99 results about "Isophorone" patented technology

The invention discloses an application of rhodococcus diploides in treatment of waste water containing isophorone, the rhodococcus diploides is classified and named as Rhodococcus tectodiphilus, the strain number is GXGD-YZ-2510, and the preservation number is GDMCC No: 67304. The invention further discloses a preparation method of the rhodococcus diploides. The strain shows good growth metabolism activity and isophorone degradation efficiency in a high-salt aerobic environment. Under the conditions that the system salinity is 1 wt%, the initial isophorone concentration is 200 mg / L, and the dissolved oxygen is 3-5 mg / L, the removal rate of the strain on the isophorone reaches 78.6%. The strain not only has excellent salt stress resistance, but also can realize efficient bioconversion of isophorone under high-salt aerobic conditions, and effectively solves the technical bottleneck that isophorone in high-salt wastewater in the industries of petrochemical engineering, chemical engineering, pharmacy and the like is difficult to biochemically treat; the method has the remarkable advantages of being thorough in degradation, low in cost, environmentally friendly, high in process adaptability and the like, and a novel strain resource and an industrialization technical scheme are provided for biological treatment of high-salinity wastewater of refractory organic pollutants.

The invention discloses an alcohol dehydrogenase mutant for high yield of tea flavor ketone and application of the alcohol dehydrogenase mutant in a one-pot method. The mutant is A182L or A182L / V214T. The alcohol dehydrogenase mutant has high catalytic activity on 4-hydroxyisophorone, and the yield of tea flavor ketone prepared by catalysis of the mutant A182L / V214T can reach 97% and is 3.73 times that of an original enzyme; the alcohol dehydrogenase mutant has high catalytic activity of synthesizing the tea aroma ketone through one-pot catalysis of isophorone, and the yield of the tea aroma ketone obtained through direct oxidation of isophorone by jointly using the mutant A182L / V214T and a cytochrome P450BM3 monooxygenase mutant can reach 92%; the alcohol dehydrogenase mutant has the advantages of simple production process, mild reaction conditions and environment-friendly production process, is beneficial to the industrial production of the tea flavor ketone, and has wide application prospects.

The invention discloses an anti-fouling and anti-corrosion integrated coating with long-acting, high-adhesion and super-smooth functions as well as a preparation method and application of the anti-fouling and anti-corrosion integrated coating, and belongs to the field of preparation of marine anti-fouling and super-smooth coating materials. The preparation method comprises the following steps: preparing a high-adhesion epoxy resin precursor to obtain a liquid product EGDE-PCA; the preparation method comprises the following steps: preparing a high-adhesion and super-lubricity integrated epoxy resin precursor to obtain an oily liquid product EGDE-PCA / APT-PDMS; the preparation method comprises the following steps: adding EGDE-PCA / APT-PDMS into isophorone diamine, uniformly mixing and stirring, coating a metal material sample with the mixture, and carrying out a drying and curing reaction under a vacuum heat preservation condition, so as to obtain the antifouling and anticorrosive integrated coating with long-acting high-adhesion and super-lubricity functions. In the early-stage synthesis reaction, an organic solvent does not need to be added, so that the environmental protection property is improved, and the problem that the construction process is difficult due to poor flowability is solved; the coating provided by the invention has super-smooth durability and excellent antifouling and anticorrosion effects.

A process for the manufacture of isophorone diamine (IPDA), comprising the steps of:a) providing a feed stream comprising trans-IPDA, cis-IPDA, isophorone nitrile amine (IPNA), components having a lower boiling point than trans-IPDA and components having a higher boiling point than IPNA, including isophorone amino alcohol (IPAA) and components having a higher boiling point than IPAA;b) separating the feed stream into(i) a fraction (ii) comprising a higher mass fraction of cis-IPDA content, compared to the feed stream;(ii) a fraction (iii) comprising a higher mass fraction of IPNA compared to the feed stream and a higher mass fraction of components having a boiling point higher than the boiling point of IPNA, including IPAA and components having a higher boiling point than IPAA, compared to the feed stream and optionally IPDA;c) further separating fraction (iii) into(iii) a fraction (iii-1) comprising a higher mass fraction of IPDA, compared to fraction (iii); and / or(iv) a fraction (iii-3) comprising a higher mass fraction of IPAA, compared to fraction (iii).

