256 results about "Biological growth" patented technology

The invention discloses a multi-objective optimization decision control method for shrimp and silkworm polyculture environment parameters, and relates to the technical field of polyculture control. The method comprises the following steps: deploying a water quality sensor network, and combining prawn and nereis growth monitoring equipment; extracting criticality of environmental parameters and biological growth indexes by using historical breeding data and experimental data; constructing a dynamic coupling model, and establishing a dynamic model of the polyculture system based on a differential equation; developing a hybrid optimization algorithm to balance the competition and symbiotic relationship between the prawns and the nereis; and generating a control instruction set based on an optimization result, and adjusting the control equipment through a fuzzy PID controller. According to the invention, intelligent regulation and control of shrimp and silkworm polyculture environment parameters are realized, and economic benefits of shrimp and silkworm polyculture are improved.

The invention provides an intelligent logistics distribution path optimization method under multiple constraint conditions, and relates to the technical field of logistics distribution, and the method comprises the steps: constructing a constrained graph model according to a time window, vehicle information and real-time traffic API data; performing initial path planning by adopting an improved genetic algorithm; performing dynamic path adjustment according to the initial path set and the real-time traffic API data; and in combination with the optimized path set and customer preference information in the order information, screening an optimal solution through a plant rhizome growth optimization algorithm, and then outputting a final path optimization strategy according to a preset rule engine. According to the method, a plant rhizome optimization algorithm is adopted, a biological growth rule is simulated to realize multi-target co-evolution, and customer preference and task migration flexibility are considered while premature convergence is avoided; through flexible adjustment of constraint conditions and algorithm parameters, the method can adapt to more scenes, and can still maintain stable solving capability under the condition of continuous congestion or sharp increase of orders, thereby remarkably improving the anti-risk capability of a logistics system.

A dry eye composition containing hypochlorous acid and a demulcent. The demulcent can be polyvinylpyrrolidoe with a molecular weight of 30-50 kDa. The composition can be applied to the surface of the eye with a dropper device or a spray dispenser. Critically, hypochlorous acid is stable in the dry eye composition and maintains its effectiveness at preventing microbial growth.

The invention relates to the technical field of microbiological detection and image detection, and provides a microwell plate microbiological growth state detection method, system and equipment based on artificial intelligence. The system comprises an image acquisition module, an image segmentation module, a data set construction module, an image data enhancement module, an image classification module, a classification result determination module and a reasoning module, and is used for detecting whether bacteria grow in the microwell plate; or the system comprises an image regression module and a regression error evaluation module and is used for detecting the biomass of the thalli. According to the invention, a self-built photographing device can be adopted to realize on-site direct automatic detection without sampling, so that high-throughput automatic accurate analysis of the growth state of microorganisms in the microwell plate can be conveniently and quickly realized, experiment consumables and biological samples can be saved, the contamination risk can be reduced, and the experiment efficiency can be remarkably improved. Therefore, the working intensity of detection personnel is reduced, and the method has wide application value. Meanwhile, the complexity of detecting the growth state of the microorganisms in the pore plate is reduced, and popularization and application are facilitated.

A liner mat for waste pit management and drilling fluid recycling at well sites is described. The liner mat includes an adsorbent layer and a base layer. The adsorbent layer includes chitosan and polyhydroxyalkanoate (PHA). The base layer includes polylactic acid (PLA). In some cases, the base layer includes hydroxyapatite (HAp). The adsorbent layer contacts the drilling fluid. The adsorbent layer can mitigate microbial growth and adsorbs at least a portion of pollutants from the drilling fluid. The base layer provides structural support and resistance against mechanical stress and tearing.

The invention provides a composite acrylic acid low-surface-energy antifouling coating, a preparation method and application, and belongs to the technical field of marine antifouling materials, the composite acrylic acid low-surface-energy antifouling coating comprises a three-layer composite structure, the three-layer composite structure comprises a bottom layer, a middle layer and a surface layer from bottom to top, the bottom layer is capsaicin-loaded polymethyl methacrylate, and the middle layer is a polymethyl methacrylate surface layer. The bottom layer is made of capsaicin, the middle layer is made of pressure-sensitive adhesive activated by oxygen plasma, the surface layer is made of dihydroxyl-terminated polydimethylsiloxane, the mass ratio of the bottom layer to the middle layer to the surface layer is (23-25): 1: (23-25), and the mass content of capsaicin in the bottom layer accounts for 5.82-5.75% of the total mass; based on the bonding strength change of the pressure-sensitive adhesive generated by oxygen plasma jet and the capsaicin for effectively inhibiting biological growth, polyacrylic acid is used as a self-polishing reservoir to continuously release an environment-friendly antifouling agent; the pressure-sensitive adhesive adheres to the polydimethylsiloxane layer through the adhesive force change generated by oxygen plasma to achieve the low-surface-energy antifouling effect, synergistic gain is achieved, the composite material intervenes into the biological fouling process from multiple aspects, and the composite material copes with the marine biological fouling process jointly.

