The invention relates to an environmentally-friendly quick drying aqueous glass imitation card transfer paint and a preparation method as well as application thereof. The paint comprises the following component raw materials in parts by weight: 80-95 parts of aqueous resin, 0-8 parts of cosolvent, 0-8 parts of de-ionized water, 0-3 parts of filming auxiliary agent, 0-3 parts of anti-scratch agent, 0-0.5 part of moistening agent, 0-0.2 part of rheological control agent and 0-0.3 part of defoaming agent, and can be used for manufacturing glass imitation card paper or glass imitation copper plate paper. Compared with the prior art, the paint has the advantages of environmental friendliness, low smell, low VOC (Volatile Organic Compound), cost advantage, low production cot, high solid content, high drying speed, superior stripping property, high smoothness, high glossiness, simple application process, capability of recycling a transfer thin film for multiple times, no need of passing through a large-diameter mirror chromium plating drying cylinder like the conventional glass card and high printing adaptability.