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Application of Stenotrophomonas rhizophila DSM14405 in reduction of hexavalent chromium

A technology of DSM14405 and Stenotrophomonas, which is applied in the direction of bacteria, microorganism-based methods, biological water/sewage treatment, etc., can solve the problems of incomplete treatment, secondary pollution, etc., to reduce content, reduce absorption, reduce toxic effect

Active Publication Date: 2018-07-06
RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In the treatment of chromium pollution, both chemical and physical methods may cause incomplete treatment or the possibility of secondary pollution

Method used

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  • Application of Stenotrophomonas rhizophila DSM14405 in reduction of hexavalent chromium
  • Application of Stenotrophomonas rhizophila DSM14405 in reduction of hexavalent chromium
  • Application of Stenotrophomonas rhizophila DSM14405 in reduction of hexavalent chromium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1: Discovery of Hexavalent Chromium Reducing Properties of Stenotrophomonas rhizophila DSM14405

[0032] (1) Source of the strain: Stenotrophomonas rhizophila DSM14405 was purchased from the American Type Microorganism Collection, and the deposit number is ATCC BAA-473 T .

[0033] (2) culture medium

[0034] Tryptone Soy Broth (TSB): tryptone 17g / L, soybean peptone 3g / L, glucose 2.5g / L, sodium chloride 5.0g / L, dipotassium hydrogen phosphate 2.5g / L, pH7.3± 0.2.

[0035] Tryptone Soy Agar Medium (TSA): tryptone 17g / L, soybean peptone 3g / L, glucose 2.5g / L, sodium chloride 5.0g / L, dipotassium hydrogen phosphate 2.5g / L, agar 15g / L, pH7.3±0.2.

[0036] Culture medium containing different concentrations of Cr(VI): 5000 mg / L Cr(VI) was used as the mother solution, and corresponding amount of mother solution was added to TSB medium to prepare Cr(VI) medium with different final concentrations.

[0037] (3) Whether Stenotrophomonas rhizophila DSM14405 has the ability...

Embodiment 2

[0043] Example 2: Determination of the Optimum Hexavalent Chromium Reduction Concentration of Stenotrophomonas Rhizophilus DSM14405

[0044] According to Example 1, it can be confirmed that Stenotrophomonas rhizophila DSM14405 has a better growth and reduction effect on Cr(VI) concentration of 100 mg / L or below. In this example, TSB medium containing 0 mg / L, 50 mg / L, 100 mg / L, and 200 mg / L of Cr(VI) were prepared, and the seed liquid of Stenotrophomonas rhizophilus DSM14405 was inoculated in the TSB In the culture medium, the optimal reduction concentration of hexavalent chromium of Stenotrophomonas rhizophila DSM14405 was determined according to the growth and reduction effect of Stenotrophomonas rhizophila DSM14405 in the above-mentioned TSB medium. Specific steps are as follows:

[0045] ①Preparation of seed solution of Stenotrophomonas rhizophila DSM14405

[0046] Take the bacterial solution that has been cultivated for 24 hours, inoculate it into the TSB culture solutio...

Embodiment 3

[0050] Example 3: Effects of Environmental Conditions on the Hexavalent Chromium Reducing Ability of Stenotrophomonas rhizophilus DSM14405

[0051] From embodiment 2, when known Cr (Ⅵ) concentration 50mg / L, the growth of Stenotrophomonas rhizophilus DSM14405 and hexavalent chromium reduction effect are better, so when this embodiment is doing culture condition optimization, use Cr ( Ⅵ) The concentration is 50mg / L as the research concentration.

[0052] (1) The influence of the initial pH value

[0053] When the Cr(Ⅵ) concentration is 50 mg / L, the temperature is 30°C, and the rotation speed is 180 rpm / min, the pH of the TSB medium is adjusted to five levels of 6.0, 6.5, 7.0, 7.5, and 8.0. The seed liquid with OD600 value of 1.0 was added to the culture medium in an amount of 1%, cultivated for 36 hours, and the OD600 value and Cr(VI) concentration were measured every 12 hours. The effect of initial pH value on the growth of Stenotrophomonas rhizophilus DSM14405 was as follows...

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PUM

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Abstract

The invention discloses application of Stenotrophomonas rhizophila DSM14405 in reduction of hexavalent chromium. The Stenotrophomonas rhizophila DSM14405 survives in an alkaline environment for reducing the hexavalent chromium to trivalent chromium, and the trivalent chromium is bonded with hydroxide to produce a chromium hydroxide precipitate. The Stenotrophomonas rhizophila DSM14405 has a fasterreduction rate of the hexavalent chromium, the reduction rate of the hexavalent chromium of 50 mg / L is up to 100% within 32 h, and the concentration tolerance to the hexavalent chromium is high. Whenthe concentration of the hexavalent chromium reaches 200 mg / L, thallus can also grow, and the reduction rate reaches 47.6% within 48 hours.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to the application of Stenotrophomonas rhizophila DSM14405 in reducing hexavalent chromium. Background technique [0002] Chromium salts are mainly used in metal processing, tanning and electroplating industries. In industrial production, in order to prevent corrosion of various equipment by circulating water, chromate is often added. In recent years, with the rapid development of my country's economy, more and more chromium salts are used in industrial production, and its production waste residue is currently one of the most important sources of heavy metal industrial pollution in the world. Therefore, the chromium pollution in industrial wastewater and waste gas is becoming more and more serious. There are mainly two forms of chromium in the environment: trivalent chromium and hexavalent chromium, and the chemical behavior and toxicity of the two forms are different. Among them, tr...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C12R1/01C02F101/22
CPCC02F3/34C02F2101/22C12N1/20
Inventor 庄绪亮吴诗敏高婕刘颖庄国强
Owner RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
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