Deep-sea microbacterium with tolerance to vanadium and use thereof

A deep-sea microbacteria, a technology for use, applied in the field of microorganisms, to achieve the effects of high tolerance, fast growth speed, and high tolerance concentration

Active Publication Date: 2019-04-30
CHENGDU UNIVERSITY OF TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The technical problem to be solved by the present invention is to provide a technical solution for the bioremediation of the environment polluted by heavy metals

Method used

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  • Deep-sea microbacterium with tolerance to vanadium and use thereof
  • Deep-sea microbacterium with tolerance to vanadium and use thereof
  • Deep-sea microbacterium with tolerance to vanadium and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] The acquisition and separation of embodiment 1 bacteria

[0026] 1. The process of separating and purifying bacteria:

[0027] (1) Take vanadium-contaminated water in the drainage channel of the vanadium-titanium-magnetite west slag yard in Panzhihua City, Sichuan, Sichuan, and let it stand for 2 hours. Take 1 mL of supernatant and add it to 50 mL of liquid medium containing heavy metal vanadium at 10 mg / L, at 30°C. 120rpm culture for 4d;

[0028] (2) Take 1 mL of the liquid culture medium cultured for 4 days in (1) and add it to 50 mL of the liquid culture medium containing heavy metal vanadium at 50 mg / L, and culture at 30°C and 120 rpm for 4 days;

[0029] (3) Take 1 mL of the liquid culture medium cultured for 4 days in (2) and add it to 50 mL of the liquid culture medium containing heavy metal vanadium at 100 mg / L, and culture at 30°C and 120 rpm for 4 days;

[0030] (4) Take 1 mL of the liquid culture medium cultured for 4 days in (3) and add it to 50 mL of the ...

Embodiment 2

[0037] Example 2 Bacterial morphological characteristics and biological identification

[0038] 1. Morphological characteristics

[0039] The monoclonal colonies obtained in Example 1 were picked and streaked on LB solid medium, and the plate was placed upside down in a constant temperature incubator, incubated at 30° C. for 24 hours, and the colony morphology was observed. Exiguobacterium profundum PFWT01 forms an irregular circle with a smooth and moist surface, slightly raised in the center of the colony, irregular edges, soft texture, orange-yellow, non-proliferating, see figure 1 .

[0040] 2. Physiological and biochemical properties

[0041] The monoclonal colony obtained in Example 1 was inoculated into the LB liquid basal medium, and the Exiguobacterium profundum PFWT01 strain in the logarithmic growth phase was used to carry out Gram staining, starch staining, Hydrolysis test, oil hydrolysis test, methyl red test, H 2 S experiment, gelatin hydrolysis experiment, s...

Embodiment 3

[0049] Example 3: Maximum tolerated concentration of bacteria against heavy metals

[0050] Pick the monoclonal colony obtained in Example 1, inoculate it in LB liquid medium, shake and culture at 30°C and 120rpm for 36h, and collect the bacterial concentration OD. 600 As 1.2, the bacterial solution was diluted according to the ratio of 1, 10, 100, 1 000, 10 000 to prepare gradient bacterial solution; and LB solid medium for heavy metals with different concentrations was prepared. Draw 50 μL of each concentration of bacteria solution to various heavy metal concentrations and spread evenly on the plate, cultivate at 30 °C, observe and record the growth of bacteria every 24 hours, and the minimum heavy metal concentration that inhibits its obvious growth within 120 hours is the maximum tolerance of the bacteria. concentration.

[0051] The above-mentioned heavy metal solid medium with different concentrations is LB solid medium supplemented with Cr, Pb, Cu, Ni, Co, Cd, Zn or V,...

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Abstract

The invention belongs to the field of microbes, and in particular relates to a deep-sea microbacterium resistant to vanadium and its application. The technical problem to be solved by the invention is to provide a technical solution for the bioremediation of the environment polluted by heavy metals. The technical solution of the present invention to solve the technical problem is to provide a deep-sea microbacterium Exiguobacterium profundum PFWT01, and its preservation number is CCTCC NO: M2016175. In addition to being highly resistant to vanadium, the bacterium has tolerance to heavy metals such as Cr, Pb, Cu, Ni, Co, etc., and its tolerance to vanadium is as high as 2000mg / L; The ability to reduce to tetravalent vanadium, with a conversion rate of 54.33%, can reduce the toxicity of high-valent vanadium in the solution, especially for the removal of vanadium toxicity in high-vanadium-toxic liquid environments polluted by other heavy metals. It has a good application prospect in restoration.

Description

technical field [0001] The invention belongs to the field of microorganisms, and in particular relates to a deep-sea microbacterium with tolerance to vanadium and use thereof. Background technique [0002] Heavy metal pollution is becoming more and more serious. With the discharge of a large amount of industrial waste, agricultural waste water and domestic sewage, heavy metal pollutants are also discharged together, causing heavy metal pollution in water. After heavy metals are discharged into the environment, they are not easily degraded and can accumulate in the environment for a long time. Improper handling of heavy metals will lead to the release of a large number of potentially carcinogenic and mutagenic toxic compounds, resulting in heavy metal pollution that is very harmful to organisms and the environment. Therefore, it is crucial to strengthen heavy metal pollution control and improve human health and ecosystem security. [0003] Vanadium is a silver-gray metal wit...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/01C02F101/20
CPCC02F3/34C02F2101/20C12N1/205C12R2001/01
Inventor 彭书明黄艺李迅倪师军阳小成谢鸿观王杰
Owner CHENGDU UNIVERSITY OF TECHNOLOGY
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