307 results about "Anaerobic bacterium" patented technology

A device and method for enhancing nitrogen and phosphorus removal based on a multistage AO partial denitrification coupled with Anammox process in combination with a sludge hydrolytic acidification mixture belong to the technical field of active sludge method wastewater treatment. A system includes a water tank, a water pump, a biochemical reaction zone, a hydrolytic acidification tank and other devices. A multistage AO step-feed pipeline is used to inject raw water into the reaction zone in a segmented manner, guaranteeing efficient utilization of organic matter in the raw water; biofilm carriers are added into an anaerobic zone and anoxic zones to enrich anammox bacteria, and nitrite nitrogen produced by partial denitrification provides a substrate for the anammox bacteria to realize autotrophic nitrogen removal; a nitrification and phosphorus accumulating bacteria aerobic phosphorus uptake are performed in aerobic zones; and part of excess sludge in a secondary sedimentation tank enters the hydrolytic acidification tank to convert macromolecular organic matter into low molecular weight organic matter, a hydrolytic acidification mixture and the excess sludge in the secondary sedimentation tank synchronously flow back to the anaerobic zone, and as a high-quality carbon source, the low molecular weight organic matter can promote partial denitrification. The system provides a novel method for efficient and energy-saving treatment of municipal wastewater.

The invention relates to the technical field of environmental biology, in particular to medium-temperature and high-temperature anaerobic bacteria for increasing methane yield and relieving ammonia inhibition and application of the medium-temperature and high-temperature anaerobic bacteria. The invention provides the Anaerobic zm02 with the strain number of CGMCC No.46069. The Anaerobic zm02 can obviously improve the organic matter degradation efficiency and the methane yield of an anaerobic digestion system, can effectively relieve ammonia inhibition, can be applied to fermentation treatment of wastes, and has a very good application prospect.

The invention belongs to the technical field of wastewater treatment and energy recovery, and provides a regulation and control method for promoting anaerobic acid production by taking soybean molasses as a substrate through cooperation of ferroferric oxide and bamboo charcoal. Soybean molasses is used as a substrate carbon source, anaerobic activated sludge is inoculated in a medium-temperature SBR reactor, the volume ratio of reactor inflow sewage to sludge is 2: 1, ferroferric oxide and bamboo charcoal composite conductive particles which are subjected to ball milling treatment and have the concentration of 1 g / L are added to promote an anaerobic fermentation system to produce acid, and then anaerobic fermentation is carried out. The mass ratio of ferroferric oxide to bamboo charcoal in the ferroferric oxide and bamboo charcoal composite conductive particles is (1: 2)-(2: 1); monitoring the acid yield, the pH value change and the product type; and the variety and concentration of the acid-producing substance are analyzed, and the interaction of all the components in the reaction process is evaluated. The acid production rate and the total acid production amount in the anaerobic acid production process are obviously improved. And the activity of anaerobic flora is promoted. The variety distribution of acid-producing substances is obviously optimized, and the stability of the acidification process is improved.

The invention discloses a compound micro-ecological preparation for aquatic products as well as a preparation method and application thereof, and belongs to the technical field of micro-ecological preparations. The microecological preparation disclosed by the invention comprises clostridium butyricum, bacillus licheniformis, enterococcus faecalis and lactobacillus plantarum. The microecological preparation is combined with concentrated cottonseed protein for use, can replace fish meal, is added into feed, can remarkably improve the immunocompetence, oxidation resistance and digestive enzyme activity of animals, and can improve the relative abundance of beneficial bacteria in intestinal tracts, inhibit the growth of pathogenic bacteria and regulate the microecological balance of the intestinal tracts after entering the intestinal tracts of the animals. According to the invention, aerobic bacteria and anaerobic bacteria are cooperatively used, oxygen in the surrounding environment is greatly consumed by bacillus licheniformis and enterococcus faecalis, the proliferation of beneficial bacteria such as clostridium butyricum and lactobacillus plantarum is promoted, and the probiotic effect is fully played.

