144 results about "Extracellular polymeric substance" patented technology

The invention provides a magnetite-loaded extracellular polymeric material and a preparation method and application thereof.The preparation method comprises the following steps that excess sludge and a sodium chloride solution are mixed and heated, extracellular polymeric substances in the excess sludge are dissociated, and a mixed solution is obtained; carrying out centrifugal treatment on the mixed solution, and filtering supernate through a filter membrane to obtain an extracellular polymeric substance mixed solution; adding magnetite powder into the extracellular polymer mixed solution, stirring under the ultrasonic action until the powder is completely dissolved, adjusting the pH to be neutral, and performing vacuum freeze drying to obtain a first product; mixing the first product, sodium alginate, polyvinyl alcohol and water, and then dropwise adding a calcium chloride solution for gelation treatment to obtain hydrogel beads; and carrying out vacuum freeze drying on the hydrogel beads to obtain the extracellular polymer hydrogel material loaded with magnetite. The material can effectively and stably promote granulation of sludge, effectively solves the recycling problem of excess sludge, and is beneficial to reduction of production cost.

The invention discloses a method for promoting anaerobic fermentation of excess sludge to produce short-chain fatty acid, and relates to the field of environmental protection, and the method comprises the following steps: naturally settling the excess sludge, and removing supernate to obtain concentrated sludge; the method comprises the following steps: firstly, adding ammonium persulfate into concentrated sludge to completely dissolve the concentrated sludge, then adding alkali, sealing and placing the concentrated sludge in a constant-temperature shaking table for full reaction, and obtaining sludge after the reaction is finished as pretreated sludge; the pretreated sludge is directly used as a substrate and a fermentation product or inoculated sludge is used as a substrate, nitrogen is filled to expel oxygen, the mixture is sealed and then placed in a constant-temperature shaking table to be subjected to anaerobic fermentation to produce short-chain fatty acid, and pH is not adjusted in the anaerobic fermentation process. According to the method, high-activity free radicals and free ammonia are generated through reaction of alkali and ammonium persulfate to destroy extracellular polymeric substances and cells of the sludge, so that release of organic matters in the sludge is promoted, the yield of short-chain fatty acid in the sludge fermentation liquor is greatly increased, and the anaerobic fermentation time is remarkably shortened.

The invention discloses a preparation method and application of sludge-based biochar loaded with group sensing signal molecules (AHLs) and extracellular polymeric substances (EPS). The preparation method comprises the following steps: culturing, crushing and concentrating waste anaerobic granular sludge (AnGS) to extract AHLs; the method comprises the following steps: washing AnGS, heating in a water bath, and centrifugally extracting EPS; and then drying and grinding the AnGS, stirring in a constant-temperature water bath, pyrolyzing, washing and grinding to prepare biochar, and finally freeze-drying to load the AHLs and the EPS on the sludge-based biochar. The loaded sludge-based biochar can be used as a redox mediator, is applied to the field of anaerobic digestion of wastewater, and aims to improve extracellular electron transfer and enhance the anaerobic treatment efficiency of refractory wastewater. The method has the advantages that the prepared loaded sludge-based biochar has a good removal effect on organic pollutants, meanwhile, the waste activated sludge can be recycled, waste is treated with waste, environmental pollution is reduced, resources are recycled, and cost is saved. In addition, the method has good application value in the aspects of soil remediation, catalysis and the like.

The invention relates to a biological membrane sewage treatment method and device based on aeration on-off time intelligent regulation and control. The method comprises the following steps: sewage pretreatment: adding a flocculating agent into sewage of a flocculation device, stirring by stages, standing and precipitating, settling and separating floc, and enabling supernatant obtained by separation to enter a membrane bioreactor through a first water outlet device; aeration treatment: adopting an intermittent aeration mode to carry out cycle aeration on the supernatant conveyed by the first water outlet device; membrane separation treatment: pumping the sewage subjected to aeration treatment into a second water outlet device from the membrane bioreactor under the negative pressure pumping action of a centrifugal pump so as to realize the separation of the sewage and pollutants, and regulating and controlling the aeration on-off time according to the composition characteristics of microbial communities in the process of performing aeration treatment in an intermittent aeration mode so as to realize the aeration treatment. The invention relates to a method for optimizing the relative abundance of part of phylum and reducing the accumulation of extracellular polymeric substances and soluble microbial products. The device comprises a flocculation device, a membrane bioreactor and a control unit.

