9 results about "Deinococcus" patented technology

The present invention provides a novel microbial strain resource, specifically relates to the identification of Deinococcus Weizhi as a new species, and provides specific Deinococcus weizhi VB142 and Deinococcus weizhie VB226. A fermentation broth, a fermentation product filtrate, a fermentation lysate, and extracts of the fermentation broth can be obtained by means of conventional fermentation, are applicable for the preparation of antioxidant products, and have wide application value and market prospects.

The application belongs to the field of microbial degradation of dyes, and relates to application of deinococcus in degradation of malachite green wastewater. The deinococcus is Deinococcus wulumuqiensis R12. When the initial concentration of malachite green MG is 200 mg / L, the cell concentration of R12 is 8.0 g / L, the reaction is carried out at 30 DEG C and pH 6.0 for 30 min, the degradation rate is 99.80%. When the initial concentration of MG is 1000 mg / L, the degradation rate can reach more than 95% after 1.5 h of reaction. Meanwhile, the R12 has wide adaptability to pH and temperature in degrading malachite green, and can efficiently degrade malachite green MG under the conditions of pH 5.0-10.0 and 10-45 DEG C.

The invention discloses a method for efficient traceless editing of a myxobacteria genome and application. According to the invention, a TnpB cutting system ISDra2 of deinococcus radiodurans is combined with a homologous recombination system MxRedET of myxobacteria, so that a one-step traceless editing platform (MxDIRECT) is formed. The ISDra2 is responsible for accurately positioning and cutting target DNA, and the MxRedET immediately realizes efficient fragment integration or replacement at a cut, so that operations such as gene knockout, insertion and replacement are realized. According to the method, the editing efficiency of myxobacteria is improved to 100% from original 1%, the period is shortened to 5-7 days from 3-6 months, and one-step double-site traceless editing can be achieved through double-site design. The longest DNA of about 80 kb can be knocked out through a single incision, and the longest DNA can reach 200 kb through double incisions; and mononucleotide replacement can be carried out at any position, so that accurate base editing is realized. The technology provides an efficient and accurate tool for modifying functional genes and metabolic pathways of the myxobacteria chassis cells, and obviously promotes the research of synthetic biology.

This invention discloses a Gobi abnormal coccus ( Deinococcus gobiensis Genes encoding ferritin in I-0 DgfE2 The nucleotide sequence of this gene is shown in SEQ ID NO. 1. The protein DgfE2 encoded by this gene has salt-tolerant function and can be used to cultivate salt-tolerant microorganisms, thus providing a reference for cultivating plants with salt-tolerant traits. The amino acid sequence of this protein is shown in SEQ ID NO. 2. After being subjected to 0.6 mol / L NaCl stress for 5 h, it contains... DgfE2 Salt-tolerant recombinant strain pET28a- DgfE2 The growth of the / BL21 strain was better than that of the control pET28a / BL21 strain containing only an empty plasmid, indicating that this recombinant strain has the ability to tolerate salt stress. DgfE2 DgfE2 The gene has the function of improving the salt tolerance of E. coli.

The present application belongs to the field of microbial fermentation and its metabolites, and particularly relates to a fermentation method and product composition for simultaneously producing superoxide dismutase and nano silver particles. The present application utilizes the stress tolerance characteristics of Deinococcus radiodurans, controls the concentration of Ag+, improves the enzyme activity of the oxidoreductase of Deinococcus radiodurans including SOD, and at the same time, Ag+ is reduced into nano silver particles (DR-Ag NPs). After the stress of Ag+ on Deinococcus radiodurans, the product is rich in oxidoreductase including SOD and nano silver particles (DR-Ag NPs), and after compounding with hyaluronic acid and the like, a functional cosmetic product can be prepared, which has super strong anti-aging, anti-wrinkle, whitening, antibacterial and anti-inflammatory effects, and at the same time, the use of preservatives is avoided.

The invention provides a preparation method of a laccase-like SiO2 / CeO2 composite nanomaterial, which adopts a biosynthesis method and comprises the following steps: S1, synthesis of a precursor SiO2: uniformly mixing hexadecyl trimethyl ammonium bromide, a sodium hydroxide aqueous solution and ethanol in deionized water, dropwise adding tetraethyl orthosilicate, and centrifuging, washing and calcining after the reaction is finished to obtain SiO2; s2, synthesis of the SiO2 (at) CeO2 composite nanomaterial: mixing the SiO2 aqueous solution prepared in the step S1 and the (CH3CO2) 3Ce aqueous solution, adding a deinococcus radiodurans R12 cell-free supernatant in a stable phase for incubation, centrifuging, washing, collecting precipitate after incubation is finished, freeze-drying overnight, and calcining to obtain the SiO2 (at) CeO2 composite nanomaterial. The laccase-like SiO2 / CeO2 composite nano material is successfully synthesized by using a sterile supernate system in a growth stable period of deinococcus radiodurans, and the laccase-like blank of a non-copper-based nano material is filled. And the preparation method is simple, low in cost and easy to implement, and lays a solid foundation for industrial production of the laccase-like enzyme.

The invention discloses a microbial marker, a diagnostic model and a kit for early diagnosis of sensorineural deafness and application. The microbial marker comprises at least one of abnormal coccus, cilia, Ackermann muciniphile and actinomycetes. Experiments find that compared with healthy control, the relative abundance of abnormal coccus, cilia, Ackerman muciniphile and actinomycetes on the skin surface of the external auditory canal of a patient suffering from SSHL (sensorineural deafness) is obviously increased, and by detecting the relative abundance of the microbial marker, high-accuracy early diagnosis and evaluation of SSHL are achieved, so that the SSHL can be used for early diagnosis and evaluation of the external auditory canal of the patient suffering from the sensorineural deafness. Therefore, early intervention and treatment of diseases are facilitated. Besides, the method is simple and convenient in sampling and simple in operation method, is widely applicable to people with poor cognition and compatibility, realizes noninvasive and age-limit-free SSHL early screening, and has wide market application prospects and social benefits.

The invention discloses a preparation method and application of a deinococcus radiodurans metagen. The preparation method comprises the following steps: (1) preparing liquid seeds; (2) solid state fermentation: 5-8% (v / w) of the liquid seed liquid obtained in the step (1) is inoculated to a solid fermentation culture medium, the solid fermentation culture medium is prepared from, by mass, 50-60% of soybean meal, 30-40% of sunflower seeds, 4-12% of flaxseed meal and 2-8% of palm pomace, and constant-temperature fermentation is performed at 28-32 DEG C for 48-60 h; and (3) post-treatment: after the fermentation is finished, drying the fermented material at a low temperature of 55-65 DEG C until the moisture is less than or equal to 5% to obtain the deinococcus radiodurans metagen powder. The microencapsulation embedding method comprises the following steps: (1) preparing a wall material solution, (2) dispersing a core material, (3) forming microcapsules, and (4) carrying out post-treatment.The technical problems that active substances in deinococcus radiodurans metabiotics are easy to degrade and low in bioavailability in the pet food processing and digestion process and cannot stably exert the functions of oxidation resistance and flora regulation are solved.