Control method for efficient culture of photosynthetic organisms

Abstract

The invention relates to a control method for efficient culture of photosynthetic organisms, which realizes efficient growth of phycomycetes photosynthetic organisms by intelligently adjusting illumination and culture environment temperature. The control method for the temperature condition of the culture environment comprises the following steps: (1) monitoring the temperature in real time, regularly reading data of a temperature sensor, and transmitting the data to a central control unit; (2) setting a corresponding difference allowable threshold value according to the temperature parameter, wherein the threshold value is correspondingly set according to different biological growth conditions; and (3) after the optimal temperature growth condition is obtained, generating a specific threshold value corresponding to the current temperature environment, converting the threshold value into a control instruction, transmitting the control instruction to a temperature control unit, correspondingly controlling the temperature in the culture equipment, and carrying out constant monitoring on the temperature. By intelligently adjusting illumination and controlling the temperature of the culture environment, environment parameters can be more accurately controlled, it is ensured that photosynthetic organisms are always in the most suitable growth conditions, and the growth speed and quality of the photosynthetic organisms are improved.

Description

Technical Field

[0001] The invention relates to a control method for high-efficiency cultivation of photosynthetic organisms, belonging to the technical field of bacteria and algae cultivation. Background Art

[0002] Photosynthetic organisms have broad application prospects in the fields of bioenergy, biopharmaceuticals, food additives, etc. However, when cultivating bacteria and algae in the laboratory, the existing culture system has problems such as uneven lighting, unstable temperature control, insufficient nutrient supply, and relies heavily on manual recording, regulation and operation by operators, resulting in low growth efficiency and low yield of photosynthetic organisms, and is prone to errors and misoperation.

[0003] CN 206282165U discloses an intelligent digital monitoring and cultivation device for spirulina, comprising a monitoring host, a wireless network terminal and a control execution terminal, wherein the control execution terminal is connected to the monitoring host via the wireless network terminal. As for how to achieve accurate control of light and ambient temperature, there is a lack of theoretical support for the control of light and ambient temperature.

[0004] In view of the above problems, it is necessary to develop a control method and system for the efficient cultivation of photosynthetic organisms that can accurately control factors such as light and temperature that affect the efficient cultivation of photosynthetic organisms. Summary of the invention

[0005] The purpose of the present invention is to provide a control method for the efficient cultivation of photosynthetic organisms in order to overcome the problems of uneven illumination, unstable temperature control, insufficient nutrient supply, and low growth efficiency and yield of photosynthetic organisms in existing culture systems.

[0006] The technical solution implemented by the present invention is as follows: a control method for efficient cultivation of photosynthetic organisms, wherein the method realizes efficient growth of photosynthetic organisms of algae and fungi by intelligently adjusting light and cultivation environment temperature; The control method of intelligently adjusting the illumination is as follows: The installed light sensor performs real-time monitoring inside and outside the culture container, and reads the light sensor data every 30 minutes. The light control unit determines and calculates the light conditions according to the following formula: ; in, is the target light intensity; For the optimal light intensity; is the current ambient light intensity; The number of dynamic changes monitored; The algebraic value of light attenuation during the current reading time is obtained by the light control unit after comprehensively judging the value curve of the light sensor's monitoring change amplitude; It is the light regulation coefficient. The corresponding parameters are set according to different organisms and need to satisfy 0 ≤≤ 1. After the corresponding light intensity is obtained, the data is transmitted to the controller in the light module, and the light intensity of the LED lamp in the culture container is adjusted by the controller.

[0007] The control method of the culture environment temperature is as follows: According to the collected data provided by the temperature sensor and the final temperature control numerical expression, the real-time monitoring, difference calculation, threshold comparison, optimal growth temperature prediction and temperature condition control of the temperature in the culture container are realized, ensuring the stability and suitability of the temperature environment; ; in, is the final temperature control value; is the error signal; is the proportional gain value; is the integral gain value; is the differential gain value.

[0008] The control method steps of the culture environment temperature conditions are as follows: (1) Install temperature sensors inside and outside the culture container to monitor the temperature in real time, read the data of the temperature sensor regularly, and transmit it to the central control unit. The acquired data is defined as initial data.