The invention discloses an anti-UV hydrolysis-resistant PET functional master batch and a preparation method thereof, UV absorbing groups such as isophorone diester and 2, 4-dihydroxybenzoic acid are introduced to enhance the UV resistance, and neopentyl glycol and trimethylolpropane are introduced to enhance the hydrolysis resistance of a molecular chain. Besides, the Beta molecular sieve, the zinc metaborate and other auxiliaries are utilized, so that the uniform distribution of the light stabilizer and the ultraviolet light absorber in the molecular structure is improved, and the problem that the UV absorption performance and the hydrolysis resistance of the film are not uniform due to molecular arrangement change in the stretching process is effectively avoided. The ultraviolet resistance, the strength and the hydrolysis resistance of the film are improved through the synergistic effect of all the components in the formula, so that the application requirements of PET products in the fields of packaging, electronics, photovoltaics, automobiles, medical instruments and the like are met.

The application discloses a kind of generation (4S) 4-hydroxyisophorone monooxygenase mutant and preparation method thereof, the monooxygenase is cytochrome P450 BM3 Monooxygenase from bacillus megaterium. The cytochrome P450 BM3 Monooxygenase mutant has high stereoselectivity and high reaction activity to catalyze isophorone hydroxylation, mutant R47L / Y51F / F81W / A191T / N239H / I259V / A276T / A328I / A330S / L353I / I401L / L437LL catalyzes isophorone to obtain (4S) 4-hydroxyisophorone diastereomeric excess rate can reach 95%, yield can reach 96%; the cytochrome P450 BM3 Monooxygenase mutant catalyzes isophorone synthesis (4S) 4-hydroxyisophorone process is simple, environmental protection, and industrialization prospect is good.

The invention discloses a method for improving the processing quality of tobacco leaves by using a facultative anaerobic bacterium compound preparation, which comprises the following steps: preparing seed solutions of bacillus altitudinis ZY02-1, bacillus subtilis SMXP-58 and klebsiella variicola HNYJ-3, preparing a tobacco extracting solution, compounding the seed solutions according to the ratio of 2: 1: 2, centrifuging, resuspending and culturing by using the tobacco extracting solution to obtain a compound bacterial agent, and preparing the compound bacterial agent by using the facultative anaerobic bacterium compound preparation. And finally, spraying the complex microbial inoculant according to the inoculum size of 2-5% until the primarily-cured tobacco leaves are dampened until the moisture content is 14-15%, sealing, stacking and fermenting at 20-30 DEG C for 3-5 days, and then re-drying. By means of the method, decomposition of polyphenol and terpene aroma precursors of the tobacco leaves can be accelerated, the content of aroma substances such as megastigmatrienone is remarkably increased, aroma components such as oxidized isophorone are added, the aroma quality and the aroma amount of cigarettes are improved, offensive odor is reduced, and the aftertaste and smoking comfort are improved.

The present application relates to the technical field of IPDI synthesis, and particularly discloses a method for removing residual n-butanol in tail gas in a process for synthesizing isophorone diaminon-butyl carbamate by using a urea method, wherein the tail gas is obtained from: ammonia-containing tail gas obtained in the process for synthesizing isophorone diaminon-butyl carbamate by using the urea method, and tail gas obtained after an ammonium carbamate removal process; and the method for removing the residual n-butanol in the tail gas comprises the following steps: cooling the tail gas to 15-25 DEG C, and then adsorbing the n-butanol in the tail gas by using an adsorbent; and the adsorbent comprises molecular sieves, activated carbon and nano-polymer adsorption resin. By using the method, the content of n-butanol in the tail gas of isophorone diaminon-butyl carbamate is far lower than the industrial emission standard, no secondary pollution is caused, and the system safety is high.

