22 results about "Anaerobic bacterium" patented technology

The present disclosure provides a method and device for heating sludge anaerobic fermentation to produce biogas by microwaves under the action of a magnetic field, and relates to the technical field of green energy. The method comprises: drying and crushing sludge raw materials and inoculating anaerobic bacteria to form a fermentation mixture; placing the fermentation mixture in a closed reactor equipped with a microwave heating system and an electromagnetic field generating system, forming a uniform temperature field by microwaves and applying a controllable magnetic field to stimulate microbial activity; periodically switching or cooperatively controlling the two systems to maintain the target temperature zone and realize dynamic metabolic reinforcement; detecting the biogas yield and components, and adjusting the energy field parameters to form a closed loop control accordingly. The present scheme can establish a dynamic coupling between energy input and microbial metabolism, make the internal temperature distribution of the system uniform, the reaction rate stable, and the energy utilization rate high, and further make the organic matter degradation more sufficient and the gas production process more continuous, so that the energy efficiency and controllability of the overall anaerobic fermentation can be significantly improved.

The application discloses an oil agent equipment cleaning waste liquid treatment device and method, and relates to the field of oil agent waste liquid treatment. The device comprises an electric field demulsification mechanism in communication with a waste liquid pipe, wherein a pulse electrode is arranged in the electric field demulsification mechanism, a composite reactor is arranged on the downstream side of the electric field demulsification mechanism, an anaerobic bacteria area, a lower grid, a transverse partition plate, an upper grid and an aerobic bacteria area are arranged from bottom to top in the electric field demulsification mechanism, and the electric field demulsification mechanism is further provided with an aeration assembly, a monitoring and shunting system and a gas collecting mechanism. The treatment method comprises the following steps: electric field demulsification pretreatment, aeration, aerobic / anaerobic microbial degradation, multi-parameter monitoring and shunting treatment. According to the application, secondary pollution is avoided through pulse electric field demulsification, pollutants are degraded in stages by using an anaerobic-aerobic linkage circulation structure, real-time monitoring and intelligent shunting are combined, and the treatment efficiency is improved and the operation cost is reduced.

The application discloses a small and micro river ecological landscape restoration system, comprising: a drop weir including a front end suspended in the downstream of a river channel; a stone cage wall for supporting the drop weir; a riprap group for constructing a deep pool-shoal sequence, including riprap blocks for forming a nutrient temporary storage area, and refuge gaps provided between adjacent riprap blocks for small fish and shrimps; and a vegetation tower. The application also discloses a small and micro river ecological landscape restoration method. The small and micro river ecological landscape restoration system and the restoration method make the water body in the river channel fall through the front end to form a water curtain in contact with air, increase the oxygen content in the river channel, inhibit the generation and reproduction of anaerobic bacteria, prevent the water body from being black and smelly, ensure the cleanliness of the water body, and increase the ornamental property. The riprap group facilitates the reproduction and refuge of small fish and shrimps, and the vegetation tower is further beneficial to the long-term stable and sustainable development of the water body ecological landscape system.

The application discloses an iron-based raw material reinforced pig manure and rice straw mixed anaerobic fermentation device, and particularly relates to the technical field of anaerobic treatment, and comprises a fermentation tank, a groove cylinder, a moving block and a cover pressing piece. The upper surface of the fermentation tank is fixedly connected with the groove cylinder. The device further comprises: the moving block is arranged on one side of the groove cylinder; the cover pressing piece is installed on one end of the moving block, and is used for covering and pressing the top end surface of the groove cylinder when the anaerobic bacteria agent is put into the groove cylinder. The application has formed a complete closed loop process from the top cover, automatic positioning to in-situ verification, realizes the mandatory automatic verification before the anaerobic bacteria agent is added, eliminates the operation space of directly adding unqualified anaerobic bacteria agent by personnel violation, greatly improves the anaerobic treatment effect, and solves the problem of adding out of control caused by human subjective negligence, destroys the microbial activity and metabolic balance, and causes the significant decrease of the anaerobic treatment effect.

Disclosed is a bile acid-metronidazole conjugate or a stereoisomer thereof, or a salt thereof, or a solvate thereof, or a prodrug thereof, or a metabolite thereof. The bile acid-metronidazole conjugate is prepared using an esterification reaction of a bile acid compound with hydroxyl group on a metronidazole structure. The synthesis process is simple and easy for industrialization. The bile acid-metronidazole conjugate can effectively inhibit the growth of anaerobic bacteria, especially the growth of Clostridioides difficile and Helicobacter pylori, so that the bile acid-metronidazole conjugate has a significantly improved therapeutic effect in the treatment of infections caused by the anaerobic bacteria.

