Halophilic and basophilic sulfur oxidation complex microbial inoculant as well as preparation method and application thereof in biological desulfurization

A sulfur-oxidizing compound bacterial agent and basophilic technology, which is applied in the field of environmental biology to achieve high sulfur productivity, fast sulfur oxidation rate and bacterial growth rate, and high sulfide tolerance concentration

Active Publication Date: 2020-11-27
INST OF PROCESS ENG CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, it is difficult for a single sulfur-oxidizing bacteria to meet the above requirements at the same time. Therefore, the use of a composite bacterial group composed of a variety of sulfur-oxidizing bacteria can well meet the needs of industrial desulfurization, further expand the application range of biological desulfurization technology, and enhance the ability to deal with complex working conditions. ability, improve system stability, and strengthen desulfurization effect

Method used

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  • Halophilic and basophilic sulfur oxidation complex microbial inoculant as well as preparation method and application thereof in biological desulfurization
  • Halophilic and basophilic sulfur oxidation complex microbial inoculant as well as preparation method and application thereof in biological desulfurization
  • Halophilic and basophilic sulfur oxidation complex microbial inoculant as well as preparation method and application thereof in biological desulfurization

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0075] This embodiment provides a kind of halophilic alkaline-philic sulfur-oxidizing compound bacterial agent of the present invention, the operation is as follows:

[0076] (1) Take sulfalkalivibrio D301, thiomicrospiral BDL05, and thioalkalibacterium BDH06 preserved at 4°C, and activate them with seed liquid medium;

[0077] (2) When the OD of sulfoalkalivibrio D301, thiomicrospiral BDL05, and sulfoalkalibacterium BDH06 culture solution reached 5, the generated sulfur particles and bacteria were collected by centrifugation, respectively, to prepare sulfoalkalivibrio strains, sulfur Microspirillum species and Thioalkali species;

[0078] (3) Add 0.4g / L of sulfalkalivibrio bacteria, 0.3g / L of thiomicrospiral bacteria and 0.2g / L of thioalkalibacter bacteria into the compound flora culture medium, shake culture, and keep the temperature at 30°C;

[0079] (4) When the OD of the culture solution reaches 9, the culture is stopped, the culture solution naturally settles for 1 hou...

Embodiment 2

[0083] This embodiment provides a kind of halophilic alkaline-philic sulfur-oxidizing compound bacterial agent of the present invention, the operation is as follows:

[0084] (1) Take sulfalkalivibrio D301, thiomicrospiral BDL03, and thioalkalibacterium BDH06 preserved at 4°C, and activate them with seed liquid medium;

[0085] (2) When the OD of sulfoalkalivibrio D301, thiomicrospiral BDL03 and sulfoalkalibacterium BDH06 culture solution reaches 6, the generated sulfur particles and bacteria are collected by centrifugation respectively to prepare sulfoalkalivibrio strains, sulfur Microspirillum species and Thioalkali species;

[0086] (3) Add 0.3g / L of sulfalkalivibrio bacterial classification, 0.45g / L of thiomicrospiral bacterial classification and 0.15g / L of sulfuralkalibacterial bacterial classification into the compound flora culture medium, vibrate culture, and keep the temperature at 30°C;

[0087] (4) When the OD of the culture solution reaches 10, the culture is sto...

Embodiment 3

[0091] This embodiment provides a kind of halophilic-alkaline-sulfur-oxidizing composite bacterial agent of the present invention, its operation refers to the method in Example 1, the difference is only in the final obtained halophilic-alkaline-sulfur-oxidizing composite bacterial agent, in total The mass is 100%, including 70% of thioalkali bacteria D301, 20% of thiomicrospiral BDL05 and 10% of thioalkali bacteria BDH06.

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Abstract

The invention relates to a halophilic and basophilic sulfur oxidation complex microbial inoculant as well as a preparation method and application thereof in biological desulfurization. The halophilicand basophilic sulfur oxidation complex microbial inoculant comprises vibrio thioalkaligenes, thiobacillus thiospirillum and thiobacillus thiooxidans. The halophilic and basophilic sulfur oxidation complex microbial inoculant has the capacity of oxidizing sulfide to generate elemental sulfur, the yield of the elemental sulfur can reach 85% or above, and the removal rate of the sulfide reaches 99%or above; and the halophilic and basophilic sulfur oxidation complex microbial inoculant can better meet the requirements of industrial desulfurization, the indexes such as the elemental sulfur generation rate and the sulfide removal rate of the halophilic and basophilic sulfur oxidation complex microbial inoculant reach or exceed those of a single strain, the application range of a biological desulfurization technology can be expanded, the capacity of coping with complex working conditions is enhanced, the system stability is improved, and the desulfurization effect is enhanced.

Description

technical field [0001] The invention belongs to the field of environmental biology technology, and relates to a compound bacterial agent and its preparation method and its application in biological desulfurization, in particular to a halophilic and alkaline sulfur-oxidizing compound bacterial agent, its preparation method and its application in biological desulfurization. application. Background technique [0002] Biological desulfurization technology can oxidize sulfide in gas or water phase to generate elemental sulfur at normal temperature and pressure, and discharge it from the system. Compared with traditional physical and chemical methods, biological desulfurization technology has the advantages of mild conditions, no secondary pollution, and no system blockage. It has been applied to desulfurization projects such as wastewater and waste gas. The desulfurization bacteria used in this technology are mainly chemoautotrophic microorganisms that can utilize CO 2 as a car...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20B01D53/52B01D53/78B01D53/84C02F3/34C12R1/01C02F101/10C02F103/02C02F103/24C02F103/36
CPCC12N1/20B01D53/52B01D53/78B01D53/84C02F3/34C02F2101/101C02F2103/026C02F2103/24C02F2103/365Y02P20/59
Inventor 杨茂华邢建民穆廷桢苗得露
Owner INST OF PROCESS ENG CHINESE ACAD OF SCI
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