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Method for distinguishing true apple juice from false apple juice by virtue of liquid chromatogram-isotope ratio mass spectrometry (LC-IRMS) determination

A technology of isotope mass spectrometry and isotope mass spectrometer, applied in the field of fruit juice, can solve the problem of unseen real and fake apple juice, and achieve the effect of high sensitivity, huge economic and social benefits, and damage prevention.

Inactive Publication Date: 2014-12-17
祝伟霞
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But so far there is no public report on the method of using liquid chromatography-isotope mass spectrometry to determine the identification of true and false apple juice

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  • Method for distinguishing true apple juice from false apple juice by virtue of liquid chromatogram-isotope ratio mass spectrometry (LC-IRMS) determination
  • Method for distinguishing true apple juice from false apple juice by virtue of liquid chromatogram-isotope ratio mass spectrometry (LC-IRMS) determination
  • Method for distinguishing true apple juice from false apple juice by virtue of liquid chromatogram-isotope ratio mass spectrometry (LC-IRMS) determination

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Embodiment Construction

[0024] The specific implementation of the present invention will be described in detail below in combination with specific situations.

[0025] When the present invention is implemented, it is realized by the following steps:

[0026] 1. Juice preparation

[0027] Preparation of natural apple juice: Take 1-1.5kg of 234 kinds of apples from different origins, put them in a juicer to squeeze the juice, centrifuge, collect the supernatant, and make 234 kinds of sample liquids, and store them in a freezer at -18°C to prevent Oxidation, deterioration;

[0028] 2. Analyze the plant sugar in the prepared apple juice, establish the plant sugar measurement conditions, and the plant sugar includes fructose, glucose and sucrose, and the method is:

[0029] A, to apple juice pretreatment, the 234 kinds of natural apple juice sample liquids prepared are respectively got 0.3g and add water to constant volume 50mL, then get 200 μ L and add water and dilute to 1mL, measure with liquid chrom...

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Abstract

The invention relates to a method for distinguishing true apple juice from false apple juice by virtue of liquid chromatogram- isotope ratio mass spectrometry (LC-IRMS) determination. The method can be used for effectively distinguishing true apple juice from false apple juice and ensuring the quality safety of apple juice products. The method comprises the following steps: respectively preparing apples from different places into apple juice samples; analyzing plant polysaccharides in the prepared apple juice samples to establish plant polysaccharide determination conditions, and analyzing malic acid in the apple juice to establish the plant polysaccharide determination conditions; and analyzing natural apple juice to establish a linear relationship between the delta <13>C ratio of plant polysaccharides and malic acid in the apple juice to make judgment on the true and false apple juice, wherein if the delta <13>Csuc (sucrose) ranges from -1.909% to -2.647%, the delta <13>Cglu (glucose) ranges from -2.452% to -3.050%, the delta <13>Cfru (fructose) ranges from 2.522% to 3.196%, the ratio delta 1 of plant polysaccharides ranges from 0.27 to 2.78, the delta <13>Cmal (malic acid) ranges from -2.531% to 3.257% and the linear dependence coefficient R2 is more than 0.7255, the apple juice is true. The method is accurate in determination results and high in sensitivity, the quality of apple juice can be effectively guaranteed, adulterated apple juice can be prevented from causing injury to consumers, and economic and social benefits are tremendous.

Description

technical field [0001] The invention relates to fruit juice, in particular to a method for identifying true and false apple juice by using liquid chromatography-isotope mass spectrometry. Background technique [0002] Because of its high nutritional value and easy absorption and utilization, apple juice is a favorite drink for consumers. As people's demand for natural fruit juices is increasing, various natural or processed fruit juices have emerged. my country's apple juice is rich in resources and various in variety. It occupies an important position in the gross national product and is also an important product for export. In order to ensure the quality of apple juice and the smooth export of products, it is necessary to improve the quality of apple juice products first, improve the quality inspection of apple juice in my country, and grasp the quality of apple juice from the source of processing. At present, there are many kinds of apple juices on the market and their ...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88
Inventor 祝伟霞杨冀州袁萍刘亚风刘胜男魏蔚杨娜
Owner 祝伟霞
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