542 results about "Anaerobic bacterium" patented technology

The present invention provides a bacterium belonging to the genus Bifidobacterium, by which DNA coding for a protein having an antitumor activity or DNA coding for a protein having the activity of converting a precursor of an antitumor substance into the antitumor substance is delivered to tumor tissues specifically under anaerobic conditions thereby expressing the protein encoded by the DNA, as well as a pharmaceutical composition comprising said anaerobic bacterium.

The invention discloses a preparation method of a biological feed additive with nutrition and immunocompetence. The biological feed additive with nutrition and immunocompetence comprises the following components: 0.05 to 0.1% of aerobic bacteria, 0.05 to 0.1% of anaerobic bacteria, 0.3 to 0.5% of fermenting promoter, 5 to 15% of soybean meal, 5 to 10% of molasses, 15 to 25% of DDGS (Distillers Dried Grains with Soluble), 10 to 30% of cassava residue, 20 to 50% of bran meal, 5 to 15% of bran, 5 to 15% of maize cob powder, and 20 to 40% of maize slurry protein. The preparation method comprises the processes of activating bacteria, crushing raw materials, aerobically fermenting for 1 to 2 days, and then anaerobically fermenting for 3 to 7 days. The prepared biological feed additive brings piglet, medium and large pigs and sheep with high nutrition value, is high in palatability, and has effects of accelerating growth, improving intestinal health, controlling diarrhea, controlling constipation of sow, increasing birth weight and weaning weight of the piglet, improving the quality of eggshell and egg yolk, controlling feed-containing faeces, raising the lactation level of dairy cattle, as well as increasing butterfat and protein content.

The invention relates to the field of wastewater treatment, and relates to a biological degradation method of nitrogen-containing heterocyclic compound wastewater. An oxidation state compound is added in the nitrogen containing heterocyclic compound wastewater, an anaerobic environment is formed by micro aeration, and mixed electron acceptors of dissolved oxygen and the oxidation state compound simultaneously exist in the system; an upflow biological aerated filter is used as a main reaction device, an optimal degradation effect of nitrogen-containing heterocyclic compounds is achieved according to different components and concentrations of the nitrogen-containing heterocyclic compounds, the pH is controlled to 7.0-7.5, the concentration of the dissolved oxygen is controlled to 0.05-0.3 mg/L (water discharged from the upper part of the filter), and the range of the oxidation-reduction potential is controlled to 150-50 mv; under an anaerobic aeration condition, with the nitrogen-containing heterocyclic compounds as electron donors and oxygen and oxygen-containing compounds as electron acceptors, under the combined effects of micro aerobic bacteria, facultative anaerobic bacteria, aerobic/anoxic denitrifying bacteria and the like, the nitrogen-containing heterocyclic compounds are completely degraded into carbon dioxide and water.

The invention provides a bioremediation composition for in situ bioremediation of soil and/or groundwater contaminated with hydrocarbons, comprising an adsorbent capable of adsorbing said hydrocarbons, a mixture of facultative anaerobes capable of metabolizing said hydrocarbon under sulfate-reduction conditions, a sulfate-containing compound that releases sulfate over a period of time, and a nutrient system for promoting growth of said anaerobes, wherein said nutrient system includes a sulfide scavenging agent.

The present invention provides systems and methods to isolate microorganisms and optionally their genetic material. The isolation of microorganisms is conducted under anaerobic or other similar conditions to facilitate the preparation of microorganisms that are anaerobes, whether obligate or facultative, as well as microaerophiles. In an alternative format, the isolation of microorganisms is from a carbonaceous substrate which typically hinders or retards isolation therefrom.

