20 results about "Protoporphyrin IX" patented technology

The invention belongs to the technical field of gas sensors, and discloses a portable ratio fluorescent film sensor for visual detection of oxygen as well as a preparation method and application of the portable ratio fluorescent film sensor. According to the sensor, polymethyl methacrylate is used as a base material, a carbon dot emitting blue light is used as a fluorescent indicator, protoporphyrin emitting red light is used as a fluorescent reference agent, and the two materials are physically blended to form a blue-red dual-emission system; under the excitation of 365nm ultraviolet light, the oxygen concentration affects the yield of active oxygen, the active oxygen quenches the fluorescence of the carbon dots, the fluorescence of protoporphyrin is stable, and the sensor gradually changes from blue violet to red. Meanwhile, the invention discloses an oxygen quantitative / semi-quantitative detection method of the sensor. The preparation process is simple, the cost is low, the detection operation is simple and convenient, the anti-interference performance is strong, the high-sensitivity visual portable detection of the oxygen concentration can be realized, and the sensor is suitable for the fields of food packaging, medical monitoring, industrial safety and the like, and has the potential of large-scale production and commercial application.

The invention discloses a detection kit based on orthogonal dual-channel label-free CRISPR-Cas and application of the detection kit, relates to the technical field of nucleic acid detection, and constructs an orthogonal dual-channel response system by utilizing the differentiated cleavage activity of Cas12a targeted DNA and Cas13 targeted RNA to a substrate. A Cas12a pathway is reported by utilizing protoporphyrin IX to be compounded with G-quadruplex G4DNA, and a Cas13a pathway is reported by utilizing DFHBI to be compounded with a Broccoli RNA structure, so that label-free signal transduction is realized. Through accurate screening of fluorophores, channel specificity is ensured, and optical signal crosstalk is eliminated. Mycobacterium tuberculosis (MTB) and respiratory syncytial virus (RSV) are used as model pathogens for verification, and a multiple recombinase polymerase amplification (RPA) technology is combined, so that the detection sensitivity on a synthetic target reaches a single molecule level. The system has high specificity, and even if the concentration of a non-target pathogen is increased by 100 times, no cross reaction exists.

The present invention relates to a fluorescence observation system, to a method for carrying out a fluorescence observation and to a filter set that can be used for this purpose for simultaneously observing fluorescent light of protoporphyrin IX in an object and non-fluorescent regions of the object in an almost true-colour representation. The filter set comprises an illumination filter and an observation filter. A transmittance of the illumination filter has an average value of at least 90% from 405 nm to 452 nm, is at most 0.01% in at least 95% of a wavelength range extending from 466 nm to 524 nm, has an average value of at least 0.028% and at most 0.044% from 535 nm to 595 nm, and is at most 0.003% in at least 95% of a wavelength range extending from 610 nm to 750 nm. A transmittance of the observation filter has an average value of at least 0.143% and at most 0.187% from 404 nm to 438 nm, is at most 0.01% in at least 95% of a wavelength range extending from 444 nm to 534 nm, and has an average value of at least 90% from 544 nm to 722 nm.

A method to make Co- or Ni-substituted protoporphyrin IX by incubating an E. coli strain in a rich medium containing iron ions and added Co2+ or Ni2+ ions, for a time and under conditions wherein the E. coli strain produces Co- or Ni-substituted protoporphyrin IX. The E. coli host may be transformed to contain and express a gene construct encoding a mutant EcHemH including a L13R mutation.

The invention belongs to the technical field of biological medicine, and discloses a prodigiosin (PG) and tannic acid (TA) self-assembled carrier-free nano-particle as well as preparation and application thereof. The particles are formed by PG and TA through nano-precipitation self-assembly, and the particle size is 30-50 nm. The preparation method comprises the following steps: respectively dissolving PG and TA in DMSO, mixing and stirring according to a molar ratio of (5-10): 1, dropwise adding ddH2O, carrying out ultrasonic treatment, dialyzing to remove a solvent, centrifuging, taking precipitate, and freeze-drying to obtain powder. The nanoparticles can efficiently kill methicillin-resistant staphylococcus aureus and other multi-drug-resistant bacteria, remove excessive ROS, inhibit TNF-alpha, IL-1beta and IL-6 and promote expression of IL-4, IL-10 and TGF-beta, so that infected skin flap necrosis is relieved, skin repair is promoted, and the nanoparticles have good biological safety and can be used for preparing antibacterial and infected wound treatment drugs.

The application belongs to the technical field of biomedicine, and particularly relates to a CuTA-Por@epsilon-PL nano platform and a preparation method and application thereof. The nano platform is prepared by grafting protoporphyrin (Por) through amidation reaction of CuTA, and wrapping the CuTA by epsilon-polylysine (epsilon-PL) through electrostatic adsorption, so as to synthesize CuTA-Por@epsilon-PL NPs (CPP NPs), which can be used as a potential treatment method for treating peri-implantitis, destroying bacterial biofilm and regulating inflammation.

