48results about "Anion exchanger materials" patented technology

The present application relates to the technical field of ion detection, in particular to a high-efficiency anti-interference ion chromatography detection device and method, through sample pretreatment module, the original sample is filtered, enriched and interference shielding processing, effectively removes impurities and shields interfering ions, at the same time, the chromatographic separation module realizes the simultaneous separation of anions and cations under constant temperature conditions, avoids the problems of chromatographic peak overlap and tailing under the traditional single separation mechanism, significantly improves the detection stability and accuracy of complex matrix samples; the eluent supply module generates high-purity eluent online to avoid impurity pollution caused by manual preparation, the detection module reduces the background conductance in the ion flow and converts the ion concentration change into an electrical signal, effectively reduces the baseline noise, meets the high-sensitivity detection requirement of trace ions; the central control unit controls the cooperative work of each module, greatly shortens the analysis time, and is suitable for rapid detection of large quantities of samples.

The present invention pertains to the purification of soluble complement receptor proteins and variants thereof by hydrophobic interaction chromatography (HIC) using a chromatographic material with a particle size of less than 60 µm.

The present disclosure relates to a preparation method for L-citrulline, comprising: Step 1 of preparing a fermentation broth comprising L-citrulline; Step 2 of performing a continuous chromatography process on the fermentation broth to obtain a purified citrulline process liquid; and Step 3 of crystallizing and separating L-citrulline in the purified citrulline process liquid. According to the present disclosure, high purity L-citrulline crystals can be obtained in high yield from the fermentation broth.

The application discloses a preparation and purification method of high-purity radioiodine-labeled antigen. The method comprises the following steps: pre-activating carrier-free sodium iodine[125I] solution in an orthogonal solvation sensitization buffer containing lithium sulfate, 18-crown-6 and beta-cyclodextrin; adding antigen and chloramine-T for a labeling reaction; and performing magnetic separation and purification by using a suspension containing adamantane-modified magnetic microspheres and quaternary ammonium salt-modified strong anion exchange magnetic microspheres after the reaction. The orthogonal solvation effect is used to enhance the nucleophilic activity of iodine ions, and the amount of oxidizing agent is reduced to reduce protein damage; impurities are quickly removed through the double mechanisms of electrostatic adsorption and host-guest recognition. The application has the advantages of high labeling efficiency, high radiochemical purity of the product and complete retention of biological activity.

This invention belongs to the field of targeted α-nucleonite separation and extraction technology, specifically disclosing a method for separating and extracting lead-212 and bismuth-212 using anion exchange fibers. An acidic thorium solution is passed through an anion exchange fiber column, utilizing the fact that halogen anions can react with Pb. 2+ / Bi 2+ The formation of complex anions is selectively adsorbed by anion exchange fibers. A leaching agent is then introduced to remove other impurity ions. Finally, a desorption agent is introduced to desorb lead-212 and bismuth-212, thus achieving the separation and extraction of lead-212 and bismuth-212 from the natural thorium decay chain. This invention utilizes the above-described method for separating and extracting lead-212 and bismuth-212 using anion exchange fibers. This method eliminates the need for accelerators or reactors, and offers advantages such as simple equipment and the ability to simultaneously handle radioactive waste treatment and disposal while also enabling the separation and extraction of high-value-added targeted alpha nuclides.

Provided are methods for isolating recombinant adeno-associated virus (AAV)-based vectors using anion exchange chromatography.

The application relates to the technical field of agricultural product quality and safety detection, and discloses a pesticide residue micro-extraction pretreatment device for agricultural products, which comprises a base, a sliding column is fixedly installed at the top of the base, a control driving assembly, a chopping and stirring assembly, a centrifugal assembly and a filtering assembly are arranged outside the sliding column, a fixing disc is fixedly installed outside the sliding column, a driving box is fixedly installed at the top of the fixing disc, and two driving gears and one transmission gear are rotationally connected in the driving box. The pesticide residue micro-extraction pretreatment device for agricultural products and the detection method integrate the chopping and stirring assembly, the control driving assembly, the centrifugal assembly and the filtering assembly into one through the single vertical main body structure formed by the base and the sliding column, a single servo motor synchronously drives the core working procedure through the transmission gear and the driving gear, there is no separate independent equipment, and the whole machine is compact and can be integrally moved.