The invention discloses a method for improving uranium immobilization by bacillus by using an extracellular polymeric substance, and belongs to the field of environmental engineering, the bacillus licheniformis X-113 is separated from a uranium-polluted environment and is preserved in the China Center for Type Culture Collection (CCTCC) with the preservation number of CCTCC NO: M 20251207, and the bacillus licheniformis X-113 is preserved in the China Center for Type Culture Collection (CCTCC) with the preservation number of CCTCC NO: M 20251207. By optimizing the EPS culture conditions, the fixing capacity of the EPS-Bacillus sp.X-113 on uranium is remarkably enhanced compared with that of Without EPS-Bacillus sp.X-113, and the fixing capacity of the EPS-Bacillus sp.X-113 on uranium is remarkably enhanced compared with that of Without EPS-Bacillus sp.X-113. And numerous functional groups in EPS, such as carboxyl, hydroxyl, amino and the like, can be subjected to complexation, ion exchange and other reactions with uranium ions to fix the uranium ions, so that the uranium fixation of the bacillus is greatly improved.

The invention provides a device and a method for improving transparency of a natural water body by utilizing a microalgae biological membrane, and belongs to the field of water treatment, and the device comprises a water tank, rollers, a microalgae attachment matrix, a water distribution device and a lighting device. The transparency of the natural water body is effectively improved through the microalgae biological membrane. The microalgae biological membrane secretes macromolecular extracellular polymeric substances, can adsorb suspended particulate matters and soluble organic matters in water, and inhibits the reproduction of other planktons through nutrition competition, so that the turbidity is reduced. In addition, the device is designed as up-flow water inlet, so that the contact between the water body and the microalgae biological membrane is enhanced, and the treatment effect is improved. LED lamps and a heating device are adopted to keep a proper growth environment, and meanwhile, a photovoltaic power generation system is arranged, so that green and environment-friendly effects are achieved. The method is simple and easy to implement, low in cost and convenient to manage and maintain, and a novel and effective technical scheme is provided for improving the transparency of the natural water body.

The invention relates to a reverse osmosis membrane pollution control method based on green biological scale inhibition, which is especially suitable for reverse osmosis membrane pollution treatment in an industrial wastewater zero discharge system. The method comprises the following steps: regulating and controlling the composition of organic components of inlet water through a sand filtration pretreatment unit, and directly interfering the nucleation and crystallization processes of inorganic scaling on a membrane surface by utilizing naturally enriched microbial communities and extracellular polymeric substances thereof in a sand filtration process without adding a fungicide or a chemical scale inhibitor, so as to form a loose and reversible pollution layer structure. The method effectively reduces the scaling rate of inorganic salts such as calcium carbonate and the like, inhibits membrane surface biological pollution, delays compaction of a pollution layer, improves the membrane flux retention rate, prolongs the membrane operation cycle and reduces the cleaning frequency. Compared with an existing traditional physical or chemical pretreatment method, the natural scale inhibition effect of an ecological system in the sand filtration unit is fully utilized, and the method has the remarkable advantages of being low in operation cost, environmentally friendly, high in adaptability and the like.

The invention discloses a device and a method for low-carbon nitrogen and phosphorus removal of town sewage, which are mainly used for town sewage treatment, a continuous flow segmented water inlet three-section anoxic and aerobic alternating process is utilized, precipitated sludge enters a cyclone separator to be separated into heavy sludge and light sludge, the heavy sludge flows back to enter the first two sections of aerobic zones of a biochemical pool, and the light sludge flows back to the second two sections of aerobic zones of the biochemical pool. Part of the light sludge flows back into a first-section anoxic zone of the biochemical pool, and the residual light sludge is discharged as residual sludge; microorganisms in the light sludge store an internal carbon source in the anoxic zone and generate a large amount of extracellular polymeric substances, the polymeric substances promote the activated sludge to form granular sludge in the aerobic zone, and nitrification, endogenous short-cut denitrification-anaerobic ammonia oxidation nitrogen removal and phosphorus uptake are realized in the aerobic zone. According to the invention, low-carbon nitrogen and phosphorus removal of town sewage can be realized, the town sewage treatment efficiency can be effectively improved, the use of an external carbon source for sewage treatment is reduced, and the sewage treatment cost is reduced.