An adjusting device for a water system of a combined cycle unit comprises a controller, the combined cycle unit, a cooling water tank, a sedimentation filter tank, a medicament reaction tank, a scale inhibitor water tank, a corrosion inhibitor water tank, a sterilization algicide water tank, a diverter pump, a cooling tower, a standby water storage tower, a confluence water pump and a temperature measurement and control device. According to the device, all the parts are connected through the water conveying pipeline, cyclic utilization and dosing treatment of cooling water are achieved, water scale is reduced, the metal corrosion speed is slowed down, and microorganism growth is inhibited. When the temperature of the combined cycle unit exceeds a set threshold value, the temperature measurement and control device can send an electric signal to the controller, the controller controls the standby cooling tower to be started and pumps cooling water into the standby water storage tower to be cooled, and the cooling water is pumped into the combined cycle unit to be cooled after being cooled. And finally, cooling water is pumped back to the cooling water pool through a water pump, and a circulation period is completed.

The invention relates to the technical field of aquaculture, and discloses a growth monitoring system for aquaculture organisms, and the system comprises a data collection module which is used for collecting water quality parameters, organism growth information and external environment data in real time; the data processing module is used for cleaning, denoising, fusing and normalizing the acquired data; the sensitivity analysis module is used for analyzing the influence of water quality parameters on biological growth and identifying the parameters; the water quality growth modeling module is used for establishing a nonlinear relation model of water quality parameters and biological growth; and the abnormity detection module is used for detecting abnormal modes of water quality or biological growth and triggering an alarm. According to the method, the water quality parameters and the biological growth information are collected in real time, the data processing and analysis technology is combined, the breeding environment and the feeding strategy can be dynamically optimized, and therefore the biological growth speed, the feed utilization rate and the overall breeding efficiency are remarkably improved.

The invention relates to the technical field of preparation of natural food preservatives, in particular to a method for preparing a lactobacillus fermentation product by using whey permeate. According to the present invention, the lactic acid bacteria fermentation product is prepared by using the specific plant lactobacillus (Lactobacillus plantarum) LW11 with the preservation number of CGMCC No.34365, such that the good sterilization and antibacterial ability is provided, and the growth of microorganisms can be effectively inhibited; the bacteriostasis test method of the whey permeate leavening can effectively and rapidly test the anticorrosion inhibition effect of the whey permeate leavening.

The invention relates to the technical field of simulation control, in particular to a water quality adaptability simulation control method based on a fishery breeding scene. The method comprises the following steps: acquiring real-time water quality monitoring data, cultured organism growth state data and environmental meteorological data corresponding to a fishery culture water area, performing standardized preprocessing, and constructing a water quality-organism-environment multi-dimensional incidence matrix; constructing a water quality adaptability simulation model by using the water quality-biology-environment multi-dimensional incidence matrix, simulating corresponding water quality parameter evolution processes under different environment variable combinations, and generating a water quality dynamic simulation data set; determining an optimal water quality parameter interval based on the water quality dynamic simulation data set; according to the optimal water quality parameter interval, a dynamic regulation and control strategy is formulated for comparative analysis, and a water quality regulation and control deviation value is generated; based on the water quality regulation and control deviation value, driving water quality regulation equipment corresponding to the fishery breeding water area to execute adaptive control operation and display. According to the invention, the water quality control efficiency of fishery breeding can be improved.