The invention relates to a method for selective separation of amphoteric species, in particular biomolecules or biomolecule complexes, e.g., vectors containing molecule, based on their isoelectric points. For selective separation, a charged porous surface is provided being electrically conductive and / or comprising an electrically conductive coating or layer. Fluid containing the amphoteric species is brought in contact with the surface, thereby absorbing species to the surface due to an attractive interaction force. Thereafter, at least parts of the adsorbed species are released by applying a release voltage chosen so that the net interaction force between the surface and adsorbed species is switched from attractive to repulsive. Selectivity is achieved by selective adsorption, thus at least one species being not absorbed, and / or selective desorption, thus selectively releasing some species previously adsorbed with others remaining bound to the surface, preferably selectively releasing solely one species of the previously adsorbed.

An extraction module includes an axial chamber positioned about a central axis; and a plurality of extraction leaves arranged in a stack. Each extraction leaf extends outward from the central axis in a radial curvature and includes a porous active layer, a first nonporous backing layer, a first porous spacer layer disposed between the porous active layer and the first nonporous backing layer, a second porous spacer layer disposed on the porous active layer on a side opposite the first porous spacer layer, and optionally, a second nonporous backing layer disposed on the second porous spacer layer on a side opposite the porous active layer. The extraction module can be particularly useful for extraction of a target species from a fluid.

A method of making 1,5 pentanediamine (a.k.a. PDA, pentamethyldiamine, PMDA and cadaverine) by decarboxylation of lysine using lysine decarboxylase in the presence of sulfate, or more preferably phosphate anion salts of lysine is described. The process works at high pHs at which the decarboxylase does not function using HCL salts of lysine and at substrate concentrations not achievable with lysine HCL at the ordinary pH optima of the carboxylase. In addition, a chromatographic process for purifying the PDA from lysine and the conjugate anions of the lysine salt is described that is applicable as a general process for purifying any diamine from a product mixture comprising amino and / or carboxylic acids along with their conjugate anions, which method uses a strong anion exchange resin as the stationary phase. The method does not utilize any organic solvent and is shown to be economical and efficient for industrial scale isolation of diamines from complex mixtures containing amino and / or carboxylic acids and conjugate anions from the amine salts.

Provided herein are methods for purifying recombinant adeno-associated virus (rAAV) particles using chromatography in a large scale. Further provided include the rAAV particles and the pharmaceutical composition prepared by the method.

The present invention relates to methods of reducing the rate of enzymatic hydrolysis activity in compositions obtained from a purification platform, and provides purification platforms comprising a depth filtration step and / or a hydrophobic interaction chromatography (HIC) step. The present invention also discloses methods of using the purification platforms described herein and compositions, such as pharmaceutical compositions, obtained from the purification platforms.

The present invention relates to methods for polishing and enrichment of full adeno-associated virus particles by anion exchange chromatography using basic amino acids in the elution buffer.

The invention provides a chromatographic method for separating pre-contraction state PVC Nanosyringes from post-contraction state PVC Nanosyringes, the method comprising: (a) providing a sample containing both pre- and post-contraction state PVC Nanosyringes; (b) loading the sample on a monolith anion exchange column having (preferably quaternary amine) binding moieties, under conditions (e.g. pH 7-8, temperature 19-25°C) wherein pre- and post-contraction state PVC Nanosyringes become bound to the column with differing affinities; (c) applying a gradient of ionic strength to the column that provides for differential elution of the pre- and post-contraction state PVC Nanosyringes, wherein the gradient is generated by incrementally (e.g. linearly) increasing the salt concentration of a mobile phase elution buffer that is applied to the column; and (d) eluting the pre- and post-contraction state PVC Nanosyringes into separate elution fractions under the gradient based on their differing affinities to the anion exchange column, and collecting fractions having the pre-contraction state PVC Nanosyringes separated from the post-contraction state PVC Nanosyringes.

The present disclosure provides processes for relates to processes for separating one or more water-soluble products from non-woody plants of the genus Hesperaloe. The processes described herein sequentially separates one or more water-soluble products from non-woody plants of the genus Hesperaloe. The process may include sequential separation and enrichment of different saponins, polyphenols, and / or organic acids from a juice derived from non-woody plants of the genus Hesperaloe. The sequential separation of water-soluble products from non-woody plants of the genus Hesperaloe provides multiple high-value products.

The application provides an ion exchange resin and a preparation method thereof. The preparation method of the ion exchange resin comprises the following steps: performing a polymerization reaction on a mixed system comprising a support material, an acrylate monomer, a crosslinking agent and an initiator to obtain a composite microsphere; performing a chloromethylation reaction on the composite microsphere, a second solvent and a chloromethylation reagent to obtain a composite chloromicrosphere; and performing an amination reaction or a sulfonation reaction on the composite chloromicrosphere to obtain the ion exchange resin. The ion exchange resin prepared by the preparation method has the advantages of high mechanical strength and strong adsorption capacity. When the ion exchange resin is applied to the purification of a crude terephthalic acid diethylene glycol ester, the ion exchange resin can be stably operated in a water system, the generation of a hydrolysis byproduct is reduced, the product purity is significantly improved, and the industrial purification of waste polyester alcoholysis products is effectively supported.

