Application of radiation resistant bacteria in biological treatment of heavy metal ions

A heavy metal ion, radiation-resistant technology, used in biological water/sewage treatment, microorganism-based methods, bacteria, etc., can solve problems such as high operating costs, secondary pollution, and low removal rates

Inactive Publication Date: 2011-11-02
THE INST OF MICROBIOLOGY XINJIANG ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, the main methods for treating cobalt-containing waste liquid are sulfidation precipitation method [Chen Weiping, Yang Qingshan, He Bining, etc. Research on the recovery of cobalt in NdFeB waste. Rare metals and hard alloys, 2006, 34: 55-57], ammonium oxalate precipitation method [Tan Haixiang, Hu Qiyang, Li Xinhai, etc. New technology for cobalt recovery from spent lithium cobalt oxide pole pieces. Power Technology, 2007, 31: 288-230], hydrochloric acid reverse phase extraction method [Shuai Guoquan. Cobalt recovery of Jinchuan Company. Nonferrous smelting, 1995 (3): 15-18] and other methods, these treatment methods have achieved good results to a certain extent, but secondary pollution generally exists, especially when the concentration of heavy metals in water is low (<100mg / L), not only Lower removal rate and higher operating costs
At present, there is no report on the adsorption of cobalt ions

Method used

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  • Application of radiation resistant bacteria in biological treatment of heavy metal ions
  • Application of radiation resistant bacteria in biological treatment of heavy metal ions
  • Application of radiation resistant bacteria in biological treatment of heavy metal ions

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1: Liquid culture of strain Deinococcus wushiensis CGMCC No. 1.8884

[0020] The activated strain Deinococcus wushiensis of the present invention was inoculated into a seed test tube containing 5ml of TGY liquid medium, cultivated at 30°C and shaken at 200rpm for 36 hours, and then inoculated into a TGY liquid fermentation flask at 2% of the inoculum (filling amount It is a 500ml Erlenmeyer flask with 80ml TGY liquid medium), cultured at 30°C, shaking at 220rpm for 60h.

[0021] After the above-mentioned culture, the maximum concentration of bacteria can reach 4.51 OD / ml (600nm). The culture was centrifuged at 8000rpm for 3min, the supernatant was discarded, and the bacteria were collected. The wet weight of the bacteria could reach 5.2g / L.

Embodiment 2

[0022] Example 2: Strain Deinococcus wushiensis CGMCC No. 1.8884 heavy metal resistance

[0023] The activated strain of the present invention, Deinococcus wushiensis, was inoculated into a seed test tube containing 5ml of TGY liquid medium, cultured at 30°C and shaken at 200rpm for 36 hours, and then inoculated with 2% of the inoculum amount in different concentrations of Hg. 2+ , Cu 2+ , Co 2+ , Pb 2+ , Cr 2+ In a TGY liquid fermentation flask (a 500ml triangular bottle with 80ml TGY liquid medium), culture at 30°C, shaking at 220 rpm for 72 hours, and observe the growth of the bacteria. See attached for results figure 1 , By the attachment figure 1 It can be seen that the patent strain Deinococcus wushiensis is resistant to a variety of metals, among which Pb 2+ , Cr 2+ The maximum tolerance concentration (100mg / L) Cu 2+ , Co 2+ Second (80mg / L).

Embodiment 3

[0024] Example 3: The strain Deinococcus wushiensis CGMCC No. 1.8884 is in different Co 2+ Growth characteristics at different concentrations

[0025] The activated strain Deinococcus wushiensis of the present invention was inoculated into a seed test tube containing 5ml of TGY liquid medium, cultivated at 30°C and shaken at 200rpm for 36h, and then inoculated at 2% of the inoculum to contain 0, 20, 50, and 80 mg. / L Co 2+ In the TGY liquid fermentation bottle (the filling amount is 500ml triangular bottle with 80ml TGY liquid medium), cultivate at 30℃, shake culture at 220rpm for 120h, and regularly sample every 12h to determine the OD value of the bacteria, and observe the growth of the bacteria. The results show that the low concentration of Co 2+ ( figure 2 .

[0026] Table 1 Different concentrations of Co 2+ Effect on strain growth

[0027]

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Abstract

The invention discloses an application of a radiation resistant bacteria Deinococcus wushiensis CGMCC No. 1.8884 in the biological treatment of heavy metal ions. The invention is characterized by: inoculating the bacterial strain Deinococcus wushiensis CGMCC No. 1.8884 to a liquid medium filled with 5ml TGY for cultivation at the temperature of 30 DEG C, carrying out a 200rpm shake cultivation for 36h, then cultivating according to an inoculation amount of 2%. The bacterial strain used in the invention has tolerance to Hg2+, Cu2+, Co2+, Pb2+ and Cr2+, wherein, the tolerance to Pb2+ and Cr2+ can reach to 150mg/L and 100mg/L respectively, and the tolerance to Cu2+ and Co2+ can reach to 80mg/L. The invention can be widely used in the field of environmental protection treatment of heavy metal waste liquor.

Description

Invention field [0001] The present invention relates to the technical field of heavy metal biosorption. Specifically, the present invention relates to the technical field of application of radiation-resistant bacteria in heavy metal ion adsorption. Background technique [0002] Heavy metals refer to specific gravity greater than 4 or 5g / cm 3 There are about 45 kinds of metals, such as copper, zinc, lead, cobalt, manganese, cadmium, mercury and so on. Due to the increasing number of human mining, smelting, processing and commercial manufacturing activities of heavy metals, a variety of heavy metals such as lead, mercury, cadmium, copper, cobalt, etc. enter the atmosphere, water, and soil. With heavy metal ions in the environment or ecosystem Preservation, accumulation and migration cause serious environmental pollution and ecological harm. [0003] Traditional physical and chemical methods for treating heavy metal-containing wastewater include chemical precipitation, ion exchange, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F1/28C02F1/62C02F3/34C12R1/01C02F101/20
Inventor 张志东茆军唐琦勇王玮
Owner THE INST OF MICROBIOLOGY XINJIANG ACADEMY OF AGRI SCI
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