The invention relates to the technical field of meat food safety production monitoring and management, in particular to a digital visual meat food safety production monitoring and management system. Comprising a data acquisition and binding unit used for acquiring environment time sequence data and static physicochemical data associated with a unique identity identification code of each batch of meat products; the environmental stress modeling unit is used for calculating to obtain a comprehensive environmental stress factor; the microorganism growth prediction unit is used for calculating to obtain the total quantity of predicted microorganisms; the dynamic risk assessment unit is used for calculating to obtain a dynamic safety risk index; and the risk early warning and decision support unit is used for generating a risk level signal, and solving based on a preset critical risk threshold to obtain a predictive remaining shelf life so as to support scheduling decision. According to the system, a solid data foundation is laid for dynamic, continuous and prospective microbial risk assessment of the whole production chain, and remarkable technical progress is realized.

The invention relates to the technical field of microbial fermentation, in particular to a microbial fermentation control system based on an intelligent sensor, and the system comprises a data collection module which is used for periodically collecting environment information and reactant information in a fermentation tank; the data analysis module is used for constructing a fermentation coupling model based on the information change condition of reactants in the fermentation tank in the target time period, constructing a microorganism growth curve according to the environment information change condition, and determining a microorganism growth key area based on the microorganism growth curve; the data judgment module is used for determining a microbial fermentation process based on the fermentation coupling model and the microbial growth key area, and judging whether microbial fermentation meets a preset standard or not according to the microbial fermentation process; and the control and adjustment module is used for determining a control and adjustment mode of the fermentation tank based on a judgment result that the microbial fermentation process does not meet the preset standard. The microbial fermentation environment can be accurately controlled, so that the fermentation effect and the fermentation efficiency are improved.

The invention relates to the technical field of aquaculture, and discloses a marine product seedling raising accurate feeding control system which comprises an intelligent recognition module, a data analysis and decision module, an accurate feeding execution module, an environment monitoring module and a remote control and information management module. Through a high-definition camera and a computer vision processing unit of the intelligent identification module, key information such as the body type, the number and the activity state of the fry is accurately identified by adopting a deep learning algorithm. The big data analysis unit is combined with fry growth stages, types and density factors, a machine learning technology and a preset biological growth model are used for accurately calculating the real-time feed demand of each area, and the decision making unit generates an accurate feeding strategy according to the real-time feed demand. And a feeder of the precise feeding execution module realizes timed, quantitative and fixed-point feeding according to the strategy, so that the problem of inaccurate feeding amount caused by experience judgment in a traditional feeding mode is effectively avoided, the feed waste is greatly reduced, and the feed utilization rate is remarkably improved.

The invention discloses a photobioreactor device for microalgae culture, and relates to the technical field of biological growth containers, the photobioreactor device comprises a frame, a mounting rack I, a controller and an incubator shell, a reaction barrel is rotatably arranged in the incubator shell, and a plurality of lantern boxes are sequentially arranged in the reaction barrel from top to bottom; the top of the outer side of each lantern box is provided with a drainage ring, the bottom end of the bottommost lantern box is fixedly provided with a fourth flow guide pipeline, and a plurality of first exhaust pipes used for releasing pressure in the reaction barrel are internally provided with sampling assemblies. Under the conditions that algae cultivation is not affected and waste gas leakage is little, algae samples are obtained from different positions in the photobioreactor device for multiple times, and the culture effect of the photobioreactor device can be conveniently detected.

The invention belongs to the field of field remediation functional materials, and particularly relates to a preparation method and application of a chelating iron agent for remediation of organic contaminated soil. The preparation method comprises the following steps: firstly, preparing a chelating agent aqueous solution, adding ferrite under a continuous stirring condition to carry out chelation reaction, then adding organic acid to carry out stabilization treatment, finally adding an activating auxiliary agent fulvic acid, uniformly mixing, and adjusting the pH value of the solution to a required value to obtain the chelating iron agent. The chelating iron agent can be used for activating an oxidizing agent during in-situ / ex-situ remediation of an organic polluted site, and has the advantages that the chelating iron agent is environment-friendly, the ferrous ion stability is good, the contact reaction with the oxidizing agent is mild and controllable, iron precipitates are not easy to form, and the growth and metabolic activity of indigenous organisms are stimulated.

