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Delftia tsuruhatensis LW26 and application thereof in chlorobenzene degradation

A technology of Delftia, LW26, which is applied in the direction of bacteria, microorganism-based methods, water/sludge/sewage treatment, etc., can solve the problem that Delftia has not been seen to degrade chlorobenzene, etc., and achieves fast degradation speed and tolerance concentration high effect

Active Publication Date: 2015-11-11
ZHEJIANG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] After searching the relevant literature, there is no report on the degradation of chlorobenzene with Delftia bacteria

Method used

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  • Delftia tsuruhatensis LW26 and application thereof in chlorobenzene degradation
  • Delftia tsuruhatensis LW26 and application thereof in chlorobenzene degradation
  • Delftia tsuruhatensis LW26 and application thereof in chlorobenzene degradation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1: Isolation, purification and identification of Delftia LW26

[0033] (1) Isolation and purification of Delftia LW26

[0034] The biofilm on the packing surface of the bio-trickling filter tower was collected. This filter tower is a bio-trickling filter tower for the treatment of chlorobenzene waste gas in the Biotransformation and Bio-purification Laboratory of Zhejiang University of Technology. The inoculated activated sludge came from the sewage treatment tank of a pharmaceutical factory in Zhejiang. Stable operation for 5 months. After the biofilm was isolated and purified, a strain that could efficiently degrade chlorobenzene was obtained. Specific steps are as follows:

[0035] Take 5g filler from the biotrickling filter tower, wash the biofilm with 30mL sterile water in a shaker flask, place on a shaker at 160rpm, and shake at 30°C for 1 hour. Then use the inorganic salt medium containing chlorobenzene (the initial concentration of chlorobenzene is 10...

Embodiment 2

[0043] Embodiment 2: the preparation of Delftia LW26 bacterial suspension

[0044] When the amount of bacterial solution is small, the Delftia LW26 bacterial suspension can be obtained through three steps of inclined plane culture, seed culture and bacterial suspension preparation; The four steps of culture and bacterial suspension preparation are obtained, and the specific process is as follows:

[0045] (1) Slant culture: inoculate Delftia LW26 on R 2 A solid medium, cultivated at 28-32°C for 48 hours, to obtain slant bacteria;

[0046](2) Seed culture: pick an inoculation loop colony from the slope of step (1) and inoculate it into the seed medium, cultivate at 28-32°C for 24-36 hours, and obtain the seed liquid;

[0047] (3) Fermentation culture: inoculate the seed solution obtained in step (2) to the fermentation medium with an inoculum volume concentration of 5-10%, and cultivate it for 24-36 hours at 28-32°C and pH 6.0-8.0 to obtain fermentation broth;

[0048] (4) ...

Embodiment 3

[0049] Embodiment 3: Degradation characteristics of Delftia LW26 p-chlorobenzene

[0050] (1) Effect of temperature on the growth of Delftia LW26 and the degradation of chlorobenzene

[0051] The degradation experiment of Delft LW26 p-chlorobenzene was carried out at different temperatures, and it was found that it has a high ability to degrade chlorobenzene at 23-30 ° C. The specific experimental scheme is as follows:

[0052] Add 50mL inorganic salt culture medium to a 300mL shake flask, pH value 7.0, after sterilizing at 110°C for 40 minutes, add chlorobenzene as the only carbon source, so that the initial concentration of chlorobenzene is 100mg / L, then add 1mL of Example 2 Methods Delftia LW26 bacterial suspension (OD 600 = 0.15). During the experiment, three parallel samples and one blank control (without adding LW26 bacterial suspension) were designed for each temperature. Place each sample in a shaker at 23°C, 25°C, 30°C, 35°C, and 40°C for constant temperature culti...

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Abstract

The invention provides a strain Delftia tsuruhatensis LW26 which is capable of efficiently degrading chlorobenzene and application thereof in microbial decomposition and treatment of chlorobenzene. The Delftia tsuruhatensis LW26 is preserved in China Center for Type Culture Collection (CCTCC): Wuhan University, Wuhan, China, 430072, the preservation date is March 15, 2015 and a preservation number is CCTCC NO. M2015113. The chlorobenzene degradation bacteria provided by the invention can use chlorobenzene as a unique carbon source and energy for propagation and completely mineralize the chlorobenzene into CO2 and H2O; the strain can effectively degrade chlorobenzene in an environment of 23-30 DEG C and pH of 6.0-9.0; the strain has higher environment suitability and can play an important role in practice of waste gas and waste water treatment.

Description

(1) Technical field [0001] The invention relates to a novel chlorobenzene efficient degrading bacterium - Delftiatsuruhatensis LW26 and its application. (2) Background technology [0002] Chlorobenzene is a monocyclic aromatic compound with only hydrogen and chlorine atoms on the benzene ring, which has the characteristics of low solubility and strong hydrophobicity. In recent years, as an important organic solvent and intermediate in chemical production, chlorobenzene has been widely used in plastics, dyes, medicine, pesticides, organic synthesis and other industries. Due to its low water solubility and high biological toxicity, it causes serious pollution to the environment. After entering the human body, chlorobenzene can inhibit the nerve center, easily combine with the enzyme system, and have teratogenic and carcinogenic effects. Due to its wide use, chlorobenzene has a high detection rate in various environmental media, which poses a certain threat to human health an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34B01D53/84B01D53/70C12R1/01C02F101/36
CPCY02A50/20Y02P20/59
Inventor 陈建孟叶杰旭陈东之李伟林彤晖诸葛蕾江宁馨
Owner ZHEJIANG UNIV OF TECH
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