are formed on the porous spherical filler through biological domestication, and the biological membranes mainly comprise nitrosobacteria and anaerobic ammonium oxidation bacteria, so that the adsorption capacity of ammonia nitrogen is improved. Due to the fact that nitrosobacteria need to consume a large amount of dissolved oxygen in the ammonia nitrogen conversion process, an anoxic or even anaerobic environment exists on the surface of a biological membrane, basic conditions are provided for growth of anaerobic ammonium oxidation bacteria on the biological membrane, and the anaerobic ammonium oxidation bacteria can secrete a large amount of extracellular polymeric substances in the metabolism denitrification process; and the anaerobic microenvironment is gradually expanded, so that by controlling the concentration of dissolved oxygen in water, the co-growth of the two florae can be simultaneously maintained on a biological membrane on the surface of the zeolite, the conversion and removal of ammonia nitrogen are jointly completed, and a synergistic denitrification effect is formed.

The invention discloses a method for constructing and reinforcing a hybrid biological membrane system composed of methane bacteria / carbon nitride. According to the method, a hybrid system composed of methanogens and an extracellular polymeric substance (EPS) semiconductor material (modified graphite-phase carbon nitride) is constructed, and the hybrid system is used for preparing the carbon nitride-modified graphite-phase carbon nitride hybrid system. The carbon nitride-modified graphite-phase carbon nitride hybrid system has the advantages that the carbon nitride-modified graphite-phase carbon nitride hybrid system is used for preparing the carbon nitride-modified graphite-phase carbon nitride hybrid system, and the carbon nitride-modified graphite-phase carbon nitride hybrid system; the extracellular polymeric substance in the biological membrane obviously improves the light energy utilization efficiency and the operation stability of the system; nano conductive particles are introduced to the surfaces of methane bacteria cells, so that the utilization rate of extracellular electrons by the cells is improved. The method effectively solves the problems of low efficiency, high energy consumption, low product selectivity and the like in the process of reducing carbon dioxide into methane in a traditional biological hybrid system, and has the advantages of simple and convenient process, low energy consumption and high product selectivity.