The invention discloses an ecological restoration device for promoting assimilation and purification of plankton by direct-drive rotary oxygenation in the field of ecological restoration, which comprises an aeration platform, a purification unit, a floating bed for planting aquatic plants and a carrier filler for microbial adsorption, an energy storage unit, a water supply unit, a water supply unit and a water supply unit, comprising a vertical wind driven generator, a solar power generation panel and an energy storage battery, and the control unit comprises a controller and a dissolved oxygen monitoring probe used for monitoring dissolved oxygen in a water body; the energy storage battery supplies energy to the control unit and the air blower. The invention provides an ecological restoration device for promoting assimilation and purification of plankton through direct-drive rotary oxygenation, the device can be directly driven to rotate by using reaction torque of aeration, the device not only can reduce loss of energy conversion, but also can recover energy of aeration airflow, and a controller intelligently controls oxygenation and aeration and adjusts the growth microenvironment of aquatic organisms, so that the ecological restoration device is suitable for ecological restoration of aquatic organisms. The assimilation purification effect is achieved.

The present application relates to a preservative-free anti-aging soothing composition containing high mountain plant extract and multiple fermentation filtrate, belonging to the technical field of cosmetics. The composition comprises high mountain plant extract complex, multiple fermentation filtrate, anti-aging active and vegetable oil. The organic acids in the multiple fermentation filtrate form a natural buffer system, maintaining a weakly acidic environment to inhibit microbial growth; at the same time, the natural components in the fermentation filtrate provide protection for nicotinamide mononucleotide and ergothioneine, delaying their degradation. The present application does not need to add additional preservatives, and has the functions of anti-aging, soothing and self-preserving.

An estimation device 1 comprises: a model generation unit 10 that, in each time step, calculates an OBM value including the growth rate of marine microorganisms using an ocean physical value calculated from an ocean physical model and an ocean biogeochemistry model (OBM), and trains a learning model for estimating the growth rate of marine microorganisms in each time step from the relationship between the ocean physical value and the OBM value in each time step; and an estimation unit 20 that, in each time step, calculates an OBM value other than the growth rate of the marine microorganism using the ocean physical value calculated from the ocean physical model and the OBM, and uses the learning model to estimate the growth rate of the marine microorganism from the ocean physical value and the OBM value other than the growth rate of the marine microorganism.

This invention discloses a method and system for monitoring and assessing the marine aquaculture environment using IoT big data. The method includes: collecting multi-dimensional environmental parameters such as dissolved oxygen, nitrogen and phosphorus nutrients, and pH value through IoT sensing devices; performing parallel reception and status verification through a high-concurrency marine environmental data processing platform; using a multi-dimensional time-series data adaptive clustering algorithm to perform time-series segmentation and similarity clustering of the parameters; quantitatively assessing water quality status based on the clustering results using a eutrophication assessment model; and then dynamically predicting stress intensity and duration using an environmental stress response prediction model, combined with parameters adapted to the growth cycle of aquaculture organisms. The platform integrates the assessment and prediction results to generate a structured report including parameter change trajectories, level indicators, and predicted trend curves, achieving precise monitoring and assessment of the marine aquaculture environment throughout the entire process, providing reliable technical support for aquaculture environment regulation.

The invention is suitable for the technical field of food packaging materials, and provides a biodegradable lactic acid-based food packaging composite material and a preparation method thereof.The composite material is prepared from polylactic acid, polycaprolactone, tartaric acid-cysteine carbon dots, a bio-based plasticizer, an organic small molecule modifier and modified aerogel; tartaric acid-cysteine carbon dots and polycaprolactone are added to synergistically enhance the mechanical property, barrier property, antibacterial property and antioxidant property of the polylactic acid material; by adding the bio-based plasticizer, the processability and flexibility of the PLA are further improved; by adding the small organic molecule modifier, the dispersity of the carbon dots in a PLA matrix is improved, and the mechanical property, the oxidation resistance and the antibacterial property of the material are further enhanced; by adding the modified aerogel, excellent barrier property and adsorption capacity are provided, the mechanical property of the material is enhanced, and plant-derived antibacterial factors are uniformly distributed in aerogel pores, so that the growth of microorganisms is effectively inhibited.

The present invention discloses a method and system for tracing the entire agricultural industrial chain based on big data, specifically relating to the fields of agricultural Internet of Things and intelligent decision-making technologies, and is used to solve the problems of broken causal chains, mispositioning of damage-causing factors, and insufficient traceability credibility in existing agricultural traceability systems due to the neglect of the irreversibility of biological growth; by dividing time series data segments based on agronomic growth stages, restoring the time series law of crop growth, and dynamically marking the time window of key abnormal events to lock the damage-causing action interval; combining the phase space reconstruction technology to map quality inspection and transportation parameters into system trajectories, and screening the target growth stages associated with biological dynamic laws; through contribution weight analysis and stress resistance data fusion, identifying the main damage-causing factors and their synergistic relationships; finally, dynamically correcting the traceability path based on historical verification results and optimizing the thresholds and weights of the agronomic rule base, significantly improving the accuracy and credibility of tracing the entire agricultural industrial chain.