To provide methods of reducing bioburden of a chromatography resin.SOLUTION: Provided herein are methods of reducing bioburden of a chromatography resin that include exposing a container including a composition including (i) a chromatography resin and (ii) a liquid including at least one alcohol to a dose of gamma-irradiation sufficient to reduce the bioburden of the container and the chromatography resin, where the at least one alcohol is present in an amount sufficient to ameliorate the loss of binding capacity of the chromatography resin after / upon exposure to the dose of gamma-irradiation. Also provided are reduced bioburden chromatography columns including the reduced bioburden chromatography resin, compositions including a chromatography resin and a liquid including at least one alcohol, and methods of performing reduced bioburden column chromatography using one of these reduced bioburden chromatography columns.SELECTED DRAWING: Figure 1

The application belongs to the technical field of lithium ion battery electrolyte, and relates to a method and system for continuously preparing high-purity liquid bisfluorosulfonylimide lithium. The application generates molten LiFSI through one-pot reaction of bischlorosulfonylimide, anhydrous hydrogen fluoride and lithium fluoride in a micro-reactor at 80-150 DEG C and 0.5-3.0 MPa; the molten salt is dropped into low-temperature dichloromethane for back extraction and crystallization; the crude product is obtained through continuous filtration, washing and drying; the crude product is dissolved in an ester solvent, and then sequentially subjected to continuous membrane filtration, resin deacidification and deep water removal to obtain high-purity liquid LiFSI product. The method realizes one-pot fluorination, salt formation and full-process continuous production from raw materials to liquid product, has the advantages of high safety, high efficiency, high product purity, stable quality and convenience for large-scale industrial production. In the prepared high-purity LiFSI liquid salt, the water content is less than 10 ppm, the free acid (calculated by HF) is less than 20 ppm, the Cl ‑ 5 ppm, the SO4 2‑ 5 ppm, and the single content of various metal impurity ions (such as calcium, iron, magnesium, sodium and potassium) is less than or equal to 2 ppm.

The present disclosure provides, in some embodiments, devices, methods, and kits for purifying extracellular vesicles (EVs) using size exclusion chromatography in tandem with cation exchange chromatography, which can be referred to as dual-mode chromatography (DMC).

Provided is a complex composition, of which positional isomers are minimized by using a N-terminus of an immunoglobulin Fc region as a binding site when the immunoglobulin Fc region is used as a carrier. Also provided are a protein complex which is prepared by N-terminal-specific binding of immunoglobulin Fc region, thereby prolonging blood half-life of the physiologically active polypeptide, maintaining in vivo potency at a high level, and having no risk of immune responses, a preparation method thereof, and a pharmaceutical composition including the same for improving in vivo duration and stability of the physiologically active polypeptide. The protein complex may be usefully applied to the development of long-acting formulations of various physiologically active polypeptide drugs.

The utility model relates to the technical field of Kathon production, in particular to a mixing system for Kathon production, which comprises a first dissolving kettle and a second dissolving kettle, a discharge port of the first dissolving kettle is communicated with a first impurity removal device, a discharge port of the second dissolving kettle is communicated with a second impurity removal device, a discharge port of the first impurity removal device is communicated with a first buffer kettle, and a discharge port of the second buffer kettle is communicated with a second buffer kettle. A discharge port of the second impurity removal device is communicated with a second buffer kettle, a discharge port of the first buffer kettle and a discharge port of the second buffer kettle are respectively communicated with a mixing kettle, and a discharge port of the mixing kettle is communicated with a product storage tank. The mixing system with the structure is reasonable in design, and the purity and the stability of Kathon products are improved.

The present invention relates to a method for preparing a plasma sample comprising amyloid beta peptides for analysis by mass spectrometry, comprising the steps of:a) contacting said plasma sample with a denaturing agent, b) performing a first solid phase extraction step on the solution obtained in step a) to recover a first eluate, c) performing a second solid phase extraction step on said first eluate obtained in step b) to recover a second eluate, and d) drying said second eluate obtained in step c) and processing it for analysis by mass spectrometry, wherein the solution obtained in step d) comprises intact amyloid beta peptides Aβ40 and Aβ42.