Described herein is a method of treatment of cancer or tumor using a modified bacteria or composition comprising the modified bacteria. In certain embodiments, the method is in combination with other treatment. In certain embodiments, the treatment is chemotherapy, radiation therapy, gene therapy, surgery or a combination thereof. The method makes modified facultative anaerobic bacteria into a conditional obligate anaerobe. The modified bacteria are strictly hypoxia regulated and comprise an essential gene expressing cassette. The vectors of this method comprise the essential gene expressing cassette. Also described herein are therapeutic and prophylactic compositions comprising the modified bacteria. The therapeutic and prophylactic compositions contain a purified form of the modified bacteria, while in certain embodiments, they do not contain other strain of microorganisms. The modified bacteria grow within the solid tumor/cancer, retarding its growth and are rapidly eliminated from normal tissues. The solid tumor/cancer includes breast cancer, liver cancer or neuroblastoma.

The present invention relates to one kind of stalk microbe soil conditioner and its preparation process. The stalk microbe soil conditioner is prepared through crushing stalk into powder; steam blasting, adding water, high temperature steam sterilizing and microwave treating; cooling, spraying bacteria seed and mixing with culture liquid; anaerobic and aerobic solid fermentation to obtain cultured matter, and classified culturing fermentation to prepare the stalk microbe soil conditioner. The stalk microbe soil conditioner is used in improving soil, promoting root system growth, decomposing potash and phosphate, raising fertilizer effect, inhibiting harmful bacteria in soil, preventing diseases of crop, increasing crop yield and improving agricultural product quality.

The present invention relates to stalk microbe feed additive and its preparation. After stalk as solid culture medium is crushed, gas exploded, added with water, sterilized, microwave treated and cooled, mixed bacterial seed culture liquid is sprayed for solid fermentation in anaerobic fermenting tank and aerobic fermenting tank to obtain anaerobic bacterial seed and aerobic bacterial seed. Through further fermentation with the anaerobic bacterial seed and aerobic bacterial seed in certain ratio, stalk microbe feed additive is prepared. The stalk microbe feed additive can raise feed converting rate, promote animal's growth, raise animal's disease resistance and produce other positive effects.

The invention discloses a method for treating wastewater in an artificial wetland and a wastewater treating device for implementing the method, wherein the method for treating the wastewater comprisesthe following steps: firstly, taking advantage of plant roots to absorb N, C and P from the wastewater so that part of N, COD, BOD and P can be removed; then utilizing nitrobacterium, aerobes and polyphosphate accumulationg bacteria to remove ammonia, organisms and phosphorus from the wastewater; then, utilizing acidified hydrolytic bacteria and anoxic bacteria to remove macromolecular organisms;and finally utilizing methane bacteria and anaerobes to remove organic acid and micromolecular organisms. In addition, the wastewater treating device comprises an artificial pond. The bottom of the artificial pond is provided with a bearing layer. An active carbon filter layer for filtering and purifying water is arranged above the bearing layer. A bacteria layer for eliminating and decomposing various pollutants in the wastewater is arranged above the active carbon filter layer. A layer of wetland filler is arranged between the active carbon filter layer and the bacteria layer. In the artificial pond, the bottom of the bearing layer is provided with a water draining pipe.

The invention provides a method for treating dyeing wastewater and a dedicated device. The method comprises the following steps of treatment under the automatic control of a central control unit: A, discharging the dyeing wastewater which is uniform quality and uniform quantity into a flocculation precipitating tank, and adding coagulating agent and flocculating agent to the flocculation precipitating tank so as to precipitate the suspended substance and part of organic substances; B, discharging the clear liquid of the wastewater in step A into a hydrolytic acidification tank, and decomposing the macromolecular organic substances in the wastewater into micro molecular substances under the action of facultative anaerobe; C, discharging the wastewater in step B into a contact oxidation tank, feeding air into the contact oxidation tank and decomposing the organic substances in the wastewater under the action of aerobe; D, discharging the wastewater in step C into a membrane bioreactor, and entrapping and decomposing the stale biological membrane slag and the residual organic substances by the membrane bioreactor; and E, sucking the clear water in step D into a clean water tank and discharging the sludge in the membrane bioreactor into the hydrolytic acidification tank or the contact oxidation tank respectively through a return sludge pump by the concentration of the sludge. The invention has the advantages that the treatment cost is low and the treated water is recyclable, thereby helping enterprises to achieve the purposes of energy conservation and emission reduction.

