63 results about "Deinococcus" patented technology

The present invention relates to a novel thermostable N-acylamino acid racemase (NAAAR) isolated from Deinococcus radiodurans NCHU1003, the coding sequence and the preparation theraof. The present invention also discloses the process for preparing highly optically pure L-amino acids, such as L-homophenylalanine (L-HPA) and the derivatives thereof, from N-protected amino acid by using the novel NAAAR combined with L-N-carbamoylase.

The present invention relates to ingredients for animal feed compositions for enhancing animal growth and / or animal health. The invention also relates to methods for producing such ingredients and feed compositions. The methods of the invention further allow improving the palatableness and / or digestibility of feed compositions. More specifically, the invention describes the use of a mix of Deinococcus or related bacteria and biomass as a supply of organic constituents in feed compositions.

The invention provides a production strain of high-yield keratin hydrolase and application of the strain, and particularly relates to anti-radiation deinococcus for producing keratin hydrolase. The strain expresses keratin hydrolase high in activity and wide in application range. The anti-radiation deinococcus can efficiently produce keratin hydrolase at low cost, and is very high in application value.

The invention finds that a lea-like gene (DR1172) in Deinococcus radiodurans R1 has the function of improving the adversity resistance of prokaryotes and plants. The invention constructs recombinant vectors containing the gene and the recombinant vectors are respectively transferred into prokaryotic and eukaryotic host cells. Experiments prove that the lea-like gene (DR1172) can improve the drought resistance of the rapes after being expressed in the prokaryotic host cell and the rapes.

The invention discovers that pprM gene in Deinococcus radiodurans R1 can enhance the drought resistance of prokaryotes and plants. Recombinant vectors comprising the gene are constructed and respectively introduced into procaryotic and enkaryotic host cells. Experiments prove that after the pprM gene is expressed in prokaryotic host cells and plants, the resistance to drought environment can be enhanced.

The invention relates to gene cloning of wholly new fucose synthetase and its expression in related host. The enzyme gene is cloned from Deinococcus radioduran, which can transform the malt to fucose under normal biochemical conditions. The substrate conversion rate rises while the reaction temperature reduces. The fucose can be used in food industry, sea product cold storage and pharmaceutical activity preserving.

The invention discloses an efficient lead ion bio-adsorbent and a preparation method and application thereof. The bio-adsorbent contains deinococcus radiodurans which is obtained from the American Type Culture Collection Center (ATCC) with a collection number of ATCC13939, the pH value of a Pb<2+>-containing solution to be treated is 2.0-4.0, and the concentration of the deinococcus radiodurans is 0.02-0.04 g / ml after the bio-adsorbent is added. The pH value of the Pb<2+>-containing solution to be treated is regulated to 2.0-4.0, then the bio-adsorbent is added, the final concentration of the adsorbent is 0.02-0.04 g / ml, stirring reaction is carried out for 3 minutes or more, then Pb<2+> in a water body is removed, and the strains are separated from the solution by filtration, centrifugation or natural precipitation. The water body has radioactivity or has the temperature higher than 50 DEG C.

The invention discloses an application of a radiation resistant bacteria Deinococcus wushiensis CGMCC No. 1.8884 in the biological treatment of heavy metal ions. The invention is characterized by: inoculating the bacterial strain Deinococcus wushiensis CGMCC No. 1.8884 to a liquid medium filled with 5ml TGY for cultivation at the temperature of 30 DEG C, carrying out a 200rpm shake cultivation for 36h, then cultivating according to an inoculation amount of 2%. The bacterial strain used in the invention has tolerance to Hg2+, Cu2+, Co2+, Pb2+ and Cr2+, wherein, the tolerance to Pb2+ and Cr2+ can reach to 150mg / L and 100mg / L respectively, and the tolerance to Cu2+ and Co2+ can reach to 80mg / L. The invention can be widely used in the field of environmental protection treatment of heavy metal waste liquor.

The invention provides a method for producing natural carotenoid by cultivating deinococcus radiodurans, which is obtained through collection of soybean whey, edulocration, adjusting of potential of hydrogen (pH), culture adding, sterilization, activation of strain, seed cultivation, fermenting and cultivation, wall-breaking of thallus, extraction of carotenoid and preparation of dried products. The method utilizes by-product of the soybean whey in a processing process of soya bean products such as bean curd to cultivate bacteria to ferment and produce the natural carotenoid, achieves waste recovery of the soybean whey, improves resource use efficiency, reduces pollution on environment caused by soya bean product processing enterprises, and is favorable for protecting environment. 229.8 mg carotenoid dried products can be obtained from per liter of the soybean whey fermentation broth and are 226.2% higher than carotenoid dried products obtained through a conventional cultivation fermenting extraction method. In the method, processing of acid-heating and wall-breaking is firstly conducted on somatic cells, then ethanol is used for extraction, and the extraction is complete and fast. The method is simple in production technology process, low in production cost. Products are free of toxic residue and high in safety.

