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Assimilated nitrate nitrogen strain and application thereof

A technology of nitrate nitrogen and bacteria strains, applied in the direction of application, bacteria, chemical instruments and methods, etc., can solve the problems of high cost of soil amendment method, loss of soil nitrogen, pollution of groundwater, etc., and achieve easy industrialization implementation and improvement Utilization rate, the effect of high tolerance concentration

Inactive Publication Date: 2011-10-19
SHANGHAI JIAO TONG UNIV
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  • Description
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AI Technical Summary

Problems solved by technology

At present, some measures have been taken to deal with the salinization hazards of protected cultivation greenhouses in my country, such as the method of irrigation and salt washing, the method of soil improver and the method of semi-decomposed organic fertilizer. Although these methods have many advantages, they also have great shortcomings. : The irrigation and salt washing method makes the nitrate nitrogen leached into the groundwater, which not only causes the loss of soil nitrogen, but also pollutes the groundwater; the cost of the soil amendment method is relatively high, and it may cause secondary pollution; semi-decomposed organic fertilizer Slow fertilizer effect, inconvenient use and other problems
As a result the gas N 2 O, have adverse effects on the environment

Method used

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  • Assimilated nitrate nitrogen strain and application thereof
  • Assimilated nitrate nitrogen strain and application thereof
  • Assimilated nitrate nitrogen strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Embodiment 1, the isolation and purification of Bacillus megaterium (Bacillus megaterium)

[0028] Soil samples were collected from the greenhouse soil of 12 to 15 years of facility cultivation in Chongming Island. The shake flask enrichment culture method was used. After mixing different soil samples evenly, 10g was taken, placed in 100mL enrichment medium, and placed at 25°C, 120r / h Min was cultured on a constant temperature shaker, and then transplanted every 7 days. Afterwards, 10% of the inoculum amount was inserted into the enrichment medium, and acclimatization was repeated four times. The enrichment medium adopts inorganic salt liquid medium.

[0029] Take 100 μL of the suspension of the domesticated bacteria and spread it on the inorganic salt solid agar plate, place it in a 25°C constant temperature and humidity incubator and cultivate it upside down. Obtain a single colony, and then pass the obtained single colony on the inorganic salt solid agar medium wit...

Embodiment 2

[0031] The bacterial strain obtained in Example 1 was inserted into the nitrate nitrogen concentration of 10% of the inoculum to simulate the soil to be treated in the liquid inorganic salt medium of 200mk / kg, and 120r / min shaking culture at 25°C for 1-5 sky.

[0032] The bacterial strain refers to: the NCT-2 assimilating nitrate nitrogen bacteria in the strain preservation environment is subjected to the strain activation treatment and the strain cultivation treatment in sequence.

[0033] The preservation environment of the strains refers to: adding glycerin as a protective agent in a low-temperature refrigerator at -70°C for cryopreservation, keeping the microorganisms in a frozen state, and stopping their metabolism to achieve the purpose of preservation.

[0034] The strain activation treatment refers to: put the NCT-2 assimilating nitrate nitrogen bacteria under the strain preservation environment into the PDA medium for cultivation, so that the strains can gradually ada...

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Abstract

The invention relates to the technical field of microorganism soil processing, in particular to an assimilated nitrate nitrogen strain and application thereof. The strain is NCT-2 assimilated nitrate nitrogen strain, the classification name is Bacillus megaterium, the strain is preserved in the Chinese General Microbiological Culture Collection Center, the preservation date is March 21, 2011, and the preservation number is CGMCC No.4698. The invention can effectively assimilate the microorganisms of excessive nitrate nitrogen in facility cultivation soil.

Description

technical field [0001] The invention relates to a bacterium in the technical field of microbial soil treatment and an application method thereof, in particular to a nitrate nitrogen assimilating bacterium and an application thereof. Background technique [0002] With the expansion of facility cultivation area, the use of nitrogen fertilizers has increased significantly, which greatly exceeds the demand of plants. Since the nitrate absorbed by plants cannot be reduced and assimilated in time, the accumulation in plants is becoming more and more serious. At the same time, a large amount of nitrogen fertilizers that plants cannot absorb The salt in the soil accumulates in the soil, causing secondary salinization of the soil, in which the accumulation of nitrate is the main one. [0003] From an environmental point of view, the accumulation of N elements can cause waste of fertilizers and affect crop growth and soil utilization. At the same time, it can cause agricultural non-po...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C09K17/00C12R1/11C09K101/00
Inventor 周培支月娥刘群录时唯伟张春华
Owner SHANGHAI JIAO TONG UNIV
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