61results about "Organic cation exchangers" patented technology

The present application relates to the technical field of ion detection, in particular to a high-efficiency anti-interference ion chromatography detection device and method, through sample pretreatment module, the original sample is filtered, enriched and interference shielding processing, effectively removes impurities and shields interfering ions, at the same time, the chromatographic separation module realizes the simultaneous separation of anions and cations under constant temperature conditions, avoids the problems of chromatographic peak overlap and tailing under the traditional single separation mechanism, significantly improves the detection stability and accuracy of complex matrix samples; the eluent supply module generates high-purity eluent online to avoid impurity pollution caused by manual preparation, the detection module reduces the background conductance in the ion flow and converts the ion concentration change into an electrical signal, effectively reduces the baseline noise, meets the high-sensitivity detection requirement of trace ions; the central control unit controls the cooperative work of each module, greatly shortens the analysis time, and is suitable for rapid detection of large quantities of samples.

The present invention pertains to the purification of soluble complement receptor proteins and variants thereof by hydrophobic interaction chromatography (HIC) using a chromatographic material with a particle size of less than 60 µm.

The present disclosure relates to a preparation method for L-citrulline, comprising: Step 1 of preparing a fermentation broth comprising L-citrulline; Step 2 of performing a continuous chromatography process on the fermentation broth to obtain a purified citrulline process liquid; and Step 3 of crystallizing and separating L-citrulline in the purified citrulline process liquid. According to the present disclosure, high purity L-citrulline crystals can be obtained in high yield from the fermentation broth.

This invention belongs to the field of targeted α-nucleonite separation and extraction technology, specifically disclosing a method for separating and extracting lead-212 and bismuth-212 using anion exchange fibers. An acidic thorium solution is passed through an anion exchange fiber column, utilizing the fact that halogen anions can react with Pb. 2+ / Bi 2+ The formation of complex anions is selectively adsorbed by anion exchange fibers. A leaching agent is then introduced to remove other impurity ions. Finally, a desorption agent is introduced to desorb lead-212 and bismuth-212, thus achieving the separation and extraction of lead-212 and bismuth-212 from the natural thorium decay chain. This invention utilizes the above-described method for separating and extracting lead-212 and bismuth-212 using anion exchange fibers. This method eliminates the need for accelerators or reactors, and offers advantages such as simple equipment and the ability to simultaneously handle radioactive waste treatment and disposal while also enabling the separation and extraction of high-value-added targeted alpha nuclides.

Provided are methods for isolating recombinant adeno-associated virus (AAV)-based vectors using anion exchange chromatography.

The application relates to the technical field of agricultural product quality and safety detection, and discloses a pesticide residue micro-extraction pretreatment device for agricultural products, which comprises a base, a sliding column is fixedly installed at the top of the base, a control driving assembly, a chopping and stirring assembly, a centrifugal assembly and a filtering assembly are arranged outside the sliding column, a fixing disc is fixedly installed outside the sliding column, a driving box is fixedly installed at the top of the fixing disc, and two driving gears and one transmission gear are rotationally connected in the driving box. The pesticide residue micro-extraction pretreatment device for agricultural products and the detection method integrate the chopping and stirring assembly, the control driving assembly, the centrifugal assembly and the filtering assembly into one through the single vertical main body structure formed by the base and the sliding column, a single servo motor synchronously drives the core working procedure through the transmission gear and the driving gear, there is no separate independent equipment, and the whole machine is compact and can be integrally moved.

The invention relates to a method for selective separation of amphoteric species, in particular biomolecules or biomolecule complexes, e.g., vectors containing molecule, based on their isoelectric points. For selective separation, a charged porous surface is provided being electrically conductive and / or comprising an electrically conductive coating or layer. Fluid containing the amphoteric species is brought in contact with the surface, thereby absorbing species to the surface due to an attractive interaction force. Thereafter, at least parts of the adsorbed species are released by applying a release voltage chosen so that the net interaction force between the surface and adsorbed species is switched from attractive to repulsive. Selectivity is achieved by selective adsorption, thus at least one species being not absorbed, and / or selective desorption, thus selectively releasing some species previously adsorbed with others remaining bound to the surface, preferably selectively releasing solely one species of the previously adsorbed.

An extraction module includes an axial chamber positioned about a central axis; and a plurality of extraction leaves arranged in a stack. Each extraction leaf extends outward from the central axis in a radial curvature and includes a porous active layer, a first nonporous backing layer, a first porous spacer layer disposed between the porous active layer and the first nonporous backing layer, a second porous spacer layer disposed on the porous active layer on a side opposite the first porous spacer layer, and optionally, a second nonporous backing layer disposed on the second porous spacer layer on a side opposite the porous active layer. The extraction module can be particularly useful for extraction of a target species from a fluid.