The invention belongs to the technical field of sewage treatment, and discloses a method for strengthening anaerobic fermentation of sludge and synchronously producing medium-chain fatty acid and hydrogen. The method comprises the following steps: settling and concentrating sludge, mixing the sludge with alkaline biochar, adding potassium ferrate for reaction to obtain pretreated sludge, adjusting the pH value, mixing the pretreated sludge with an electron donor and inoculated sludge, and carrying out anaerobic fermentation to obtain fermentation liquor rich in medium-chain fatty acid and hydrogen. An alkaline biochar-potassium ferrate coupling system is utilized to crack extracellular polymeric substances and cell walls of the sludge, so that particle organic matters are driven to be dissolved, and the activity of methane bacteria is inhibited; fe2O3 derived from potassium ferrate and biochar form an iron-carbon complex in situ, and a composite technology with triple functions of pretreatment, electronic regulation and methane inhibition is formed by strengthening electron transfer efficiency and enzyme activity and promoting an organic matter acidification process and a carbon chain extension reaction, so that synchronous recovery of medium-chain fatty acid and hydrogen is realized; the sludge treatment efficiency is improved.

The invention discloses an SRB remediation system capable of stably operating for treating heavy metal wastewater for a long time and application, belongs to the technical field of heavy metal wastewater treatment, and solves the problems that in the prior art, mining heavy metal wastewater generally contains high-concentration soluble heavy metal, high sulfate content and low pH value, the ecological environment is seriously damaged, and the service life of the mining heavy metal wastewater is prolonged. And the traditional physicochemical treatment method has the technical problems of high agent consumption, high sludge amount, easiness in secondary pollution and the like. According to the SRB remediation system capable of stably operating for a long time and used for treating heavy metal wastewater, efficient removal and immobilization of heavy metal are achieved on the basis of the biological vulcanization effect of SRB composite flora in a UASB bioreactor in combination with the synergistic effect of microbial communities and the protection mechanism of extracellular polymeric substances, and by constructing the composite SRB flora system, operation parameters are accurately regulated and controlled, so that the heavy metal wastewater treatment efficiency is improved. And efficient and stable removal and fixation of various heavy metals in the AMD are realized in the UASB reactor by utilizing a cooperative protection mechanism of EPS.

The invention belongs to the technical field of sewage treatment, and discloses a DO-ORP coordinated regulation artificial tidal food web sludge in-situ reduction and phosphorus recovery method. Aiming at the problems of high energy consumption, serious phosphorus resource loss and the like of traditional sludge treatment, a multi-stage food chain consisting of microorganisms, protozoa and metazoan is implanted by constructing an artificial tidal environment with periodic alternation of wetting and drying; according to the method, the metabolic activity of sludge hydrolysis flora and phosphorus-accumulating bacteria is activated by utilizing cooperative regulation and control of DO concentration and ORP, microbial extracellular polymeric substances are triggered to be depolymerized by adopting tidal periodic oscillation, in-situ reduction of sludge biomass is realized by combining a food web cascade predation effect, and phosphorus is synchronously removed by virtue of an anaerobic phosphorus release-aerobic phosphorus uptake coupled struvite crystallization process. The method is suitable for treating the excess sludge of a municipal sewage treatment plant, and the high-purity magnesium ammonium phosphate fertilizer is produced while the dehydration energy consumption is reduced by 40%.

The invention discloses a probiotic strain Bifidobacterium animalis subsp. Lactiss S8 of a high-yield biological membrane, the preservation number is GDMCC NO.66518, the characteristics of the stable high-yield biological membrane are obtained through directional screening and adaptive evolution, and the yield of the biological membrane is three times higher than that of a positive control. The extracellular polymeric substance (EPS) of the strain can significantly enhance the tolerance of the strain to severe environments such as gastric acid, cholate and the like, and improve the intestinal colonization ability. Experiments prove that the strain remarkably reduces two liver function indexes, namely ALT and AST, in an animal model, and shows that the strain has a certain effect of improving liver injury.

