A liquid-actuated pump (100, 200, 300, 400) for a biological
treatment system, the pump (100, 200, 300, 400) comprising: a
pump chamber (130, 230, 330); a flexible diaphragm (132) configured to form a barrier between a control liquid portion (136) of the
pump chamber (130, 230, 330) and a process fluid portion (134) of the
pump chamber (130, 230, 330); a process fluid flow path (110, 210, 310) including a process fluid portion (134) of the pump chamber (130, 230, 330); and a control liquid flow path (120, 220) comprising: an inlet (122) to the pump (100, 200, 300, 400); an outlet (124) from the pump (100, 200, 300, 400); and a control liquid portion (136) of the pump chamber (130, 230, 330); wherein the control fluid flow path (120, 220) is configured to contain at least a portion of the control fluid of the trapped volume, and wherein displacement of the control fluid of the trapped volume causes deformation of the flexible diaphragm (132), the deformation of the flexible diaphragm (132) causing movement of the process fluid in the process fluid flow path (110, 210, 310).