[0009] (2) The data from the temperature sensor is read once every hour. After each data update, it is compared with the initial data to obtain the data difference. The central control unit then memorizes the difference and marks the time period when the difference exists. It obtains the standard temperature parameters for this time period and sets the corresponding difference allowable threshold based on the temperature parameters. This threshold is set according to different biological growth conditions. When the difference exceeds the specified threshold twice, the central control unit will establish a photosynthetic organism growth model and predict the optimal temperature growth conditions based on historical data.

[0010] (3) After obtaining the optimal temperature growth conditions, a specific threshold corresponding to the current temperature environment is generated, and this threshold is converted into a control instruction and transmitted to the temperature control unit to control the temperature inside the culture equipment accordingly and monitor the temperature constantly.

[0011] A photosynthetic organism efficient culture system comprises an illumination module, a gas supply module, a nutrient supply module, a stirring and mixing module, a biosafety module, a wastewater treatment module and a bio-growth monitoring and control module.

[0012] The lighting module uses high-efficiency LED lights to provide a spectrum of suitable wavelengths to meet the growth needs of photosynthetic organisms. It supports intelligent adjustment of light and cycle through a lighting control unit and an algorithm to simulate natural lighting conditions and promote photosynthesis and growth.

[0013] The gas supply module introduces carbon dioxide into the culture solution through a gas diffuser to improve the efficiency of photosynthesis; and uses intelligent gas exchange to remove excess oxygen in the culture solution to prevent oxygen from inhibiting photosynthesis.

[0014] The nutrient supply module allows the nutrient solution to circulate in the culture container through a pump and a pipeline to ensure uniform distribution of nutrients.

[0015] The stirring and mixing module uses a bubble stirrer to ensure uniform distribution of photosynthetic organisms in the culture solution, avoid sedimentation and agglomeration, and promote mixing of gas and nutrients in the culture solution through bubbles.

[0016] The biosafety module needs to be equipped with sterile operating equipment and filters to carry out detailed management and control of the nutrient solution and remove impurities and harmful substances in the culture solution.

[0017] The wastewater treatment module treats the wastewater generated during the bacterial and algal biological cultivation process.

[0018] The biological growth monitoring control module comprises a central control unit, a light control unit, a temperature control unit and a monitoring control unit; the light control unit comprises a light sensor, the temperature control unit comprises a temperature sensor, and the monitoring control unit comprises a pH sensor, a dissolved oxygen sensor and a conductivity sensor; the central control unit is a microprocessor, connected to the sensors of each control unit, receives signals sent by each sensor and performs analysis, processing and calculation; according to the light intensity signal provided by the light sensor, the central control unit controls the illuminance of the LED lamp; according to the ambient temperature signal provided by the temperature sensor, the central control unit adjusts the temperature and air volume of the cold air system to control the biological culture environment temperature; according to the oxygen content signal of the culture solution provided by the dissolved oxygen sensor, the central control unit issues an instruction to adjust the amount of carbon dioxide introduced into the culture solution by the gas diffuser; according to the information provided by the pH sensor and the conductivity sensor, the central control unit determines the pH and pollution degree of the water, issues an instruction to process the water quality of the culture solution, and adjusts the pH, hardness or ion concentration of the culture solution.

[0019] The beneficial effects of the present invention are that the present invention reduces the frequency of manual intervention and improves the efficiency of experimental operations by automatically adjusting parameters such as lighting, temperature, and gas supply; the automated system can continuously record experimental data, reducing errors and workload in manual recording, making the data more accurate and complete, ensuring that the operating steps and conditions of each experiment are consistent, and improving the repeatability and reliability of the experimental results.

[0020] The present invention can more accurately control environmental parameters through the control method of intelligently adjusting light and culture environment temperature, ensure that photosynthetic organisms are always in the most suitable growth conditions, dynamically adjust parameters according to real-time data, adapt to environmental changes, and improve the growth rate and quality of photosynthetic organisms. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 A flow chart of a control method for adjusting temperature conditions of the present invention; Figure 2 This is a schematic diagram of the efficient cultivation system for photosynthetic organisms; Figure 3 This is a schematic diagram of the biological growth monitoring and control module. DETAILED DESCRIPTION

[0022] This embodiment provides a photosynthetic organism efficient cultivation system, such as Figure 2 As shown, it includes a lighting module, a gas supply module, a nutrient supply module, a stirring and mixing module, a biosafety module, a wastewater treatment module and a biological growth monitoring and control module.