The invention discloses a salt-tolerant denitrifying bacterium and application of the salt-tolerant denitrifying bacterium, the salt-tolerant denitrifying bacterium is specifically Stutzerimonas degrandans and is named as Stutzerimonas degrandans BP, the preservation number of the salt-tolerant denitrifying bacterium is GDMCC No.64297, and the salt-tolerant denitrifying bacterium is preserved in the Guangdong Microbial Culture Collection Center on January 19, 2024. When the Stutzerimonas degrandans BP provided by the invention is used for biological nitrogen removal of high-salinity wastewater, the Stutzerimonas degrandans BP has relatively strong salt-tolerant denitrification capability, NO3 <->-N in a high-salinity water body can be effectively removed, no by-products such as NO2 <->-N and NH4 < + >-N are accumulated, and secondary pollution cannot be caused. Experimental results show that the Stutzerimonas degrandans BP provided by the invention is a facultative anaerobic bacterium, and is suitable for biological nitrogen removal in an anaerobic or anoxic environment. Under an anaerobic condition, high-efficiency salt-tolerant denitrification can be realized by virtue of relatively small biomass (OD600 = 0.15-0.25), so that the yield of sludge can be reduced while the operation cost of the process is reduced.

The invention provides a high-efficiency low-pollution cow dung returning treatment method, which comprises the following steps: adding corn straw and charcoal into cow dung, preparing a fermentation base material, inoculating a compound fermentation inoculant, and carrying out composting fermentation to prepare a cow dung organic fertilizer. The composite microbial inoculant adopts an inner and outer double-film coating structure, aerobic bacteria are embedded in the middle, are released in the early stage of composting, quickly enter a high-temperature stage and decompose and metabolize organic matters, anaerobic bacteria are embedded in the innermost layer, and an inner film coating material adopts composite modified waterborne polyurethane, so that the composite microbial inoculant has a good anti-bacterial effect. Azobenzene and waxberry tannin combined modification endows the membrane structure with the characteristics of low-oxygen cracking, normal-oxygen stability and no influence of enzyme, anaerobic bacteria are released under the low-oxygen condition in the high-temperature last stage and the decomposition period, decomposition of residual cellulose can be promoted, carbon and nitrogen loss caused in anaerobic fermentation is prevented, the speed of converting organic matter into humus is increased, and the yield of the organic matter is increased. Meanwhile, the inner film packing material reacts with the organic acid and the ethanol, so that the quality of the cow dung organic fertilizer is further ensured.

The invention relates to the field of sewage treatment, and provides double-layer anaerobic bacteria-algal-bacterial symbiotic immobilized particles as well as a preparation method and application thereof. The double-layer anaerobic bacteria-algal-bacterial symbiotic immobilized particle sequentially comprises a PAN (polyacrylonitrile) fiber particle, an anaerobic bacteria layer, a polymer film, an algal-bacterial symbiotic layer and a calcium alginate gel layer from inside to outside. The preparation method comprises the following steps: modifying PAN fiber particles to enable the PAN fiber particles to carry positive charges, and putting the modified PAN fiber particles into an anaerobic bacteria solution for dipping adsorption; coating a PVDF (Polyvinylidene Fluoride) solution to form a polymer film, so as to obtain PAN (Polyacrylonitrile) fiber particles for immobilizing anaerobic bacteria; soaking in a mixed solution of an algal-bacterial symbiotic suspension and sodium alginate, taking out, putting into a calcium chloride solution, and cross-linking and hardening to form gel. Anaerobic microorganisms and aerobic microorganisms in the immobilized particles are subjected to regional division and immobilization, and the inner-layer anaerobic microorganisms and the outer-layer aerobic microorganisms play a greater role in different regions of a carrier by virtue of a physiological synergistic effect among the microorganisms, so that carbon, nitrogen and phosphorus in sewage are deeply removed.

