Radiation-resistant penicillium sp. and application to biological treatment of absorbing radioactive strontium 90

A biological treatment and radiation-resistant technology, applied in the direction of radioactive purification, microorganism-based methods, microorganisms, etc., can solve the problem of limited ability to repair radioactively polluted environments

Active Publication Date: 2014-11-19
THE INST OF MICROBIOLOGY XINJIANG ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since most bacteria are sensitive to radioactivity, their ability to remediate radioactively contaminated environments is limited
So far, there is no report on the adsorption of radionuclides by radiotolerant fungi

Method used

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  • Radiation-resistant penicillium sp. and application to biological treatment of absorbing radioactive strontium 90
  • Radiation-resistant penicillium sp. and application to biological treatment of absorbing radioactive strontium 90
  • Radiation-resistant penicillium sp. and application to biological treatment of absorbing radioactive strontium 90

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Embodiment one: Radiation-resistant Penicillium ( Penicillium sp. ) Isolation and screening of F161 CGMCC NO.8381

[0035] 1. Separation: Penicillium radiata used in the present invention ( Penicillium sp. ) was sampled and isolated from a radiation-contaminated environment in a certain place in Xinjiang by the Institute of Microbiology Application of the Xinjiang Academy of Agricultural Sciences. A batch of well-growing microbial strains were screened out using different culture temperatures, pH values, and culture media as enrichment conditions, and one of them was selected The strain whose strain number is F161.

[0036] Separation steps: According to the gradient dilution method, weigh 10g of soil samples in 90mL sterile physiological saline, and perform gradient dilution after activation at 30°C for 30min. Select 10 -2 、10 -3 、10 -4 The dilutions were spread on the plates of PDA medium respectively, and each treatment was replicated three times, and culture...

Embodiment 2

[0043] Embodiment two: Radiation resistant penicillium ( Penicillium sp. ) Sequence determination and phylogenetic analysis of F161 CGMCC NO.8381

[0044] 1. DNA extraction: the fungal extraction method is as follows:

[0045] (1) 200 mg of bacterial cells were ground with liquid nitrogen, and 3 ml of 3% CTAB extraction buffer was added; placed in a water bath at 65°C for 45 minutes, and centrifuged at 4,000 r / min for 20 minutes at 4°C.

[0046] (2) Transfer the supernatant to a centrifuge tube, add 4 μl of 10 mg / ml protease, incubate at 37°C for 1 h.

[0047] (3) Add 800 μl Tris saturated phenol, shake well, and centrifuge at 13000 r / min for 10 min; take the supernatant.

[0048] (4) Add an equal volume of chloroform / isoamyl alcohol, shake well, and centrifuge at 13000 r / min for 10 min; take the supernatant.

[0049] (5) Add 10 mg / ml RNase, and treat overnight in a water bath at 37°C.

[0050] (6) Add 800 μl of chloroform / isoamyl alcohol, shake well, and centrifuge at 13...

Embodiment 3

[0060] Embodiment three: Penicillium radiata ( Penicillium sp. ) Radiation resistance of F161

[0061] Two conditions must be met for microorganisms to treat radioactive pollution in the environment, that is, they can survive in a radiation environment and have high adsorption selectivity for polluting nuclides. Penicillium F161 used was extracted from radionuclide-contaminated soil. 60 Co radioactive source γ-ray irradiation, the cumulative radiation dose is 10 kGy. By comparing the culture of irradiated samples and non-irradiated samples, it was found that its growth was not affected by irradiation, that is, Penicillium F161 has a strong ability to resist radiation. The radiation properties (gamma radiation) of Penicillium F161 were verified by the method established by Ferreira et al. Bacteria were cultured in PDA liquid medium to the stable stage, centrifuged at 4°C, washed with normal saline, and the suspension concentration in normal saline was controlled at 1×10 ...

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Abstract

The invention discloses radiation-resistant penicillium sp. and application to the biological treatment of absorbing radioactive strontium 90. Penicillium sp. F161CGMCCNo.8381 is obtained by sampling, screening, separation, screening and physiological and biochemical identification. When being applied to the biological treatment of absorbing a radioactive substance, the Penicillium sp. F161CGMCCNo.8381 is tolerant to 16 heavy metals such as Pb<2+>, Zn<2+>, Ni<+>, Co<2+>, Cr<2+> and Hg<2+>, and the tolerated concentration can reach 200 mg / L; remarkable and obvious technical effects are achieved particularly when the Penicillium sp. F161CGMCCNo.8381 is applied to the absorption of the radionuclide strontium 90, so that the Penicillium sp. F161CGMCCNo.8381 is proven to have a broad application prospect in the biological treatment of absorbing strontium.

Description

technical field [0001] The invention relates to a microbial strain and its application in the technical field of bioabsorption of radionuclides. Specifically, the invention relates to a radioresistant Penicillium radionuclide and its application in the technical field of radionuclide adsorption. Background technique [0002] Strontium 90 ( 90 Sr) is a radioactive isotope of strontium, one of the fission products of uranium, with a half-life of 28.8 years. Usually from nuclear explosions or nuclear fuel products. Strontium-90 can be used as a radioactive source of β rays, which is quite harmful to the human body. It is produced from uranium in nuclear tests and is inhaled by the human body in the form of dust, causing radioactive damage to the human body. [0003] Radioactive pollution is an important cause of human cancer, reproductive lesions, genetic disorders, senile dementia, and amnesia. High-dose radiation (>6.5Gy) directly leads to death; low-dose radiation (<...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14G21F9/12C12R1/80
Inventor 谢玉清王玮张志东张丽娟张瑞荣顾美英王钰赖成霞
Owner THE INST OF MICROBIOLOGY XINJIANG ACADEMY OF AGRI SCI
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