A method of making 1,5 pentanediamine (a.k.a. PDA, pentamethyldiamine, PMDA and cadaverine) by decarboxylation of lysine using lysine decarboxylase in the presence of sulfate, or more preferably phosphate anion salts of lysine is described. The process works at high pHs at which the decarboxylase does not function using HCL salts of lysine and at substrate concentrations not achievable with lysine HCL at the ordinary pH optima of the carboxylase. In addition, a chromatographic process for purifying the PDA from lysine and the conjugate anions of the lysine salt is described that is applicable as a general process for purifying any diamine from a product mixture comprising amino and / or carboxylic acids along with their conjugate anions, which method uses a strong anion exchange resin as the stationary phase. The method does not utilize any organic solvent and is shown to be economical and efficient for industrial scale isolation of diamines from complex mixtures containing amino and / or carboxylic acids and conjugate anions from the amine salts.

To provide a water softening device capable of removing precipitates on a cathode while suppressing electrode deterioration.SOLUTION: A water softening device 1 includes a water softening tank 3, a neutralization tank 4, an electrolytic tank 7, and a chemical input part. The water softening tank 3 softens raw water containing hardness components by means of a weakly acidic cation exchange resin in a water softening process. The neutralization tank 4 neutralizes the softened water that has passed through the water softening tank with a weakly basic anion exchange resin in a neutralization process. The electrolytic tank 7 generates alkaline electrolytic water for regeneration of the weakly basic anion exchange resin in the regeneration process. The chemical input part feeds chemicals used in the electrolytic tank cleaning process to dissolve precipitates that precipitate in the electrolytic tank into the electrolytic tank.SELECTED DRAWING: Figure 1

In this invention, the protocol to be followed for obtaining a biosurfactant extract from corn steep liquor is described, by separation using different solid polymeric matrices (liquid-solid extraction) and subsequent desorption of biosurfactant extract contained in the polymeric matrix by solid-liquid extraction The solvent used in the polymer regeneration and biosurfactant extract elution is separated by distillation or evaporation resulting in a solvent-free biosurfactant extract that is capable of reducing the surface tension of water by more than 15 units and with a critical micelle concentration (CMC), under the most favorable conditions, of less than 200 mg / L.

The present disclosure provides compositions, products, kits and methods for producing on demand hypochlorous acid solutions. The present disclosure provides a stable, dry mix product, kit or package that can be employed by an end-user to easily prepare a hypochlorous acid composition with desired features and functionality.

Provided herein are methods for purifying recombinant adeno-associated virus (rAAV) particles using chromatography in a large scale. Further provided include the rAAV particles and the pharmaceutical composition prepared by the method.

The present invention relates to methods of reducing the rate of enzymatic hydrolysis activity in compositions obtained from a purification platform, and provides purification platforms comprising a depth filtration step and / or a hydrophobic interaction chromatography (HIC) step. The present invention also discloses methods of using the purification platforms described herein and compositions, such as pharmaceutical compositions, obtained from the purification platforms.

The present invention relates to methods for polishing and enrichment of full adeno-associated virus particles by anion exchange chromatography using basic amino acids in the elution buffer.

To provide a water softening device that enables efficient regeneration of ion exchange resins. [Solution] The water softening device comprises a water softening chamber 209 that generates softened water, a neutralization chamber 210 that neutralizes the softened water, an inner circumferential diaphragm 215, a water intake section 203 that introduces raw water, a water supply section 204 that supplies the neutralized softened water to the outside, a first electrode 201, and a second electrode 202. Furthermore, the water softening chamber 209, the inner circumferential diaphragm 215, the neutralization chamber 210, and the water supply section 204 are arranged in the order of the water intake section 203 having a central axis perpendicular to the bottom surface of the water softening chamber 209, and outward in the direction of the outer circumference of a circle centered on the central axis. The weakly acidic cation exchange resin 213 includes a first weakly acidic cation exchange resin 213a and a second weakly acidic cation exchange resin 213b that has a higher acidity dissociation constant than the first weakly acidic cation exchange resin 213a. The first weakly acidic cation exchange resin 213a is located closer to the first electrode 201 than the second weakly acidic cation exchange resin 213b.

