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Methods for overproducing protoporphyrin ix in algae and compositions therefrom

一种组合物、原卟啉的技术，应用在单细胞藻类的蛋白质组成、生物化学设备和方法、微生物等方向，能够解决限制积极影响、经济不佳、吸引力小等问题

Pending Publication Date: 2020-09-11
TRITON ALGAE INNOVATIONS INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This approach still requires purification of the hemopexin from the expression host prior to its incorporation into the final product, which limits the potential positive impact due to economic constraints
In addition to the poor economics, the product is also genetically modified, making it less appealing to many consumers who choose to eat non-GMO foods

Method used

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  • Methods for overproducing protoporphyrin ix in algae and compositions therefrom
  • Methods for overproducing protoporphyrin ix in algae and compositions therefrom
  • Methods for overproducing protoporphyrin ix in algae and compositions therefrom

Examples

Experimental program
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Embodiment approach 1

[0091]Embodiment 1. A composition comprising a preparation from an algal strain, wherein the algal strain overexpresses or accumulates protoporphyrin IX (PPIX). 2. The composition of embodiment 2, the formulation being biomass from an algal strain. 3. The composition of embodiment 2, wherein the formulation is fractionated biomass from an algal strain. 4. The composition of embodiment 3, wherein the fractionated biomass comprises a PPIX-enriched fraction. 5. The composition of embodiment 4, wherein the PPIX-enriched fraction further comprises a protein-enriched fraction. 6. The composition of embodiment 1, wherein the preparation is an extracellular fraction of an algal culture. 7. The composition according to any one of embodiments 1-6, wherein the formulation is red or red-like in color. 8. The composition according to any one of embodiments 1-7, wherein the formulation contains a greater amount of PPIX than heme. 9. The composition of any one of embodiments 1-8, wherein...

Embodiment approach 27

[0092] Embodiment 27. A food product comprising the composition according to any one of embodiments 1-26. 28. The food product of embodiment 27, wherein the food product comprises clean meat, cultured meat, synthetic meat, plant-based meat, or non-animal cell-based meat. 29. The food product of embodiments 27-28, wherein said food product is selected from the group consisting of beef-like products, fish-like products, chicken-like products, pork-like products and meat replicas. 30. The food product of embodiments 27-29, wherein said food product is vegan, vegetarian or gluten-free.

Embodiment approach 31、 1

[0093] Embodiment 31. An edible ingredient comprising the composition according to any one of embodiments 1-25. 32. The edible ingredient of embodiment 31, wherein the ingredient is part of a finished product, wherein the finished product has a red or red-like color derived from the ingredient. 33. The edible ingredient of embodiment 31, wherein the ingredient is part of a finished product, wherein the finished product has a meat or meat-like flavor derived from the ingredient. 34. The edible ingredient of embodiment 31, wherein said ingredient is part of a finished product, wherein said finished product has a Hasan appearance derived from the blood of the ingredient. 35. The edible composition according to embodiments 31-34, wherein said finished product is an ingredient for burgers, sausages, kebabs, fillets, ground meat products or meatballs. 36. The edible ingredient according to embodiments 31-34, wherein said edible composition is part of a finished product, and wherein...

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Abstract

Provided herein are compositions and processes for producing compositions from an algae that overproduces protoporphyrin IX (PPIX). Also provided are methods of growing PPIX overproducing algae, methods of isolating PPIX-containing portions from algae cultures and compositions and methods of making food products with PPIX produced by algae. Provided herein are strains and methods to select strainsthat overproduce PPIX. Also provided are compositions, including edible compositions that include PPIX produced from algae.

Description

[0001] Cross References to Related Applications [0002] Pursuant to 35 U.S.C. §119(e), this application claims U.S. Provisional Application No. 62 / 865,800 filed June 24, 2019, U.S. Provisional Application No. 62 / 850,227 filed May 20, 2019, and 11, 2018 Priority to U.S. Provisional Application No. 62 / 757,534, filed March 8, the entire contents of which are incorporated herein by reference. [0003] sequence listing [0004] This application contains a Sequence Listing, which has been filed electronically in ASCII format and is hereby incorporated by reference in its entirety. Said ASCII copy, created on November 7, 2019, is named 20498-202380_SL.txt and is 208KB in size. Background technique [0005] Consumption of animal meat continues to grow with the advent of industrialized livestock farming. The livestock industry accounts for more than 18% of greenhouse gas production to date and is one of the leading causes of climate change. In addition to land use, livestock requi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23J3/20A23J3/22C07D207/34C07D487/22C07K14/795C12N1/12C12N15/113A23L27/26
CPCA23J3/227A23J3/20C12N15/1137A23V2200/26C12N2310/20C12Y499/01A23J1/009C12N9/93C12Y606/01001A23L13/428A23L17/60A23L5/46C12P17/182A23V2200/262A23K20/158A23K10/12A23K10/16C12N1/125A23V2002/00C12N9/88C12N15/11C12N2500/02C12N2500/24C12N2500/30C12N2500/34C12Y499/01001
Inventor 米勒·特朗约翰·迪顿布劳克·亚当斯迈克尔·玛菲尔德阿曼达·隆格奥斯卡·冈萨雷斯乔恩·汉森王迅大卫·施罗德
Owner TRITON ALGAE INNOVATIONS INC
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