[0023] The lighting module in this embodiment uses high-efficiency LED lamps to provide a spectrum of suitable wavelengths to meet the growth needs of photosynthetic organisms. The controller and algorithm support intelligent adjustment of light intensity and cycle to simulate natural lighting conditions and promote photosynthesis and growth. The temperature control unit maintains a stable temperature in the culture environment through an air cooling system to avoid the impact of temperature fluctuations on photosynthetic organisms, monitors temperature changes in real time, and automatically adjusts the heating or cooling device.

[0024] The gas supply module in this embodiment introduces carbon dioxide into the culture solution through a gas diffuser to improve the efficiency of photosynthesis, and uses intelligent gas exchange to remove excess oxygen in the culture solution to prevent oxygen from inhibiting photosynthesis.

[0025] The nutrient supply module in this embodiment allows the nutrient solution to circulate in the culture container through a pump and a pipeline to ensure uniform distribution of nutrients.

[0026] The stirring and mixing module in this embodiment uses a bubble stirrer to ensure uniform distribution of photosynthetic organisms in the culture solution, avoid sedimentation and agglomeration, and promote mixing of gas and nutrients in the culture solution through bubbles.

[0027] The biosafety module in this embodiment needs to be equipped with at least aseptic operation equipment and filters to perform detailed management and control of the nutrient solution and remove impurities and harmful substances in the culture solution.

[0028] The wastewater treatment module in this embodiment uses energy-saving equipment to reduce energy consumption, reduce carbon emissions, and treat the wastewater generated during the cultivation process.

[0029] like Figure 3 As shown, the biological growth monitoring control module in this embodiment includes a central control unit, a light control unit, a temperature control unit and a monitoring control unit; the light control unit includes a light sensor, the temperature control unit includes a temperature sensor, and the monitoring control unit includes a pH sensor, a dissolved oxygen sensor and a conductivity sensor; the central control unit is a microprocessor, which is connected to the sensors of each control unit, receives the signals sent by each sensor and performs analysis, processing and calculation; according to the light intensity signal provided by the light sensor, the central control unit controls the illumination of the LED lamp; according to the ambient temperature signal provided by the temperature sensor, the central control unit adjusts the temperature and air volume of the cold air system to control the biological culture environment temperature; according to the oxygen content signal of the culture solution provided by the dissolved oxygen sensor, the central control unit issues an instruction to adjust the amount of carbon dioxide introduced into the culture solution by the gas diffuser; according to the information provided by the pH sensor and the conductivity sensor, the central control unit determines the pH and pollution degree of the water, issues an instruction to process the water quality of the culture solution, and adjusts the pH, hardness or ion concentration of the culture solution.

[0030] The control method of intelligently adjusting the lighting in this embodiment is as follows: The installed light sensor performs real-time monitoring inside and outside the culture container, and reads the light sensor data every 30 minutes. The light control unit determines and calculates the light conditions according to the following formula: ; in, is the target light intensity, For the optimal light intensity, is the current ambient light intensity, is the number of dynamic changes monitored, It is the algebraic value of light attenuation during the current reading time. This value is determined by the light control unit after comprehensively judging the value curve of the light sensor's monitoring change amplitude. It is the light adjustment coefficient. The corresponding parameters are set according to different organisms and need to satisfy 0 ≤≤ 1. After the corresponding light intensity is obtained, the data is transmitted to the controller in the light module to achieve automatic adaptive adjustment.

[0031] The control method of the culture environment temperature in this embodiment is as follows: According to the collected data provided by the temperature sensor and the final temperature control numerical expression, the real-time monitoring, difference calculation, threshold comparison, optimal growth temperature prediction and temperature condition control of the temperature in the culture container are realized, ensuring the stability and suitability of the temperature environment; ; in, is the final temperature control value; is the error signal; is the proportional gain value; is the integral gain value; is the differential gain value.

[0032] like Figure 1 As shown, the control method steps of the culture environment temperature conditions are: (1) Install temperature sensors inside and outside the culture container to monitor the temperature in real time, read the data of the temperature sensors regularly, and transmit them to the central control unit. The acquired data is defined as initial data; (2) The temperature sensor data is read once every hour. After each data update, it is compared with the initial data to obtain the data difference. At this time, the central control unit memorizes the difference and marks the time period when the difference exists. The standard temperature parameters under this time period are obtained, and the corresponding difference allowable threshold is set according to the temperature parameters. This threshold is set according to different biological growth conditions. When the difference exceeds the specified threshold twice, the central control unit will establish a photosynthetic biological growth model and predict the optimal temperature growth conditions based on historical data; (3) After obtaining the optimal temperature growth conditions, a specific threshold corresponding to the current temperature environment is generated, and this threshold is converted into a control instruction and transmitted to the temperature control unit to control the temperature inside the culture equipment accordingly and monitor the temperature constantly.