The invention belongs to the field of molecular fluorescent probes, and particularly discloses a near-infrared fluorescent probe of quinone oxidoreductase 1 (NAD (P) H: quinone oxidoreductase 1, NQO1) with an in-situ covalent anchoring function and a preparation method of the near-infrared fluorescent probe. According to the probe, dicyanoisophorone is used as a core fluorophore, an aldehyde group is used as an NQO1 covalent anchoring group, and 3-methyl-3-(2, 4, 5-trimethyl-3, 6-dioxocyclohex-1, 4-diene-1-yl) butyrate is used as a recognition group of NQO1. The invention also provides an application of the near-infrared fluorescent probe with the in-situ covalent anchoring NQO1. Compared with other probes, the probe is simple in synthesis method and suitable for industrial production, and has high sensitivity, high selectivity and good biocompatibility in NQO1 detection. The fluorescent probe disclosed by the invention can realize covalent labeling and in-situ imaging functions after target NQO1 recognition response. Long-time in-situ tracking and imaging can be carried out on tumor cells and tissues by monitoring the NQO1 level, and a simple and effective method is provided for accurate detection of tumor disease related protease.

The invention discloses a polyurethane antifouling paint and a preparation method thereof, and belongs to the field of paints.The preparation method comprises the steps that cardanol and formaldehyde are subjected to polycondensation to form a cardanol aldehyde prepolymer, then the cardanol aldehyde prepolymer, polyether polyol and isophorone are mixed and react, and cardanol modified polyurethane is obtained; and mixing a volatile solvent, a flatting agent, nano silicon dioxide, perfluoropolyether oil and cardanol modified polyurethane to obtain the polyurethane antifouling paint. The cardanol structure is introduced, so that the film-forming property of the coating can be improved, active sites can be provided for subsequent polyurethane modification, the cohesion of the coating is enhanced to a certain extent, and the coating is more wear-resistant by matching with the nano silicon dioxide; the cardanol modified polyurethane combines the advantages of cardanol, polyether polyol and isophorone diisocyanate, and perfluoropolyether oil has extremely low surface energy, and can effectively inhibit the adhesion of marine organisms and play an antifouling role under the combined action of the perfluoropolyether oil and other components.

Conventionally, CO2 absorbents such as isophorone diamine are used, and the CO2 absorption process at low temperatures and the CO2 extraction process at high temperatures are carried out in separate tanks. However, once the CO2 absorbent such as isophorone diamine absorbs CO2 and solidifies (changes into carbamic acid), it becomes difficult to move it to the other tank, which presents a problem. [Solution] Even when CO2 absorbents such as isophorone diamine absorb CO2 and solidify (change into carbamic acid), the system does not require movement. By creating a (medium to high) vacuum inside the tank, the amount of air inside the tank is reduced, and the concentration of CO2 extracted from the carbamic acid increases accordingly.

The invention discloses a tissue slice in-situ hybridization pretreatment liquid and a use method, the tissue slice in-situ hybridization pretreatment liquid is characterized in that the tissue slice in-situ hybridization pretreatment liquid is composed of a compound component A solution and a surfactant component B solution, the compound component A solution and the surfactant component B solution have a proper octanol / water partition coefficient and can effectively remove endogenous fluorescent substances, and a solvent is a hydrophilic solvent; wherein the component A is one or a combination of more of bis (hexamethylene) triamine, dodecyl pyridine chloride, cyclohexylamine, isophorone diamine, N-benzylamino ethanol, N, N, N, N-tetramethyl-1, 6-hexamethylenediamine and 1, 3-bis (aminomethyl) cyclohexane; and the component B is one or a combination of more of triton X-100, tween-20, lauryl sodium sulfate and sodium dodecyl benzene sulfonate. The method is used when the FFPE slice is subjected to fluorescence in-situ hybridization or immunofluorescence experiments, autofluorescence can be reduced in a rapid removal mode, the pretreatment effect is remarkably improved, and the hybridization efficiency of samples difficult to hybridize is improved.

