Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for extracting blue-green algae extracellular polymeric substance in grading manner

An extracellular polymer and fractional extraction technology, applied in the field of cyanobacterial extracellular polymer extraction, can solve the problems of large differences in extraction operating conditions, poor comparability of results, etc., to ensure cell integrity, improve yield, and high extraction efficiency. Effect

Inactive Publication Date: 2013-09-18
NANJING INST OF GEOGRAPHY & LIMNOLOGY
View PDF3 Cites 10 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although there are many studies on extracellular polymers, the results obtained by different researchers are not comparable, partly because of the large differences in the extraction conditions they adopted, and another important reason is that they all use extracellular polymers Taken as a whole, no fractional extraction of extracellular polymers was performed
However, there are no relevant reports on fractional extraction of cyanobacterial extracellular polymeric substances

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for extracting blue-green algae extracellular polymeric substance in grading manner
  • Method for extracting blue-green algae extracellular polymeric substance in grading manner
  • Method for extracting blue-green algae extracellular polymeric substance in grading manner

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Take a sample of cyanobacteria water body in Taihu Lake in summer. The biomass of cyanobacteria is 0.8g / L. After the cyanobacteria water sample is collected, it is placed in a refrigerator at 4°C, and the extracellular polymer is fractionated and extracted within 12 hours. The specific operation steps are as follows:

[0031] (1): Extraction of soluble and loosely bound extracellular polymers from cyanobacteria

[0032] Take a certain amount (50ml) of cyanobacteria water samples and centrifuge at 2500g centrifugal force for 15min at 4°C. The supernatant collected is SL-EPS; Centrifuge at 4500g for 15 minutes, and the collected supernatant is LB-EPS; add 0.05% NaCl buffer solution to the original volume of the solid sample, and proceed to the next step.

[0033] (2): Extraction of tight-binding extracellular polymers from cyanobacteria

[0034] Use 40% NaOH solution to adjust the pH of the algal cells to be extracted to 9.0, equilibrate for 10 minutes, and then place t...

Embodiment 2

[0041] Take the cyanobacteria water samples in Taihu Lake in autumn. The cyanobacteria biomass is 0.5g / L. After the cyanobacteria water samples are collected, they are placed in a 4°C refrigerator, and the extracellular polymers are fractionated and extracted within 12 hours. The specific operation steps are as follows:

[0042] (1): Extraction of soluble and loosely bound extracellular polymers from cyanobacteria

[0043] Take a certain amount (50ml) of cyanobacteria water samples and centrifuge at 2000g centrifugal force for 15min at 4°C. The collected supernatant is SL-EPS; add 0.07% NaCl buffer solution to the original volume of solid samples, Centrifuge at a centrifugal force of 5000g for 15 minutes, and the collected supernatant is LB-EPS; add 0.07% NaCl buffer solution to the original volume of the solid sample, and proceed to the next step.

[0044] (2): Extraction of tight-binding extracellular polymers from cyanobacteria

[0045] Use 35% KaOH solution to adjust the...

Embodiment 3

[0050] Microcystis aeruginosa was cultivated indoors to obtain a culture solution of Microcystis aeruginosa, and the algae biomass was determined to be 0.15 g / L. Samples were subjected to the following steps:

[0051] Take a certain amount (50ml) of the culture solution sample and centrifuge at 2500g centrifugal force for 15min at 4°C, and the collected supernatant is SL-EPS; add 0.08% NaCl buffer solution to the original volume of the solid sample, Centrifuge at 4500g for 15 minutes, and the collected supernatant is LB-EPS; add 0.08% NaCl buffer solution to the original volume of the solid sample, and proceed to the next step.

[0052] (2): Extraction of tight-binding extracellular polymers from cyanobacteria

[0053] Use 40% KaOH solution to adjust the algae cells to be extracted to pH 8.0, balance for 10 minutes, and then place the algae liquid to be extracted at 55 o C constant temperature hot water reaction for 30min. Take it out and cool it immediately, and centrifuge...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to an operation method for extracting a blue-green algae extracellular polymeric substance in a grading manner. The method comprises the following steps of: firstly, carrying out middle / low-speed eccentric shearing to obtain a soluble and loosely combined type extracellular polymeric substance; and secondly, extracting a tightly combined type extracellular polymeric substance in a mode of subalkaline hot bath with the combination of high-speed eccentric shearing. The physical and chemical analysis result shows that the soluble and loosely combined type extracellular polymeric substance contains a small amount of organisms, and the tightly combined type extracellular polymeric substance contain a great amount of organisms; and three-dimensional fluorescence and infrared spectroscopy result shows that the soluble extracellular polymeric substance is only composed of polysaccharide, protein substances and humus substances, and the loosely combined and tightly combined extracellular polymeric substances only consist of polysaccharide and protein substances. The invention discloses an operation method for grading extraction of the blue-green algae extracellular polymeric substance. The method has the advantages that the operation is simple, the cyanobacterium is free of damage, the cyanobacterium is analyzed in different grades, and the like; and the method has high guiding significance in industrial application of the blue-green algae extracellular polymeric substance.

Description

technical field [0001] The invention relates to a method for extracting cyanobacterial extracellular polymers, in particular, the method can realize the graded extraction operation of extracellular polymers, thereby obtaining cyanobacterial extracellular polymers with different organic components and contents, and belongs to the technical field of bioengineering . Background technique [0002] In recent years, with the intensification of human activities and eutrophication, lake cyanobacteria blooms frequently broke out, causing adverse effects on human health and the ecological environment. Cyanobacterial extracellular polymer is a general term for a series of macromolecular organic substances produced during the growth and reproduction of cyanobacteria cells, mainly derived from cell secretion, cell lysis, cell adsorption and foreign substances. Cyanobacterial extracellular polymers form cyanobacterial colonies by adhering to individual cyanobacteria, thereby promoting th...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C07K1/14C08B37/00
Inventor 江和龙徐华成
Owner NANJING INST OF GEOGRAPHY & LIMNOLOGY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com