Method for detecting lipid of euphausia superb oil in real time by rapidly evaporating ionization mass spectrum

An Antarctic krill oil and ionization mass spectrometry technology, which is applied in the field of real-time detection of lipids in Antarctic krill oil, can solve the problems of inability to realize real-time mass spectrometry detection, cumbersome processing, inapplicability, etc., so as to promote the development and real-time detection of shrimp oil. Fast, real-time mass spectrometry detection

Active Publication Date: 2018-11-16
ZHEJIANG GONGSHANG UNIVERSITY +1
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Problems solved by technology

[0005] Therefore, the existing lipidomics profile detection methods have the problems of cumbersome sample pretreatment, inability to realize real-time mass s

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  • Method for detecting lipid of euphausia superb oil in real time by rapidly evaporating ionization mass spectrum
  • Method for detecting lipid of euphausia superb oil in real time by rapidly evaporating ionization mass spectrum
  • Method for detecting lipid of euphausia superb oil in real time by rapidly evaporating ionization mass spectrum

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Embodiment Construction

[0022] The present invention will be further described below in conjunction with the accompanying drawings, but not as a basis for limiting the present invention.

[0023] The following are experimental examples of the present invention.

[0024] Experimental example.

[0025] 1.1 Main instruments and devices

[0026] Xevo G2-XS quadrupole time-of-flight mass spectrometer: American Waters company product, equipped with fast evaporation ion source; Pump11elite needle pump injection injector: American Harvard company product; Milliplus 2150 ultrapure water treatment system: American Millipore company product; Masslynx 4.1 data acquisition software and LiveID statistical analysis software: products of American Waters Company; others are commonly used laboratory instruments and equipment.

[0027] 1.2 Main materials and reagents

[0028] Antarctic krill: product of Liaoyu Antarctic Krill Technology Development Co., Ltd.; methanol, acetonitrile, isopropanol: all of chromatograph...

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Abstract

The invention discloses a method for detecting lipid of euphausia superb oil in real time by rapidly evaporating ionization mass spectrum. The method comprises the following steps: A, extracting euphausia superb oil through alcohol; B, ionizing the euphausia superb oil, then introducing a mass spectrometer and performing detection to obtain a mass spectrum profile map; and C, comparing the mass spectrum profile map through MassLynx, and then determining a lipidomic profile of samples. The method is capable of achieving real-time mass spectrum detection without the need of carrying out pretreatment on the samples, is applicable to lipidomic detection of foods, and is capable of accelerating the development of shrimp oil which takes euphausia superb as a raw material.

Description

technical field [0001] The invention relates to a method for detecting lipids in Antarctic krill oil, in particular to a real-time method for detecting lipids in Antarctic krill oil by rapid evaporation ionization mass spectrometry. Background technique [0002] Lipidomics is another rapidly developing hot field after genomics and proteomics. role analysis. With the development of soft ionization ionization technology and high-resolution mass spectrometry technology and its application in lipid analysis, rapid and high-precision analysis and detection of various trace lipids in biological samples has been realized, which has greatly promoted the Scale, holistic lipid analysis. In recent years, the application of mass spectrometry technology has become more and more extensive, and researchers have also put forward higher requirements for high-throughput and rapid detection technology of mass spectrometry. However, biological tissue samples cannot be directly used for high-...

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Application Information

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IPC IPC(8): G01N27/62
CPCG01N27/62
Inventor 沈清邓丰田张燕平王苢轩
Owner ZHEJIANG GONGSHANG UNIVERSITY
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