The utility model discloses a kind of multi-oxygen state vertical flow constructed wetland systems, belong to sewage treatment field, including outer reactor, the inside fixed mounting of outer reactor has inner reactor, the bottom fixed mounting of outer reactor has water inlet pipe, the water inlet pipe is fixedly installed with water distribution pipe in outer reactor one end close, the bottom of outer reactor and inner reactor is filled with bottom layer of goose pebble, the inside of outer reactor is filled with outer filling layer, the top of outer filling layer is filled with top layer of goose pebble, the inside of inner reactor is filled with inner filling layer.The beneficial effects of the utility model are: by setting inner reactor and outer reactor system environment, the bottom of inner reactor continues to water, forms uplink saturated water flow, creates anaerobic environment, dynamic distribution dissolved oxygen, anaerobic zone dominant enrichment anaerobic bacteria, degrades organic matter and releases ammonia nitrogen.

The application discloses a preparation method of a hydrophobic H-SiO2@PNIPAM hybrid microgel stabilized phase inversion type Pickering emulsion and application thereof, and relates to the technical field of nanometer material preparation and biological culture. The application prepares the surface hydrophobic H-SiO2@PNIPAM hybrid microgel particles through a precipitation polymerization method and a sol-gel reaction, then uses the hybrid microgel as an emulsifier to stabilize a W / O type Pickering emulsion, can encapsulate the obligate anaerobe bifidobacterium in the inner part of the emulsion droplets, and realizes efficient culture. The hybrid microgel prepared in the application has strong hydrophobicity, and meanwhile, the temperature sensitivity of the PNIPAM core is reserved, so that the Pickering emulsion stabilized by the hybrid microgel also has temperature sensitivity, and the emulsion can be phase-inverted only by temperature change, so that the inner phase bacteria are released, and rapid separation is realized. Compared with the conventional anaerobe culture method, the method disclosed by the application can realize efficient culture and rapid separation of the bacteria after culture, and has a good application prospect.

The application provides a preparation method of a biomineralization coated bacterium, the biomineralization coated bacterium and application thereof, and relates to the technical field of microbial medicine preparation.The preparation method of the biomineralization coated bacterium comprises the following steps: mixing bacteria and a polyvinylpyrrolidone solution to obtain a bacterium suspension; mixing a CaCl2 solution with the bacterium suspension, and then adding an Na2CO3 aqueous solution; stirring for 0.5-1.5 hours, centrifuging, collecting the precipitate and obtaining the biomineralization bacterium. The method is simple and convenient, and is used for assisting biological interface mineralization through electrostatic interaction to deposit a super-resistant and self-adapting bacterium protection coating. The method is universal for coating various species including obligate and facultative anaerobes. The preparation procedure and the formed coating have negligible influence on the activity of the bacterium, and the bacterium is free from the double invasion of gastric acid and bile acid, so that the activity and quantity of the bacterium reaching the intestinal tract are ensured.

This invention discloses an anaerobic bioreactor based on temperature-sensitive activation and modular integration, and its application method, belonging to the technical field of anaerobic bioreactors. The device includes an anaerobic bag module, a temperature-sensitive integrated gas generation and deoxygenation module, and an anaerobic indicator module. Both the integrated gas generation and deoxygenation module and the anaerobic indicator module are located inside the anaerobic bag module. The integrated gas generation and deoxygenation module includes a non-woven fabric encapsulation package, PNIPAM temperature-sensitive hydrogel, and a gas generation and deoxygenation reaction system. The non-woven fabric encapsulation package includes two stacked chambers. The upper chamber is filled with PNIPAM temperature-sensitive hydrogel in a water-saturated state, and the lower chamber is filled with the gas generation and deoxygenation reaction system. When the PNIPAM temperature-sensitive hydrogel drains, the water permeates from top to bottom into the gas generation and deoxygenation reaction system, activating the reaction. This invention completely eliminates operational time constraints and the need for a 2-8°C low-temperature temporary storage step, avoiding damage to anaerobic bacteria caused by temperature fluctuations.

To provide an improved manufacturing process for use with obligate anaerobic bacteria or other microorganisms that are highly oxygen-sensitive. [Solution] The present invention provides a process for spray freezing or spray freeze-drying under conditions of very low oxygen pressure, such as essentially anaerobic conditions, and in particular, a process for spray freezing obligate anaerobic microorganisms (bacteria) under conditions of very low oxygen levels or essentially anaerobic conditions. Furthermore, the present invention provides products that can be obtained by this process and apparatus that can be used in this process.

