430results about "Complex ion-exchangers" patented technology

The invention discloses a human albumin binding peptide 1E3 and application of the human albumin binding peptide 1E3 in promoting purification of human albumin, and belongs to the technical field of polypeptides. The human albumin binding peptide comprises an amino acid sequence as shown in SEQ ID NO. 1; and / or an amino acid sequence of a fusion protein with the same function, which is obtained by connecting tag protein to the N terminal and / or C terminal of the amino acid sequence as shown in SEQ ID NO.1. The human albumin binding peptide has extremely high affinity with human albumin and can be used for separating and purifying a human albumin solution, and the purity of the purified human albumin far exceeds the pharmacopoeia standard and can reach 99.99% or above. The method is good in safety and stable in process, and has a wide application prospect in the aspect of separation and purification of the human albumin.

The invention discloses a method for extracting a target product from a milk raw material, and relates to the technical field of food processing. According to the method, before strong anion exchange chromatography is carried out, a synergistic pretreatment step of ultrafiltration and conductivity adjustment is introduced, so that acceleration, synergism, quality improvement and cost reduction of the chromatography process are realized, and the technical bottlenecks of low speed, easiness in pollution, instability and the like of the traditional ion exchange chromatography during treatment of milk raw materials are overcome; and a solution is provided for high-efficiency, high-purity and large-scale production of the beta-lactoglobulin and the alpha-lactalbumin. According to the method, strong anion exchange chromatography and weak anion exchange chromatography can be combined, so that the extraction purity of beta-lactoglobulin and alpha-lactalbumin can be further improved; in addition, the method can synchronously realize high-efficiency extraction of various target substances such as casein micelles, lactose and milk mineral salts, so that the additional value and resource utilization efficiency of milk source byproducts are greatly improved.

A full solid-waste micro-expansive concrete and its preparation method are provided, and relate to the field of concrete material technologies. The full solid-waste micro-expansive concrete includes the following components in parts by weight: 920-1060 parts of a coarse aggregate, 460-582 parts of a fine aggregate, 11-19 parts of an expansive agent, 389-512 parts of a modified solid-waste-based cementitious material, and 231-268 parts of water. The coarse aggregate is coal gangue. The fine aggregate is waste ceramic. The modified solid-waste-based cementitious material includes a solid-waste-based clinker, modified polyvinyl alcohol, and a modified anion exchange resin. The solid-waste-based clinker is prepared from the coal gangue, carbide slag, and desulfurization gypsum. The expansive agent is obtained by calcining the carbide slag. The concrete prepared can ensure the initial strength of the concrete, and guarantee continuous micro-expansion in the later stage, compensating for shrinkage and thereby increasing the overall strength of the concrete.

Systems and methods for generating radionuclides, such as radium-224. Systems herein may include a first cartridge having a first opening, a second opening, and a chamber therebetween having a first resin having affinity for thorium-228 and bismuth-212; a second cartridge having a first opening, a second opening, and a chamber therebetween having a second resin having affinity for thorium-228 and bismuth-212, a third cartridge having a first opening, a second opening, and a chamber therebetween comprising a third resin having affinity for thorium-228 and bismuth-212, a fourth cartridge having a first opening, a second opening, and a chamber therebetween having a third resin having affinity for lead-212; wherein a continuous flow path is formed from a top of the first cartridge though the second cartridge, through the third cartridge, and to a bottom of the fourth cartridge during system use.

The invention relates to the technical field of biomacromolecule separation and purification engineering, and discloses a chromatographic separation method for removing endotoxin in a vaccine preparation, which comprises the following steps: preparing an equilibrium buffer solution containing citrate with specific concentration and L-arginine hydrochloride; providing a mixed mode chromatography medium with hydrophobic and ion exchange complex ligands and balancing with a buffer; according to the method, through the synergistic effect of 8.0-12.0 mmol / L of citrate and 150-250 mmol / L of L-arginine hydrochloride, the endotoxin micelle structure is destroyed and is induced to be dissociated into monomers, and meanwhile, L-arginine is used for inhibiting monomer aggregation, so that the monomers are diffused into inner holes of the medium in a small size to be adsorbed, and the endotoxin micelle structure is separated into the medium. Therefore, the problem of separation caused by size overlapping of endotoxin micelles and antigens is solved, and double breakthrough of deep removal of endotoxin and high recovery rate of antigens is realized.