The invention relates to a method and a sensor for detecting and / or quantifying a set of hematological parameters in a blood or derived biological fluid sample, the set of hematological parameters comprises at least one parameter selected from the group consisting of the number (RC) of a blood cell component in the blood or derived biological fluid, the average blood cell volume (MCV) of said blood cell component, the percentage (HCT) of the blood cell component in the sample volume and the amount of at least one substance (Hb) comprising a protoporphyrin metal complex. The invention also relates to a method for producing the electrical analysis sensor.

The invention belongs to the technical field of biological pharmacy, and particularly discloses an application of protoporphyrin IX in preparation of an anti-akabane virus drug. By measuring the safe concentration range of the protoporphyrin IX on MDBK cells and through RT-qPCR, WB, IFA and TCID50, it is found that the protoporphyrin IX can serve as an inhibitor of AKAV viruses, when the concentration of the protoporphyrin IX is 10 [mu] M, the protoporphyrin IX has a remarkable inhibition effect on the AKAV viruses, and the application of the protoporphyrin IX in preparation of the anti-akabane virus drug in preparation of the anti-akabane virus drug in preparation of the anti-akabane virus drug in preparation of the anti-akabane virus drug is achieved. And a new candidate medicine is provided for preventing and treating AKAV virus infection.

This invention provides an in-situ gel delivery carrier for 5-aminolevulinic acid and its preparation method, belonging to the field of biomedical technology. The in-situ gel delivery carrier comprises TAT ​​peptide and *Rhizopus oryzae* lipase; the mass-to-volume ratio of TAT peptide to *Rhizopus oryzae* lipase is 0.03-0.07 g: 0.005-0.015 g. This in-situ gel delivery carrier combines a dual mechanism of "physical thermosensitive phase transition" and "biological thermosensitive enzyme activity" to achieve long-term, targeted adhesion to lesion sites. Through the nonlinear synergistic effect of *Rhizopus oryzae* lipase and TAT peptide, it precisely degrades lipid barriers and efficiently delivers drugs, thereby significantly reducing the drug concentration while exponentially increasing the conversion efficiency of protoporphyrin IX (PpIX) in target cells. The preparation method is simple to operate.

The present invention relates to a fluorescence observation system, a method for performing fluorescence observation, and a filter set usable therefor for the simultaneous observation of fluorescent light from protoporphyrin IX in an object and of non-fluorescent areas of the object in a nearly color-accurate representation. The filter set comprises an illumination filter and an observation filter. The transmittance of the illumination filter has an average value of at least 90% from 405 nm to 452 nm, is at most 0.01% in at least 95% of a wavelength range extending from 466 nm to 524 nm, has an average value of at least 0.028% and at most 0.044% from 535 nm to 595 nm, and is at most 0.003% in at least 95% of a wavelength range extending from 610 nm to 750 nm.The transmittance of the observation filter has an average value of at least 0.143% and at most 0.187% from 404 nm to 438 nm, is at most 0.01% in at least 95% of a wavelength range extending from 444 nm to 534 nm, and has an average value of at least 90% from 544 nm to 722 nm.

This invention relates to a compound and its preparation method, a modular peptide nanovaccine and its preparation method and application, and relates to the pharmaceutical field. The chemical structural formula of the compound is shown in Formula I. This invention provides a compound containing an mPEG-PLA structure, a protoporphyrin structure chelated with cobalt ions, and a β-cyclodextrin structure, endowing it with excellent binding ability to peptides and excellent loading capacity with adjuvants, achieving co-delivery of peptides and adjuvants. Based on this, this invention also provides a modular peptide nanovaccine with high peptide loading rate and "plug-and-play" characteristics. It can significantly inhibit tumor growth in a B16 subcutaneous tumor model, providing an efficient and flexible technical platform for the prevention of melanoma and other tumors and the clinical translation of peptide vaccines, with broad application prospects.

The invention discloses a multifunctional feed additive for improving yolk chromaticity and whole egg quality, and belongs to the technical field of feed additives. The additive is prepared by mixing the following dry substances in parts by weight: 300-500 parts of pine needle meal, 100-150 parts of hermetia illucens powder, 2-8 parts of cantharides yellow, 0.5-5 parts of protoporphyrin and 0.3-1 part of vitamin E. The feed additive disclosed by the invention is added into daily ration of laying hens at the dosage of 1-5%, so that the chromaticity of egg yolk can reach 11-12 grades, the egg yolk is still effective after 10 days after the addition is stopped, the feed additive is obviously superior to an existing single or binary compound colorant, and meanwhile, the egg shell strength is improved, the Hough unit is increased, and the problem of quality decline of eggs laid by the laying hens in the later period of egg laying is solved. The technical problems that an existing egg yolk coloring agent is single in function, short in coloring effect lasting time and incomplete in egg quality improvement are solved, and the egg yolk coloring agent has safety, economical efficiency and practicability.

