Application of hexagonal boron nitride nanosheet in preparing matrix solution and mass spectrometric detection method

A technology of hexagonal boron nitride and nanosheets, which is applied in the field of mass spectrometry and can solve problems to be improved, etc.

Inactive Publication Date: 2017-06-09
INST OF CHEM CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] However, matrices for matrix-assisted laser desorption / ionization mass spectrometry and methods based on matrix-assisted laser desorption / ionization mass spectrometry for the analysis of small molecules still need to be improved

Method used

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  • Application of hexagonal boron nitride nanosheet in preparing matrix solution and mass spectrometric detection method
  • Application of hexagonal boron nitride nanosheet in preparing matrix solution and mass spectrometric detection method
  • Application of hexagonal boron nitride nanosheet in preparing matrix solution and mass spectrometric detection method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] Example 1 Matrix solution background peak test

[0061] Using water as a solvent, a matrix solution with a concentration of 0.25 mg / ml hexagonal boron nitride nanosheets was prepared. The mass spectrometry conditions are: voltage: accelerating voltage: 19.000kv; delay extraction voltage: 14.920kv; reflector voltage: 20.000kv; lens voltage: 7.000kv; frequency: 1.000Hz; laser energy: 75-80%; ; Negative ion mode. refer to image 3 , in the m / z range of 0 to 1000, there is no matrix-related mass spectral signal. Therefore, the use of matrix solutions containing hexagonal boron nitride nanosheets for mass spectrometry detection of test samples, including complex systems, will not cause interference.

Embodiment 2

[0062] Example 2 Small molecule mass spectrometry analysis

[0063]Mass spectrometry analysis of solutions containing different small molecules was performed using matrix solutions containing hexagonal boron nitride nanosheets.

[0064] Take the prepared tryptophan solution (Trp) with a concentration of 1 mmol / L respectively ( Figure 4 A) Leucine solution (Leu), ( Figure 4 B), aspartic acid solution (Asn) ( Figure 4 C), glutamic acid solution (Glu) ( Figure 4 D), arginine solution (Arg) ( Figure 4 E) 1 μL of each was mixed with 1 μl of the matrix solution containing hexagonal boron nitride nanosheets (concentration of 0.1 mg / mL). The solvent of the above solution is an aqueous methanol solution with a volume ratio of 1:1. The above amino acid-containing solutions were mixed with the matrix solution evenly, and then 1 μL of the mixed samples were added to the MALDI target plate, dried in air, and then entered into mass spectrometry analysis. The mass spectrometry con...

Embodiment 3

[0066] Example 3 enrichment and detection of serine and phenylalanine

[0067] Solutions containing different small molecules were subjected to solid-phase microextraction and mass spectrometry using matrix solutions containing hexagonal boron nitride nanosheets.

[0068] Serine (ser) and phenylalanine (phe) were prepared into solutions with a concentration of 5 μmol / L, respectively, and 495 μL (mixed with ser solution) and 5 μL (mixed with phe solution) of 0.25 mg / mL h-BN nanoparticles were taken respectively. Tablet suspension mix. After incubating in a shaker for 15 min, centrifuge (10,000 g), resuspend the pellet into 10 μL of methanol aqueous solution with a volume ratio of 1:1, and take 1 μL of the spot target. Perform mass spectrometry tests. The mass spectrometry conditions are: voltage: accelerating voltage: 19.000kv; delay extraction voltage: 14.920kv; reflector voltage: 20.000kv; lens voltage: 7.000kv; frequency: 1.000Hz; laser energy: 75-80%; ; Negative ion mode...

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Abstract

The invention provides application of a hexagonal boron nitride nanosheet in preparing a matrix solution and a mass spectrometric detection method, and particularly provides application of a hexagonal boron nitride naonsheet in preparing a matrix solution. The matrix solution is used for matrix assistant laser desorption/ionization mass spectrometric detection. Therefore, a matrix can be prevented from generating background peaks in the analysis process not to interfere the detection of a sample, so that the accuracy of the mass spectrometric detection can be improved.

Description

technical field [0001] The present invention relates to the field of mass spectrometry, in particular, to a method for analyzing small molecules by matrix-assisted laser desorption / ionization mass spectrometry. Background technique [0002] Molecular weight is the most basic physical and chemical property parameter of organic compounds. Whether the molecular weight is correct or not often represents whether the structure of the measured organic compounds and biological macromolecules is correct or not. Matrix-assisted laser desorption / ionization mass spectrometry (MALDI-MS) is a new type of soft ionization biological mass spectrometry in recent years. The principle of matrix-assisted laser desorption / ionization (MALDI) is to irradiate the co-crystalline film formed by the matrix and sample molecules with laser light. The energy absorbed from the laser is transferred to the sample molecules, the protons are transferred to the sample molecules or the protons are obtained from...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/64
CPCG01N27/64
Inventor 聂宗秀孙洁王佳宁何清陈素明
Owner INST OF CHEM CHINESE ACAD OF SCI
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