The invention discloses a high-sealing ingredient stirring tank, and relates to the technical field of sewage treatment.The high-sealing ingredient stirring tank comprises an outer bin body and an inner bin body, the inner bin body comprises a first cavity and a second cavity, the first cavity is arranged around the second cavity, the first cavity is rotationally connected with the outer bin body, and a plurality of refrigeration pieces are installed on the outer wall of the second cavity; a feeding pipe is arranged in the second cavity, the end, away from the bottom of the second cavity, of the feeding pipe is rotationally connected with a feeding head, and the feeding head is connected with a feeding pipeline; the refrigeration piece comprises a low-temperature face and a high-temperature face, the low-temperature face is in heat conduction connection with the outer wall of the second cavity, a heat conduction column is arranged on the high-temperature face, and a part of the heat conduction column is located between the outer bin body and the first cavity. Therefore, through combined use of the outer bin body and the inner bin body, the anaerobic flora is pre-activated, and the anaerobic flora can proliferate in advance after being activated, so that the feeding amount of anaerobic bacteria is reduced, and the working efficiency of the anaerobic tank can be improved.

The invention belongs to the technical field of biomass anaerobic treatment, and particularly discloses a method for preparing methane through biomass anaerobic digestion based on oxygen nano bubble water. Biomass, activated sludge, oxygen nano bubble water and water are mixed, anaerobic digestion treatment is carried out, and methane is obtained. By introducing high-stability oxygen nano bubble water, a suitable micro-aerobic environment is provided for facultative anaerobic bacteria, so that the metabolic activity of the facultative anaerobic bacteria is activated, the decomposition and conversion of complex organic matters are accelerated, and the efficiency of a hydrolytic acidification stage is remarkably improved. Compared with a traditional chemical or physical pretreatment mode, the method has the advantages that interference on anaerobic flora is avoided, the degradability of a substrate is effectively enhanced, and more sufficient organic acid is provided for a subsequent methane production stage, so that the yield of methane is increased.

The invention relates to a method and system for in-situ treatment of fluorine-containing mine water in cooperation with a coal mine underground reservoir. The method comprises the following steps: S1, screening coal gangue of which the coal content is 5-20% and the particle size is less than or equal to 5mm; s2, fluorine-containing mine water or sediment collected near the site to be treated is used as a sample, the sample is subjected to screening, enrichment and domestication of mixed anaerobic flora, and efficient defluorination mixed bacteria are obtained; s3, the bacterial liquid of the efficient defluorination mixed bacteria and the coal gangue are subjected to mixed culture, and microbial biofilm culturing coal gangue is obtained; and S4, adding the microbial biofilm formation coal gangue into a material adding mechanism, and adding the microbial biofilm formation coal gangue into mine water of a coal mine underground reservoir through the material adding mechanism for defluorination treatment. According to the invention, in-situ defluorination can be carried out on the mine water of the coal mine underground reservoir, and the purification treatment effect of the coal mine underground reservoir on the mine water is greatly improved.

The invention discloses a high-sensitivity oxygen-resistant anode MFC (Microbial Fuel Cell) sensor for biotoxicity detection and a preparation method thereof. The sensor comprises a positive electrode, a negative electrode, a positive electrode chamber, a negative electrode chamber, a diaphragm, a current loop and a data acquisition system, a double-layer biological membrane is formed on the positive electrode through aerobic domestication, the outer layer is an aerobic bacteria layer, and the inner layer is an anaerobic electroactive bacteria layer. The aerobic bacteria on the outer layer consume dissolved oxygen and provide a stable environment for the anaerobic bacteria on the inner layer, so that the oxygen resistance and the operation stability of the sensor are remarkably improved. When the toxicity of a to-be-detected sample is detected, the biotoxicity of the sample is represented through the electrogenesis inhibition rate of the sensor. Experiments show that the response sensitivity of the sensor to toxic substances (such as Cu (II)) is obviously higher than that of a traditional anaerobic MFC sensor, and the sensor shows excellent anti-interference capability under different dissolved oxygen conditions. The method is suitable for rapid detection of biotoxicity of water and environmental samples, and has the characteristics of high sensitivity, strong oxidation interference resistance and good stability.