[0033] The embodiments of the present invention reduce the factors of human intervention and improve the efficiency of experimental operation by automatically adjusting parameters such as light, temperature, and gas supply for the growth environment of photosynthetic organisms. The control unit can continuously record experimental data, reduce the errors and workload of manual recording, make the data more accurate and complete, ensure that the operating steps and conditions of each experiment are consistent, improve the repeatability and reliability of the experimental results, and at the same time more accurately control the environmental parameters to ensure that the photosynthetic organisms are always in the most suitable growth conditions. The parameters are dynamically adjusted according to real-time data to adapt to environmental changes and improve the growth rate and quality of photosynthetic organisms.

Claims

1. A control method for a photosynthetic organism efficient culture system, characterized in that: The method achieves efficient growth of algae and fungi photosynthetic organisms by intelligently adjusting light, culture environment temperature, and supply management of nutrient solution and CO2; The control method of intelligently adjusting the illumination is as follows: The installed light sensor performs real-time monitoring inside and outside the culture container, and reads the light sensor data every 30 minutes. The light control unit determines and calculates the light conditions according to the following formula: ； in, is the target light intensity; For the most suitable light intensity; is the current ambient light intensity; The number of dynamic changes monitored; The algebraic value of light attenuation during the current reading time is obtained by the light control unit after comprehensively judging the value curve of the light sensor's monitoring change amplitude; is the light regulation coefficient, which is set according to the corresponding parameters of different organisms and needs to satisfy 0 ≤≤ 1. After obtaining the corresponding light intensity, the data is transmitted to the controller in the light module, and the light intensity of the LED lamp in the culture container is adjusted by the controller; The control method of the culture environment temperature is as follows: According to the collected data provided by the temperature sensor and the final temperature control numerical expression, the real-time monitoring, difference calculation, threshold comparison, optimal growth temperature prediction and temperature condition control of the culture container are realized, ensuring the stability and suitability of the temperature environment; ； in: is the final temperature control value; is the error signal; is the proportional gain value; is the integral gain value; is the differential gain value.

2. The control method of a photosynthetic organism efficient cultivation system according to claim 1, characterized in that: The steps of the temperature control method are as follows: (1) Install temperature sensors inside and outside the culture container to monitor the temperature in real time, read the data of the temperature sensors regularly, and transmit them to the central control unit. The acquired data is defined as initial data; (2) The temperature sensor data is read once every hour. After each data update, it is compared with the initial data to obtain the data difference. At this time, the central control unit memorizes the difference and marks the time period when the difference exists. The standard temperature parameters under this time period are obtained, and the corresponding difference allowable threshold is set according to the temperature parameters. This threshold is set according to different biological growth conditions. When the difference exceeds the specified threshold twice, the central control unit will establish a photosynthetic biological growth model and predict the optimal temperature growth conditions based on historical data; (3) After obtaining the optimal temperature growth conditions, a specific threshold value corresponding to the current temperature environment is generated, and this threshold value is converted into a control instruction and transmitted to the temperature control unit to control the temperature inside the culture equipment accordingly and monitor the temperature constantly.

3. A system for implementing the control method of a photosynthetic organism efficient cultivation system as claimed in any one of claims 1 to 2, characterized in that: The system includes an illumination module, a gas supply module, a nutrient supply module, a stirring and mixing module, a biosafety module, a wastewater treatment module and a biological growth monitoring and control module; The illumination module uses high-efficiency LED lamps to provide a spectrum of suitable wavelengths, and supports intelligent adjustment of illumination and cycle through an illumination control unit and an algorithm to meet the growth needs of photosynthetic organisms; The gas supply module introduces carbon dioxide into the culture solution through a gas diffuser to improve the efficiency of photosynthesis; The nutrient supply module circulates the nutrient solution in the culture container through a pump and a pipeline to ensure uniform distribution of nutrients; The stirring and mixing module uses a bubble stirrer to ensure uniform distribution of photosynthetic organisms in the culture solution; The biosafety module manages and controls the nutrient solution in detail to remove impurities and harmful substances in the culture solution; The wastewater treatment module treats the wastewater generated during the bacterial and algal biological cultivation process.

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