The present invention provides a process for spray freezing or spray freeze drying under conditions of very low oxygen pressure, such as under essentially anaerobic conditions, and particularly a process for spray freezing strict anaerobic microorganisms (bacteria) under conditions where the level of oxygen is very low, or essentially anaerobic. Further, the invention provides a product achievable by the processes and an apparatus usable in the process.

The application discloses an anaerobic system nutrient salt adding method based on AI intelligent control, relates to the technical field of industrial wastewater biological treatment, and comprises the following steps: collecting real-time water quality time sequence data and laboratory biochemical true value data, and constructing a multi-modal input vector; inputting an LSTM-Attention model to generate an anaerobic bacteria specific growth rate prediction value and a dynamic dosing weight coefficient; based on the weight coefficient, a double-loop control strategy is executed, the total dosing amount target is optimized in the outer loop, and the instantaneous dosing flow instruction is calculated in the inner loop; the instruction is sent to adjust the dosing amount, and the true value deviation value is calculated; when the deviation value exceeds the threshold value, sensor calibration and model incremental training update are executed. The application solves the problems of blindness in addition and system instability caused by the mismatch between the long lag of the traditional control mode and the fast fluctuation of the water hydraulic load.

This utility model relates to the field of leather wastewater treatment technology, and more particularly to a device for hydrogen sulfide removal in a biogas stripping anaerobic system. The device includes an anaerobic reaction assembly, a gas hood assembly, a gas-liquid separator, a desulfurization tower, and a blower. The anaerobic reaction assembly includes a reaction tank with a first inlet inserted into its side wall. The gas hood assembly includes a stripping gas collection hood fixed inside the reaction tank. Compared to traditional hydrogen sulfide removal devices in biogas stripping anaerobic systems, this utility model, by incorporating a gas hood assembly and a stripping perforated pipe, allows hydrogen sulfide to be stripped through the perforated pipe and collected by the stripping gas collection hood. The hydrogen sulfide is then lifted to the gas-liquid separator for gas-liquid separation and drying, and finally desulfurized by the desulfurization tower. Activating the blower accelerates the biogas flow rate, allowing the biogas to re-enter the reaction tank, stripping perforated pipe, and stripping gas collection hood, thereby reducing the hydrogen sulfide content inside the reaction tank, reducing inhibition of anaerobic bacteria, and thus improving the efficiency of anaerobic treatment of leather wastewater.

This invention discloses a high-purity, multifunctional probiotic powder and its preparation method. Based on the different characteristics of lactic acid bacteria, Bacillus subtilis, and actinomycetes, this invention employs different methods for their respective proliferation. This not only avoids potential mutual interference and contamination during their individual proliferation processes but also achieves high viable cell counts while maintaining high purity, and the high viable cell count is maintained even after mixing. This invention mixes the proliferated bacterial powders in a reasonable ratio, allowing lactic acid bacteria to be the dominant strain while fully utilizing the functional characteristics of each of the three bacteria: lactic acid bacteria are anaerobic, while Bacillus subtilis and actinomycetes are aerobic. The latter can absorb oxygen released by the former, ensuring that in the anaerobic environment of the human intestine, Bacillus subtilis and actinomycetes remain active rather than dormant, generating resistant endospores through synergistic action with lactic acid bacteria.

This invention belongs to the field of microbial culture technology. It provides a method for culturing cariogenic acid-producing anaerobic bacteria and its application. The culture medium comprises the following components in the following proportions: peptone 0.5-5%, sucrose 1-10%, sodium chloride 0.1-0.6%, bromocresol green 0.1-0.3%, bromocresol purple 0.01-0.15%, vitamin C 0.01-0.05%, Tween-80 0.01-0.06%, phenylethanol 0.01-0.25%, and deionized water, forming a liquid culture medium. Based on the specific requirements of bacterial culture, this invention is used for culturing bacteria from teeth and the oral cavity. Peptone is used as the nitrogen source, and sucrose as the carbon source. When bacteria multiply rapidly, they convert sucrose into an acidic substance, changing the pH of the culture medium. In the presence of bromocresol green / bromocresol purple, the color of the culture medium changes, allowing for the determination of bacterial content in the sample and the prediction of the risk of dental caries.

This utility model discloses an anaerobic bacteria cultivation device, specifically relating to the field of anaerobic bacteria cultivation technology. It includes a cultivation chamber and a reaction tank. The cultivation chamber houses the anaerobic bacteria cultivation device, and the reaction tank generates CO2 gas. The cultivation chamber includes a cylindrical body and a top cover. The cylindrical body is open at both ends, and the top cover is detachably and sealed to one end of the cylindrical body. The other end of the cylindrical body is connected to the gas outlet of the reaction tank. The top cover has a gas outlet pipe with a first valve installed on it. This utility model has a simple structure, low cost, and is suitable for small-scale constant-temperature cultivation equipment, promoting the research progress of anaerobic microorganisms.