The invention relates to a specific culture medium for detecting propionibacterium acnes in cosmetics and a detection method thereof, and belongs to the technical field of microbiological detection. Every 1000 mL of the specific culture medium contains 5-25 g of a nitrogen source, 5-10 g of a carbon source, 0.02-16 g of an inhibitor, 0.01-0.02 g of a color indicator, 0.1-13 g of a conditioning agent and the balance of a solvent. The inhibitor comprises Tween 80, a tea tree oil extract, cholate, sodium laurate and hydroxyproline. The specific culture medium provided by the invention is a propionibacterium acnes selective culture medium which is free of antibiotics, high in selectivity, sensitive in color development and low in cost. By optimizing the components of the culture medium and adding the selective inhibitor and the color indicator, the growth of other facultative anaerobic bacteria such as lactic acid bacteria can be effectively inhibited, specific culture and color development detection of propionibacterium acnes are carried out, and the accuracy and reliability of detection are improved.

The utility model discloses a micro-power nitrogen removal tank. The micro-power nitrogen removal tank comprises a first chamber, a second chamber, a fluid discharge channel and a gas supply unit, wherein the first chamber is used for reducing oxygen content; the second chamber is used for removing nitrogen; the first chamber is provided with a water inlet, the upper part of the first chamber is communicated with the upper part of the second chamber, and the lower part of the second chamber is communicated with the lower part of the fluid discharge channel; the gas supply unit is communicated with the lower part of the fluid discharge channel and is used for introducing gas into the fluid discharge channel; during application, aerobic bacteria can be added into the first chamber, and anaerobic bacteria can be added into the second chamber; sinking the micro-power nitrogen removal tank added with the strain into a sewage water body; the micro-power nitrogen removal tank disclosed by the utility model can realize flowing of an internal water body only under the action of airflow, is extremely low in energy consumption, and can assist in realizing removal of total nitrogen under the condition that the ecology of a natural water body is not changed.

The invention relates to the technical field of blood culture bottles, in particular to a composition and application thereof in preparation of a microbial culture medium. Yeast extract powder, soy peptone and casein tryptone are combined into a nitrogen source, and an aerobic and anaerobic culture medium for clinical pathogenic microorganism culture is formed. Through comparison verification, in growth experiments of nine aerobic bacteria and seven anaerobic bacteria, the culture speed and the biomass accumulation of the aerobic and anaerobic culture media added with the composition are integrally superior to those of other components excluded in the prior art and screening. Therefore, the composition can effectively promote the performance of a blood culture product, improve the clinical blood flow infection detection capability, inhibit antibiotic abuse and save the life of a patient.

The invention provides a beef slaughter wastewater resourceful treatment system, and relates to the technical field of sewage treatment.The beef slaughter wastewater resourceful treatment system comprises a treatment box, an opening is formed in the upper end of the treatment box, a flocculation box is fixedly installed at the lower end of the treatment box, an anaerobic bacteria reaction tank is arranged on one side of the treatment box, and a division disc is rotationally installed in the treatment box through a bearing; six treatment cavities are symmetrically formed in the circle center of the interior of the grid dividing disc, an annular screen is fixed to the outer side of the grid dividing disc, and openings in the outer ends of the treatment cavities are wrapped with the annular screen. Primary wastewater and flocculated wastewater with precipitates are isolated and filtered through a first sieve plate, a second sieve plate and an annular screen respectively, in order to avoid accumulation of more impurities difficult to clean on the annular screen, alternate backwashing and impurity treatment of a plurality of treatment cavities are realized through a cleaning assembly, and the impurities are sent out, so that the time spent on manual cleaning is saved, and the cleaning efficiency is improved. And the cleaning time and resources are saved.

