The invention discloses a radiation-resistant Aspergillus sp. and application thereof in cesium 137 adsorption biological treatment. Sampling in a certain region, screening, separation, screening and physiologic and biochemical identification are performed to obtain the Aspergillus sp. F77 CGMCC No.8382. By utilizing the application of the Aspergillus sp. in cesium 137 adsorption biological treatment, the Aspergillus sp. has the maximal resistance concentrations for Pb<2+>, Zn<2+> and Ni<+>, which can respectively reach 1000 mg/L, 500 mg/L and 500 mg/L; and the resistance concentrations for Co<2+>, Cr<2+> and Hg<2+> take second place, and can respectively reach 200 mg/L. According to the method, the growing thallus can adsorb caesium ions and radiocesium, or the dry thallus can be directly used for adsorption, wherein the maximal adsorption capacity can reach 44.5 mg/g dry thallus. The application of the strain has actual value and functions in Cs 137 adsorption biological treatment.