The invention discloses a light durable liquid cooling plate based on an aluminum foil material and a manufacturing process thereof. The manufacturing process comprises the steps of preparing a material sheet, forming a runner through cold stamping, folding and sealing and the like. The liquid cooling plate is made of a three-layer composite material composed of polypropylene (PP), aluminum foil and nylon, and compared with a traditional pure metal liquid cooling plate, the liquid cooling plate has the advantages of being soft in attachment, good in surface deformation and free of being coated with heat dissipation glue, and the heat dissipation effect is remarkably optimized. The PP layer is resistant to bacteria and corrosion, so that the service life of the liquid cooling plate is prolonged to 10-15 years, and the problems that a traditional metal plate is prone to corrosion, liquid leakage and biological growth are solved. The design is light in weight, low in cost, particularly suitable for mobile electronic equipment such as aircrafts and electric automobiles, high in automation degree of the production process and suitable for batch manufacturing. Due to the ductility and convenient sealing characteristic of the composite material, the liquid cooling plate is more stable in structure and reliable in packaging, and the modern requirements for high efficiency, light weight, durability and heat dissipation are fully met.

The application discloses a curcuma, lemon and green tea extract / chitosan composite preservative film and a preparation method and application thereof. The composite preservative film comprises a chitosan matrix and a composite extract composed of curcuma extract, lemon extract and green tea extract at a weight ratio of 1:1:1. The three kinds of natural plant extracts are prepared by ultrasonic-assisted extraction or reflux extraction respectively, and the composite preservative film is prepared by mixing the chitosan solution, centrifugation, pouring and drying. The composite preservative film is used for the preservation packaging of grouper fillets, can significantly inhibit the growth of microorganisms, delay fat oxidation and protein corruption under 4 DEG C cold storage conditions, and prolongs the shelf life of the grouper fillets to more than 12 days. The synergistic effect of the three kinds of plant polyphenol components is utilized, the preservation effect is improved, safety, non-toxicity, green environmental protection are ensured, the preparation process is simple, the cost is low, and the application is suitable for the field of aquatic product preservation.

A general photobioreactor microbial growth rate prediction model and method thereof, the prediction model comprising a pretreatment module, a fluid dynamics calculation module, a light transmission module and a microbial growth rate mechanism module; first, a physical model of the photobioreactor is obtained; second, according to the obtained physical model and the calculation region, mesh division is carried out to construct the pretreatment module; then, the initial value of the growth parameter of the microbial particles in the photobioreactor is calculated; finally, the microbial growth rate in the photobioreactor is calculated and predicted through a coupled model of computational fluid dynamics, light intensity transmission law and microbial growth mechanism; the present application is based on physical modeling, and the computational fluid dynamics, light intensity transmission and microbial growth mechanism are coupled, thereby expanding the generality of the growth rate prediction; and the model can more accurately predict the microbial growth rate in any photobioreactor.

The invention provides an antibacterial peptide-perfume bonded photoresponse anterior perfume body and application thereof. The anterior perfume body is prepared by reacting hydroxyl or carboxyl of alcohol or carboxylic acid perfume molecules with (5-amino-2-nitrophenyl) methanol and then bonding antibacterial peptide. The prepared antibacterial peptide-perfume bonded photoresponse front perfume body has good photoresponse release performance, the perfume release time can be prolonged, the perfume retention durability can be improved, the perfume utilization rate is remarkably increased, a traditional passive release mode is broken through, and active regulation and control are achieved; high-efficiency broad-spectrum antibacterial activity is achieved, the front incense body is endowed with antibacterial performance through the bonded antibacterial peptide, and microbial growth is effectively inhibited; in addition, amino groups, amide groups and other groups of the antibacterial peptide improve the water solubility of the front incense body, and the application range of the front incense body is further widened. The preparation process has the advantages of easily available raw materials, simple synthesis steps, mild conditions and high yield, can realize large-scale production by simple synthesis of commercially available raw materials under mild conditions, and has wide market prospects and economic benefits.