The invention provides a method for immobilizing deinococcus radiodurans with functional magnetic carriers and an application of the method in treatment of uranium-bearing wastewater. An organic reagent is adopted for surface acyl chloride functionalization of magnetic Fe3O4 nanoparticles to obtain DR (deinococcus radiodurans) immobilizing carriers, the DR immobilizing carriers are magnetically immobilized with diethylenetriamine chemically-modified DR to obtain a functional magnetic adsorbent resistant to deinococcus radiodurans, and the functional magnetic adsorbent resistant to deinococcus radiodurans is applied to adsorption of the uranium-bearing wastewater. The novel functional magnetic adsorbent resistant to deinococcus radiodurans has the advantages of high radiation resistance, low cost, high chemical stability, high mechanical strength, effectiveness in uranium adsorption and the like. In addition, the method is simple and easy to realize magnetic control, and uranium content of low-concentration uranium-bearing wastewater with the concentration being 0.45-0.55mg / L can be reduced by more than 95%.

The invention finds that an HIN protein gene (DR1372) in deinococcus radiodurans R1 can improve anti-adversity ability of prokaryotic organisms and plants. The invention constructs a recombinant vector containing the gene, and respectively introduces the vector into prokaryotic and eukaryotic host cells. Experiment proves that after the HIN protein gene (DR1372) is expressed in prokaryotic host cells and plants, the salt tolerance of both the prokaryotic host cells and plants is improved.

The invention discovers that trkA genes (DR1666) in deinococcus radiodurans R1 have the function of enhancing stress tolerance of prokaryotes and plants. Recombinant vectors with such genes are built and respectively led into prokaryotic and eukaryotic host cells. The test proves that the trkA genes (DR1666) can enhance drought resistance after being expressed in the prokaryotic host cells and rapes.

The present invention relates to composition and methods of producing bioenergy. More specifically, the invention relates to the use of bacterium of the genus Deinococcus and / or related genera for the modification of biomass or biomass derivatives with a view to producing bioenergy products and metabolites.

The invention relates to processes of culture of a Deinococcus or a related bacterium using an oxidizer in conditions suitable for allowing the growth of Deinococcus and decreasing the growth of at least one other microorganism.

The invention discloses Deinococcus wushiensis RV12CGMCC No.6675 and application thereof in cobalt ion biological adsorption treatment. A strain is inoculated to medium containing 5ml TGY (tryptone glucose yeast) liquid and cultured at 30 DEG C. After shaking culture at 200rpm for 36h, the strain is cultured according to 2% inoculated culture. The cultured strain is tolerant to Hg2+, Cu2+, Co2+, Pb2+ and Cr2+, the tolerance to the Pb2+ and the Cr2+ is up to 150mg / L and 100mg / L respectively, and the tolerance to the Cu2+ and the Co2+ is up to 80mg / L. The Deinococcus wushiensis is widely applicable to the field of environmental treatment of waste liquid containing heavy metals, and is especially important to the application in biological adsorption treatment of cobalt ions.

The invention discloses a feed additive for improving laying yield of female silkworm moths, which is formed by mixing deinococcus radiopugnance (D. radiopugnance) after being smashed and zinc sulfate heptahydrate (ZnSO4.7H2O) according to a mass ratio of 1: 0.95 to 1.05. The invention also provides a preparation method of the feed additive. The D. radiopugnance is smashed through the following steps that: seed inocula of the D. radiopugnance with a reservation number of ATCC 19172 is inoculated to a fermentation culture medium to be cultured, and the acquired fermentation liquid is processedfor 40 to 60s under the condition with the frequency of 600 to 700MHz and the electric field strength of 8000 to 9000V / em after being centrifuged; and finally the smashed D. radiopugnance is acquiredafter being dried.

The invention discloses an amylosucrase mutant as well as a preparation method and application thereof, and belongs to the field of gene engineering and enzyme engineering. According to the amylosucrase mutant as well as the preparation method and application thereof, glycine at the 399th site of amylosucrase derived from Deinococcus Geothermals is mutated into serine, and the obtained amylosucrase mutant is applied to the production of turanose; the yield of the turanose obtained through the amylosucrase mutant under the conditions that the temperature is 35 DEG C, and the initial pH value is7.0 reaches up to 32% and is 2 times of the conversion rate of the turanose produced through wild enzymes; and therefore, the amylosucrase mutant can be applied to preparation of the turanose, so that the conversion rate of the turanose is further increased.

