Method for culturing microbial flora for purifying waste water

A microbial flora and wastewater purification technology, applied in biochemical equipment and methods, microorganisms, microorganisms, etc., can solve the problems of enzyme system poisoning, treatment effect decline, microbial adsorption performance decline, etc., and achieve the effect of reducing sludge discharge

Inactive Publication Date: 2010-12-15
WUHAN IRON & STEEL (GROUP) CORP
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Problems solved by technology

However, there are many problems in the general microbial treatment: (1) When the concentration of ammonia nitrogen in the wastewater is high, especially when there is free ammonia, the microbial adsorption performance will decrease, resulting in NO in the wastewater. 2 - accumulation
(2) Microorganisms have poor impact resistance, and are susceptible to the impact of toxic organic substances

Method used

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  • Method for culturing microbial flora for purifying waste water

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Embodiment

[0014] After filtering, settling and concentrating the activated sludge from the underground drain of the coking plant, add 1L of coking wastewater, its COD is 6000mg / L, the temperature is 30℃, the NH3-N content is 500mg / L, and the phenol content is 550mg / L, the cyanide content is 40mg / L; at the same time, add glucose, nitrogen, and disodium hydrogen phosphate in a ratio of 100:5:1, aerate for 4 days under constant temperature conditions, discharge the supernatant after sedimentation, and add glucose solution According to the operation mode of the intermittent activated sludge method, the concentration of DO (dissolved oxygen) is controlled at 4mg / L. After 24 hours of aeration, the sedimentation ratio is detected for half an hour. After the sedimentation ratio has increased to a certain extent, 1L of coking wastewater is added. At the same time, glucose, nitrogen, and disodium hydrogen phosphate were added in a ratio of 100:5:1, and aeration was carried out for 4 days under con...

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Abstract

The invention discloses a method for culturing a microbial flora for purifying waste water, which comprises the following steps: A, filtering, settling and concentrating active sludge, adding coking wastewater at the temperature of below 33 DEG C, adding nutrients, proteins and microbial growth hormone at the same time, aerating for 3 to 5 days under a constant-temperature condition, settling, discharging supernatant, adding glucose solution and domesticating according to the operation mode of a batch reactor activated sludge process; B, at the end of domestication, screening cocci, bacilli and spirilla, placing into containers for low-temperature storage respectively, injecting 10 percent glucose solution into the containers for repair, and taking the bacterial strains out 24 hours later for mixing; and C, performing aerobic and anaerobic culture of the mixed bacterial strains, and adding a stabilizer and strain protective agent. For common active sludge, the method can reduce the phenol, cyanogens, COD and ammonia nitrogen content of coking wastewater more effectively and reduce sludge discharge obviously.

Description

technical field [0001] The invention relates to a method for cultivating microbial flora, in particular to a method for cultivating microbial flora used in wastewater purification treatment. Background technique [0002] Coking wastewater is formed by the escape and condensation of coal gas during the high-temperature dry distillation process. The pollutants in it are of various types and complex components, which are extremely harmful and difficult to deal with. Due to the advantages of large processing capacity, high purification rate, low cost, and no secondary pollution, microbial treatment of wastewater has been widely used in the field of sewage treatment. However, there are many problems in the general microbial treatment: (1) When the concentration of ammonia nitrogen in the wastewater is high, especially when there is free ammonia, the microbial adsorption performance will decrease, resulting in NO in the wastewater. 2 - accumulation. (2) Microorganisms have poor...

Claims

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Application Information

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IPC IPC(8): C12N1/00C12N1/20C02F3/34
Inventor 魏松波盛军波常红兵段爱民何一兵肖建军王凯军刘刚
Owner WUHAN IRON & STEEL (GROUP) CORP
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