The invention provides a chromatographic method for separating pre-contraction state PVC Nanosyringes from post-contraction state PVC Nanosyringes, the method comprising: (a) providing a sample containing both pre- and post-contraction state PVC Nanosyringes; (b) loading the sample on a monolith anion exchange column having (preferably quaternary amine) binding moieties, under conditions (e.g. pH 7-8, temperature 19-25°C) wherein pre- and post-contraction state PVC Nanosyringes become bound to the column with differing affinities; (c) applying a gradient of ionic strength to the column that provides for differential elution of the pre- and post-contraction state PVC Nanosyringes, wherein the gradient is generated by incrementally (e.g. linearly) increasing the salt concentration of a mobile phase elution buffer that is applied to the column; and (d) eluting the pre- and post-contraction state PVC Nanosyringes into separate elution fractions under the gradient based on their differing affinities to the anion exchange column, and collecting fractions having the pre-contraction state PVC Nanosyringes separated from the post-contraction state PVC Nanosyringes.

The present disclosure provides processes for relates to processes for separating one or more water-soluble products from non-woody plants of the genus Hesperaloe. The processes described herein sequentially separates one or more water-soluble products from non-woody plants of the genus Hesperaloe. The process may include sequential separation and enrichment of different saponins, polyphenols, and / or organic acids from a juice derived from non-woody plants of the genus Hesperaloe. The sequential separation of water-soluble products from non-woody plants of the genus Hesperaloe provides multiple high-value products.

The application provides an ion exchange resin and a preparation method thereof. The preparation method of the ion exchange resin comprises the following steps: performing a polymerization reaction on a mixed system comprising a support material, an acrylate monomer, a crosslinking agent and an initiator to obtain a composite microsphere; performing a chloromethylation reaction on the composite microsphere, a second solvent and a chloromethylation reagent to obtain a composite chloromicrosphere; and performing an amination reaction or a sulfonation reaction on the composite chloromicrosphere to obtain the ion exchange resin. The ion exchange resin prepared by the preparation method has the advantages of high mechanical strength and strong adsorption capacity. When the ion exchange resin is applied to the purification of a crude terephthalic acid diethylene glycol ester, the ion exchange resin can be stably operated in a water system, the generation of a hydrolysis byproduct is reduced, the product purity is significantly improved, and the industrial purification of waste polyester alcoholysis products is effectively supported.

The present invention comprises a new class of high and low phosphonated aliphatic fluoropolymer rubbers (pFKM) and perfluoropolymer rubbers (pFFKM) based on FKM and FFKM, as well as methods for their preparation and their uses.

A method for separating a transition metal and Li includes a step of allowing a compound containing Li and a transition metal to react with a carboxylic acid in water, to obtain a slurry containing a first solution containing Li ions and a precipitate containing a transition metal carboxylate, and a step of separating the precipitate from the first solution. The transition metal is at least one selected from the group consisting of Ni, Co, Mn, Ti, Fe, and Cu.

To provide methods of reducing bioburden of a chromatography resin.SOLUTION: Provided herein are methods of reducing bioburden of a chromatography resin that include exposing a container including a composition including (i) a chromatography resin and (ii) a liquid including at least one alcohol to a dose of gamma-irradiation sufficient to reduce the bioburden of the container and the chromatography resin, where the at least one alcohol is present in an amount sufficient to ameliorate the loss of binding capacity of the chromatography resin after / upon exposure to the dose of gamma-irradiation. Also provided are reduced bioburden chromatography columns including the reduced bioburden chromatography resin, compositions including a chromatography resin and a liquid including at least one alcohol, and methods of performing reduced bioburden column chromatography using one of these reduced bioburden chromatography columns.SELECTED DRAWING: Figure 1

The application belongs to the technical field of lithium ion battery electrolyte, and relates to a method and system for continuously preparing high-purity liquid bisfluorosulfonylimide lithium. The application generates molten LiFSI through one-pot reaction of bischlorosulfonylimide, anhydrous hydrogen fluoride and lithium fluoride in a micro-reactor at 80-150 DEG C and 0.5-3.0 MPa; the molten salt is dropped into low-temperature dichloromethane for back extraction and crystallization; the crude product is obtained through continuous filtration, washing and drying; the crude product is dissolved in an ester solvent, and then sequentially subjected to continuous membrane filtration, resin deacidification and deep water removal to obtain high-purity liquid LiFSI product. The method realizes one-pot fluorination, salt formation and full-process continuous production from raw materials to liquid product, has the advantages of high safety, high efficiency, high product purity, stable quality and convenience for large-scale industrial production. In the prepared high-purity LiFSI liquid salt, the water content is less than 10 ppm, the free acid (calculated by HF) is less than 20 ppm, the Cl ‑ 5 ppm, the SO4 2‑ 5 ppm, and the single content of various metal impurity ions (such as calcium, iron, magnesium, sodium and potassium) is less than or equal to 2 ppm.

