609 results about "Drug tolerance" patented technology

The invention discloses an application of nanometer structure lipid carrier administration system in the antineoplastic drug to reverse the multiple-drug tolerance of tumour cell, which comprises the following parts: solid lipid material, liquid lipid material and antineoplastic drug, wherein the rate of the liquid lipid (such as oleic acid) is 0-30wt%; the solid lipid is selected from monoglyceride; the liquid lipid is oleic acid; the antineoplastic drug is Paclitaxel or adriablastina. The invention has highly effective cell uptaking and cytolymph condensing function with packing molecular target in the antineoplastic drug of cell, which avoids P-glucoprotein in the drug tolerant cytolymph from identifying the antineoplastic drug to reduce exclusion.

When multiple kinds of bacterial colonies are present in a petri dish and, for example, a drug tolerance is to be measured, harvesting of mixed colonies of different types of bacteria makes it impossible to accurately determine the drug tolerance. Also, it is required to improve the throughput of a device for harvesting a bacterial colony. From images illuminated from multiple directions, isolating bacterial colonies are automatically extracted. Next, the image feature amounts are calculated from the multiple images that are illuminated from multiple directions and colonies are grouped depending on the feature amounts. Then, bacterial colonies to be harvested are determined based on the results of the grouping.

The invention relates to a method for preparing silk fibroin film, especially a method for using covalence decoration to prepare novel antibiosis silk fibroin film. The inventive method comprises that removing foreign materials of Na2CO3 solution smelted silk fibroin, dissolving in lithium bromide water solution, drying, mixing alcohol with water, immerging in phosphate buffer solution, immerging in EDC HCl/NHS solution, immerging and washing in phosphate buffer solution, drying. The invention can adjust the density of silk fibroin, the density of antibiosis peptide solution and process covalence decoration, to control the antibiosis function. The invention has the structure and functions of natural animal silk fibroin, with surface antibiosis activity, long service life and non drug tolerance.

The invention relates to a pomegranate peel polyphenol antibiosis and antiphlogosis effervescent tablet as well as a preparation method and application thereof. The effervescent tablet is mainly prepared by raw material of pomegranate peel polyphenol, and auxiliary materials of sodium hydrogen carbonate, citric acid or tartaric acid, lactose, polyvinylpyrrolidone, magnesium stearate and superfine silica powder, has the functions of clearing heat, eliminating dampness, killing bugs and stopping itching, has strong suppression effect to women vagina pathogenic bacteria, features high safety, good stability, no obvious toxic or side effect, or drug tolerance, and provides convenience in using, carrying and storing for patients, is used for curing such diseases as vaginitis, vulvitis and the like caused by various trichomonad, epiphyte and bacteria, and can be used as preventability medicine for long-term use.

The invention discloses a plant disinfectant and a preparation method thereof. The plant disinfectant comprises the following raw materials: weeping forsythia, honeysuckle, liquorice root, golden thread, cortex phellodendri, raw rhubarb, safflower, flos caryophyllata, red paeony root and fineleaf schizonepeta herb. The preparation method comprises the following steps of pulverizing the raw materials, immersing the pulverized raw materials into distilled spirit, performing percolation, standing and filtering and adding peppermint oil and/or rose essential oil to obtain products of the plant disinfectant through subpackage. The plant disinfectant combines various plant raw materials with disinfection and sterilization, contains compound chemical components, can kill various bacteria and virus, solves the problems of the drug resistance, drug tolerance, dependence, accumulated toxicity and the like of the bacteria and the virus fundamentally, has no pollution to environment, and is an efficient disinfectant which has broad spectrum of sterilization and disinfection, safety, no toxic or side effect, quick response and stable properties.

This invention relates to a compound Chinese medicine micro powder and fish food for fish disease control, solving the problems of incapability of simultaneously controlling various fish diseases, dubious curative effect and easy generation of drug tolerance in current fish disease control. The compound Chinese medicine micro powder of the invention is obtained by carrying out ultramicro grinding on the following raw materials in parts by weight: 9-15 parts of folium isatidis, 6-9 parts of folium cortex eucommiae, 9-12 parts of ginkgo leaves, 3-9 parts of scutellaria baicalensis and 4.5-9.0 parts of radix sophorae flavescentis. The fish food for fish disease control of the invention contains 4%-6% of the compound Chinese medicine micro powder for fish disease control by weight percent. The compound Chinese medicine micro powder can control various fish diseases caused by various infectious agents, has confirmed curative effect, can improve fish immunity and promote growth and development, does not generate drug tolerance, and has no toxic or side effect. The fish food of the invention has good taste, thus fish schools like to eat the fish food.

