Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Radiation-proof Fusarium sp. and application thereof to cesium adsorption biotreatment

A technology of biological treatment and Fusarium, applied to radiation-resistant Fusarium and its application in the adsorption of radionuclide cesium, microbial strains are applied to the field of bioabsorption of radionuclides, which can solve the limitation of the ability to repair radioactively polluted environments And other issues

Inactive Publication Date: 2015-03-11
THE INST OF MICROBIOLOGY XINJIANG ACADEMY OF AGRI SCI
View PDF1 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since most bacteria are sensitive to radioactivity, their ability to remediate radioactively contaminated environments is limited
There are no reports on radioresistant Fusarium and its application in radionuclide adsorption

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Radiation-proof Fusarium sp. and application thereof to cesium adsorption biotreatment
  • Radiation-proof Fusarium sp. and application thereof to cesium adsorption biotreatment
  • Radiation-proof Fusarium sp. and application thereof to cesium adsorption biotreatment

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Embodiment one: Fusarium ( Fusarium sp. .) Isolation and cultivation of F54 CGMCC No.8359

[0037] 1. Separation

[0038] Radiation resistant Fusarium used in the present invention ( Fusarium sp. .) The Institute of Microbiology Application of Xinjiang Academy of Agricultural Sciences took samples from radioactively contaminated soil in a certain place in Xinjiang, and used the traditional plate culture method to separate the microorganisms in the soil layer. The culture medium is an enrichment condition, and a batch of well-growing microbial strains are screened out, and a strain numbered F54 is selected out of them.

[0039] Separation steps: According to the gradient dilution method, weigh 10g of soil samples in 90mL sterile physiological saline, and perform gradient dilution after activation at 30°C for 30min. Select 10 -2 、10 -3 、10 -4 The dilutions were spread on the plates of PDA medium respectively, and each treatment was replicated three times, and cul...

Embodiment 2

[0049] Embodiment two: Fusarium ( Fusarium sp. ) Molecular level identification of F54 CGMCC No.8359

[0050] 1. Extraction of fungal genome:

[0051] Pick fungal hyphae from the culture medium and remove agar as much as possible; add 200ul plant lysis buffer, grind to powder; add 600ul plant lysis buf, 0.8ul β-mercaptoethanol, mix well; 65℃ water bath for 40min, 12000rpm, 10min Centrifuge; remove the supernatant, add an equal volume of chloroform to extract, centrifuge at 12000rpm for 5min; add 700ul plant Binding buf, put it on the column for 5min; centrifuge at 12000rpm for 2min; add 500ul 80% ethanol to wash twice, centrifuge at 12000rpm for 1min; room temperature Let stand for 10min to evaporate and dry, add 40ul H2O, let stand for 5-10min; replace with a new tube, centrifuge at 13000rpm for 2min, the bottom of the tube is the genome.

[0052] 2. ITS sequence amplification and sequencing

[0053] Amplification with fungal ITS universal primers:

[0054] Forward pri...

Embodiment 3

[0059] Embodiment three: Fusarium ( Fusarium sp. .) Radiation resistance of F54 CGMCC No.8359

[0060] Two conditions must be met for microorganisms to treat radioactive pollution in the environment, that is, they can survive in a radiation environment and have high adsorption selectivity for polluting nuclides. The fungus Fusarium sp. ( Fusarium sp. ) F54 is extracted from radionuclide-contaminated soil. 60 Co radioactive source γ-ray irradiation, the cumulative radiation dose is 10 kGy. Through the comparison of irradiated samples and non-irradiated samples, it was found that its growth was not affected by irradiation, that is, Fusarium sp. F54 had a strong ability to resist radiation.

[0061] Show that Fusarium provided by the invention ( Fusarium sp. ) F54 CGMCC No.8359 was isolated from radioactive soil and accumulated with an irradiation dose of 10 kGy, which has strong radiation resistance.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses radiation-proof Fusarium sp. and application thereof to cesium adsorption biotreatment. Through providing of the self-screening radiation-proof Fusarium sp. F54 CGMCC No. 8359 and application thereof, tests show that the Fusarium sp. F54 CGMCC No. 8359 has tolerance to various metals, and has the maximal tolerance concentrations to Pb <2+>, Zn <2+> and Ni <2+>, which can respectively reach 1000 mg / L, 500 mg / L and 500 mg / L, and the tolerance to Co <2+>, Cr<2+> and Hg<2+> take second place and can reach 200 mg / L. When the radiation-proof Fusarium sp. strain is applied to cesium adsorption, remarkably obvious technical effects are achieved, and accordingly, a favorable application prospect of the radiation-proof Fusarium sp. in cesium adsorption biotreatment is testified.

Description

technical field [0001] The invention relates to the technical field of a microbial strain applied to bioabsorption of radionuclides. Specifically, the invention relates to a radioresistant Fusarium fungus and the technical field of its application in radionuclide cesium adsorption. Background technique [0002] Urbanization, industrialization and population growth have caused a global energy crisis, and the need for sustainable development has made human beings more and more dependent on new energy. Nuclear energy has attracted more and more attention from many countries because of its extremely low carbon dioxide emission and huge development potential. The rapid development of nuclear energy has also brought enormous pressure to the global environment, making radioactive pollution one of the current major environmental problems, especially the nuclear accidents represented by Chernobyl and Fukushima nuclear power plants in Japan released a large amount of Radioactivity, c...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14G21F9/12C12R1/77
CPCC12N1/145C12R2001/77G21F9/12
Inventor 张丽娟王玮张志东谢玉清韩小元赖成霞唐琦勇朱静楚敏
Owner THE INST OF MICROBIOLOGY XINJIANG ACADEMY OF AGRI SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com