The invention discloses a method for culturing a microbial flora for purifying waste water, which comprises the following steps: A, filtering, settling and concentrating active sludge, adding coking wastewater at the temperature of below 33 DEG C, adding nutrients, proteins and microbial growth hormone at the same time, aerating for 3 to 5 days under a constant-temperature condition, settling, discharging supernatant, adding glucose solution and domesticating according to the operation mode of a batch reactor activated sludge process; B, at the end of domestication, screening cocci, bacilli and spirilla, placing into containers for low-temperature storage respectively, injecting 10 percent glucose solution into the containers for repair, and taking the bacterial strains out 24 hours later for mixing; and C, performing aerobic and anaerobic culture of the mixed bacterial strains, and adding a stabilizer and strain protective agent. For common active sludge, the method can reduce the phenol, cyanogens, COD and ammonia nitrogen content of coking wastewater more effectively and reduce sludge discharge obviously.