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Preparation method of compound microbial algaecide

A compound algaecide and microorganism technology, applied in the field of environmental protection, can solve the problems of poor water bloom control effect and achieve the effect of low price and low preparation cost

Inactive Publication Date: 2014-11-12
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

More and more studies have found that the above methods have their own shortcomings, and the single use of the above methods is less effective in controlling algae blooms

Method used

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  • Preparation method of compound microbial algaecide
  • Preparation method of compound microbial algaecide
  • Preparation method of compound microbial algaecide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1 Screening of alginolytic bacteria (R1)

[0027] 1.1 Enrichment: Inoculate 200uL of collected water samples into 100mL LB medium, enrich and culture at 37°C, 200rpm for 24h. Take 5 mL of the enriched cultured bacterial liquid and centrifuge to remove the culture medium, wash the bacterial cells with sterile water for 3 times and suspend in 10 mL sterile water to obtain the enriched cultured bacterial liquid.

[0028] 1.2 Preliminary screening: inoculate 25 mL of Microcystis aeruginosa liquid (OD 680 =1.0), mixed evenly and cultivated in the artificial intelligence incubator for 5 days (in the artificial intelligence incubator, the light intensity is 2000Lx, the light-dark time ratio is 12:12, and the temperature is 25±1℃), the Microcystis aeruginosa liquid appears yellow Phenomenology: Microcystis aeruginosa liquid turns from green to yellow. The more obvious the yellow color, the better the algae-dissolving effect of the bacterial liquid.

[0029] Take 1mL...

Embodiment 2

[0032] Example 2 Screening of algal toxin-degrading bacteria (M1)

[0033] 2.1 Initial screening: Take 1mL of the bacterial enrichment culture solution obtained in step 1.1 and inoculate it into 20mL of MM (glucose-free) liquid medium containing the crude Microcystin aeruginosa extract (the concentration of the cyanotoxin is not counted) (the cyanotoxin crude The volume ratio of the extract to the glucose-free MM liquid medium is 1:1), then cultured on a shaker in the dark (200rpm, 37°C), observe the growth of the strains and select the strains with the best growth for re-screening.

[0034] 2.2 Re-screening: Add 20mg of MC-LR to 1L of glucose-free MM medium, use this medium to continue culturing the culture in 2.1, 200rpm, 37°C for 48h in the dark, and select the bacteria with the best growth ability. It is Microcystin aeruginosa degrading bacteria, and it is named as M1.

[0035] 2.3 Molecular biological identification: see 1.4 for the method, and the identification resul...

Embodiment 3

[0036] Example 3 Screening of Extracellular Polymer-Secreting Strains (DT)

[0037] 3.1 Activation of single colonies: Randomly take a few single colonies stored in the laboratory, first inoculate them into 5mL LB liquid medium at 200rpm, cultivate at 37°C for 12h, and then use the culture solution as the seed solution (OD 600 =0.5), to be used.

[0038] 3.2 Cultivation of a single colony: Take 1mL of different seed solutions and inoculate into 100mL MMDT medium (sucrose 5g / L, glucose 2g / L, maltose 2g / L, yeast extract 5g / L, NH 4 Cl 1.5g / L, (NH 4 ) 2 SO 4 1.5g / L, FeCl 3 0.01g / L, MnCl 2 0.01g / L, pH 8.0), cultured at 30°C for 24h to obtain the MMDT culture solution of each bacteria.

[0039] 3.3 Determination of flocculation activity: Take 1 mL of the MMDT culture solution obtained in 3.2 and add it to 40 mL of kaolin suspension (10 g / L), then add 1 mL of CaCl 2 Solution (10g / L), 200rpm, 2min to make it completely mixed, after standing for 30min, take 4mL supernatant ...

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Abstract

The invention discloses a preparation method of a compound microbial algaecide. The method comprises the following steps: reasonably combining separated and purified extracellular polymeric substances, kaolin and the like so as to prepare a compound microbial flocculant; then fossilizing separated alga dissolving bacteria and algal toxin degrading bacteria; finally, reasonably compounding the compound microbial flocculant with the fossilized alga dissolving bacteria and algal toxin degrading bacteria so as to prepare the compound microbial algaecide. The compound microbial algaecide is good in alga killing effect and algal toxin degrading effect; source materials adopted during the preparation of the compound microbial algaecide are low in cost, so that the compound microbial algaecide is low in preparation cost and harmless to natural ecosystems. The prepared compound microbial algaecide is organically integrated with functions of flocculation-type alga removing, microbial alga dissolving and micro-biological degradation of algal toxin, so that the safe and efficient treatment of water blooms is realized.

Description

technical field [0001] The invention belongs to the technical field of environmental protection, and in particular relates to a preparation method of a microbial compound algicide. Background technique [0002] Large and medium-sized lakes in my country (>50km 2 ) accounts for 80% of the total area of ​​lakes in the country and is an important source of water. However, among more than 20,000 natural lakes, 75% of them are polluted by algae growths, which make fish and other organisms unable to survive, and become dead lakes and die out, among which Microcystis aeruginosa pollution is the most common. Therefore, the development of methods for controlling algae blooms is an important direction in the field of environmental protection research. How to achieve rapid, efficient and safe control of toxic algal blooms with low input costs is the key to judging whether a method is good or bad. However, factors such as the single algae removal effect of existing methods limit t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/14C12N11/10C02F3/34C12R1/01C12R1/07
Inventor 孙朋飞赵宇华王冠卢丽玲
Owner ZHEJIANG UNIV
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