A method for quantitatively testing the water quality in oil field by use of sulfate reducing bacterium (SRB) includes taking 3-5 parallel samples in same dilution gradient, filling them in culture media, putting the MPN culture media on shaker, shaking at 38 deg.C and 180 rpm and respective counting in the fourth and the seventh days.

The present invention provides an integrated produced methane denitriding and phosphorus-sulfur eliminating wastewater treating technique and the equipment thereof, the technique comprises leading the wastewater of the mixing water of wastewater and the reflux water into the high speed anaerobic particle sewage reactor from the bottom, and providing oxygen to the inner part of the reactor to keep the micro-aerobic state in the reactor and facilitate that the contaminant in the water is treated by the degradation of the particle sewage sludge. The present invention provides an environment in which the aerobic bacteria, anaerobium and amphimicrobe coexist and the oxidizing reaction and reducing reaction function together, the COD eliminating (producing methane) and denitriding and phosphorus-sulfur eliminating are simultaneously executed in a reactor, the eliminating efficiency of the contaminant is high and the stay time is short.

The invention provides a preparation method of fruit ferment. The preparation method comprises the following steps: carrying out anaerobic fermentation by inoculating anaerobic bacterium strains, filtering the obtained fermentation products, saving the filtrate (A), keeping on inoculating aerobic bacterium strains to peels and dregs of fruits to carry out aerobic fermentation for 5 to 15 days, then adding liquor, keeping on fermentation for 5 to 10 days, then filtering the fermentation products, condensing the filtrates, then merging the condensed filtrates, adding molasses, inoculating beneficial bacteria to the mixed filtrate, and sealing the mixed filtrate for 10 to 20 days to obtain the environment-friendly ferment. The preparation method can effectively avoid the mildew of fruit peels during the fermentation process, thus the content of nitrite is reduced, the obtained ferment prepared from fruit peels is healthy and unique, at the same time, the ferment content is increased, the function of the ferment is improved, moreover, the wastes (fruit peels) are converted into valuable resources, and the utilization of wastes is promoted.

The invention relates to a large-scale anaerobic fermentation technique of straws and a device thereof. The fermentation technique includes biological pretreatment, anaerobic fermentation and anaerobic bacteria separation of the straws for separating an anaerobic bacteria liquid used for another circulation. The anaerobic fermentation device for implementing the method is composed of a feed device, an anaerobic generator and an anaerobic bacteria separator. The technique and the device do not need nitrogen source during the fermentation process of the straws and realize continuous feed and discharge during the anaerobic process of the straws; the molecular structure of the straws is changed through the mode of biochemistry; a large amount of straw lignin hard to be anaerobically digested is converted into a carbon source which is easily biodegraded; and a waxiness component for protecting the straws is decomposed, thus being capable of leading the anaerobic bacteria to successfully enter the inside of the straws, improving the speed of the degraded straws and being capable of realizing the production with low cost and large scale.

The present invention provides ketolide derivatives, which can be used as anti-bacterial agents. Compounds disclosed herein can be used for the treating or preventing conditions caused by or contributed to by gram positive, gram negative or anaerobic bacteria, more particularly against, for example, Staphylococci, Streptococci, Enterococci, Haemophilus, Moraxalla spp., Chlamydia spp., Mycoplasm, Legionella spp., Mycobacterium, Helicobacter, Clostridium, Bacteroides, Corynebacterium, Bacillus, Enterobactericeae or any combination thereof. Also provided are processes for preparing compounds disclosed herein, intermediates used in their synthesis, pharmaceutical compositions thereof, and methods of treating bacterial infections.

The present invention relates to an isolated anaerobic bacterium Bacteroides sp. for use as a fibro-biotic for livestock. More specifically, the fibro-biotic supplements a fiber diet to Sus scrofa scrofa with the fibro-biotic improving fiber digestion and decreasing fecal output in these livestock. The present invention also relates to the use of the isolated bacterium as a food composition to improve the health of monogastric livestock and as a feed supplement to livestock.