The present application relates to a kind of bean-flavored fragrance base module, the bean-flavored fragrance base module includes vanilla bean tincture, dihydrocoumarin, fermented tobacco extract, megastigmatrienone and isophorone oxide.The fragrance base module provided by the present application has typical bean-flavored fragrance and roasted tobacco smoke, and the feeling of fine and soft smoke is better, the aroma quality and smoke comfort are improved, so that the smoke is more round, while reducing the burr feeling of smoke.

The present invention relates to an isophorone dimer prepared from biomass, a method for preparing same, and an alternative aviation fuel comprising same. Specifically, the present invention relates to: an isophorone dimer prepared by forming isophorone using carboxylic acid obtained from biomass and subjecting the isophorone to dimerization, hydrogenation, and deoxygenation; a method for preparing same; and an alternative aviation fuel comprising same.

The invention discloses a waterproof anti-slip coating for bridges and a production process thereof, and relates to the technical field of protective paints.The coating is composed of a component A and a component B. The component A is a prepolymer generated through reaction of phosphorus-containing modified polycarbonate diol and hexamethylene diisocyanate and has good hydrophobicity, the compactness of a coating is improved, and the anti-slip effect is good; the component B is composed of isophorone diamine, a modified chain extender and a surface modified silicon carbide filler, a silicon dioxide coating layer is formed on the surface of silicon carbide through hydrolytic condensation of tetraethyl orthosilicate, further coupling modification is performed through epoxy-fluorine-containing silane, a reactive epoxy group and a hydrophobic fluorine-containing group are introduced, the skid resistance and the medium infiltration resistance of the filler are improved, and the wear resistance of the filler is improved. Active groups in the modified chain extender promote the formation of a cross-linked structure and improve the mechanical property and bonding strength of the coating, so that the coating has high strength, water resistance, corrosion resistance and excellent skid resistance.

The application discloses a preparation method of a catalyst for catalyzing directional polycondensation of acetone to produce mesitylene and isophorone, and belongs to the field of catalysis technology. The catalyst is prepared by using a co-precipitation method to prepare magnesium-aluminum-zinc composite oxides, and simultaneously introducing active components and metal modified additive components in the co-precipitation process, and then drying and calcining to obtain a finished catalyst. The application realizes efficient synergistic effect of the active metal components and the additive metal components on the composite basic oxides and adjustment of the distribution of acid and base sites of the catalyst, so that the effect of co-producing isophorone and mesitylene through directional polycondensation of acetone is achieved. The catalyst prepared by the method is suitable for catalyzing polycondensation of acetone to co-produce isophorone and mesitylene, and has the advantages of simple preparation process, high catalytic activity and good stability.

The invention relates to a plant-source attractant for peach tree pests and application of the plant-source attractant. The plant-source attractant is selected from any one or more of beta-phellandrene, 3-methylene-6-(tert-amyl) cyclohexane-1-ene, 1-isopropyl-4-methylene cyclohexane, 4-methylene cyclohexane-1-ethyl formate, 4-oxoisophorone and 3-carene, and the plant-source attractant is selected from any one or more of beta-phellandrene, 3-methylene-6-(tert-amyl) cyclohexane-1-ene, 1-isopropyl-4-methylene cyclohexane, 4-methylene cyclohexane-1-ethyl formate, 4-oxoisophorone and 3-carene. The compound can be used for preparing a preparation for attracting peach tree pests outdoors. Compared with the prior art, the peach tree pest plant source attractant can trap and kill female adults in external environments such as a field, and the prevention and control effect is greatly improved.

