92results about "Particle spectrometer methods" patented technology

And re-sampling triggered by uncertain results in mass spectrum screening. The invention provides a system / technical scheme for promoting repeated sample injection triggered by uncertain results in mass spectrum screening. In various embodiments, the system may access a screening list specifying a plurality of target compounds. In aspects, the system can classify a plurality of target compounds as present or absent in a sample, respectively, based on causing a chromatograph and a mass spectrometer to perform an iterative injection scheme on the sample, with subsequent iterations of the iterative injection scheme having progressive compound selectivity, and is triggered by the uncertain presence or absence of the classification result in the previous iteration.

A system includes a first pre-separation device configured to perform a first pre-separation of precursor ions according to mobilities of the precursor ions and a second pre-separation device positioned downstream of the first pre-separation device configured to perform a second pre-separation of precursor ions based on a mass-to-charge ratio (m / z) of the precursor ions. The system further includes a mass spectrometer positioned downstream of the second pre-separation device configured to acquire mass spectra for precursor ions emitted from the second pre-separation device. The second pre-separation device is synchronized with the mass spectrometer such that an m / z range of precursor ions emitted from the second pre-separation device corresponds to a precursor m / z isolation window of the mass spectrometer.

Systems and methods are disclosed for automated method parameter configuration for differential mobility separations. As non-limiting examples, various aspects of this disclosure provide receiving a sample in an open port interface; transferring the sample to an ionization source; ionizing the transferred sample; introducing the ionized sample into a mass spectrometer; mass analyzing the ionized sample to produce an initial mass analysis result; determining a peak width of the initial mass analysis result; and determining a dwell time for subsequent measurements based on the determined peak width, a pre-defined number of data points across subsequent mass analysis peak widths, and a number of different analytes to be assessed for the sample. The sample may be diluted and transferred to the ionization source by a sample introduction apparatus selected from a group including an acoustic droplet ejector (ADE), a pneumatic ejector, a piezoelectric ejector, and a hydraulic ejector.

The invention relates to the quantitative measurement of steroidal compounds by mass spectrometry. In a particular aspect, the invention relates to methods for quantitative measurement of steroidal compounds from multiple samples by mass spectrometry.

Disclosed herein are methods and systems for individual ion mass spectrometry (I2MS). The methods and systems described herein parse I2MS data and allows for informed tandem mass spectrometry analyses by determining a target m / z that increases the yield of an intact mass corresponding to an analyte of interest while reducing the yield of additional detectable masses.

A method for optimizing at least one parameter setting of at least one mass spectrometry device (110) operating at unit resolution is disclosed. The method comprises the following steps:a) determining at least one analyte detection window for detecting an analyte of interest with the mass spectrometry device (110), wherein the analyte detection window is defined by a central mass to charge ratio value of the analyte and a predefined width, wherein the central mass to charge ratio value of the analyte is set to a theoretical mass to charge ratio value of the analyte of interest having more than one decimal place and / or a mass to charge ratio value of the analyte of interest determined by a high resolution mass spectrometry measurement having more than one decimal place;b) determining at least one internal standard detection window for detecting an internal standard substance with the mass spectrometry device (110), wherein the internal standard detection window is defined by a central mass to charge ratio value of the internal standard substance and the pre-defined width, wherein the central mass to charge ratio value of the internal standard substance is set to a mass to charge ratio value of the internal standard substance calculated relative to the analyte of interest and having more than one decimal place and / or to a mass to charge ratio value of the internal standard substance determined by a high resolution mass spectrometry measurement having more than one decimal place.

A miniature electrode apparatus is disclosed for trapping charged particles, the apparatus includes, along a longitudinal direction, a first end cap electrode, a central electrode having an aperture, and a second end cap electrode. The aperture is elongated in the lateral plane and extends through the central electrode along the longitudinal direction and the central electrode surrounds the aperture in a lateral plane perpendicular to the longitudinal direction to define a transverse cavity for trapping charged particles. Electric fields can be applied in a y-direction of the lateral plane across one or more planes perpendicular to the longitudinal axis to translocate and / or manipulate ion trajectories.