The present disclosure relates to a composition comprising (a) (i) a Megasphaera elsdenii cell, an anaerobic bacterial cell, or an anaerobic bacterial cell in a trophic state, (ii) at least one carrier and (iii) a core comprising one or more lipids and (b) at least one layer of one or more lipids. In some embodiments, the core is coated by a layer of one or more lipids, and at least 10% of the Megasphaera elsdenii cell, the anaerobic bacterial cell, or the anaerobic bacterial cell in a trophic state is viable in the composition. The present disclosure also relates to feed additives, feeds, premixes, and kits comprising the composition described above.

The application discloses a combined sewage treatment device, and belongs to the technical field of sewage treatment, which comprises a main mechanism for inputting oxygen for aerobic bacteria, a discharge mechanism and two treatment mechanisms for respectively treating sewage by anaerobic bacteria and aerobic bacteria on the main mechanism, which are used for discharging and detecting the sewage; the main mechanism can continuously pass air into the treatment cylinder of the treatment mechanism below, and due to the continuous rotation of the stirring rod, each air outlet can uniformly discharge air under the action of centrifugal force, so that the oxygen in the sewage is uniformly supplemented, and the aerobic treatment effect is good; the two treatment mechanisms respectively treat the sewage by anaerobic bacteria and aerobic bacteria, the treatment effect is good, the bacteria frame can be lifted and rotated, the bacteria group in the bacteria frame is convenient to observe and operate, and the use is convenient; when the discharge mechanism detects the treated sewage, only a certain amount of sewage is located in the detection cylinder, and the detection effect is good.

This invention, entitled "Application of 2,4-Dihydroxybenzoic Acid in Promoting Bacterial Growth," belongs to the field of biomedical technology. The technical problem to be solved is to provide a novel, simple, low-cost substance that can promote the growth of beneficial bacteria. The key technical point is that this invention is the first to discover the application of 2,4-dihydroxybenzoic acid in promoting bacterial growth. Adding 2,4-dihydroxybenzoic acid to a culture system can effectively promote bacterial growth, especially the growth of strictly anaerobic bacteria.

This invention discloses an anaerobic fermentation device and method for Pu-erh tea, belonging to the field of tea processing technology. The device includes a housing, a drum, and a hot air heating device. The outer circumference of the drum has inlet and outlet ports and inlet / outlet gates. The top and bottom of the housing are respectively equipped with a feeding gate and a discharging gate. A hollow tube is fixedly installed at the center of the front end plate of the drum, and several through holes are opened in the rear end plate, with one-way valves installed at the through holes. The inner end of the hollow tube extends towards the central axis inside the drum, and its surface is covered with several air holes. The outer end is rotatably connected to the housing and extends out of the housing, and is connected to the main pipeline via a rotary joint. Three branch pipelines are connected in parallel to the end of the main pipeline. The ends of the three branch pipelines are respectively connected to a vacuum pump, an ultrasonic humidifier, and a pressurized nitrogen device. The method uses nitrogen filling to inhibit aerobic bacteria and dominate the metabolism of anaerobic bacteria. This invention can reduce bitterness and musty taste, allowing flavor substances to accumulate, making it suitable for the fermentation of high-end ripe Pu-erh tea.

The present application relates to the technical fields of microbial culture, and particularly relates to an anaerobic bacteria culture device capable of regulating anaerobic degree and a use method thereof, comprising a vacuum pump, a CO2 gas cylinder, an H2 gas cylinder, an N2 gas cylinder and an anaerobic bottle, the vacuum pump is connected with the anaerobic bottle, each gas cylinder is connected with a proportioner to control the gas proportion in the anaerobic bottle, a culture tube is arranged in the anaerobic bottle, an opening is formed in the anaerobic bottle, a pressing plate for pressing a sealing film is hinged to the anaerobic bottle, and a driving assembly for driving the culture tube to move upward is arranged on the anaerobic bottle. The present application has simple structure, convenient operation, controllable culture environment and low price, and is suitable for small-scale enterprises and laboratory students. The strict anaerobic requirement in the experiment process is achieved through vacuum negative pressure adsorption. The gas proportion is regulated through the proportioner to meet different experimental requirements. The anaerobic degree of the environment can be further determined through the anaerobic indicator arranged in the system.