The invention provides a production process of SEMI-G5-grade high-purity NMP (N-Methyl Pyrrolidone), which is characterized in that metal ions, magnetic foreign / non-magnetic foreign particulate matters and the like of conventional industrial-grade NMP are completely removed by adopting a multi-section rectification, resin adsorption and precise filtration process, so that the SEMI-G5-grade NMP reaches the SEMI-G5 grade in an ultra-clean high-purity reagent; and the use requirements on the ultra-clean high-purity NMP in the fields of semiconductors, integrated circuits and novel display are met.

To extend the lifespan of plating baths used for forming metal oxide films and to enable more efficient use of the plating baths. [Solution] The method for forming a metal oxide film comprises the steps of forming a metal oxide film on the surface of a plated object using a plating bath containing a metal compound that forms the film and a boron compound that acts as a reducing agent, and regenerating the plating bath using an adsorption filter that adsorbs boric acid.

A waste extraction system that includes a column set positioned between and fluidly coupled to an upstream segment of a main waste pathway and a column effluent tank, the column set having a uranium adsorption column fluidly coupled to a supporting adsorption column along the main waste pathway. The uranium adsorption column is upstream the supporting adsorption column. The waste extraction system also includes a strip waste pathway extending from the uranium adsorption column to the column effluent tank bypassing the supporting adsorption column, an anion exchange resin housed in the uranium adsorption column, and a cation exchange resin housed in the supporting adsorption column.

The present invention provides, inter alia, a method for regenerating an ion exchange resin which is at least partially loaded with fluoride ions in an ion exchange vessel, wherein said ion exchange resin is a metal ion-doped chelating resin comprising amino-phosphonate groups.

The present invention relates to methods and devices for isolating one or more biomolecules of interest from a biological composition. Specifically, the invention relates to the separation of one or more target biomolecules from a biological composition by dynamic packed bed adsorption using a separation medium comprising porous rod-like particles attached to the target molecules and filtration using a filter having a pore size of at least 1.5 [mu] m.

A substantially animal-protein free (APF) composition comprises a botulinum toxin serotype A neurotoxin having a molecular weight of 150 kDa, comprising one nanogram or less of residual nucleic acid for each milligram of the botulinum neurotoxin component, wherein the botulinum toxin serotype A neurotoxin is obtainable by a process comprising the steps of (a) providing a substantially APF fermentation medium; (b) fermenting Clostridium botulinum bacteria in the fermentation medium, and; (c) recovering the biologically active botulinum neurotoxin from the fermentation medium by contacting the fermentation medium with an anion exchange chromatography media followed by contacting an eluent from the anion exchange chromatography medium with a cation exchange chromatography media.

The present application provides a chromatographic baseline suppression device and a manufacturing method thereof, the chromatographic baseline suppression device comprises a container and two ion exchange membranes, the two ion exchange membranes divide the inside of the container into a first part, a second part and a third part, the second part has an inlet and an outlet; the chromatographic baseline suppression device further comprises two electrodes respectively in the first part and the second part, the electrodes comprise a matrix porous material layer and a polymer layer, the matrix porous material layer is conductive and is connected with the ion exchange membrane. The present application has the advantages of small noise and the like.

It is provided a process for extracting nickel sulfate from mining ores, such as serpentine, comprising the steps of conducting a magnetic separation of the mining ores producing a magnetic fraction and a non magnetic fraction; leaching the non magnetic fraction with HCI producing a slurry comprising metals chloride; filtrating the slurry producing a metals chloride liquor; purifying the metals chloride liquor producing a magnesium chloride solution; separating an iron-nickel cake from the magnesium chloride solution; leaching the cake together with the magnetic fraction producing a metallic sulfate solution; extracting nickel and cobalt from the metallic sulfate solution by a ion exchange resin extraction and stripping producing an inorganic stripped phase; submitting the inorganic stripped phase to a liquid-liquid extraction producing a nickel concentrated phase; and evaporating and drying the nickel concentrated phase to recuperate nickel sulfate.

The present application relates to the technical field of ion detection, in particular to a high-efficiency anti-interference ion chromatography detection device and method, through sample pretreatment module, the original sample is filtered, enriched and interference shielding processing, effectively removes impurities and shields interfering ions, at the same time, the chromatographic separation module realizes the simultaneous separation of anions and cations under constant temperature conditions, avoids the problems of chromatographic peak overlap and tailing under the traditional single separation mechanism, significantly improves the detection stability and accuracy of complex matrix samples; the eluent supply module generates high-purity eluent online to avoid impurity pollution caused by manual preparation, the detection module reduces the background conductance in the ion flow and converts the ion concentration change into an electrical signal, effectively reduces the baseline noise, meets the high-sensitivity detection requirement of trace ions; the central control unit controls the cooperative work of each module, greatly shortens the analysis time, and is suitable for rapid detection of large quantities of samples.