The invention relates to an amine curing agent, thermal viscosity-reducing glue, a thermal viscosity-reducing carrier membrane and a preparation method thereof, the amine curing agent comprises cardanol, isophorone diamine and a furfural solution, the cardanol is melted at the temperature of 50-60 DEG C, then the isophorone diamine is added for reaction at the temperature of 60-70 DEG C, then the furfural solution is added for condensation reaction at the temperature of 75-90 DEG C, and the thermal viscosity-reducing carrier membrane is obtained. And finally, cooling to room temperature to obtain the amine curing agent. The thermal visbreaking carrier film prepared from the amine curing agent and the thermal visbreaking glue not only can provide stable and reliable adhesive force at normal temperature and ensure the stability of process operation, but also can be smoothly peeled off after high-temperature working sections such as cover film pressing and solder resist ink baking, the surface of a PI film is kept clean, and the service life of the PI film is prolonged. The manufacturing efficiency and reliability of the flexible circuit board are obviously improved; the thermal visbreaking carrier film has the advantages of high-temperature visbreaking, no adhesive residue, good dimensional stability and the like.

According to the ultrathin PET functional master batch and the preparation method thereof disclosed by the invention, UV absorbing groups such as isophorone diester and 2, 4-dihydroxybenzoic acid are introduced, so that the absorption capacity of a molecular chain to ultraviolet rays is enhanced, and meanwhile, the aging resistance of a film is improved. Besides, by utilizing the Beta molecular sieve, the zinc metaborate and other auxiliaries, the uniform distribution of the light stabilizer and the ultraviolet light absorber in the molecular structure is improved, and the non-uniform UV absorption performance of the film caused by molecular arrangement change in the stretching process is effectively avoided. Due to the synergistic effect of the components in the formula, the ultraviolet resistance, strength and thermal stability of the film are improved, the performance uniformity of the film is ensured, and an innovative material solution is provided for preparation of the high-performance ultrathin PET film. According to the preparation method disclosed by the invention, the properties, such as mechanical strength, thermal stability and gas barrier property, of the PET material can be remarkably improved, so that the application requirements of ultrathin products in the fields of packaging, electronics, buildings and the like can be met.

The application discloses a self-emulsifying polyether amine curing agent and a preparation method and application thereof, and belongs to the field of high polymer materials. According to the application, components A-F are composed of polypropylene glycol or polytetrahydrofuran glycol with a molecular weight range of 800-2000, component B is selected from at least one of isophorone diamine, dicyclohexyl methane diamine or xylylenediamine, component C is at least one of epoxy derivatives containing long-chain alkyl and aromatic ring structures, component D is polyethylene glycol monomethyl ether amine with a polymerization degree range of 2-5 or an amine compound containing a sulfonic acid group, component E is a tertiary amine compound, and component F is at least one of a hindered phenol antioxidant and a phosphite antioxidant. The curing agent is reliably enhanced in reaction activity at normal temperature, the nucleophilic activity of terminal amino groups is significantly improved while excellent water dispersibility is maintained, and thus the curing rate at normal temperature is greatly accelerated.

The application discloses a system for producing isophorone and mesityl oxide by a gas phase condensation method, characterized in that a ketone vaporization tower is provided with a ketone inlet, the ketone vaporization tower is sequentially connected with a first ketone gas phase condensation reactor and a second ketone gas phase condensation reactor, the second ketone gas phase condensation reactor is connected with a ketone recovery tower through a ketone heat exchanger, the ketone recovery tower is connected with the ketone vaporization tower, the ketone recovery tower is connected with a decanter through a ketone recovery tower bottom cooler, a water phase outlet of the decanter is connected with a waste water tower, an oil phase outlet of the decanter is connected with a light component removal tower, the light component removal tower is connected with a mesityl oxide refining tower, the light component removal tower is connected with a mesitylene refining tower, a top of the mesitylene refining tower is provided with a mesitylene product outlet, the mesitylene tower is connected with an isophorone refining tower, and the isophorone refining tower is provided with an isophorone product outlet.