The present invention discloses a long-lasting intelligent composite antibacterial coating with dual pH response function and its preparation method, including: 1) constructing a nanotube structure on the surface of a pretreated titanium alloy substrate by a surface anodizing process; 2) combining a cyclic vacuum infusion + low-temperature micro-airflow process, infusing a povidone-iodine mixed solution into the nanotube pores on the titanium alloy surface; 3) after the titanium alloy surface is washed with water, a surface silanization treatment is performed to introduce a silane preparation with a double bond; 4) combining a surface cyclic light curing process, NAC-ChiMA is introduced into the substrate surface by a double bond addition reaction between the silane preparation and chitosan (NAC-ChiMA) modified with MA and NAC, to achieve the preparation of a composite antibacterial coating. The composite coating realizes the sequential release and slow release of NAC and povidone-iodine through the characteristics of vacuum drug loading and dual pH response, first destroying the extracellular polymeric substances of the bacterial biofilm, and then killing the bacteria in the biofilm, thereby achieving effective treatment of orthopedic implant infection.

The invention belongs to the field of microorganisms, and particularly discloses paenibacillus mucilaginosus JZ1 and application thereof. According to the invention, paenibacillus mucilaginosus with multifunctional characteristics is screened from biological crust. The paenibacillus mucilaginosus JZ1 strain obtained by the invention has multiple functions of dissolving phosphorus, dissolving potassium, fixing nitrogen, and producing IAA, ACC deaminase, siderophore and extracellular polymeric substance (ESP), is an environment-friendly biological material, and does not cause environmental pollution. The bacterial strain is combined with the crust algae for application, so that the effects of promoting plant growth and improving soil can be stably exerted. The method has a relatively great application prospect.

The application provides a sludge immobilization-based gel aerobic particle and a preparation method and application thereof, and belongs to the field of sewage treatment.The method comprises the following steps: 1, uniformly dispersing sEPS powder in a sodium alginate solution to obtain a composite gel; 2, filtering, centrifuging and removing supernatant of activated sludge in an aerobic tank to obtain concentrated bacteria solution; 3, adding the concentrated bacteria solution into the composite gel, uniformly mixing, dropping into a cross-linking solution, and then solidifying to obtain the gel aerobic particle.The sludge immobilization-based gel aerobic particle is prepared by using structural extracellular polymeric substances in activated sludge and sodium alginate to prepare a composite gel, and using a multivalent metal ion cross-linking agent to immobilize concentrated activated sludge, so that the gel aerobic particle with similar chemical composition, mechanical strength and biological performance to natural aerobic particles is prepared, and rapid granulation of sludge and rapid start-up operation of a granular system are realized.

The invention discloses a method for adsorbing and degrading high-concentration tetracycline in high-salinity wastewater by using activated sludge, which comprises the following two steps of: 1, directionally domesticating heterotrophic active sludge which takes sulfate reducing bacteria (SRB) as main functional bacteria based on a completely mixed reactor in a continuous water feeding manner; water-inflowing culture microorganisms with low-concentration tetracycline are added, so that the tolerance to tetracycline is improved, bacteria are promoted to secrete an extracellular polymeric substance (EPS), and the tetracycline is adsorbed by utilizing the EPS and is promoted to be degraded. By adjusting the pH value in stages, the horizontal transfer of antibiotic resistance genes is effectively inhibited. And 2, completing efficient degradation of high-concentration tetracycline by using the successfully domesticated anaerobic activated sludge. The method successfully realizes effective treatment of high-concentration tetracycline wastewater generated in industries such as mariculture and the like, inhibits diffusion of resistance genes, and has remarkable environmental benefits and economic benefits.

The invention discloses an efficient sludge dehydration conditioning method and device based on multi-pulse detonation shock waves, and relates to the technical field of sewage treatment, and the method comprises the steps of sludge pretreatment, multi-pulse detonation shock and energy recovery dehydration. Wherein the stepped pressure in the multi-pulse detonation impact is 0.5-4.0 MPa, and the pulse frequency is 1-10 Hz. The porous impact plate is arranged in the spherical detonation bin, the pulse electromagnetic valve is arranged on a pipeline, leading to the spherical detonation bin, of the high-pressure gas storage tank, and the porous impact plate is arranged at the outlet end of the pipeline. The energy recovery system comprises a residual pressure converter and a heat recovery pipe, the residual pressure converter pressurizes the mud cake, and the heat recovery pipe is used for heating the inlet sludge; the intelligent control system regulates and controls the pulse frequency and the pressure gradient through a PLC. According to the method, pulse type detonation shock waves with the controllable frequency of 1-10 Hz are adopted, a sludge extracellular polymeric substance (EPS) net structure is disintegrated layer by layer through the pressure accumulation effect, and energy waste caused by single-time impact is avoided.

