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Bacillus separated from soil and application thereof

A bacillus and soil technology, applied in the field of microorganisms, can solve the problem of low vanadium reduction rate and achieve the effect of simple operation process

Active Publication Date: 2022-04-08
CHENGDU UNIVERSITY OF TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The existing published bacteria are rarely able to reduce vanadium and chromium at the same time, and the reduction rate of vanadium is not high, and further improvement is needed

Method used

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  • Bacillus separated from soil and application thereof
  • Bacillus separated from soil and application thereof
  • Bacillus separated from soil and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1 Separation, purification and identification of Bacillus pacificus SM02

[0039] Select the heavily polluted vanadium and chromium area near the Panzhihua vanadium-titanium magnetite smelter. According to the test results, the content of vanadium and chromium in the local soil can reach up to 4156.47mg·kg -1 and 1377.15mg·kg -1 .

[0040] 1. Isolation and purification of bacteria

[0041] (1) Select 1g of soil from the polluted area and add it to a conical flask containing 99mL of sterilized deionized water. After shaking at 150rpm at 38°C for two hours, take 1mL of the supernatant and add it to 9mL of sterile water, mix well, and repeat the above steps , respectively to obtain a concentration of 10 of the stock solution -1 , 10 -2 , 10 -3 , 10 -4 , 10 -5 , take 10 -3 , 10 -4 , 10 -5 The concentration of the original solution was spread into LB solid medium, and incubated at a constant temperature of 30°C for 24h.

[0042] (2) Select the single strai...

Embodiment 2

[0054] Embodiment 2 tolerance concentration test

[0055] Bacillus pacificus SM02 was inoculated to a vanadium concentration of 1000 mg L by continuous streaking. -1 , 1100mg·L -1 , 1200mg·L -1 , 1300mg·L -1 , the concentration of chromium is 900mg·L -1 , 1000mg·L -1 , 1100mg·L -1 , 1200mg·L -1 Incubate in solid medium at 30°C for 24 hours. The final concentration of vanadium and chromium is 1200mg·L -1 and 1000mg·L -1 Bacterial growth gradually decreased. It shows that the tolerance concentration of this strain of bacteria to vanadium and chromium respectively reaches 1200mg·L -1 and 1000mg·L -1 .

Embodiment 3

[0056] Embodiment 3 reduction effect test

[0057] Inoculate Bacillus pacificus SM02 into LB liquid medium and expand culture for 20h at 30°C 150rpm, then wash the bacteria twice with sterile water to adjust the OD 600 is 1. According to 1% inoculum amount, add to contain 50mg·L -1 V(Ⅴ) and 50mg·L -1 Cr(Ⅵ) solution, and added citric acid as the electron donor, the dosage of citric acid was 6mM, 38℃150rpm constant temperature culture for 24h, 4000rpm centrifugation, the supernatant was taken and filtered, and measured by UV spectrophotometry. Pentavalent vanadium and hexavalent chromium concentration, ICP-OES determination of total vanadium and total chromium content. At the same time, set the inactivated bacteria group as a control to determine whether the bacteria have adsorption effect. The results are shown in Table 1:

[0058] Table 1

[0059]

[0060] Among them, "-" indicates that there is no data, because the concentration measured by spectrophotometry is not a...

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Abstract

The invention relates to bacillus separated from soil and application thereof, and belongs to the technical field of microorganisms. The first technical problem solved by the invention is to provide the bacillus separated from the soil. The bacterium is bacillus pacificus SM02 separated from soil, and is preserved in the China Center for Type Culture Collection on August 16, 2021, and the preservation number of the bacterium is CCTCC NO: M 20211039. The Bacillus pacificus SM02 has high tolerance to vanadium and chromium in the environment, the tolerance concentration of the Bacillus pacificus SM02 to vanadium and chromium reaches up to 1200 mg.L <-1 > and 1000 mg.L <-1 >, the Bacillus pacificus SM02 can adapt to the survival condition of a high background pollution area, the bacterium can completely reduce hexavalent chromium, the reduction rate of 98.43 + / -0.42% to pentavalent vanadium is achieved, the reduction rate is high, the operation process is simple, convenient and efficient, and the method is suitable for industrial production. And a foundation is laid for a vanadium and chromium pollution bioremediation technology.

Description

technical field [0001] The invention relates to bacillus isolated from soil and application thereof, belonging to the technical field of microorganisms. Background technique [0002] China is rich in mineral resources. Although mineral resources play an important role in promoting social and economic development, heavy metals and other harmful substances will be released during the development of these resources, thereby damaging the ecological environment. Excessive levels of heavy metals in soil and water can reduce soil productivity and quality, affecting plant growth on the one hand, and microbial community diversity, metabolic potential, and functional cycles of associated ecosystems on the other. Heavy metals exist in the environment for a long time, and these heavy metals will be enriched in the human body through the food chain and eventually threaten human health. For example, acute inhalation of vanadium may cause bronchopneumonia, and chronic inhalation will caus...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34B09C1/08C12R1/07C02F101/20C02F101/22
Inventor 黄艺唐雪李瑛张佩聪周丹何鹏张文涛张若曦
Owner CHENGDU UNIVERSITY OF TECHNOLOGY
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