This invention discloses a fluorescent probe that simultaneously distinguishes between hydrogen peroxide and glutathione. The chemical structure of the probe is as follows: This fluorescent probe ingeniously combines a 1,8-naphthalenedimide fluorophore and an isophorone fluorophore, achieving for the first time a dual-channel simultaneous detection of hydrogen peroxide and glutathione. When reacting with glutathione, it emits 500 nm green fluorescence at an excitation wavelength of 380 nm; when reacting with hydrogen peroxide, it emits 680 nm near-infrared fluorescence at an excitation wavelength of 560 nm. The two emissions differ by 180 nm, and the spectra do not interfere with each other, allowing for the selective simultaneous detection of hydrogen peroxide and glutathione. This probe exhibits excellent selectivity for detecting hydrogen peroxide and glutathione, high fluorescence quantum yield, and a large Stokes shift, showing great promise for applications in analytical chemistry, life sciences, and biomedicine.

The present application relates to a kind of preparation methods of 3-aminomethyl-3,5,5-trimethylcyclohexylamine, the preparation method described includes: first isophorone nitrile and liquid ammonia occur imination reaction in the presence of imination catalyst, the obtained imination reaction liquid is mixed with methanol and then enters hydrogenation reactor, and hydrogenation reaction occurs in the presence of hydrogenation catalyst to obtain 3-aminomethyl-3,5,5-trimethylcyclohexylamine.Using the method provided in the present application, relatively low reaction pressure can be used to obtain a reaction solution with a lower content of difficult-to-separate impurities, which reduces the difficulty of separation and reduces the difficulty of separation.In addition, the mixing heat of methanol and liquid ammonia can be used for hydrogenation preheating, reducing energy waste, in line with the current low-carbon development trend, further reducing the operating cost of the device.

The invention belongs to the technical field of ion detection, and particularly relates to a preparation method of a long-wavelength emission fluorescent probe for rapidly detecting bisulfite, which comprises the following steps: dissolving isophorone and malononitrile in ethanol to obtain a product 1; 3-bromoquinoline and 5-formyl-2-thiopheneboronic acid are dissolved in a mixed solvent of dry methanol and dry toluene, and a product 2 is obtained; dissolving the product 2 in dichloromethane, and adding methyl trifluoromethanesulfonate to obtain a product 3; and dissolving the product 1 and the product 3 in ethanol, and adding piperidine to obtain the required fluorescent probe LSQ. Dissolving the obtained fluorescent probe LSQ in DMSO to prepare mother liquor A; a target product is obtained. The fluorescent probe provided by the invention has the advantages of high-selectivity rapid response to bisulfite, long-wavelength emission, no organic solvent detection, simple synthesis route and simple and convenient detection equipment and method, and can be used for qualitative and quantitative detection of bisulfite in food and biological systems.

This invention belongs to the field of fluorescent probes and discloses a probe that can simultaneously respond to ClO ‑ Tetraphenylethylene-thiophene hybrid fluorescent probes with varying viscosity and their preparation methods and applications. The probes utilize a tetraphenylethylene-aromatic ring hybrid structure as the chromophore, with dinitrile isophorone and diaminomaleitrile as ClO₂ groups, respectively. ‑ Based on the recognition group, fluorescent probe molecules were designed and synthesized, with the following general structural formula: This type of fluorescent probe is effective against ClO₂. ‑ It exhibits rapid response, high specificity, and high sensitivity, with a strong responsiveness to viscosity, and can simultaneously detect ClO. ‑ Changes in viscosity levels and the environment provide new insights and potential detection tools for the early diagnosis of atherosclerosis.