This invention relates to the field of catalytic degradation technology for organic pollutants, and particularly to an iron-modified biochar immobilized catalase, its preparation method, and its application. The preparation method of the iron-modified biochar immobilized catalase includes the following steps: mixing iron-modified biochar, catalase solution, and an extracellular polymeric substance; performing an immobilization and cross-linking reaction; and separating the solid and liquid phases to obtain the iron-modified biochar immobilized catalase; the extracellular polymeric substance is secreted by *Goldenella*. This invention uses an extracellular polymeric substance instead of traditional glutaraldehyde as a cross-linking agent, avoiding the hazards associated with glutaraldehyde. Simultaneously, the prepared iron-modified biochar immobilized catalase exhibits higher enzyme activity and superior pollutant degradation performance.

The invention provides a method for forming a rhizopus arrhizus biological membrane, and aims to realize efficient and stable formation of the biological membrane by optimizing a culture medium formula and controlling culture conditions. The method comprises the following steps: preparing a culture medium with specific components, adding a carbon source, a nitrogen source, monopotassium phosphate, magnesium sulfate and various trace elements, and culturing by inoculating rhizopus arrhizus strains. In the culture process, the secretion of extracellular polymeric substances is promoted and the formation of biological membranes is accelerated by continuously supplying oxygen and adding a calcium chloride inducer. And culturing in a constant-temperature environment of 28-32 DEG C for 48-72 hours to obtain a stable rhizopus arrhizus biological membrane. Compared with the prior art, the culture method provided by the invention can effectively improve the thickness and stability of the biological membrane and shorten the formation period, and is suitable for industrial application requirements.

The invention relates to the technical field of sludge reduction, in particular to a mineral-based activated sludge conditioner and a sludge deep dehydration method. The activated sludge conditioner comprises PAC and modified mineral-PAM with positive charges on the surface, and when activated sludge is conditioned by the activated sludge conditioner, on one hand, the sedimentation and flocculation performance of the activated sludge can be improved through the combined effect of the modified mineral with the positive charges on the surface, PAC and sludge, and on the other hand, the sedimentation and flocculation performance of the activated sludge can be improved; on the other hand, the filtering property of the sludge can be improved, release of extracellular polymeric substances is facilitated, and the dehydration performance is improved. The method disclosed by the invention is simple and controllable in process flow, can effectively improve the sludge settling effect and the dewatering performance, and has a wide application prospect.

The invention discloses application of C8-HSL in promoting nitrogen and phosphorus removal capacity of an algal-bacterial symbiotic system in sewage treatment, the removal of ammonia nitrogen, TN and total phosphorus of the symbiotic system is improved by 25.17%, 36.22% and 38.74% by 0.8 mu M of C8-HSL, and meanwhile, the formation of extracellular polymeric substances (EPS) is increased by 68.62%. Metagenome sequencing results show that the Halomonas and the Sphingobium are main nitrogen and phosphorus removal microorganisms, the narGHI gene and the nirKS gene play a key role in the nitrogen removal process, and the phnX gene and the ppa gene play a key role in the phosphorus removal process. The research provides a theoretical basis and practical guidance for optimizing sewage treatment through a quorum sensing strategy.

The invention belongs to the technical field of biological energy and environmental engineering, and particularly discloses a method for treating a chlorella circulating culture solution through Fenton oxidative coupling activated carbon adsorption to solve the growth inhibition phenomenon caused by extracellular polymeric substance accumulation in the microalgae circulating culture process so as to achieve cyclic utilization of the chlorella culture solution and application of the chlorella culture solution. By means of Fenton oxidative coupling activated carbon adsorption, chlorella extracellular polymeric substances (EPS) can be effectively degraded and oxidized and decomposed into small-molecule organic substances, the growth inhibition effect of the small-molecule organic substances on chlorella is relieved, an organic carbon source can be provided for growth and metabolism of chlorella, and growth of chlorella is remarkably promoted. According to the method, the chlorella circulating wastewater is treated and recycled through Fenton oxidative coupling activated carbon adsorption, so that the yield of chlorella biomass can be effectively increased, and the production and culture cost is reduced.