The invention discloses an antibiotic and health care feed addictive and the method for preparing the same. The feed addictive is prepared with divalent nantokite, zinc solution and montmorillonite through absorption and ion exchange reaction, the copper content in montmorillonite is 0.5-5% by weight percent, and zinc is 1-10% by weight percent. The feed addictive can be used for poultry, aquatic creature, cow and sheep, and it possesses strong antibiotic action. It is hard for bacteria to generate drug tolerance. The product can effectively inhibit the propagation of enteric pathogenic bacteria, which is good for secretory of digestive enzyme and digestion of feed. The product can dramatically increase animal survival rate, reduce loose bowel movement and increase production capacity.

The invention, blonging to plant biotechnology field, relates a method of checking the virus of B hepatitis genovariation. The invention utilize the fluorescent light definite quantity PCR MGB Taqman probe technique, according to the YMDD nucleic acid sequence of B hepatitis virus, designing the genovariation of primer and probe checking HBV YMDD region. The invention adopts PCR reaction mixture A, B, Tap enzymes, HBV DNA extractant, and prepares diagnosis agent case after optimization and improvement with ddH20. The method can rapidly and exactly check the gene mutation situation of privileged site, quantitate the ratio of hybrid mutational virogene, quckly check the clinical hepatitis B drug therapia drug tolerance mutation. The method and agent case are not confined by medical equipment, so it is very easy to popularize.

The invention relates to a feed additive for promoting the growth of pigs and improving meat quality and a preparation method thereof, wherein the feed additive comprises the following components in percentage by weight: 15 to 30 percent of astragalus polyose, 5 to 10 percent of cassia seed, 10 to 30 percent of radix codonopsitis, 5 to 8 percent of atractylis ovata, 5 to 10 percent of liquorice, 2to 5 percent of garlicin, 7 to 12 percent of dried orange peel, 10 to 15 percent of tea, and 1 percent of vitamin. The feed additive mainly comprises Chinese medicinal herbal, tea and vitamin. The practice proves that: the additive containing the Chinese medicinal herbal, the tea and the like is used to substitute for antibiotics, which can improve the utilization rate of the feed, promote the growth of animals, prevent and cure diseases, and improve ecological environment. Immunologic active materials of the Chinese medicinal herbal in Chinese medical herbal preparations can strengthen the immune function of organisms, improve the anti-stress and anti-disease abilities of the animals, improve the production performance of the animals, have no (low) residue, and are difficult to produce the drug tolerance. By mixing and adding various components of the invention into the feed, the feed additive and the method can greatly improve the growth speed of the pigs, remarkably promote the meat quality and the yield, and have good economic value and social value.

The invention discloses a purely traditional Chinese agent and making method to prevent and treat chicken coccidiosis, which comprises the following parts: Chinese bulbul, dichroa febrifuga, sweet wormwood, hairy vein agrimony, feizi, areca seed, honeysuckle flower, astragalus root and so on. The making method comprises the following steps: weighing each drug material to add 1. 5 times water to soak; extracting 2h under 95 deg. c; filtering; condensing the filtrate under vacuum to form 1. 2 extract; adding 70% rice husk powder according to the weight of extract; drying. The invention doesn't produce drug tolerance without residuals and side effect to improve the utility of the fodder, which reduces the feeding cost of the chicken to improve the immunity of the chicken and disease resistance and growing speed.

The invention relates to a sterilization method capable of preventing a reverse osmosis membrane from being polluted and plugged by microbe. The sterilization method mainly comprises the steps of adding an oxidizing sterilizing agent NaClO (5%) solution to a raw water tank continuously; adding a reducing agent NaHSO3 (35% W/V) solution continuously after ultra-filtration; removing residual chlorine thoroughly; controlling a pH value of inlet water at a reverse osmosis inlet between 7.1 and 7.4; monitoring and controlling ORP before a security filter between 200 mv and 300 mv; and sterilizing by carrying out preventative addition of a non-oxidizing sterilizing agent DBNPA solution with a concentration of 50 ppm continuously for one hour once every week in the above flow according to the quick killing characteristic of the non-oxidizing sterilizing agent DBNPA. Meanwhile, NaClO addition to the raw water tank is stopped. The sterilization method mainly takes use of the oxidizing sterilizing agent accompanied by two non-oxidizing sterilizing agents to carry out preventative sterilization alternatively, controls drug tolerance and resistance of the microbe, can effectively prevent and control propagation and excessive multiplication of the microbe on the surface of the reverse osmosis membrane, thereby preventing the reverse osmosis membrane from being polluted and plugged by the microbe.