The invention relates to a preparation method and application of a microbial inoculant for remediating poly-halohydrocarbon-polluted soil, belonging to the technical field of soil remediation in environmental engineering. The invention is characterized in that the microbial inoculant containing the quinone-reducing facultative anaerobe Shewanella sp. and poly-halohydrocarbon dehalogenated product facultative aerobic degrading bacterium is prepared by surplus sludge lysis solution preparation and culture medium optimization, and is used for remediating poly-halohydrocarbon-polluted soil. The microbial inoculant has the advantages of wide raw material sources, simple preparation technique and no secondary pollution during soil remediation, can effectively overcome the defect that the high operating cost of the biologically reinforced remediation of poly-halohydrocarbon-polluted soil limits the large-scale application, and has wide application prospects in remediation of soil polluted by poly-chlorine organic substances, poly-bromine aromatic hydrocarbons and poly-fluorine organic substances.

The invention provides a garbage enzyme preparation method utilizing fruit peels. The preparation method comprises the following steps: the fruit peels are inoculated with an anaerobic bacterium for 10 to 20 days of anaerobic fermentation, and a first filtrate is reserved after filtering of the obtained product; fruit peel dregs are then inoculated with an aerobic bacterium for 5 to 15 days of aerobic fermentation, the fermentation continues for 5 to 10 days after white wine is added, and the product is then filtered; filtrates obtained through the two filtering processes are respectively concentrated and then mixed, molasses is added, the mixture is then inoculated with a beneficial bacterium, and garbage enzyme is obtained after 10 to 20 days of sealed storage. The garbage enzyme preparation method provided by the invention has the advantages that the effects of mildew of the fruit peels during fermentation are effectively avoided, so as to reduce the nitrite content, and ensure that the prepared fruit peel enzyme is healthy and unique; meanwhile, the enzyme content in the garbage enzyme is further increased, so as to improve the functional effects of the garbage enzyme; the fruit peels are used as raw materials, so as to achieve the effect of turning waste into wealth, and improve the waste utilization rate.

The method uses the anaerobic glove case and many modified culture medium to screen the anaerobic bacteria which possesses the highly effective character of degrading chlorophenol and acid resistance. The method comprises the following steps: A. screening the anaerobic particle sludge; B. segregation and purifying bacteria; C. primary screening the anaerobic bacteria degrading chlorophenol; D. second screening: segregation and purifying with the solid-liquid culture medium, until the cell shape being the single thallus. The operation is simple, and the theory meaning and economic value are important. The method provides the theory basis for anaerobic biological treatment process of chlorophenol waste water. The method can be used to separate, screen and culture the chlorophenol degradation bacterium, and provide the good screening method for researching chlorophenol degradation rule and interaction between function bacteria.

The invention discloses a screening method and application of a strain for producing 1,3-propanediol, relating to 1,3-propanediol. A screening method and application of clostridium butyricum Gen160 and the strain screening method for producing 1,3-propanediol with waste glycerol are provided. The screening method comprises the steps as follows: adding collected samples to cabbage juice, putting the samples into a water bath after an enrichment culture to kill nonsporeforms, transferring the samples to a clostridium multiplication liquid medium, carrying out an anaerobic culture and then putting the samples into the water bath again, transferring the samples to a fermentation medium, carrying out a high performance liquid chromatograph to analyze fermentation products after the anaerobic selective enrichment culture and selecting positive strains capable of producing 1,3-propanediol; separating out single colonies by a high layer agar column method; carrying out an aerobiotic culture and the anaerobic culture to remove facultative anaerobes and to obtain strict anaerobes, analyzing the fermentation products and selecting the strains to carry out 16S rDNA (ribosomal deoxyribonucleic acid) sequence analysis and identification, wherein the strains are capable of producing 1,3-propanediol and the cultural characteristics and the colonial morphologies confirm to the cultural characteristics of the clostridium butyricum consistently.