The invention relates to the technical field of environmental remediation microorganisms, and discloses acinetobacter B2-4, a culture method thereof and application of the acinetobacter B2-4 in degradation of polycyclic aromatic hydrocarbon. The Acinetobacter sp. B2-4 is preserved in the China General Microbiological Culture Collection Center on March 3, 2025, the preservation address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC No.33689. The Acinetobacter sp. B2-4 has the advantages that the Acinetobacter sp. The Acinetobacter sp. B2-4 disclosed by the invention is an anaerobic bacterium, and can utilize nitrate, iron, sulfate and the like to replace an electron acceptor in an anoxic environment to continuously degrade polycyclic aromatic hydrocarbon. After the strain is cultured for 45 days, the degradation rate of phenanthrene reaches 74.9%, and the degradation rate of pyrene reaches 56.2%.

The invention discloses a lithium battery precious metal recovery method based on microorganism-electrochemical coupling and one-way membrane separation, which comprises the following steps: step 1, pretreatment of a waste lithium battery: performing complete discharge treatment on the waste lithium battery, then disassembling the battery in an oxygen-free environment, separating out a positive electrode material and a negative electrode material, and drying the positive electrode material and the negative electrode material; electrode material black powder containing precious metal is obtained; 2, designing an anaerobic tank, and culturing and domesticating an acid-producing electroactive flora; 3, continuously and stably adding black powder into the anaerobic tank; copper electrodes are added to the two sides of the cylindrical anaerobic tank and the center of the one-way membrane, a power source is connected, the positive electrode is connected with the electrodes on the two sides of the anaerobic tank, and the negative electrode is connected with the membrane center electrode. Compared with the prior art, the method has the advantages that the solid-to-liquid ratio of microbiological leaching can be greatly increased through microbiological-electrochemical coupling and one-way membrane separation, the transfer efficiency of precious metal electrons is accelerated, anaerobic flora is protected, poison to the anaerobic flora is reduced, and the service life of equipment is prolonged.

The utility model provides a pair of full-hemp surface layer menstrual period trousers, which comprises a full-hemp surface layer menstrual period trousers body which is in the shape of underpants formed by cutting and compounding non-woven fabrics and rubber bands. The full-China-hemp surface layer menstrual period pants body sequentially comprises a surface layer, a flow guide layer, an absorber, an anti-leakage bottom film and an elastic surrounding body from the skin contact surface to the bottom, and the surface layer is made of full-China-hemp non-woven fabric. The surface layer is the non-woven fabric made of all China-hemp fibers, the China-hemp fibers belong to natural plant fibers and can destroy the thallus structure of bacteria and prevent anaerobic bacteria from surviving, the China-hemp fibers have moisture absorption and quick drying functions, a dry environment is not beneficial to bacterial reproduction, menstrual blood is quickly pulled to the bottom through the multi-layer tiled structure in the absorber, and therefore the menstrual blood can be quickly absorbed. The China hemp fibers can effectively inhibit bacterium breeding and prevent bacteria from breeding upwards, skin or gynecological diseases caused by contact with skin are avoided, and the use requirement of women for menstrual period trousers is met.

A medium supplement for high-yield culture of anaerobes is disclosed. The medium supplement includes N-acetylhexosamine, L-aspartic acid, L-cysteine and cobalamin. A culture medium including the supplement and a culture method using the culture medium are also disclosed. It is possible to provide an innovative method which is capable of achieving high-concentration culture of anaerobes that are difficult to culture in high yield. The method is cost-effective, and in particular, is capable of culturing large amounts of fastidious aerobes suitable for use in food and pharmaceutical applications.

