31 results about "Deinococcus" patented technology

The invention relates to recombinant escherichia coli THRS-6 and a method for synthesizing L-threonine by using the recombinant escherichia coli THRS-6. The invention belongs to the technical field of bioengineering, and relates to an aspartic acid kinase thrAmut and pyridine nucleotide transhydrogenase pntAB gene derived from escherichia coli, an aspartic acid dehydrogenase aspDH gene derived from pseudomonas aeruginosa, a hemoglobin vgb gene derived from vitreoscilla and a transcriptional regulation factor irrE gene derived from radiococcus. The recombinant escherichia coli is used as a substrate, the ldhA gene is knocked out to further modify the recombinant escherichia coli, the recombinant escherichia coli THRS-6 with glucose as the substrate is obtained, the THRS-6 is used for fermentation, and the yield and conversion rate of the L-threonine are obviously improved.

The present invention relates to multiple polypeptides with high safety, cell repair capacity, and anti-inflammatory, hydrating and moisturizing effects, and the use thereof in cosmetics, wherein the amino acid sequences of the polypeptides are as set forth in SEQ ID NO: 1-SEQ ID NO: 6. The present invention also relates to a polynucleotide encoding the polypeptides, wherein the sequence of the polynucleotide is as set forth in SEQ ID NO: 7-SEQ ID NO: 12, and the sequence of the polynucleotide can be used for the detection of Deinococcus weizhi. In addition, further provided is a topical drug or a cosmetic, which contains any one of the polypeptides or a combination thereof as an active ingredient.

The present invention provides a novel microbial strain resource, specifically relates to the identification of Deinococcus Weizhi as a new species, and provides specific Deinococcus weizhi VB142 and Deinococcus weizhie VB226. A fermentation broth, a fermentation product filtrate, a fermentation lysate, and extracts of the fermentation broth can be obtained by means of conventional fermentation, are applicable for the preparation of antioxidant products, and have wide application value and market prospects.

The present invention provides a novel microbial strain resource, specifically relates to the identification of Deinococcus Weizhi as a new species, and provides specific Deinococcus weizhi VB142 and Deinococcus weizhie VB226. A fermentation broth, a fermentation product filtrate, a fermentation lysate, and extracts of the fermentation broth can be obtained by means of conventional fermentation, are applicable for the preparation of antioxidant products, and have wide application value and market prospects.

The invention discloses a method for improving the yield of L-threonine by enhancing Escherichia coli electronic respiration transfer and energy transfer efficiency, and belongs to the technical field of genetic engineering. According to the invention, a cytochrome bo oxidase subunit CyoA mutant derived from escherichia coli, a cytochrome bo oxidase subunit CyoB mutant derived from pseudomonas aeruginosa, a cytochrome bd oxidase cydAB gene cluster derived from escherichia coli and a polyphosphate kinase PPK mutant derived from radiation-resistant coccus are introduced, amn genes are knocked out, and a cytochrome bo oxidase gene is obtained. The electron transfer efficiency in escherichia coli and the production efficiency of energy ATP (adenosine triphosphate) are enhanced, and the production efficiency of L-threonine and the carbon yield are further improved. Finally, the obtained engineering strain ECTHRS-5 is subjected to fed-batch fermentation in a 5L fermentation tank to accumulate 160.8 g / L of L-threonine, and the sugar-acid conversion rate is 0.62 g / g.

The application belongs to the field of microbial degradation of dyes, and relates to application of deinococcus in degradation of malachite green wastewater. The deinococcus is Deinococcus wulumuqiensis R12. When the initial concentration of malachite green MG is 200 mg / L, the cell concentration of R12 is 8.0 g / L, the reaction is carried out at 30 DEG C and pH 6.0 for 30 min, the degradation rate is 99.80%. When the initial concentration of MG is 1000 mg / L, the degradation rate can reach more than 95% after 1.5 h of reaction. Meanwhile, the R12 has wide adaptability to pH and temperature in degrading malachite green, and can efficiently degrade malachite green MG under the conditions of pH 5.0-10.0 and 10-45 DEG C.

The present invention provides a salt-resistant and heavy metal-resistant Deinococcus and its application in rice and wheat, belonging to the technical field of microorganism-plant interaction and its application. Deinococcus sp. )NH1, deposited in China Center for Type Culture Collection on July 22, 2024, with the deposit address: Wuhan University, Wuhan, China; the deposit number is CCTCC NO: M 20241625; a microbial agent, comprising the Deinococcus ( Deinococcus sp. )NH1; the Deinococcus ( Deinococcus sp. ) Application of NH1 or the above-mentioned microbial agents in promoting plant growth.