The invention discloses an acidic buffering sterilization gel for vagina gynaecologically. The gel consists of the following substances in parts by weight: 0.2-0.5 part of bactericide, 12-12.5 parts of glycerol, 83-84 parts of water, 1-1.5 parts of Carbomer, 1.5-2 parts of polycarbophil, 0.2-0.3 part of triethanolamine, 0.01-0.03 part of vitamin E, and 0.01-0.03 part of mint. The acidic bufferingsterilization gel has the advantages that: pathogenic bacteria can be quickly and effectively killed, inflammatory symptoms can be quickly and effectively relieved, normal pH value of the vagina is maintained, elasticity of the inner wall in the vagina can be kept, wetness of the vagina is maintained, and drug tolerance and drug resistance do not generate.

The invention belongs to the field of bio-medicine, and discloses a magnetic resonance imaging nano-carrier, a nano drug carrier system and a preparation method thereof. According to the invention, two targeting molecules, namely folic acid and cell-penetrating peptide, are adopted, so that a targeting effect on tumors is improved, and meanwhile, anti-tumor activity in vitro and in vivo is enhanced. PLGA, which is low in toxicity and good in biocompatibility, is taken as the anti-tumor drug carrier, and the terminal of the PLGA is modified with CS, so that the functional nano-system is prepared. Meanwhile, nuclear magnetic imaging drugs and anti-tumor drugs are effectively loaded, so that the anti-tumor drugs can reach a tumor focus part in a specific mode, and tumor region nuclear magnetic localization of ferroferric oxide nanoparticles can be implemented; and meanwhile, a therapeutic effect with high selectivity and low toxicity can be achieved. With the application of the invention,shortcomings of conventional cytotoxic drugs which are poor in selectivity, relatively strong in toxic and side effects, capable of causing drug tolerance easily and the like can be overcome; and a utilization degree of the anti-tumor drugs can be improved and toxic and side effects can be reduced. The preparation method of the drug carrier system is simple and easy to implement; and the preparedproduct (the drug carrier system) is good in repeatability and stability.

The invention discloses a core material of feed for a baby pig. The core material comprises the following substances in parts by weight: 3-18 parts of plant essential oil, 6-20 parts of complex multiple ore, 6-20 parts of active factor, 6-24 parts of skimmed milk powder, 2-12 parts of antibacterial peptide, 2-10 parts of an acidifier and 1-8 parts of an edulcorant. The core material of the feed for the baby pig, which is provided by the invention, does not contain any antibiotic drug, avoids problems of drug remaining and drug tolerance which are caused by an antibiotic, improves the pollution to an environment and soil, and improves the quality and the safety of meat; a ration feeding effect can also replace an antibiotic using effect; the demands of the normal growth and a nutrient substance of the baby pig are completely met.

The invention relates to a drinking type acidulant and a preparation method and application thereof. Each 100ml of the acidulant contains components of 15-25ml of formic acid, 25-35ml of lactic acid,15-25g of citric acid, 5-15g of fumaric acid, 1-8g of calcium formate, 1-8g of sodium butyrate and the balance deionized water, wherein all of the components are 100ml. The preparation method comprises the following steps of performing injection according to the amount of 50-80% of the total amount of water to a stirring tank, starting a power supply, performing stirring at 120r/min, slowly addingthe fumaric acid, then adding the calcium formate and the sodium butyrate, performing stirring for 15min, after the fumaric acid, the calcium formate and the sodium butyrate sufficiently dissolve inthe water, slowly adding the citric acid, the lactic acid and the formic acid separately, and finally, adding the remaining water for uniform stirring so as to obtain products. Compared with the priorart, the drinking type acidulant contains various organic acid and organic acid salts, can regulate the acid of a ration system, can reduce the pH value of the digestive tracts of animals, can improve the activity of digestive enzymes, can promote the digestion of nutrient substances, can effectively control diarrhoea of piggies at the weaning and child care period, can regulate the intestinal microecology balanced, and can promote growth of the piggies at the weaning and child care period. Besides, when the drinking type acidulant is drunk, growth and propagation of pathogenic microorganismsin animal organisms, feeds and environment can be greatly reduced, and the usage amount of antibiotics can be directly reduced, so that drug tolerance, remaining and pollution problems caused by antibiotics can be reduced.