The invention relates to a composite microbial inoculant and an evaluation method for the antibacterial effect thereof. The method optimizes the in-vitro culture mode of the microbial agent compounded by aerobic bacteria and anaerobic bacteria by simulating the intestinal environment of probiotics in an animal body, and adopts a facultative anaerobic culture method for the microbial compound agent, namely, the outer layer of a sealing film of a triangular flask is wrapped with four layers of preservative films, and the neck of the triangular flask is tightened by a rubber band. The facultative anaerobic environment of aerobic bacillus and facultative anaerobic enterococcus faecalis is considered in the microbial composite inoculant, in the subsequent oxford cup bacteriostasis evaluation experiment, the experiment result with the accurate bacteriostasis effect and good parallelism can be obtained, and the action effect of bacillus and enterococcus faecalis composite bacteria can be efficiently and scientifically evaluated.

The utility model discloses a sewage treatment device which comprises a homogenizing tank, one side of the homogenizing tank is connected with a monitoring room through a first conveying pipe, the homogenizing tank is connected with a biological fluidization tank through a second conveying pipe, one side of an anaerobic digestion tank is connected with an ozone tank through a pipeline, and one side of the ozone tank is connected with a biological aerated filter through a pipeline. A plurality of aerators are arranged in the biological aerated filter, stirring blades and a motor are arranged in the homogenizing tank, and different kinds of sewage from the septic tank effluent collecting tank, the domestic sewage inlet and the production wastewater inlet can be fully mixed through stirring, so that the quality and the quantity of the sewage entering a subsequent treatment unit are more uniform; according to the sewage treatment system disclosed by the invention, by adopting a mode of synergism of multiple treatment processes, the anaerobic digester can decompose macromolecular organic matters into micromolecules by utilizing anaerobic bacteria, and multiple processes are combined, so that various pollutants such as organic matters, heavy metals and toxic and harmful substances in sewage are more thoroughly removed, and strict environment-friendly emission standards can be better met.

The invention provides an aquatic product fermented feed containing probiotics. The fermented feed is prepared from the following raw materials: fish meal, soybean meal, peanut meal, flour, bone meal, fish soluble, soybean oil, phospholipid and the probiotics. According to the invention, the breathing valve fermentation bag is used, so that anaerobic bacteria and probiotics which play a role are cultivated and fermented under an anaerobic or semi-anaerobic condition. Meanwhile, the breeding and growth of aerobic harmful bacteria can be inhibited through anaerobic culture, and the growth of beneficial bacteria such as bacillus, lactic acid bacteria and clostridium butyricum is facilitated; the compounded probiotic group is screened, acid can be stably produced within a short time, the acid production content is high, and after a period of culture, the biological growth curve of the screened probiotic group can reach a balanced state within about 2 days; the compounded probiotic group can generate butyric acid, lactic acid and other substances through metabolism, so that the PH of the digestive tract of the fish body is reduced, the reproduction of beneficial bacteria is promoted, and the growth of harmful bacteria is inhibited.

The present invention discloses a method for improving black soil by applying a manure fertilizer in a furrow combined with returning straw to the field. The method comprises the following steps: returning straw to the field: after harvesting the previous crop, crushing the previous crop straw and returning it to the field; preparing a composite inoculant: inoculating psychrophilic bacteria, cellulolytic bacteria, and anaerobic bacteria into LB culture medium to form a bacterial solution, diluting the bacterial solution 20-100 times, mixing them in a volume ratio of (1-2):(1-2):(1-2), and adding auxiliary materials to prepare a composite inoculant; applying the composite inoculant into the 0-15 cm soil layer through shallow furrows along the planting rows at a dosage of 100-150 kg / mu; and sowing seedling strips in alternate years. The method of the present invention can significantly increase the organic matter content of black soil, improve soil aggregate structure, reduce the salinity of the plow layer, and achieve rapid soil fertilization and stable crop yield and efficiency.