The present invention provides a nicotinamide phosphoribosyltransferase (Nampt) mutant and its application in the preparation of β-nicotinamide mononucleotide (β-Nicotinamide Mononucleotide, referred to as NMN), belonging to the field of enzyme engineering technology. The present invention screens nicotinamide phosphoribosyltransferases from different sources and finds that the mutant from Diplococcus cellulosus ( Deinococcus cellulosilyticus ) has the highest Nampt activity. After mutation, it was found that the Nampt[Dc] E227N D334M double mutant had the highest activity, which was 1.9 times that of the wild type. The nicotinamide phosphoribosyltransferase and its mutants were used as biocatalysts for the enzymatic synthesis of NMN. The catalyst conversion efficiency can reach up to 71%, and the NMN yield can reach up to 29.24g / L.

The invention relates to a plurality of polypeptides with high safety, cell repair capability, anti-inflammatory and moisturizing effects and application thereof in cosmetics, amino acid sequences of the polypeptides are shown as SEQ ID NO: 1-SEQ ID NO: 6, polynucleotides for encoding the polypeptides are also provided, the polynucleotide sequences are shown as SEQ ID NO: 7-SEQ ID NO: 12, and the polynucleotides are used for encoding the polypeptides. The polynucleotide sequence can be used for detecting deinococcus micrococci; the invention also provides an external medicine or a cosmetic, which comprises any one or a combination of the polypeptide as an active ingredient.

The invention provides a microbial marker for schizophrenia with social anxiety disorder and application of the microbial marker. The microbial marker is a deinococcus microorganism in saliva. The relevance between the content of the deinococcus microorganisms in saliva and social anxiety disorder is found for the first time. Moreover, by taking deinococcus microorganisms in saliva as potential microbial markers, objective biological indexes can be provided for early diagnosis and monitoring of the schizophrenia accompanied with social anxiety disorder, and errors of subjective evaluation are reduced; moreover, compared with the intestinal specific biomarker, the oral saliva sample has the obvious advantages of easiness in collection and the like for psychotic patients, and the working difficulty of medical personnel can be greatly reduced; besides, the specific microbial marker can be combined with a traditional psychological scale and other biomarkers to form a more comprehensive diagnosis mode, and revolutionary changes of prediction, diagnosis and treatment of schizophrenia with social anxiety disorder are expected to be brought.

The invention discloses a method for efficient traceless editing of a myxobacteria genome and application. According to the invention, a TnpB cutting system ISDra2 of deinococcus radiodurans is combined with a homologous recombination system MxRedET of myxobacteria, so that a one-step traceless editing platform (MxDIRECT) is formed. The ISDra2 is responsible for accurately positioning and cutting target DNA, and the MxRedET immediately realizes efficient fragment integration or replacement at a cut, so that operations such as gene knockout, insertion and replacement are realized. According to the method, the editing efficiency of myxobacteria is improved to 100% from original 1%, the period is shortened to 5-7 days from 3-6 months, and one-step double-site traceless editing can be achieved through double-site design. The longest DNA of about 80 kb can be knocked out through a single incision, and the longest DNA can reach 200 kb through double incisions; and mononucleotide replacement can be carried out at any position, so that accurate base editing is realized. The technology provides an efficient and accurate tool for modifying functional genes and metabolic pathways of the myxobacteria chassis cells, and obviously promotes the research of synthetic biology.

The invention discloses a high-temperature-resistant heterococcus and application thereof, and belongs to the technical field of algae. A high-temperature-resistant heterococcus sp. LBV11 strain is screened from Tengchong hot spring in Yunnan province and is preserved in the China Center for Type Culture Collection (CCTCC), and the preservation number is CCTCC M 20241316. Through biomass monitoring and culture condition optimization, rapid culture of the heterococcus is successfully realized. The Xenococcus sp. LBV11 screened by the invention can stably grow and keep good activity under the conditions that the temperature is 40 to 50 DEG C, the illumination is 12 to 16 hours / the dark environment is 8 to 12 hours, the illumination condition is 3000 to 5000 Lux, and the humidity is 60 to 65 percent. The algal strain is rich in nutritional ingredients and high in protein content, and phycocyanin in the algal strain has remarkable thermal stability. And when being cultured in the culture wastewater, the strain has relatively strong absorption capacity on main pollutants such as nitrogen and phosphorus in the culture wastewater, and shows an excellent effect in the aspect of water purification. Therefore, the heterococcus has wide application potential and commercial value in the fields of feed additives and water treatment, provides a new idea and an efficient solution for ecological treatment of aquaculture wastewater, and has a good market application prospect.