The present invention relates to medicine technology and is especially new use of baicalein, which is one kind of effective components extracted from skullcap root and has been used in treating various kinds of inflammation, as antifungal medicine synergist. Baicalein is added into antifungal pyrrole medicine in the added amount of 4-16 mcg/ml. Experiments show that adding baicalein into antifungal medicine of fluconazole, ketocanazole, miconazole, etc can ensure the treating effect on fungal inflection and resist the drug tolerance of drug resisting fungi, so that baicalein may be used as the synergist of antifungal medicine.

The invention discloses a heterocyclic compounds capable of reversing drug resistance of tumor cells. Preparation and usage thereof are further disclosed. The inventive heterocyclic compound has a structure indicated by formula (I). Preparation includes steps of: preparing carboline carboxylic acid by conventional process; coupling secondary amino group of carboline carboxylic acid with L-amino acid benzyl esters to generate a N position protected carboline carboxylic -L-amino acid benzyl esters after protected by protecting group; condense with acetone in methanol under catalysis of triethylamine after detraction of protecting group. It is indicated in the in vitro test, the inventive heterocyclic compound can effectively reverse drug resistance of tumor cell towards anti-tumor drug, can be used as drug resistance inhibitor of tumor cell in clinic.

The invention discloses a preparation method of a taxol/DNA tetrahedral drug loading system and belongs to the field of biological drugs. The preparation method includes: mixing a taxol solution with a DNA tetrahedral solution, hatching, then centrifuging, and removing supernate to obtain the taxol/DNA tetrahedral drug loading system. The preparation method has the advantages that the water solubility of PTX is increased, the anaphylactic reaction of an existing PTX solvent which is the mixture of polyoxyethylated castor oil and anhydrous ethanol, lethal dose of 50% is lowered, the resource limitation problem is relieved effectively, the drug tolerance of the PTX can be reversed, and lethality to drug-tolerant tumors is increased.

The invention discloses a cholesterol-lowering ferment cultured milk and a preparation method thereof. The material milk is prepared by fermenting 3 mixing strains of the lactobacillus GL-03, bulgaria bacillus and streptococcus thermophilus, and a hawthorn extract; the technique comprises the following steps: the material milk is needed for milk-test and purifying, and then can be prepared for use, sucrose and a stabilizing agent are dissolved by little solution, and then added in the milk liquid after purifying; the temperature is enhancedto 60 DEG C for carrying out homogenate, sterilization, the temperature is lowered down to 43 DEG C, inoculation is carried out in a greenhouse of 40-45 DEG C for fermenting 4-5 hours, the temperature is cooled to under 10 DEG C after the pH value is 4.6-4.8, then the ferment cultured milk is done after being replaced to a refrigeratory of 0-4 DEG C for refrigeration. Shown by GL-03 culture in vitro test, the ferment cultured milk has strong acid and cholate resistant ability, good adhesive, obvious inhibit to common pathogenic entero becteria, without drug tolerance factors, is a lactobacillus applicable to permanent planting of human body intestinal tract, and simultaneously the deterioration ratio of the cholesterol in vitro can be up to 56 percent; proved by the animal-test, the cholesterol-lowering cultured milk has the function of lowering serum cholesterol.

The invention discloses a ginseng candy, which is prepared from the following components in parts by weight: 25-30 parts of cynomorium songaricum, 10-20 parts of ginsenoside, 5-10 parts of protein hydrolysate, 1-5 parts of enzyme, and 60-75 parts of malt. According to the ginseng candy, pure natural healthy foods are adopted as raw materials, the ginseng candy does not have toxic or side effect, can be used for supplying cell nutrition, inhibiting free radicals, improving body fatigue and accelerating blood circulation, and make people energetic. The ginseng candy has the characteristics of good anti-fatigue effect, quick response, no drug tolerance and wide applicable people.