A rotor
assembly (10, 150, 270, 310) and method of using the rotor
assembly (10, 150, 270, 310). The rotor
assembly (10, 150, 270, 310) includes a bio-process bag (48), a drum (46) that receives a lower portion of the bag (48), and a pressure ring (50). A holder (54, 182) couples an upper portion of the bag (48) to the pressure ring (50). The pressure ring (50) is coupled to the drum (46) to define an
interior space that contains the bag (48). A liquid transport assembly (35, 178, 272) passes through an opening in the holder (54, 182) so that liquids can be added to, and removed from, the bag (48) without removing the rotor (16, 154) from the
centrifuge. A bearing assembly (190) in the holder (54, 182) couples the liquid transport assembly (35, 178, 272) to the rotor (16, 154), and enables the liquid transport assembly (35, 178, 272) to remain stationary while the rotor (16, 154) rotates around it. One or more seal assemblies (276, 312) provide a fluid-tight seal against the outer portion of the liquid transport assembly (35, 178, 272), and prevent fluids from leaking from the bag (48) during
centrifugation.