A method for multiple reaction monitoring using a mass spectrometer (106) is proposed. The method comprises i) measuring (128), using a mass spectrometer (106), multiple reaction monitoring transitions of both a quantifier and a qualifier of both an internal standard and an analyte using alternating multiple reaction monitoring, the alternating multiple reaction monitoring comprising at least three multiple reaction monitoring channel groups, one of the multiple reaction monitoring channel groups being measured at the respective theoretical m / z values of both the quantifier and the qualifier of both the internal standard and the analyte, and the two other multiple reaction monitoring channel groups being measured at respective m / z values shifted by a predetermined level higher and lower; ii) comparing (130), for at least two of the groups, at least two of the quantifier / qualifier ratios of the multiple reaction monitoring transitions of the internal standard with reference values from a database (126) using at least one processing device (120), the comparing including determining a deviation between the quantifier / qualifier ratio and the reference value; iii) determining (132), if at least one deviation of the quantifier / qualifier ratio is within at least one predetermined tolerance range, using a processing device (120), a measurement result from the measured multiple reaction monitoring transitions of the analyte and the internal standard, and, if not, rejecting (136) the measured multiple reaction monitoring transitions; and including.

A system includes a first pre-separation device configured to perform a first pre-separation of precursor ions according to mobilities of the precursor ions and a second pre-separation device positioned downstream of the first pre-separation device configured to perform a second pre-separation of precursor ions based on a mass-to-charge ratio (m / z) of the precursor ions. The system further includes a mass spectrometer positioned downstream of the second pre-separation device configured to acquire mass spectra for precursor ions emitted from the second pre-separation device. The second pre-separation device is synchronized with the mass spectrometer such that an m / z range of precursor ions emitted from the second pre-separation device corresponds to a precursor m / z isolation window of the mass spectrometer.

Disclosed herein are scientific instrument support systems, as well as related methods, computing devices, and computer-readable media. For example, in some embodiments, an instrument includes a sample introduction device and a mass spectrometer. The mass spectrometer includes first logic for receiving a sample containing an analyte from a queue, second logic for determining instrument methods and data analysis parameters based on a property of the analyte, and third logic for processing the sample by applying the instrument methods and data analysis parameters.

An ion source is disclosed that comprises a positioning assembly that can position a sprayer with respect to an ion inlet. The ion source includes a first imaging device that can provide a first view of a position of the sprayer with respect to the ion inlet, and a second imaging device that can provide a second, different view of a position of the sprayer with respect to the ion inlet. The second view has a higher magnification than the first view.

A sampling interface enables the sampling of ions in a mass spectrometer for subsequent spectroscopic analysis. The sampling interface comprises: an inlet receiving a quantity of particles from an ion source, the quantity of particles including ions for spectroscopic analysis; a skimmer downstream of the inlet and comprising an aperture through which particles from the inlet pass; an extraction lens downstream of the skimmer and configured for extracting ions from the particles passed through the aperture at the skimmer; an adjustable voltage source applying a bias voltage to the extraction lens so as to generate an electric field in at least a region between the extraction lens and the skimmer; and a controller controlling the adjustable voltage source to apply a bias voltage to the extraction lens, in order to control the reduced electric field in the region immediately downstream of the skimmer.

Methods for acquiring mass spectral data of a sample across at least a portion of an m / z range include receiving mass spectral data of the sample across the m / z range. The m / z range is partitioned into one or more sets of m / z sub-ranges, each set comprising one or more m / z sub-ranges, by dividing the m / z range into a plurality of m / z bins, determining an indication of ion abundance for each m / z bin, based on the mass spectral data, and forming an m / z sub-range of the one or more sets of m / z sub-ranges by assigning m / z bins having ion abundances that correspond to at least a threshold degree to the formed m / z sub-range. A mass analysis is performed on the sample for each set of m / z sub-ranges, thereby acquiring one or more partial mass spectral data sets.

The invention relates to an imaging elemental analyzer for imaging one or more analyte elements in an organic sample, wherein the analyzer comprises: a chamber for receiving an organic sample containing one or more analyte elements to be imaged, wherein the internal chamber pressure surrounding the sample is in the range of 10 -5 up to 10 -2mbar; at least one irradiant selected from: (i) an ion gun for irradiating the sample with a high-intensity primary ion beam, wherein the primary ions are generated in the ion gun at a pressure below 1 mbar, the ion gun serving to focus the primary ion beam onto a localized spot on the sample surface and to move the spot over time to a plurality of locations on the sample surface; (ii) a laser for irradiating a localized spot on the sample surface and to move the spot over time to a plurality of locations on the sample surface; a gas-filled RF ion guide for receiving the generated ions, comprising the elements released from the sample in response to irradiation by the primary ions or the laser, wherein the RF ion guide prevents the propagation of any ions whose m / z values ​​are less than the mass or mass range of the analyte elements;and a time-of-flight (TOF) mass analyzer for recording the ions or reaction products of the secondary ions formed from the RF ion guidance, wherein the TOF mass analyzer is configured for a repetition rate of at least 5 kHz. The invention also relates to a corresponding method and a corresponding device for imaging one or more inorganic, non-volatile elements.

