The invention discloses essential oil for cleaning and caring female private parts and a preparation method of the essential oil, aiming at the problems that the existing products are complicated and inconvenient to use, and not obvious in effect and long in treatment cycle, easily cause injuries or secondary infection of the private parts, and have abundant using contraindication, and consumers can have serious problems due to improper use. The essential oil is prepared from the following components in percentage by weight: 10-15% of lavender spike essential oil, 17-22% of palmarosa essential oil, 12-20% of tea tree essential oil, 18-23% of clary sage essential oil, 10-15% of leptospermum scoparium essential oil, 10-15% of eucalyptus essential oil, and 4-9% of thujanol thyme essential oil. By utilizing the characteristics that the plant essential oil has the excellent antibacterial, antiviral, antimicrobic, sterilization and inflammation-diminishing efficacies, the principle that the plant essential oil has the volatility, the characteristic that the plant essential oil with complicated components can not have drug resistance like antibiotics, as well as the characteristic that that the essential oil has more curative effects through scientific mixing, the essential oil provided by the invention has the advantages of obvious effect and rapidness in taking effect.