The invention provides a cytochrome P450 hydroxylase mutant and application thereof. The amino acid sequence of the cytochrome P450 hydroxylase mutant is shown as SEQ ID NO. 1. According to the present invention, protein modification is performed on the enzyme activity of P450 derived from a Deinococcus apache wild type strain and the S-stereoselectivity of an asymmetric hydroxylation reaction by using a directed evolution means, such that the activity and the selectivity of the enzyme are improved, and a series of the P450 enzyme mutants capable of being used for industrial production of the S-stereoselective halogenated allyl alcohol are developed; moreover, the whole operation process for producing the S-stereoselective halogenated allyl alcohol is simple, the process is mature and environment-friendly, and the method has a wide application prospect.

The invention belongs to the field of microbial fermentation and metabolites thereof, and particularly relates to a fermentation method for simultaneously producing superoxide dismutase and nano-silver particles and a product composition. According to the invention, the stress tolerance characteristic of deinococcus radiodurans is utilized, and the concentration of Ag < + > is controlled, so that the enzyme activity of oxidation-reduction enzymes including SOD (superoxide dismutase) of the deinococcus radiodurans is improved, and meanwhile, the Ag < + > is reduced into nano-silver particles (DR-Ag NPs). After deinococcus radiodurans is stressed by Ag < + >, the product is rich in oxidoreductase including SOD and nano-silver particles (DR-Ag NPs), the product can be prepared into a functional cosmetic product after being compounded with hyaluronic acid and the like, the functional cosmetic product has super-strong anti-aging, anti-wrinkle, whitening, antibacterial and anti-inflammatory effects and the like, and meanwhile, the use of preservatives is avoided.

The invention provides a functional pet food based on deinococcus radiodurans and a preparation method thereof, and the functional pet food based on deinococcus radiodurans comprises a functional pet food base material and an additive, the mass percentage of the functional pet food base material is 92-99 wt%; the mass percentage of the additive is 1-8wt%; the additive contains carotenoid with the mass percentage of 0.01-0.5 wt%, and the carotenoid is extracted from deinococcus radiodurans. The functional pet food containing the carotenoid with the mass percentage of 0.01-0.5 wt%, provided by the invention, can be beneficial to preventing oxidative damage of brains of middle-aged and aged cats and improving intestinal flora of the middle-aged and aged cats, so that the oxidation resistance of the middle-aged and aged cats is improved, the activity of the middle-aged and aged cats is enhanced, the health of the middle-aged and aged cats is guaranteed, and the aging of the middle-aged and aged

This invention discloses a Gobi abnormal coccus ( Deinococcus gobiensis Genes encoding ferritin in I-0 DgfE2 The nucleotide sequence of this gene is shown in SEQ ID NO. 1. The protein DgfE2 encoded by this gene has salt-tolerant function and can be used to cultivate salt-tolerant microorganisms, thus providing a reference for cultivating plants with salt-tolerant traits. The amino acid sequence of this protein is shown in SEQ ID NO. 2. After being subjected to 0.6 mol / L NaCl stress for 5 h, it contains... DgfE2 Salt-tolerant recombinant strain pET28a- DgfE2 The growth of the / BL21 strain was better than that of the control pET28a / BL21 strain containing only an empty plasmid, indicating that this recombinant strain has the ability to tolerate salt stress. DgfE2 DgfE2 The gene has the function of improving the salt tolerance of E. coli.

The present invention relates to multiple polypeptides with high safety, cell repair capacity, and anti-inflammatory, hydrating and moisturizing effects, and the use thereof in cosmetics, wherein the amino acid sequences of the polypeptides are as set forth in SEQ ID NO: 1-SEQ ID NO: 6. The present invention also relates to a polynucleotide encoding the polypeptides, wherein the sequence of the polynucleotide is as set forth in SEQ ID NO: 7-SEQ ID NO: 12, and the sequence of the polynucleotide can be used for the detection of Deinococcus weizhi. In addition, further provided is a topical drug or a cosmetic, which contains any one of the polypeptides or a combination thereof as an active ingredient.

In one aspect, the invention relates to a TnpB variant polypeptide comprising a Deinococcus radiodurans (ISDra2) TnpB polypeptide sequence and, separated by a peptide linker, a nuclear localization module comprising at least two nuclear localization sequences (NLS) separated by a first peptide linker. A second aspect of the invention relates to a DNA editing polypeptide comprising the TnpB variant polypeptide according to the invention, wherein the TnpB variant polypeptide comprises a nuclease deactivating mutation, and a nucleoside deaminase. The invention further relates to a nucleic acid sequence encoding a polypeptide according to the invention, and to a virus comprising the nucleic sequence.