The invention discloses a preparation method of a low-temperature plasma modified chitosan nano-composite membrane and application of the low-temperature plasma modified chitosan nano-composite membrane in fish preservation, the composite membrane is prepared by taking chitosan as a matrix, and coating curcumin / quercetin / zein nano-particles (ZCQC NPs) with chondroitin sulfate treated by low-temperature plasma (CP). The mechanical property (the tensile strength reaches 61.96 Mpa, and the elongation at break reaches 41.90%), the moisture resistance (the water vapor transmission rate is reduced by 42%, and the oxygen transmission rate is reduced by 54%) and the antioxidant activity (the DPPH removal rate is 78.06%, and the ABTS removal rate is 63.40%) of the film are remarkably improved. In the application of fresh keeping of fish meat, the film can effectively inhibit microbial growth and lipid oxidation. The invention provides an efficient and degradable solution for active packaging of aquatic products.

The invention belongs to the technical field of microbiological detection, and particularly relates to a high-throughput microbiological growth detector. Comprising a loading platform which is used for arranging a culture container; the mechanical shaking device comprises a mounting platform, a first driving motor, a transmission wheel, a transmission rod, a first transmission track and a second transmission track, the first driving motor is arranged at the upper end of the mounting platform, the transmission wheel is connected with an output shaft of the first driving motor, and the lower end of the transmission rod is eccentrically connected with the transmission wheel; the upper end of the transmission rod is rotationally connected with the loading platform, the first transmission track is vertically distributed, and the second transmission track is horizontally distributed. The first driving motor is used for driving the transmission wheel to rotate, so that the upper end of the transmission rod is driven to move relative to the first transmission track and the second transmission track, the loading platform can be driven to shake in the vertical direction and the horizontal direction, and a culture solution and microorganisms in the culture container can be uniformly mixed.

The utility model discloses a rotary oscillation shaker for anaerobic culture, which relates to the technical field of microbial culture and comprises a casing with a double-layer toughened visual glass door, a netlike tray is rotatably connected inside the casing through a rotating shaft, and a plurality of centrifuge tube placing grids are arranged at the top of the netlike tray. A protective cover matched with the net-shaped tray is arranged at the top of the net-shaped tray, a servo motor is fixedly installed on the inner wall of the machine shell, and a transmission belt for transmission is arranged between the output end of the servo motor and a rotating shaft connected with the net-shaped tray. A horizontal vibrator is fixedly installed at the bottom end in the machine shell. According to the technical scheme provided by the utility model, a composite motion mode combining rotation and oscillation is realized, the mixing uniformity of cultures is improved, so that the anaerobic culture effect is improved, and meanwhile, the temperature and humidity in the box body can be regulated and controlled by the dual-system cold and hot control system, so that the culture environment is ensured to meet the optimal conditions for the growth of anaerobic organisms.

The present application relates to food preservation technology field, propose a kind of additive influence analysis method on goose meat storage quality, steps include: after pretreatment, goose meat is placed in pickling liquid containing additive and pickled;After pickling, halogenation, vacuum packaging and secondary boiling water sterilization are carried out, and stored in cold storage condition;During storage, total number of colonies, pH value, TVBN value, TBARS value, color difference, texture characteristics, sensory evaluation score and volatile flavor substances are detected periodically.The present application evaluates the influence of different additives on the storage quality of marinated goose through multi-dimensional index system, and the results show that: composite natural preservative can significantly inhibit microbial growth and fat oxidation, extend the shelf life to more than 35 days, and improve texture and color;Fresh spices and hot reaction goose meat essence give marinated goose rich layered flavor and prominent spicy characteristics respectively.The present application provides experimental basis for the application of natural composite additives in marinated goose preservation and flavor control, and can guide industrial production.

The invention belongs to the technical field of aquaculture biological growth, and particularly relates to a gene for regulating fish growth, application and a regulating method. The gene for regulating the growth of the grass carp is a myogenesis inhibition gene mdfi of a myogenesis regulatory factor (MRFs) family; the base sequence of the gene is shown in SEQ ID NO: 1. The invention discloses a method for promoting proliferation of fish muscle cells. The expression level or protein activity of mdfi genes in grass carp muscle cells is inhibited. The inhibiting effect of the mdfi gene in the muscle growth of the grass carp is systematically clarified for the first time, the individual growth of the grass carp can be effectively promoted by establishing an mdfi gene knockout technology, and a new technical means and a new gene target are provided for the genetic breeding of the grass carp.