The invention discloses a method for improving the processing quality of tobacco leaves by using a facultative anaerobic bacterium compound preparation, which comprises the following steps: preparing seed solutions of bacillus altitudinis ZY02-1, bacillus subtilis SMXP-58 and klebsiella variicola HNYJ-3, preparing a tobacco extracting solution, compounding the seed solutions according to the ratio of 2: 1: 2, centrifuging, resuspending and culturing by using the tobacco extracting solution to obtain a compound bacterial agent, and preparing the compound bacterial agent by using the facultative anaerobic bacterium compound preparation. And finally, spraying the complex microbial inoculant according to the inoculum size of 2-5% until the primarily-cured tobacco leaves are dampened until the moisture content is 14-15%, sealing, stacking and fermenting at 20-30 DEG C for 3-5 days, and then re-drying. By means of the method, decomposition of polyphenol and terpene aroma precursors of the tobacco leaves can be accelerated, the content of aroma substances such as megastigmatrienone is remarkably increased, aroma components such as oxidized isophorone are added, the aroma quality and the aroma amount of cigarettes are improved, offensive odor is reduced, and the aftertaste and smoking comfort are improved.

The invention discloses a method for treating PBAT wastewater by zero-energy-consumption enhanced anaerobic bacteria, which comprises the following steps: (1) uniformly mixing Fe3O4 and GAC according to a certain mass ratio, so that Fe3O4 powder is uniformly attached to the surface of the GAC to prepare a Fe3O4-coated GAC complexing agent, and adding the Fe3O4-coated GAC complexing agent into an anaerobic reactor filled with PBAT wastewater and anaerobic sludge; and (2) forming a magnetic field environment on the outer side of the anaerobic reactor, and coupling the Fe3O4-coated GAC complexing agent with an exogenous magnetic field to form an endogenous current to strengthen anaerobic bacteria in the anaerobic sludge to treat the PBAT wastewater until COD is not degraded any more. According to the zero-energy-consumption method for strengthening anaerobic bacteria treatment of the PBAT wastewater, no chemical agent needs to be added, the operation cost is low, secondary pollution is avoided, generated fuel gas such as methane can be recycled, secondary economic benefits can be generated, the method has the advantages of being efficient, environmentally friendly and economical, and a new solution is provided for treatment of the PBAT wastewater.

The invention discloses an anaerobic bacterial agent and a method for improving the anaerobic methane production rate of medium-temperature and medium-temperature cellulose. The compound anaerobic microorganism liquid bacterial agent is prepared from clostridium termite, thermophilic anaerobe of ethanol, clostridium swardii and methanosarcina barkeri, and the compound anaerobic microorganism liquid bacterial agent is prepared from the following raw materials: clostridium termite, thermophilic anaerobe of ethanol, clostridium swardii and methanosarcina barkeri, and the compound anaerobic microorganism liquid bacterial agent is prepared from the following raw materials: the compound anaerobic microorganism liquid bacterial agent, the compound anaerobic microorganism liquid bacterial agent, the compound anaerobic microorganism liquid bacterial agent and the compound anaerobic microorganism liquid bacterial agent, the compound anaerobic microorganism liquid bacterial agent, the compound anaerobic microorganism liquid bacterial agent and the compound anaerobic microorganism liquid bacterial agent, and the compound anaerobic microorganism liquid bacterial agent is prepared from the compound anaerobic microorganism liquid bacterial agent and the compound anaerobic microorganism liquid bacterial agent. The microbial inoculum is formed by compounding four strains of synergistic bacteria, the culture process is simple and easy to implement, and when the microbial inoculum is applied to medium-temperature and medium-temperature anaerobic digestion, the conversion rate of cellulose to methane (greater than 40%) and the yield of methane (greater than 120%) can be remarkably improved.

The present invention discloses a device for deeply multi-stage treating industrial wastewater, and the wastewater treatment process steps of the device are as follows: 1) collecting the wastewater through a collecting tank; 2) introducing the wastewater in the collecting tank into a pH adjustment tank, testing the pH value of the sewage in the tank through a pH tester in the pH adjustment tank, and adjusting the pH value to pH 3-5.5; 3) the adjusted wastewater enters an iron-carbon reactor, and iron-carbon micro-electrolysis treatment is carried out on the wastewater in the iron-carbon reactor, and the reaction time is 40-95 minutes; 4) the wastewater treated by the iron-carbon reactor enters a neutralization device to neutralize the wastewater and adjust the pH value of the sewage to 7-9; 5) the neutralized wastewater is introduced into an anaerobic device for anaerobic treatment; 6) the wastewater treated anaerobically is subjected to sedimentation treatment through a sedimentation tank. The present invention can change the flow direction of the wastewater, can not only accelerate the neutralization rate of the wastewater, but also make the wastewater neutralize more evenly; and can prevent a large amount of anaerobic bacteria sludge from being carried inside the discharged clear water.