The application discloses a process for separating and purifying a mixed solution of HCl and NaCl by using a chromatographic technique. Liquid containing HCl and NaCl is loaded into a chromatographic column containing an anion exchange resin, adsorbed, washed, desorbed, and the effluent is collected. The application uses acid retardation and ion exchange mechanism, adopts fixed bed technology, and realizes the purpose of removing NaCl and separating and purifying high-concentration HCl in one step. The whole process does not use acid and alkali, the Na content in the product liquid is lower than the ion chromatography detection limit, the yield of HCl reaches more than 95%, and the average concentration reaches more than 50%. + The application also uses ultrapure water as a desorbent, so that the product elution and resin regeneration are completed at the same time, and the process is simplified.

The disclosure provides preparation and application of an anionic resin with high selective adsorption of ergothioneine, belonging to the field of biochemical engineering. A polyamine-type epoxy weak-base anionic resin is used as a carrier for the first time. Ni atoms are loaded onto the target resin carrier in the form of [NiCl4]2— ions, followed by in situ reduction and rinsing regeneration to prepare a metal Ni nanoparticle-loaded polyamine-type epoxy weak-base anionic resin. The preparation process is simple and the stability is good. The disclosure has strong selective adsorption for ergothioneine in fermentation broths and the facile elution. The disclosure can be widely applied in purification processes for ergothioneine preparation through fermentation and enzymatic catalysis.

The present disclosure is generally directed to polymers comprising repeat units having the structures of Formulae 1, 2, and 3. These polymers are used in methods of removing contaminants, including PFAS and heavy metals, from an aqueous fluid comprising contacting the aqueous fluid with the polymer and in methods of catalyzing an organic transformation comprising contacting a fluid with a catalytic amount of the polymer.

The utility model discloses a desalting device for production of methyltrimethoxysilane, which comprises a base, vertical plates fixedly connected to the left end and the right end of the upper surface of the base, a temporary storage box arranged between the two vertical plates, two mounting discs fixedly connected to the surface of the temporary storage box, and the two mounting discs are oppositely distributed up and down. A plurality of automatic telescopic rods are fixedly connected to the upper surface of the base, limiting assemblies are arranged at the upper ends of the automatic telescopic rods and the lower surface of the lower mounting disc, a built-in box is fixedly connected to the interior of the temporary storage box, two liquid guide pipes are fixedly connected to the lower surface of the temporary storage box, and long plates are arranged in the built-in box and the temporary storage box correspondingly; the surfaces of the long plates are fixedly connected with a plurality of ion exchange resin storage boxes, and the faces, away from the ion exchange resin storage boxes, of the two long plates make close contact with the front inner wall of the temporary storage box and the rear inner wall of the built-in box correspondingly. Through the structure, the occupied area of the desalting device is reduced, and the convenience of replacing articles in the device is improved.

The present invention relates to a method for forming a metal oxide coating film, which can prolong the service life of a plating solution for forming a metal oxide coating film and can effectively use the plating solution. This method for forming a metal oxide coating film is provided with: a coating film forming step for forming a metal oxide coating film on the surface of an object to be plated by means of a plating solution containing a compound of a metal that forms the coating film and a compound of boron as a reducing agent; and a regeneration step for regenerating the plating solution using an adsorption filter that adsorbs boric acid.

The application discloses a processing equipment for crude heparin sodium and belongs to the field of heparin sodium preparation, which comprises a shell, a liquid discharge port arranged at the lower end of the shell, a cover one fixed at the upper end of the shell, a liquid inlet arranged on the cover one, and an adsorption assembly arranged in the shell. The adsorption assembly comprises a plurality of supporting pieces fixed to the inner bottom wall of the shell, a filter cartridge clamped to the upper ends of the supporting pieces, a cavity and a hollow cavity arranged in the filter cartridge, and a cover two fixed to the upper end of the filter cartridge and sealing the cavity. The cavity for placing the resin is arranged in the filter cartridge, the resin is confined in the filter cartridge, direct contact with the high-speed rotating stirring paddle is avoided, and the resin is not damaged by the high-speed rotating stirring paddle. The paddle in the hollow cavity can actively and continuously suck the liquid from the bottom of the filter cartridge, and force the liquid to penetrate the resin bed in the radial direction. The radial flow has a large cross-sectional area, a short path, and a more uniform fluid distribution, and the utilization rate of the resin is higher.

