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Aerobiotic heterotrophic bacteria extracellular polymeric substance for removing stress of heavy metals and preparation method

A technology of extracellular polymers and heavy metals, applied in chemical instruments and methods, water pollutants, biological water/sewage treatment, etc., can solve the problem of weak removal capacity of heavy metals, and achieve the effect of simple operation

Pending Publication Date: 2016-08-24
GUANGDONG UNIV OF TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, the extracellular polymers directly extracted from the bacteria have weak ability to remove heavy metals

Method used

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  • Aerobiotic heterotrophic bacteria extracellular polymeric substance for removing stress of heavy metals and preparation method
  • Aerobiotic heterotrophic bacteria extracellular polymeric substance for removing stress of heavy metals and preparation method

Examples

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Embodiment 1

[0056] Example 1 Preparation of Extracellular Polymer

[0057] In this example, Bacillus vallismortis sp. (Bacillus vallismortis sp., belonging to aerobic heterotrophic bacteria, obtained by separation and purification in a laboratory) was selected. The specific preparation steps of the extracellular polymer are as follows:

[0058] The Bacillus dead valley stored at -20°C was taken out, thawed, activated and enriched to make the Bacillus dead valley suspension. Inoculate the bacterium suspension into 5 Erlenmeyer flasks containing 100mL liquid culture medium (the liquid culture medium was autoclaved at 121°C for 30min) respectively according to the inoculation amount of 5%, and inject a certain volume of sterilized Cu 2+ Mother liquor, make Cu in 5 Erlenmeyer flasks 2+ Concentrations were 0, 5, 10, 15, 20mg / L, and the Cu 2+ Stress culture for 24h. The above different Cu 2+ The Bacillus dead valley culture after the concentration stress culture is subjected to the first ...

Embodiment 2

[0063] Example 2 Preparation of Extracellular Polymer

[0064] In this embodiment, Pseudomonas aeruginosa (P. aeruginosa, belonging to aerobic heterotrophic bacteria, purchased from the market) was selected. The specific preparation steps of the extracellular polymer are as follows:

[0065] The Pseudomonas aeruginosa stored at -20°C was taken out, thawed, activated and enriched to make a Pseudomonas aeruginosa suspension. Inoculate the bacterial suspension into 5 Erlenmeyer flasks containing 100mL liquid culture medium (the liquid culture medium was autoclaved at 121°C for 30min) according to the inoculation amount of 5%. After culturing for 4 hours, inject a certain volume into the liquid medium at the same time The sterilized zinc ion mother solution made the zinc ion concentration in 5 Erlenmeyer flasks respectively 0, 5, 10, 15, 20mg / L, and carried out the zinc ion stress culture under the conditions of 35°C and 150rpm for 20h. Perform the first centrifugation on the Ps...

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Abstract

The invention relates to the technical fields of bioengineering and wastewater treatment and particularly relates to an extracellular polymeric substance, a heavy metal treating agent and a heavy metal treating method. A preparation method of the extracellular polymeric substance comprises the steps of carrying out heavy metal stress treatment on aerobiotic heterotrophic bacteria when the aerobiotic heterotrophic bacteria are cultured, and removing a culture medium, heavy metals and thalluses, so as to obtain the extracellular polymeric substance. The extracellular polymeric substance provided by the invention has outstanding capacity for removing specific heavy metals and can be applied to the treatment of heavy metal wastewater.

Description

technical field [0001] The invention relates to the technical fields of bioengineering technology and wastewater treatment, in particular to an extracellular polymer, a heavy metal treatment agent and a heavy metal treatment method. Background technique [0002] Heavy metal pollution refers to environmental pollution caused by heavy metals or their compounds, mainly caused by human factors such as mining, waste gas discharge, sewage irrigation, and the use of products with heavy metals exceeding the standard. Heavy metals refer to metals with a specific gravity greater than 5, including gold, silver, copper, iron, lead, etc. Heavy metals can strongly interact with proteins and various enzymes in the human body, making them inactive, and may also accumulate in certain organs of the human body. If the human body exceeds the tolerance limit, it will cause acute poisoning, Subacute poisoning, chronic poisoning, etc., will cause great harm to the human body. For example, public ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/00C12P19/04C12P19/34C02F3/34C02F101/20
CPCC02F3/34C02F2101/20C12P19/04C12P19/34C12P21/00
Inventor 宋卫锋简静仪李海宇董明杨梓亨
Owner GUANGDONG UNIV OF TECH
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