The invention relates to the technical field of wastewater treatment, and discloses a chemical wastewater treatment system, which is characterized in that an adjusting tank is used for introducing chemical wastewater, adjusting the pH of the wastewater through a pH regulator, and adding suspended solids to obtain pretreated wastewater; the anaerobic reaction tank is used for introducing the pretreated wastewater, adding an anaerobic bacterium agent, carrying out biogas backflow aeration stirring, and regulating and controlling the biogas backflow flow and the stirring intensity in real time through a fuzzy PID algorithm; the aerobic reaction tank is used for introducing anaerobic effluent, adding an aerobic microbial agent, predicting the degradation-resistant COD (Chemical Oxygen Demand) concentration of the effluent by adopting an LSTM (Long Short Term Memory) model, and if a predicted value is greater than 50mg / L, prolonging the hydraulic retention time by 0.5-1h or increasing the dosage of the microbial agent; the hydrogen incinerator is used for heating anaerobic reaction inlet water to 30-35 DEG C through the heat exchanger; the post-treatment unit is used for discharging or recycling aerobic effluent after the aerobic effluent is subjected to membrane filtration and disinfection and reaches the standard; the degradation-resistant COD (Chemical Oxygen Demand) standard-reaching rate of effluent is improved.

The utility model discloses an anaerobic reactor for wine brewing wastewater treatment relates to anaerobic reactor technical field, including reaction box, support leg, rotation water distribution subassembly, blow -off pipe subassembly, marsh gas collection subassembly and blow -off subassembly, the inside installation of reaction box has rotation water distribution subassembly, blow -off pipe subassembly, marsh gas collection subassembly and blow -off subassembly, and the downside of reaction box is provided with four support legs, rotation water distribution subassembly contains rotating cylinder, circular water distribution tray, shower nozzle, scraper, filter plate mounting ring and conical filter plate, and the upper end middle part rotation of reaction box is connected with rotating cylinder, and the lower end of rotating cylinder is provided with circular water distribution tray, and the outside of circular water distribution tray is provided with a plurality of shower nozzles, and this anaerobic reactor for wine brewing wastewater treatment can filter and clean up big granular pollutant, is equipped with water level and temperature double detector simultaneously, monitors the reaction environment in real time, ensures the best activity of anaerobiosis group, and improves the processing efficiency of organic wastewater.

The utility model discloses an anaerobic bacterium feeding device, which belongs to the field of sludge recycling and comprises a moving mechanism and a stirring mechanism, the moving mechanism comprises a second motor and a threaded rod, and the second motor and the threaded rod are mounted on a sludge treatment box; the stirring mechanism comprises a driving gear, a first driven gear, a second driven gear, a first rotating shaft and a second rotating shaft, the stirring mechanism and the anaerobic bacteria liquid outlet are driven to move in the sludge treatment box through movement of the connecting piece, multi-point and uniform feeding of anaerobic bacteria in sludge is achieved, the feeding range is expanded, and the feeding efficiency is improved. The condition that anaerobic bacteria are unevenly distributed in the sludge treatment box is effectively improved, meanwhile, through the design that the two stirring shafts rotate bidirectionally and the stirring blades are staggered, the sludge forms a complex flowing mode in the stirring process, the viscous structure of the sludge can be broken more effectively, full contact and mixing of the anaerobic bacteria and the sludge are promoted, and the sludge treatment effect is improved. And the decomposition efficiency is greatly improved.