The invention provides a deinococcus radiodans and saussurea involucrata co-fermentation product as well as a preparation method and application thereof, and belongs to the technical field of cosmetic raw material development. According to the invention, deinococcus radiodurans and saussurea involucrata are subjected to co-fermentation, such that a deinococcus radiodurans and saussurea involucrata co-fermentation product is obtained. An anti-oxidation experiment shows that the expression of catalase genes and glutathione peroxidase genes after UVB irradiation is remarkably reduced, and the expression of the catalase genes and the glutathione peroxidase genes is remarkably increased after the co-fermentation product of the deinococcus radiodurans and the saussurea involucrata provided by the invention is added, so that the co-fermentation product has an obvious anti-oxidation effect; a cDNA repair experiment shows that the content of pyrimidine dimer is increased after UVB irradiation, the content of pyrimidine dimer is remarkably reduced after the deinococcus radiodurans and saussurea involucrata co-fermentation product is added, and it is proved again that the product has the excellent anti-oxidation effect, and the application prospect is wide. The deinococcus radiodurans and saussurea involucrata co-fermentation product can be used as an anti-ultraviolet radiation cosmetic raw material, and has a very good application prospect.

The present application belongs to the field of microbial fermentation and its metabolites, and particularly relates to a fermentation method and product composition for simultaneously producing superoxide dismutase and nano silver particles. The present application utilizes the stress tolerance characteristics of Deinococcus radiodurans, controls the concentration of Ag+, improves the enzyme activity of the oxidoreductase of Deinococcus radiodurans including SOD, and at the same time, Ag+ is reduced into nano silver particles (DR-Ag NPs). After the stress of Ag+ on Deinococcus radiodurans, the product is rich in oxidoreductase including SOD and nano silver particles (DR-Ag NPs), and after compounding with hyaluronic acid and the like, a functional cosmetic product can be prepared, which has super strong anti-aging, anti-wrinkle, whitening, antibacterial and anti-inflammatory effects, and at the same time, the use of preservatives is avoided.

The invention provides a preparation method of a laccase-like SiO2 / CeO2 composite nanomaterial, which adopts a biosynthesis method and comprises the following steps: S1, synthesis of a precursor SiO2: uniformly mixing hexadecyl trimethyl ammonium bromide, a sodium hydroxide aqueous solution and ethanol in deionized water, dropwise adding tetraethyl orthosilicate, and centrifuging, washing and calcining after the reaction is finished to obtain SiO2; s2, synthesis of the SiO2 (at) CeO2 composite nanomaterial: mixing the SiO2 aqueous solution prepared in the step S1 and the (CH3CO2) 3Ce aqueous solution, adding a deinococcus radiodurans R12 cell-free supernatant in a stable phase for incubation, centrifuging, washing, collecting precipitate after incubation is finished, freeze-drying overnight, and calcining to obtain the SiO2 (at) CeO2 composite nanomaterial. The laccase-like SiO2 / CeO2 composite nano material is successfully synthesized by using a sterile supernate system in a growth stable period of deinococcus radiodurans, and the laccase-like blank of a non-copper-based nano material is filled. And the preparation method is simple, low in cost and easy to implement, and lays a solid foundation for industrial production of the laccase-like enzyme.

The invention discloses bacillus toonensis, which is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation date is February 2, 2024, and the preservation number is CGMCC No.29852. The bacillus toonensis belongs to the eubacterium field, the posterior mycophylum, the bacilli, the bacillus family and the bacillus genus. The bacterial suspension of the bacillus orientalis can be used for degrading cellulose, starch and straw.

A recombinant Escherichia coli THRS-6 and a method for synthesizing L-threonine thereof. The present invention belongs to the technical field of bioengineering, and overexpresses aspartokinase thrA <supgt; mut < / supgt; and pyridine nucleotide transhydrogenase pntAB genes, aspartate dehydrogenase aspDH gene derived from Pseudomonas aeruginosa, hemoglobin vgb gene derived from Vitreoscilla sp., and transcriptional regulator irrE gene derived from Deinococcus radiodurans, and further modifies the recombinant Escherichia coli by knocking out ldhA genes, obtaining a recombinant Escherichia coli THRS-6 using glucose as a substrate. Fermentation is carried out using the THRS-6, and the yield and conversion rate of L-threonine are significantly improved.

