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Radiation-resistant Aspergillus sp. and application thereof in cesium 137 adsorption biological treatment

A biological treatment and radiation-resistant technology, which is applied in the field of microbial strains for bioabsorption of radionuclides, radioresistant Aspergillus and its application in radionuclide adsorption, can solve the problem of limited ability to repair radioactively polluted environments

Inactive Publication Date: 2014-10-08
THE INST OF MICROBIOLOGY XINJIANG ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Since most bacteria are sensitive to radioactivity, their ability to remediate radioactively contaminated environments is limited
There are no reports about Aspergillus radiodurans and its application in radionuclide adsorption

Method used

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  • Radiation-resistant Aspergillus sp. and application thereof in cesium 137 adsorption biological treatment
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  • Radiation-resistant Aspergillus sp. and application thereof in cesium 137 adsorption biological treatment

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Experimental program
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Effect test

Embodiment 1

[0041] Embodiment one: Aspergillus ( Aspergillus sp. ) Isolation and cultivation of F77CGMCC No. 8382

[0042] 1. Separation: the radioresistant aspergillus used in the present invention ( Aspergillus sp. ) F77 was isolated from radioactively contaminated soil in a certain place in Bayingoleng Mongolian Autonomous Prefecture, Xinjiang, by the Institute of Microbiology Application of Xinjiang Academy of Agricultural Sciences. The microorganisms in the soil layer were isolated by traditional plate culture method, and the strain was purified by plate streaking method. The cultivation temperature, pH value, and culture medium were used as enrichment conditions, and a batch of well-growing microbial strains were screened out, and a strain numbered F77 was selected from them.

[0043] Separation steps: According to the gradient dilution method, weigh 10g of soil samples in 90mL of sterile saline, activate in a constant temperature shaking box at 30°C for 30min, and then carry o...

Embodiment 2

[0051] Embodiment two: aspergillus ( Aspergillus sp. ) Molecular level identification of F77CGMCC No. 8382

[0052] 1. DNA extraction: the fungal extraction method is as follows:

[0053] (1) 200 mg of bacterial cells were ground with liquid nitrogen, and 3 ml of 3% CTAB extraction buffer was added; placed in a water bath at 65°C for 45 minutes, and centrifuged at 4,000 r / min for 20 minutes at 4°C.

[0054] (2) Transfer the supernatant to a centrifuge tube, add 4 μl of 10 mg / ml protease, incubate at 37°C for 1 h.

[0055] (3) Add 800 μl Tris saturated phenol, shake well, and centrifuge at 13000 r / min for 10 min; take the supernatant.

[0056] (4) Add an equal volume of chloroform / isoamyl alcohol, shake well, and centrifuge at 13000 r / min for 10 min; take the supernatant.

[0057] (5) Add 10 mg / ml RNase, and treat overnight in a water bath at 37°C.

[0058] (6) Add 800 μl of chloroform / isoamyl alcohol, shake well, and centrifuge at 13000 r / min for 10 min; take the supern...

Embodiment 3

[0068] Embodiment three: aspergillus ( Aspergillus sp. ) Radiation resistance of F77CGMCC No. 8382

[0069] Two conditions must be met for microorganisms to treat radioactive pollution in the environment, that is, they can survive in a radiation environment and have high adsorption selectivity for polluting nuclides. The fungus Aspergillus F77 used was extracted from radionuclide-contaminated soil. 60 Co radioactive source γ-ray irradiation, the cumulative radiation dose is 10 kGy. By comparing the culture of irradiated samples and non-irradiated samples, it was found that its growth was not affected by irradiation, that is, Aspergillus F77 has a strong ability to resist radiation. The radiation characteristics (γ radiation) of F77 are based on Ferreira et al. [9] Established methods were validated. Bacteria were cultured in PDA liquid medium to the stable stage, centrifuged at 4°C, washed with normal saline, and the suspension concentration in normal saline was control...

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Abstract

The invention discloses a radiation-resistant Aspergillus sp. and application thereof in cesium 137 adsorption biological treatment. Sampling in a certain region, screening, separation, screening and physiologic and biochemical identification are performed to obtain the Aspergillus sp. F77 CGMCC No.8382. By utilizing the application of the Aspergillus sp. in cesium 137 adsorption biological treatment, the Aspergillus sp. has the maximal resistance concentrations for Pb<2+>, Zn<2+> and Ni<+>, which can respectively reach 1000 mg / L, 500 mg / L and 500 mg / L; and the resistance concentrations for Co<2+>, Cr<2+> and Hg<2+> take second place, and can respectively reach 200 mg / L. According to the method, the growing thallus can adsorb caesium ions and radiocesium, or the dry thallus can be directly used for adsorption, wherein the maximal adsorption capacity can reach 44.5 mg / g dry thallus. The application of the strain has actual value and functions in Cs 137 adsorption biological treatment.

Description

technical field [0001] The invention relates to the technical field of a microbial strain applied to bioabsorption of radionuclides. Specifically, the invention relates to a radioresistant Aspergillus and the technical field of its application in radionuclide adsorption. Background technique [0002] Urbanization, industrialization and population growth have caused a global energy crisis, and the need for sustainable development has made human beings more and more dependent on new energy. Nuclear energy has attracted more and more attention from many countries because of its extremely low carbon dioxide emission and huge development potential. The rapid development of nuclear energy has also brought enormous pressure to the global environment, making radioactive pollution one of the current major environmental problems, especially the nuclear accidents represented by Chernobyl and Fukushima nuclear power plants in Japan released a large amount of Radioactive pollutants caus...

Claims

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Application Information

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IPC IPC(8): C12N1/14B01J20/00C12R1/66
Inventor 王玮王卫宪张志东谢玉清张丽娟徐辉顾美英朱静唐琦勇宋素琴王耀琴楚敏
Owner THE INST OF MICROBIOLOGY XINJIANG ACADEMY OF AGRI SCI
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