The present disclosure provides, in some embodiments, devices, methods, and kits for purifying extracellular vesicles (EVs) using size exclusion chromatography in tandem with cation exchange chromatography, which can be referred to as dual-mode chromatography (DMC).

Provided is a complex composition, of which positional isomers are minimized by using a N-terminus of an immunoglobulin Fc region as a binding site when the immunoglobulin Fc region is used as a carrier. Also provided are a protein complex which is prepared by N-terminal-specific binding of immunoglobulin Fc region, thereby prolonging blood half-life of the physiologically active polypeptide, maintaining in vivo potency at a high level, and having no risk of immune responses, a preparation method thereof, and a pharmaceutical composition including the same for improving in vivo duration and stability of the physiologically active polypeptide. The protein complex may be usefully applied to the development of long-acting formulations of various physiologically active polypeptide drugs.

The utility model relates to the technical field of Kathon production, in particular to a mixing system for Kathon production, which comprises a first dissolving kettle and a second dissolving kettle, a discharge port of the first dissolving kettle is communicated with a first impurity removal device, a discharge port of the second dissolving kettle is communicated with a second impurity removal device, a discharge port of the first impurity removal device is communicated with a first buffer kettle, and a discharge port of the second buffer kettle is communicated with a second buffer kettle. A discharge port of the second impurity removal device is communicated with a second buffer kettle, a discharge port of the first buffer kettle and a discharge port of the second buffer kettle are respectively communicated with a mixing kettle, and a discharge port of the mixing kettle is communicated with a product storage tank. The mixing system with the structure is reasonable in design, and the purity and the stability of Kathon products are improved.

Compositions and methods are provided for producing crystalline forms of organic acids or salts or lactones thereof from an aqueous solution. More specifically, methods are provided for producing a crystalline form of a salt of mevalonic acid (also referred to as X-MVA) from an aqueous solution, comprising subjecting the aqueous solution comprising said X-MVA to a purification step to produce a purified solution and crystallizing said X-MVA from said purified solution by water solvent crystallization. Methods are also provided for producing mevalonolactone from an aqueous solution comprising X-MVA, comprising subjecting the aqueous solution comprising said X-MVA to cation exchange thereby converting said aqueous solution comprising X-MVA to an aqueous solution comprising mevalonolactone (MVL). Methods are also provided for producing mevalonolactone monohydrate crystals.

The present invention relates to a method for preparing a plasma sample comprising amyloid beta peptides for analysis by mass spectrometry, comprising the steps of:a) contacting said plasma sample with a denaturing agent, b) performing a first solid phase extraction step on the solution obtained in step a) to recover a first eluate, c) performing a second solid phase extraction step on said first eluate obtained in step b) to recover a second eluate, and d) drying said second eluate obtained in step c) and processing it for analysis by mass spectrometry, wherein the solution obtained in step d) comprises intact amyloid beta peptides Aβ40 and Aβ42.

The present disclosure to an electrolyte membrane containing a polyfluorene-based ionomer, and more particularly, to an electrolyte membrane containing a polyfluorene-based ionomer which has a fluorene main chain composed of only a carbon-carbon bond. The electrolyte membrane containing the polyfluorene-based ionomer has high proton conductivity, excellent chemical durability, excellent mechanical property, and excellent volume stability.

This invention provides a method for separating and purifying uronic acid polysaccharides, belonging to the field of polysaccharide separation and purification technology. This invention utilizes the electrostatic adsorption of sodium ions in an aqueous sodium chloride solution around the carboxyl anions of uronic acid units, forming a charge shielding layer. Chloride ions in the aqueous sodium chloride solution are also adsorbed around the dextran gel via electrostatic interaction, effectively shielding the electrostatic attraction between the uronic acid units and the dextran gel, reducing the non-specific adsorption of uronic acid units and the dextran gel, making the uronic acid polysaccharides easy to separate and with high purity. Furthermore, sodium and chloride ions in the aqueous sodium chloride solution are strongly hydrated ions, preferentially binding to the hydroxyl groups on the dextran gel and the carboxyl groups on the uronic acid units, occupying hydrogen bond sites, disrupting the existing hydrogen bonds between the uronic acid units and the dextran gel, preventing the formation of new hydrogen bonds, reducing the adsorption of uronic acid units in the dextran gel, making the uronic acid polysaccharides easy to separate and with high purity.