Biological membrane extracellular polymeric substance extraction and grading analysis method of biological membrane reactor

Abstract

The invention discloses a biological membrane extracellular polymeric substance extraction and grading analysis method of a biological membrane reactor. The biological membrane extracellular polymeric substance extraction and grading analysis method comprises the following steps of: extracting biological membrane extracellular polymeric substance mucus, loose extracellular polymeric substance and tight type extracellular polymeric substance; respectively carrying out chromatography grading on the biological membrane extracellular polymeric substance mucus, loose extracellular polymeric substance and tight type extracellular polymeric substance by adopting XAD-8 adsorption resin, MSC cation exchange resin and Duolite A-7 anion exchange resin; and carrying out system analysis on each graded component of the biological membrane extracellular polymeric substance mucus, loose extracellular polymeric substance and tight type extracellular polymeric substance. By adopting the biological membrane extracellular polymeric substance extraction and grading analysis method, the problems that the original biological membrane structural characteristics are destroyed in general biological membrane stripping, synchronous separation of biological membrane extracellular polymeric substance and hydrophilic, hydrophobic and acid and alkaline substances is difficult and characteristic analysis is not systematic can be solved.

Description

technical field [0001] The invention relates to the field of extraction and analysis of extracellular polymers of biofilms, in particular to a method for extraction and fractional analysis of extracellular polymers of biofilms in biofilm reactors. Background technique [0002] Extracellular polymers (EPS) are produced by microorganisms during their metabolism under certain environmental conditions and are enclosed outside the cell wall for self-protection and mutual adhesion, and provide carbon sources and energy for the microorganisms themselves in starvation environments. A class of organic polymers. In the process of biological wastewater treatment, EPS includes not only mucus and capsules secreted by cells, but also microbial excreta, metabolic and hydrolyzed products, and some inorganic and organic substances in the adsorbed wastewater. In the process of treating wastewater and remediating polluted water bodies in biofilm reactors, EPS is considered to play a very impo...

AI Technical Summary

Problems solved by technology

This method is used to solve the problem that biofilm exfoliation usually destroys the original biofilm structural characteristics, and solves the problem that after conventional biofilm exfoliation affects the biofilm extracellular polymer slime (S), loose extracellular polymer (LB) and compact extracellular polymer. The shortcomings of polymer (TB) extraction can also solve the problems of synchronous separation of hydrophilic, hydrophobic, acid-base substances and unsystematic feature analysis of biofilm extracellular polymers

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