This invention provides a method for separating and purifying uronic acid polysaccharides, belonging to the field of polysaccharide separation and purification technology. This invention utilizes the electrostatic adsorption of sodium ions in an aqueous sodium chloride solution around the carboxyl anions of uronic acid units, forming a charge shielding layer. Chloride ions in the aqueous sodium chloride solution are also adsorbed around the dextran gel via electrostatic interaction, effectively shielding the electrostatic attraction between the uronic acid units and the dextran gel, reducing the non-specific adsorption of uronic acid units and the dextran gel, making the uronic acid polysaccharides easy to separate and with high purity. Furthermore, sodium and chloride ions in the aqueous sodium chloride solution are strongly hydrated ions, preferentially binding to the hydroxyl groups on the dextran gel and the carboxyl groups on the uronic acid units, occupying hydrogen bond sites, disrupting the existing hydrogen bonds between the uronic acid units and the dextran gel, preventing the formation of new hydrogen bonds, reducing the adsorption of uronic acid units in the dextran gel, making the uronic acid polysaccharides easy to separate and with high purity.

The present invention provides methods of reducing yellow color in an Fc-containing molecule sample, such as in an elution pool. Said methods are useful in manufacturing of therapeutic antibodies.

Described are methods for producing recombinant Arginase, such as PEGylated, cobalt-substituted recombinant human Arginase 1. Also described are pharmaceutical compositions comprising such recombinant Arginase, as well as methods of treatment and uses of such recombinant Arginase.

The present invention relates to a pretreatment method for isolating a TGF-β3 protein from E. coli and purifying same with high efficiency and high purity, and a TGF-β3 protein purification method comprising the pretreatment method. The pretreatment method and the purification method comprising same, according to the present invention, use E. coli, and thus are expected to have simpler processes and higher productivity than animal cell culture methods such that highly-pure TGF-β3 protein can be isolated from E. coli and purified with high efficiency and high purity.

The presently claimed and described technology provides a chromatography method for purifying ribonucleic acid (RNA) using an elution buffer with a pH of 10 at least about and comprising at least about 0.80 M of at least one salt and a neutralizing solution.

The application discloses a kind of oil foam characteristic abnormality regeneration treatment device and treatment method for electric power equipment, device includes treatment device ontology and the regeneration treatment filter material being set in it, and regeneration treatment filter material is combined by special wood fiber adsorption filter piece and modified ion exchange resin;Treatment device ontology includes filter cartridge and the oil inlet hole and oil outlet hole being set on it.Special wood fiber adsorption filter piece is made of wood fiber by degreasing, composite activated carbon, forming punching and surface modification, and composite defoaming agent is sprayed;Modified ion exchange resin is prepared by polystyrene skeleton multi-step modification.This application is alternately stacked by filter piece and resin, so that oil liquid is subjected to physical filtration and chemical adsorption in device multiple times, and a variety of degradation products such as submicron paint film, surfactant, acidic substance and metal ion are removed simultaneously, to realize the overall recovery of foam characteristics and air release value, with the characteristics of high efficiency, wide coverage and no secondary pollution.

This invention discloses a method and system for real-time pH control and stabilization in online column separation, belonging to the field of ion exchange chromatography separation technology. The method includes: applying a proton perturbation signal to the chromatographic column and detecting its elution response signal to obtain a parameter spectrum characterizing the dynamic buffer capacity of the chromatographic column; inputting the parameter spectrum and the target pH gradient into a physical transport model based on convection, diffusion, and adsorption coupling equations; the physical transport model outputs a pre-distorted pH gradient program to compensate for the dynamic buffer capacity characteristics; and based on the fluid ratio requirements defined by the pre-distorted pH gradient program and varying over time. This allows for the acquisition of dynamic buffer capacity characteristics through proton perturbation testing and the construction of a pre-distorted pH gradient program. Combined with closed-loop iterative optimization, this effectively counteracts the interference of the chromatographic column's dynamic buffer capacity on proton transport, ensuring that the pH curve of the post-column elution accurately matches the target linear gradient and eliminating pH nonlinear distortion and plateau problems.

The invention relates to a process for producing medical-grade lead-212, comprising the steps of: a) production of lead-212 by radioactive decay of radium-224 in at least one first chromatography column containing a first stationary phase on which radium-224 is fixed; b) elution of the lead-212 thus produced from the first stationary phase; c) loading of the eluate thus obtained into a second chromatography column containing a second stationary phase to fix the lead-212 on the second stationary phase; d) washing of the second stationary phase; d) elution of the lead-212 from the second stationary phase; and characterized in that: - step b) comprises circulating in said at least one first chromatography column an ​​aqueous solution A1 comprising from 0.8 mol / L to 1.6 mol / L of a chloride, optionally with at most 200 mmol / L of HCl;and- step d) includes circulating an aqueous solution A2 comprising 0.01 mol / L to 1 mol / L of chloride through the second chromatography column. Applications: manufacture of lead-212 based radiopharmaceuticals, useful in nuclear medicine.