The invention discloses deinococcus radiodurans DNA transmethylase. The DNA transmethylase is derived from deinococcus radiodurans, and has typical DNA transmethylase conserved structural domains which are an Adomet binding domain containing a 'FxGxG' conserved sequence, a target sequence recognition domain (a TRD sequence) and a catalytic domain containing a 'TSPPY' conserved sequence successively from a N-terminal to a C-terminal, and belongs to Alpha-type N4-Cytosine DNA transmethylase. A substrate DNA conserved sequence recognized by the DNA transmethylase is 5'-CCGCGG-3', a position of methylated modification is a N4 site of a second cytosine C, and 4 mC type of a modified base is generated. An optimum temperature of a methylation reaction thereof is between 25 and 37 DEG C. The related DNA transmethylase is capable of specific-recognizing a 'CCGCGG' conserved sequence motif and methylated-modifying the N4 site of the second cytosine C thereof, and generating the 4mC modified base, and is the N4-Cytosine DNA transmethylase.

The present invention relates to a recombinant Deinococcus bacterium comprising a heterologous biosynthetic pathway converting lycopene to astaxanthin or a precursor thereof, and its use for producing astaxanthin or a precursor thereof. Shown is the production of carotene, zeaxanthin, canthaxanthin and astaxanthin using recombinant Deinococcus expressing genes encoding for lycopene cylase (EC 5.5.1.19), beta-carotene hydroxylase (EC1.14.13.129) and beta-carotene ketolase, namely carotenoid-4,4-beta-ionone ring oxygenase (EC 1.14.11.B16).

The invention discloses DNA (deoxyribonucleic acid) methyltransferase, and a soluble heterologous expression method and an isolation and purification method for the DNA methyltransferase. The DNA methyltransferase, the soluble heterologous expression method and the isolation and purification method have the advantages that the N4-Cytosine DNA methyltransferase M.DraR1 which has the DNA methyltransferase activity and is soluble in buffer solution is isolated and purified from deinococcus radiodurans for the first time; nucleotide sequences of the DNA methyltransferase, and the clone and expression method and the separation and purification method for the DNA methyltransferase are further provided; methodological reference can be provided to development and application of other DNA methyltransferase or restriction incision enzymes by the clone and expression method and the isolation and purification method, and the clone and expression method and the isolation and purification method have important guiding significance in developing novel molecular biological tool enzymes.

Genes have been isolated from Rhodococcus and Deinococcus which encode a specific lycopene β-cyclase capable of converting acyclic carotenoids with at least one ψ-end group to the corresponding asymmetric carotenoid containing a single β-ionone ring end group. The genes are new. Transformed host cells expressing the present genes and methods for the bio-conversion of acylic carotenoid substrates to corresponding asymmetric carotenoid are also provided.

The invention provides a deinococcus radiodurans-derived P450 monooxygenase mutant and application thereof in synthesis of (S)-2-chloro-1-phenylethanol chiral beta-halohydrin compounds. The P450 monooxygenase is subjected to several rounds of directed transformation to obtain P450 monooxygenase mutants N190D, N190F and N190F / V356L / A486E with improved asymmetric hydroxylation activity, wherein thecatalytic activity of the P450 monooxygenase mutant N190F / V356L / A486E is increased by 8.5 times that of wild P450 monooxygenase. The whole cell of the N190F / V356L / A486E mutant can be applied to catalysis of a benzylic asymmetric hydroxylation reaction of 1-chloro-2-phenylethane and derivatives thereof, the corresponding (S)-2-chloro-1-phenylethanol chiral beta-halohydrin compounds having the yieldof 88 percent and the optical purity of 98 percent ee can be obtained.

The invention relates to a method for synthesizing 5-hydroxymethyl furoic acid by utilizing deinococcus radiodurans R1. The method comprises the steps of streaking and inoculating deinococcus radiodurans R1 in a TGY solid culture medium, picking out single colonies after culture and inoculating the single colonies in a TGY liquid culture medium, culturing the single colonies to a logarithmic growth period, inoculating the single colonies into a fresh TGY liquid culture medium according to the inoculation amount of 1%, culturing for 48h, collecting thallus cells, adding the thallus cells into abuffer solution containing 5-hydroxymethyl furfural, executing reaction at the temperature of 20-60 DEG C for 3-48h to obtain the 5-hydroxymethyl furoic acid. According to the method, the deinococcusradiodurans R1 is utilized as a biocatalyst, has high tolerance to the 5-hydroxymethyl furfural, can catalyze a high-concentration substrate to be selectively oxidized and synthesized into a target product, the yield reaches 86% or above, and the method has the advantages of higher substrate concentration, more excellent reaction efficiency and good selectivity.