The invention discloses a complex microbial inoculant for repairing saline-alkali soil and application of the complex microbial inoculant, and belongs to the technical field of microorganisms. The preparation method comprises the following steps: respectively carrying out fermentation culture on sphingobacterium bambusae and deinococcus cellulosilyticus, carrying out centrifugal resuspension, and mixing the obtained bacterial solutions, so as to prepare the composite bacterial agent for repairing the soil of the saline-alkali land. The preparation method comprises the following steps: carrying out fermentation culture on the sphingobacterium bambusae and the deinococcus cellulosilyticus respectively, and carrying out centrifugal resuspension on the obtained bacterial solutions, so as to obtain the composite bacterial agent for repairing the soil of the saline-alkali land. According to the invention, the complex microbial inoculant is applied to saline-alkali soil by using crushed corn straw as a carrier; experimental results show that the complex microbial inoculant can efficiently reduce the pH value and salt content of the saline-alkali soil and improve the organic matter content of the saline-alkali soil. A novel microbial material and an efficient remediation method are provided for improvement and remediation of the saline-alkali soil, the steps are simple, the effect is lasting and stable, and the method has prominent practical application value and wide application prospects.

The invention discloses a microbial marker, a diagnostic model and a kit for early diagnosis of sensorineural deafness and application. The microbial marker comprises at least one of abnormal coccus, cilia, Ackermann muciniphile and actinomycetes. Experiments find that compared with healthy control, the relative abundance of abnormal coccus, cilia, Ackerman muciniphile and actinomycetes on the skin surface of the external auditory canal of a patient suffering from SSHL (sensorineural deafness) is obviously increased, and by detecting the relative abundance of the microbial marker, high-accuracy early diagnosis and evaluation of SSHL are achieved, so that the SSHL can be used for early diagnosis and evaluation of the external auditory canal of the patient suffering from the sensorineural deafness. Therefore, early intervention and treatment of diseases are facilitated. Besides, the method is simple and convenient in sampling and simple in operation method, is widely applicable to people with poor cognition and compatibility, realizes noninvasive and age-limit-free SSHL early screening, and has wide market application prospects and social benefits.

The invention provides a new microbial strain resource, particularly relates to identification of Deinococcus weishi as a new strain, and provides specific Deinococcus weishi VB142 and Deinococcus weishi VB226 which are subjected to conventional fermentation to obtain fermentation liquor, fermentation product filtrate, fermentation lysate and extract of the fermentation liquor, and the fermentation liquor, the fermentation product filtrate, the fermentation lysate and the extract of the fermentation liquor can be used for preparing antioxidant products. Wide application values and market prospects are realized.

The invention relates to application of thioredoxin DrTrx2 coming from deinococcus radiodurans and having an anti-radiation function in preparation of drugs, the drugs are used for improving the anti-radiation capacity of organisms, and the amino acid sequence of the thioredoxin DrTrx2 is shown as SEQ ID NO.3. The thioredoxin DrTrx2 has the anti-radiation function, is most sensitive when responding to heavy ion radiation, and has the anti-radiation function. And the single protein has an anti-radiation function and can be used as a radiation-resistant functional component.

The invention belongs to the technical field of radioactive nuclear drugs, and particularly relates to a method for preparing a targeted nuclear drug by reducing nuclide lutetium-177 through a biological preparation, and the method comprises the following steps: step 1, activating and culturing a deinococcus radiodurans strain to obtain a deinococcus radiodurans thallus; and 2, adding the deinococcus radiodurans thalli into a nuclide lutetium chloride 177 LuCl3 solution, and incubating, so as to obtain the deinococcus radiodurans targeted nuclear drug loaded with lutetium 177. The deinococcus radiodurans obtained by the method provided by the invention has strong adsorption effect and binding capacity on lutetium-177, and can effectively reduce the preparation cost of the targeted nuclear drug; the prepared targeting nuclear drug is high in biological safety and enhanced in targeting property.

A photoacoustic contrast agent based on a reversible optical switch fusion protein, a preparation method therefor, and use thereof. The photoacoustic contrast agent comprises a phytochrome protein and a collagen-binding domain fused to the C-terminus of the phytochrome protein. The phytochrome protein is derived from Deinococcus radiodurans. The photoacoustic contrast agent combines the phytochrome protein derived from Deinococcus radiodurans and the collagen-binding domain, ingeniously integrating the optical switch function of DrBphP and the high affinity of the CBD for collagen. The DrBphP-CBD fusion protein retains the high affinity for type I collagen and type III collagen and its optical switch property. The photoacoustic contrast agent can be specifically attached to exposed collagen by utilizing the non-typical vascular structure of tumors and the excessive deposition of collagen in fibrotic diseases, thereby facilitating continuous delivery and retention in the affected tissue microenvironment and providing enhanced optical switch photoacoustic differential imaging for pathological section staining and in vivo imaging detection of diseases.