The present invention relates to systems and methods for inductively coupled plasma (ICP) analysis, including mass cytometry. Inductively coupled plasma (ICP) analyzers use an ICP torch to generate a plasma that atomizes and ionizes a sample. Analysis of the atomic ions can be performed by atomic analysis, such as mass spectrometry (MS) or atomic emission spectrometry (AES). Particle-based ICP analysis includes analysis of particles, such as cells, beads, or laser ablation plumes, by atomizing and ionizing the particles in an ICP torch prior to atomic analysis. In mass cytometry, the mass tags of the particles are analyzed by mass spectrometry, such as ICP-MS. The systems and methods of the present application include one or more of an ICP load coil including annular fins, particle suspension sample introduction fluidics, and an ICP analyzer.

To provide a system for performing multiplexed ion pre-separation for mass spectrometry.SOLUTION: The system includes a pre-separation device for separating the precursor ions into a set of discrete fractions of precursor ions based on a physical property of the precursor ions and for sequentially transmitting a first subset of the discrete fractions of precursor ions included in the set of discrete fractions of precursor ions. The system further includes a mass spectrometer positioned downstream of the pre-separation device for receiving the first subset of the discrete fraction of precursor ions. The mass spectrometer includes an ion storage for accumulating a first population of product ions generated from each discrete fraction of precursor ions included in the first subset of discrete fractions of precursor ions and a mass analyzer for performing mass analysis of the first population of product ions.SELECTED DRAWING: Figure 1

The technical problem of the present application is to rapidly stabilize lactone in a sugar chain. A sample preparation method is a sample preparation method for preparing a sample containing a sugar chain, the preparation method including the steps of: performing a lactonization reaction that lactonizes at least a portion of sialic acid contained in the sugar chain; and performing an amidation reaction that amidates the lactone of the lactonized sialic acid by adding an amidation reaction solution to the sample, the amidation reaction solution containing at least one selected from the group consisting of ammonia, amines, and salts thereof that react with the lactonized sialic acid.

A method of mass spectrometry and / or mobility spectrometry includes: providing an ion separation device including a plurality of electrodes; providing a first force to urge the ions in a first direction along an axis of the ion separation apparatus while also providing a second force to urge the ions in a second, opposite direction such that ions of different mobility or mass-to-charge ratios reach different equilibrium positions at different locations along the axis; and varying the first force and / or the second force during a single elution cycle of the ion separation device so as to sequentially release ions from the ion separation device in an increase order of ion mobility or mass-to-charge ratio or in a decrease order of ion mobility or mass-to-charge ratio; wherein the altering step is performed such that, during the elution cycle, ions in a first range of mobility or mass-to-charge ratio are eluted from the ion separation device at a first rate and ions in a second, separate range of mobility or mass-to-charge ratio are eluted from the ion separation device at a second, different rate.

A method for optimizing at least one parameter setting of at least one mass analyzer (110) operating at unit resolution is disclosed. The method includes: a) determining at least one analyte detection window for detecting an analyte of interest using a mass analyzer (110), the analyte detection window being defined by a central mass-to-charge ratio value of the analyte and a predefined width, the central mass-to-charge ratio value of the analyte being set to a theoretical mass-to-charge ratio value of the analyte of interest having two or fewer decimal places and / or a mass-to-charge ratio value of the analyte of interest determined by a high-resolution mass spectrometric measurement having two or fewer decimal places; and b) determining at least one internal standard detection window for detecting an internal standard using the mass analyzer (110), the internal standard detection window being defined by a central mass-to-charge ratio value of the internal standard and a predefined width, the central mass-to-charge ratio value of the internal standard being set to a mass-to-charge ratio value of the internal standard calculated for the analyte of interest having two or fewer decimal places and / or a mass-to-charge ratio value of the internal standard determined by a high-resolution mass spectrometric measurement having two or fewer decimal places.

A business method for use in classifying patient samples. The method includes steps of collecting case samples representing a clinical phenotypic state and control samples representing patients without said clinical phenotypic state. Preferably the system uses a mass spectrometry platform system to identify patterns of polypeptides in said case samples and in the control samples without regard to the specific identity of at least some of said polypeptides. Based on identified representative patterns of the state, the business method provides for the marketing of diagnostic products using representative patterns. The present invention relates to systems and methods for identifying new markers, diagnosing patients with a biological state of interest, and marketing / commercializing such diagnostics. The present invention relates to systems and methods of greater sensitivity, specificity, and / or cost effectiveness.