The application discloses a kind of microbial flocculants and preparation method thereof, the preparation method includes the following steps: camellia cake meal is mixed with water, ultrasonic, hydrothermal treatment, filtration, filtrate is collected, and camellia cake meal extraction solution is obtained, configuration is camellia cake meal culture medium, inoculation flocculation function bacteria seed liquid is cultivated, and the preparation of microbial flocculants is completed.In the application, the way of ultrasonic extraction and hydrothermal extraction is combined, and camellia cake meal extraction solution with rich nutritional ingredients and good adaptability of various nutrients can be prepared, which can promote the rapid growth of flocculation function bacteria when used for culturing flocculation function bacteria, increase biomass, and induce more extracellular polymeric substance, thereby obtaining microbial flocculants with high yield and high flocculation rate.In the application, camellia cake meal is used as raw material, which can greatly reduce production cost and simplify production steps, promote large-scale use of microbial flocculants, and realize resource utilization of camellia cake meal.

The invention discloses a multistage enzyme and quorum sensing signal molecule MABR membrane and a preparation method thereof, through a bionic induction strategy, while the anti-pollution characteristic of the MABR membrane is maintained, multistage enzyme and quorum sensing signal molecules are fixed on the surface of the membrane, and the multistage enzyme and quorum sensing signal molecules induce microorganisms to secrete an extracellular polymeric substance (EPS), so that the binding strength of a biological membrane is improved by 40% or more. The preparation method of the multi-stage enzyme and quorum sensing signal molecule MABR membrane comprises the following steps: (1) screening nitrifying bacteria and extracting multi-stage enzyme and quorum sensing signal molecules of the nitrifying bacteria; (2) carrying out composite pretreatment on the surface of the MABR membrane, wherein the composite pretreatment comprises hydrophilic modification, functional group introduction, swelling of the surface of membrane silk and the like; and (3) immobilizing multistage enzyme and quorum sensing signal molecules (including cell membrane fragments, periplasmic space enzyme complexes and the like) in the strain by adopting an immobilization technology to form a bioactive interface layer with the thickness of about 50-200nm.

The application belongs to the technical field of microbial sewage treatment, and particularly relates to an iron-carbon quantum dot / electrically active non-oxygen-producing photosynthetic bacteria / carrier hybrid cell factory and application thereof in sewage resourceization or carbon emission reduction; the iron-carbon quantum dot in the hybrid cell factory provided by the application promotes the non-oxygen-producing purple photosynthetic bacteria to utilize more light energy, and generates photo-generated electrons as an extracellular efficient electron donor source, and the photosensitive active substances in the extracellular polymeric substance of the non-oxygen-producing purple photosynthetic bacteria can promote the formation of a continuous cycle of trivalent iron and divalent iron, realize mutual cooperation, promote sewage resourceization and carbon emission reduction, and synthesize high-value-added products; and in addition to facilitating the recovery of biomass, the transparent conductive glass electrode as the carrier also ensures that the non-oxygen-producing purple photosynthetic bacteria stably and efficiently utilize light energy; therefore, the hybrid cell factory provided by the application is a high-efficiency microbial sewage treatment technology, and solves the technical problem of lack of high-efficiency microbial sewage treatment technology in the prior art.

The invention belongs to the technical field of sludge treatment, and provides a method for strengthening anaerobic fermentation of sludge by activated sodium pyrosulfite, which comprises the following steps: pretreating sludge to obtain a sludge system; adding ferric salt and sodium pyrosulfite into the sludge system to obtain an activation system; and performing anaerobic fermentation on the active system to finish the treatment. By constructing a Fe < 3 + > / sodium pyrosulfite free radical system, the dissolution of organic matters in the sludge is improved, so that the generation of short-chain fatty acid (SCFAs) in the anaerobic fermentation process is promoted. Sodium pyrosulfite is dissolved in water to form sulfite species, under the action of ferric ions, the sulfite species can generate various active free radicals through electron transfer, and the free radicals have high oxidation capacity and can destroy sludge floc structures and extracellular polymeric substances, so that cell lysis is promoted, intracellular organic substances are released, and the sludge can be degraded. The concentration of a substrate available for anaerobic fermentation is improved, and the yield of short-chain acid is substantially improved.