A method for performing photodynamic therapy is provided. The method includes applying a topical composition to the skin of a patient. The topical composition includes 5-aminolevulinic acid (ALA) hydrochloride and a vehicle including at least one chelating agent for enhancing accumulation of protoporphyrin IX (PpIX) in the skin. The method further includes incubating the topical composition and, after incubation, applying heat from a heat source to the skin for at least a first period of time.

While clinical treatment of actinic keratosis by photodynamic therapy (PDT) is widely practiced, there is well-known variability in response, primarily caused by heterogeneous accumulation and PDT-induced photobleaching of the photosensitizer protoporphyrin IX (PpIX) between patients and between lesions. One of the key factors in regularizing this treatment would be to have an easily accessible indicator of PpIX present in the lesions at the time of light delivery. Described herein, a smartphone-based fluorescence imager was developed to allow simple quantitative photography of the lesions and their PpIX levels.

Provided are methods of isolating or extracting human mitochondrial tryptophan-rich sensory protein (HsTSPOl). The methods include contacting the H.sTSPOl with an effective amount of at least one Native Cell Membrane Nanoparticle (NCMN) polymer and recovering the TSPO. Compositions containing TPSO encapsulated in a particle formed by the NCNM polymer are also provided. A protoporphyrin derivative compound, methods of producing bilindigin or derivatives thereof, and methods of screening compounds that modulate TSPO activity are also provided.

A composition comprising Protoporphyrin IX (PpIX) or a pharmaceutically acceptable salt thereof and a pharmaceutical excipient or a TauT inhibitor or a pharmaceutically acceptable salt thereof, and a pharmaceutically acceptable excipient, wherein preferably the excipient is a carrier or a diluent.

The invention relates to the technical field of medicine application, in particular to application of protoporphyrin IX in medicine for inhibiting porcine reproductive and respiratory syndrome virus. The PpIX has a remarkable antiviral effect on porcine reproductive and respiratory syndrome virus infection, can be used for preparing anti-PRRSV drugs, provides a reliable basis for treatment and prevention and control of diseases caused by the virus, and provides a new thought and an effective coping strategy for prevention and control work of PRRSV. The research also discovers the optimal action mode of PpIX for inhibiting PRRSV proliferation and the period of PRRSV replication, and a theoretical basis is provided for prevention and control of PRRS.

Certain embodiments of the invention provide a method of lowering Protoporphyrin IX (PP-IX) level or treating protoporphyria (e.g., EPP) in a subject in need of, comprising administering one or more antihistamines (e.g., administering an H1 antihistamine, or administering two antihistamines such as an H1 antihistamine and an H2 antihistamine) to the subject.

The invention relates to the technical field of biological medicines, in particular to application of tryptophan in preparation of a laying hen feed additive for preventing or reducing freckle eggs. According to the application, relevance deep study is carried out between the action mechanism of tryptophan in synthesis of protoporphyrin-IX pigment in reproductive organs of freckle laying hens and generation of freckle eggs, and it is found that tryptophan can influence synthesis of protoporphyrin-IX pigment by regulating ALAS1 gene expression in the reproductive organs of the freckle laying hens; therefore, freckle egg formation is prevented or reduced, and eggshell freckles are reduced.

The invention discloses a microneedle patch based on a flexible QLED light source and a preparation method and application of the microneedle patch, and belongs to the technical field of medical instruments. The micro-needle patch comprises a degradable hyaluronic acid micro-needle layer, a QLED light source layer, a flexible printed circuit and a substrate layer, the degradable hyaluronic acid micro-needle layer jointly carries iron-platinum nano-enzyme (FePt NPs) and photosensitizer protoporphyrin (PpIX), and the degradable hyaluronic acid micro-needle layer achieves transdermal targeted delivery of the photosensitizer protoporphyrin and the iron-platinum nano-enzyme in a micro-needle array mode. The QLED light source layer emits exciting light with a specific wavelength to activate the photosensitizer in situ to generate toxic singlet oxygen, and in an acidic microenvironment of melanoma cells, the iron-platinum nano-enzyme can trigger a cascade reaction to consume an antioxidant glutathione in the cells in cooperation with the iron-platinum nano-enzyme, so that the toxic singlet oxygen is generated. Lipid peroxidation accumulation is induced, so that melanoma cells are induced to generate apoptosis and ferroptosis, and the synergistic killing effect of photodynamic therapy and ferroptosis therapy is achieved.