The invention provides a deleading agent FT236 and a preparation method and application thereof, the deleading agent FT236 is prepared by taking cellulose nanocrystals CNC as a matrix, diethylenetriamine pentaacetic acid DTPA as a collecting agent, gamma-mercaptopropyltrimethoxysilane MPTMS as a cross-linking agent and chitosan quaternary ammonium salt HTCC as a flocculating agent through the steps of main reaction and post-treatment. The nano structure of the CNC substrate can uniformly load DTPA and MPTMS to provide a stable framework; a collecting agent DTPA is extremely high in chelating capacity for lead ions and is not combined with silver ions, and loss of precious metal is avoided; mPTMS can strengthen the structural stability of the medicament; the HTCC has flocculation activity and can promote agglomeration of the nanoparticles.

A cartridge attachment structure includes: a cover whose one end is attached to a pivot shaft for opening and closing a storage space; a support member attached to the pivot shaft and supporting a cartridge; and a pair of lifting-up members attached to the cover such that the cartridge can be sandwiched along the side surface of the cartridge. A groove is formed on each of the opposing inner surfaces of the lifting-up members to receive a projection 16 formed on the side surface of the cartridge. The groove is curved such that the bottom surface of the cartridge moves away from the support member when the protrusion slides along the groove as the cover rotates about the pivot shaft.

The present disclosure relates to an automated device and methods to produce a highly purified alpha-emitting radioisotope Pb-212 from a pre-filled column of a parent isotope Ra-224 for use in targeted alpha-particle therapy.

The present disclosure provides purification platforms comprising a depth filter step and / or a hydrophobic interaction chromatography (HIC) step. Also disclosed herein are methods of using the purification platforms described herein and compositions obtained therefrom, such as pharmaceutical compositions.

The present disclosure provides a simple and versatile process suitable for large-scale production of alpha emitters. It does not require irradiation of the source material and it is based on aqueous solutions and a strong cation exchange resin.

The invention discloses a multi-mode liquid chromatography filler of ionic liquid hydrogel modified silica gel and preparation and application thereof, and the method comprises the following steps: fully dissolving an ionic liquid hydrogel monomer and a cross-linking agent in a polar aprotic organic solvent, adding silica gel, carrying out ultrasonic treatment, and standing; adding an initiator, stirring, condensing, refluxing, stirring and reacting at 70-80 DEG C in an inert atmosphere; and drying under stirring, centrifugally washing and drying. According to the invention, the problems of single functional group, tedious modification steps, low ligand density and poor high-pressure stability in the existing method are solved. The preparation method is simple and convenient, efficient modification of hydrogel on the surface of silica gel can be achieved, and the prepared liquid chromatography packing has good separation selectivity on nucleoside / basic groups, organic acid compounds, alkaloid, antibiotics, aniline compounds, anion-containing compounds and the like; and chromatographic separation in a hydrophilic chromatographic mode, a hydrophobic chromatographic mode and an ion exchange chromatographic mode can be realized.

Ion exchange membranes (e.g., anion exchange membranes) and methods of using the membranes are described. The ion exchange membranes are multi-modal ion exchange membranes containing a plurality of multi-modal exchange ligands. The membranes can achieve high dynamic and equilibrium binding capacities at solution conductivities typical for production of biologics (e.g., greater than about 10 mS / cm) and can provide excellent binding at high flow rates. Systems incorporating the membranes can dramatically increase isolation and purification speeds. Membranes are disclosed for use in production of biologics.

The present invention pertains to the purification of soluble complement receptor proteins and variants thereof by hydrophobic interaction chromatography (HIC) using a chromatographic material with a particle size of less than 60 µm.

The subject invention pertains to multimodal chromatography resins comprising anionic and cationic ligands on each resin particle and methods of synthesizing the multimodal chromatography resin by contacting a base with quaternary amines and / or tertiary amines on the surface of a substrate. The multimodal resins can be tuned by changing the ratios of cation to anions. The multimodal chromatography resins can be used for purifying proteins.

A production method for water for pure water includes adding polyaluminum chloride to raw water to produce first treated water, and filtering the first treated water in two or more stages including filtering with a filtration membrane device in a preceding stage to produce first concentrated water, and filtering the first concentrated water with a filtration membrane device in a subsequent stage. The water for pure water is produced as permeated water produced by the filtering in the two or more stages.