There are provided peptide tags derived from bacteriophytochrome (BphP) that is photoreceptor protein of Deinococcus radiodurans, an antibody capable of specifically recognizing the peptide tags, hybridoma cell lines capable of producing the antibody, and uses thereof. The novel peptide tag has advantages in that it has a short length and can remove a non-specific reaction of the conventional c-myc tag and FLAG tag. Therefore, in the case of using the novel peptide tag and antibody thereto, the fusion protein expressed in a recombinant cell can be very effectively detected or purified. In addition, an epitope tagging system including the novel peptide tag and antibody thereto can be applied in various fields such as a determination of an intracellular site, a confirmation of functionality, detection and purification of specific protein, and researches on interaction between proteins.

The invention finds that an HIN protein gene (DR1372) in deinococcus radiodurans R1 can improve anti-adversity ability of prokaryotic organisms and plants. The invention constructs a recombinant vector containing the gene, and respectively introduces the vector into prokaryotic and eukaryotic host cells. Experiment proves that after the HIN protein gene (DR1372) is expressed in prokaryotic host cells and plants, the salt tolerance of both the prokaryotic host cells and plants is improved.

The invention discloses a preparation method of nano-gold, the nano-gold and application. The preparation method of the nano-gold comprises the following steps: (1) after crushing deinococcus radiodurans thalli, taking out supernatant to prepare a nano-gold synthesis agent; (2) adding a solution containing Au<3+> into the nano-gold synthesis agent and reacting; (3) collecting sediment and washingto obtain the nano-gold. According to the preparation method disclosed by the invention, extract of deinococcus radiodurans is used for preparing the nano-gold and the nano-gold can be efficiently, greenly and conveniently synthesized; the synthesized nano-gold has good purity and a moderate size (about 51.72nm); the surface of the nano-gold has negative electricity and the nano-gold basically hasno cytotoxicity; the deinococcus radiodurans extract is used as a metal nano material and has the advantages which do not belong to common plant and bacterium extract.

The invention discloses an amylosucrase mutant as well as a preparation method and application thereof, and belongs to the field of gene engineering and enzyme engineering. According to the amylosucrase mutant as well as the preparation method and application thereof, glycine at the 399th site of amylosucrase derived from Deinococcus Geothermals is mutated into serine, and the obtained amylosucrase mutant is applied to the production of turanose; the yield of the turanose obtained through the amylosucrase mutant under the conditions that the temperature is 35 DEG C, and the initial pH value is7.0 reaches up to 32% and is 2 times of the conversion rate of the turanose produced through wild enzymes; and therefore, the amylosucrase mutant can be applied to preparation of the turanose, so that the conversion rate of the turanose is further increased.

The present invention relates to a recombinant Deinococcus bacterium exhibiting enhanced 2-C-methyl-D-erythritol 4-phosphate / 1-deoxy-D-xylulose 5-phosphate (MEP / DXP) pathway, and its use for producing terpene or terpenoid compounds.

The invention discloses DNA (deoxyribonucleic acid) methyltransferase, and a soluble heterologous expression method and an isolation and purification method for the DNA methyltransferase. The DNA methyltransferase, the soluble heterologous expression method and the isolation and purification method have the advantages that the N4-Cytosine DNA methyltransferase M.DraR1 which has the DNA methyltransferase activity and is soluble in buffer solution is isolated and purified from deinococcus radiodurans for the first time; nucleotide sequences of the DNA methyltransferase, and the clone and expression method and the separation and purification method for the DNA methyltransferase are further provided; methodological reference can be provided to development and application of other DNA methyltransferase or restriction incision enzymes by the clone and expression method and the isolation and purification method, and the clone and expression method and the isolation and purification method have important guiding significance in developing novel molecular biological tool enzymes.

The invention discovers that pprM gene in Deinococcus radiodurans R1 can enhance the drought resistance of prokaryotes and plants. Recombinant vectors comprising the gene are constructed and respectively introduced into procaryotic and enkaryotic host cells. Experiments prove that after the pprM gene is expressed in prokaryotic host cells and plants, the resistance to drought environment can be enhanced.