The present application belongs to the field of sewage biological treatment technology, and particularly relates to a method for extracting different extracellular polymer components from activated sludge. The method focuses on layered extraction of various components of different extracellular polymers, that is, S-EPS in the activated sludge is first extracted by centrifugation, then LB-EPS is extracted by ultrasonic method and high-speed centrifugation, ion TB-EPS is extracted by EDTA method and high-speed centrifugation, and finally, hydrophobic TB-EPS is extracted by ethanol method and high-speed centrifugation. The combination of the above methods can complete the extraction of different components of extracellular polymers. The method for extracting different extracellular polymer components from activated sludge provided by the present application can ensure high extraction efficiency and high purity of EPS, and at the same time, the influence of the extraction method on cell lysis is as small as possible, and the quantitative accuracy and feasibility of the extracellular polymer extraction method are ensured.

The application belongs to the technical field of sewage treatment, and specifically discloses a sludge deep dewatering conditioner based on tannin acid grafting polymer and a synthesis method and industrial application thereof. The conditioner is a novel derivative of tannin acid grafted on a synthetic polymer or natural polymer main chain by chemical bonding, which contains high-density cationic active sites and three-dimensional network chelating groups in the molecular structure, and has strong electric neutralization capacity and multi-level adsorption characteristics. The derivative is realized by directional synthesis technology for the first time and is innovatively applied in the field of sludge dewatering, can efficiently destroy the structure of sludge extracellular polymeric substances, and significantly improve the combined water release efficiency. The conditioner is precisely designed in the molecular structure, can reduce the dosage of the conditioner while realizing more than 20% increase of the sludge dewatering rate, and the grafting rate and charge density parameters constitute the core protection features, thereby breaking through the technical bottleneck of traditional flocculants in sludge deep dewatering.

The invention discloses a method for improving the anaerobic corrosion hydrogen evolution effect of nanoscale zero-valent iron. The nanoscale zero-valent iron is coated with any one of sodium carboxymethyl cellulose, extracellular polymeric substances and lauryl sodium sulfate so as to improve the corrosion hydrogen evolution performance of the nanoscale zero-valent iron. According to the method, polymer precursors such as CMC, EPS and SDS are added, and the nano zero-valent iron is modified in an anaerobic environment, so that the corrosion hydrogen evolution effect of the nano zero-valent iron is improved. Under the condition that the addition amounts of CMC, EPS and SDS are 400 mg / L, 10 mg / L and 12 mg / L respectively, the polymer not only can form a protective layer on the surface of the nano zero-valent iron, but also can enhance the hydrogen evolution effect of the nano zero-valent iron, and the accumulated hydrogen production amounts of the nano zero-valent iron modified by the polymer within 14 days are 1042 mL, 1066 mL and 1210 mL respectively and are increased by 6.11%-10.30% compared with a blank group.

This invention discloses a method for extracting extracellular polymers from benthic microalgae culture medium, as well as the products and applications thereof, comprising the following steps: adding formamide to the benthic microalgae culture medium, adding Na... + The extracellular polymeric substance (ECPL) is obtained by stirring, centrifuging, heating and concentrating, freeze-drying, dissolving in water, adding anhydrous ethanol, precipitating, dissolving again in water, dialyzing, and freeze-drying. This invention improves upon existing ethanol precipitation processes by employing an extraction process of "heating and concentrating benthic microalgae culture medium—freeze-drying to obtain a crude extract—redissolving the crude extract in water—precipitating with anhydrous ethanol—dissolving the precipitate—dialysis—freeze-drying to obtain extracellular polymeric substances." Compared to traditional extraction processes, this method allows for "controllable" ethanol dosage by dissolving a large amount of crude extract in a small amount of water to obtain a solution rich in extracellular polymeric substances. Furthermore, it significantly reduces the amount of anhydrous ethanol used when extracting large quantities of extracellular polymeric substances, greatly lowering experimental costs.