A method for determining the presence of a perfluoro- or polyfluorochemical (PFC), such as a PFAS compound, in a sample is described. The method involves generating sample ions from the sample using electron impact ionization (El), direct laser ionization, or field desorption ionization, and mass-analyzing the sample ions using a mass analyzer to generate mass spectral data. The mass analyzer has a mass accuracy of approximately ≤ 100 ppm and a resolution of approximately ≥ 10,000. The method further includes determining whether an ion peak is present in the mass spectral data exhibiting an accurate mass-to-charge ratio within ± 100 ppm of a first accurate mass-to-charge ratio (m / z). The first accurate mass-to-charge ratio (m / z) is the exact mass-to-charge ratio of an ion that indicates the presence of a PFC in the sample.The procedure further includes determining whether a PFC is present in the sample if it is determined that an ion peak is present which has an accurate mass-to-charge ratio within ± 100 ppm of the first accurate mass-to-charge ratio m / z.

The invention relates to the quantitative measurement of steroidal compounds by mass spectrometry. In a particular aspect, the invention relates to methods for quantitative measurement of steroidal compounds from multiple samples by mass spectrometry.

Methods for operating a mass spectrometer, particularly an inductively coupled plasma mass spectrometer (ICP-MS), having a skimmer 30 and a circuit 40 for applying an electric potential to the skimmer

A charge detection mass spectrometer (CDMS) includes an electrostatic linear ion trap (ELIT), a processor, and a memory storing instructions executable by the processor to (a) control the ELIT to trap ions, (b) collect ion measurement information as the trapped ions oscillate back and forth through the ELIT, the ion measurement information including the charge induced by the ions on the charge detector of the ELIT during each passage of the ions through the ELIT and the timing of the induced charges relative to each other, (c) process the ion measurement information in the time domain of each of a plurality of sequential time windows to determine the charge magnitude of the ions during each time window, and (d) determine the charge magnitude of the trapped ions based on the charge magnitude value of each time window.

The invention relates to a method and an apparatus for analyzing a sample material on a sample carrier, comprising the following modes of operation: providing a time-of-flight mass analyzer having an ion generating unit, an ion generation unit having a mount for the sample carrier, an ion receiver, a flight path defining a longest flight time therebetween, an ion selector disposed along the path, and a clock generator for repeatedly triggering an ion generation pulse at the sample carrier and a subsequent pulse for accelerating ion species into the flight path; defining one or more mass-to-charge ratio (m / z) ranges, each range upper limit corresponding to a flight time shorter than the longest flight time; selecting the cycle period of the ion generation pulse so that the continuous pulse interval is shorter than the longest flight time but longer than the acceleration time; and using the mass analyzer, selecting a pulse cycle mode and an ion selector to analyze the sample material.

A method and a system for self-regulating control of an internal chamber pressure of a mass spectrometer are provided. The method specifically includes: associating a first test mass spectrum set, a sample composition, a compositional ratio, and a chamber pressure regulation parameter to obtain a test data set; performing characteristic factor identification on a first real-time mass spectrum generated in real time to obtain a plurality of first real-time characteristic factors when a mass spectrometer is used for sample detection; matching the first real-time characteristic factors with different test data sets; and regulating an internal chamber pressure of the mass spectrometer according to the chamber pressure regulation parameter in the test data set when there exists a test data set with a matching degree greater than or equal to a preset value.

A method of analysing a field of a mass spectrometer comprising a mass analyser and a static field mass filter having a first Wien filter and a second Wien filter is provided. The method includes, for each of a plurality of predetermined strengths of one of an electric field or a magnetic field of the first and second Wien filters: setting the one of the electric field or the magnetic field of the first and second Wien filters to the predetermined strength; causing a beam of ions comprising one or more ion species to be injected through the static field mass filter; and measuring, using the mass analyser, a respective intensity of ions of each of the one or more ion species in the beam.

This invention describes techniques and apparatus for determining the atomic-level configuration of product ions. In some embodiments, product ion CCS information can be used to determine the substructural configuration of the product ion, such as isomers. For example, in one embodiment, the apparatus may include at least one memory and logic coupled to the at least one memory to: receive resolution information of a plurality of product ions, the resolution information including product ion collision cross-section (CCS) information; and for at least one of the plurality of product ions, determine the variance value of the product ion CCS information. Other embodiments are described in this invention.

A method of determining a minimum concentration of a first analyte to be mass analysed in a mass spectrometer, comprising: (i) mass analysing a known concentration of said first analyte according to a