Provided herein are ketolide derivatives, which can be used as antibacterial agents. Compounds described herein can be used for treating or preventing conditions caused by or contributed to by gram positive, gram negative or anaerobic bacteria, more particularly against, for example, Staphylococci, Streptococci, Enterococci, Haemophilus, Moraxalla spp., Chlamydia spp., Mycoplasm, Legionella spp., Mycobacterium, Helicobacter, Clostridium, Bacteroides, Corynebacterium, Bacillus, Enterobactericeae, Propionibacterium acnes or any combination thereof. Also provided are processes for preparation of compounds described herein, pharmaceutical compositions thereof, and methods of treating bacterial infections.

The invention relates to a rotary water-distributing type IC (internal circulation) anaerobic reactor. The rotary water-distributing type IC anaerobic reactor comprises a reaction tower, wherein the bottom of the reaction tower is provided with a water inlet, a water discharging opening is formed in the upper part of the reaction tower, and a water introducing and distributing device, a reflux water distributing device, an inner reflux pipe, a first three-phase separator, a second three-phase separator and a gas-water separator are arranged in the reaction tower from bottom to top sequentially; the upper end of the inner reflux pipe communicates with the gas-water separator, and the lower end of the inner reflux pipe is arranged towards the bottom of the reaction tower; the upper part of the first three-phase separator and the upper part of the second three-phase separator are each connected with a biogas collecting pipe which communicates with the gas-water separator, and the water inlet is connected with the water introducing and distributing device which comprises a main connecting pipe; and one end of the main connecting pipe is provided with two or more branch pipes which areradially arranged, each branch pipe is provided with a water inlet hole, and the water inlet holes all face to the counter-clockwise direction or the clockwise direction. In the way, sewage which is injected into the water inlet can be mixed with anaerobic bacteria-containing sludge in the tower evenly, so that the effect of mass transfer between the sewage and anaerobic bacteria is improved.

The invention relates to biochemical treatment technology for unneutralized furfural wastewater. The furfural wastewater enters into an emergency reservoir first; partial furfural wastewater produced during production of furfural each day enters into a regulating reservoir, and the other part of the furfural wastewater enters into the emergency reservoir; the furfural wastewater in the emergency reservoir is extracted and pumped into the regulating reservoir by a temperature adjusting pump, and the temperature of the furfural wastewater in the regulating reservoir is adjusted to be between 35 and 40 DEG C; the furfural wastewater is lifted by a sewage pump into an anaerobic sequencing-batch reactor, and is fully mixed with anaerobe; a large part of organic pollutant is decomposed into methane and water under the physiological action of the anaerobe; when the pH of a solution in the anaerobic sequencing-batch reactor is raised to be between 7.0 and 7.2, operation of a circulating pump and a pulse feeder is stopped for entering into a precipitation stage; after precipitation, the water flows into an intermediate reservoir through a siphonic decanting drainer; and the wastewater in the intermediate reservoir is lifted by an intake pump of a sequencing-batch activated sludge process, enters into a reservoir of the sequencing-batch activated sludge process according to operation rules of the sequencing-batch activated sludge process, is purified, enters into a clean water reservoir, is subjected to strong oxidation treatment of ozone, and is released after reaching the standard.

The invention discloses a glucamylase and an encoding gene thereof. The protein is the protein of the following (a) or (b): the (a) is a protein consisting of amino acid sequences as shown in a sequence 2 inside a sequence table; and the (b) is a protein which is obtained by carrying out the substitution and/or the deficiency and/or the addition of one or a few amino acid residues on an amino acid residue sequence of the sequence 2 inside the sequence table, has a glucamylase function and is derived from the (a). Thermoanaerobacter tengcongensis (culture collection number AS 1.2430T=JCM 11007T) is amplified through a genetic engineering technology to obtain the encoding gene of the glucamylase and the coding gene is transferred into escherichia coli BL21(DE3); and in addition, experimental results show that the glucamylase has higher optimum reaction temperature 10 DEG C higher than that of the glucamylase from aspergillus niger, good heat stability and better industrial application prospect.