The invention relates to the field of water-soluble resin, and discloses water-based alkyd resin as well as a preparation method and application thereof. The water-soluble resin disclosed by the invention is prepared from 20 to 24 parts of erythritol, 6 to 8 parts of isosorbide, 10 to 15 parts of neodecanoic acid, 15 to 20 parts of succinic acid, 15 to 20 parts of sebacic acid, 2 to 3 parts of citric acid, 5 to 7 parts of isophorone diamine, 9 to 10 parts of linolenic acid propylene glycol ester and a catalyst. Under the condition that an emulsifier, a cosolvent and a large amount of neutralizer are not added, the emulsion can rapidly form a uniform milky-white dispersion with the same amount as water, and the emulsion is free of flocculation, layering and precipitation after standing at 25 DEG C for 30 days. Technological parameters are simple and controllable, the system is low in foam and easy to defoam, and large-scale amplification is facilitated; meanwhile, the dosage of a cosolvent and a neutralizer is reduced, VOC and odor are reduced, and the water-based environment-friendly requirement is met.

The invention belongs to the field of fluorescent probes, and discloses a selenium cyano fluorescent probe for high-selectivity recognition of H2S as well as a preparation method and application of the selenium cyano fluorescent probe. The probe adopts dicyanoisophorone as a fluorophore skeleton and-SeCN group as a novel recognition site, and the structural formula is shown in the specification. The dicyanoisophorone has relatively large Stokes shift during release, so that the interference of autofluorescence on signal detection is reduced, and the dicyanoisophorone is connected with a-SeCN group through a condensation reaction. On the basis of a double nucleophilic substitution strategy, H2S induces conversion of-SeCN into-Se-S, and near-infrared fluorophores are released through intramolecular self-cyclization. Researches show that the probe has high sensitivity, selectivity, good water solubility and thiol consumption resistance, the detection limit is as low as 20 nM, and the probe has good ratio signal and brain targeting ability.

The invention provides an isophorone dye derivative LYC-CF3 as well as a synthesis method and application thereof. The specific name of the isophorone dye derivative is (E)-4-(2-(3-(dicyanomethylene)-5, 5-dimethylcyclohex-1-ene-1-yl) vinyl)-2-(trifluoromethyl) phenyl trifluoromethanesulfonate, and the structural formula of the isophorone dye derivative is shown in the description. The invention also provides a synthesis method of the derivative LYC-CF3, and a fluorescence imaging experiment of the derivative LYC-CF3 on O2 <-> in PC-12 cells. Through verification of a fluorescence spectrum and a laser confocal microscope, the derivative LYC-CF3 has quick response to O2 <-> in an in-vitro test and cells and relatively remarkable fluorescence imaging capability. Therefore, LYC-CF3 can be used as a fluorescent probe for in-vitro detection of O2 <-> and detection of O2 <-> in cells.

The application belongs to the technical field of tumor cell optical imaging, and particularly relates to a preparation method and application of a near-infrared fluorescent probe for specifically detecting SHP2. H The near-infrared fluorescent probe has a structure as shown in I, wherein R is a dicyanoisophorone fluorescent group or a dicyano methylene-4 The probe has a novel structure, strong binding affinity to SHP2 protein, good fluorescence characteristics, fast reaction time, strong specificity, and can specifically recognize SHP2 protein. The near-infrared fluorescent probe can be used for labeling tumor cells with high expression of SHP2 protein. The probe raw material is cheap and easy to obtain, the reaction steps are simple, and the post-treatment process is simple.

The present invention relates to a continuous process for the liquid-phase synthesis of isophorone by alkaline self-condensation of acetone, comprising the following successive steps:a) continuous injection through a tubular reactor (R) of a stream of an aqueous alkali metal hydroxide solution and of a stream of an organic solution comprising acetone and by-products recycled via step g), followed byb) condensation reaction of acetone within the tubular reactor (R), followed byc) distillation of the reaction mixture obtained from the tubular reactor (R), followed byd) separation of the concentrated crude reaction mixture obtained from the distillation of step c) resulting in an alkaline aqueous phase and an organic phase containing isophorone, followed bye) distillation of the organic phase containing isophorone recovered in the preceding step, followed byf) distillation of the polycondensation by-products from the bottom of the column (D2) obtained from the preceding distillation, followed byg) recycling of the stream obtained from the top of the distillation column (D3) of the preceding step comprising xylitones and / or isoxylitones to the tubular reactor (R).