75 results about "Eupenicillium molle" patented technology

The invention discloses a genetic transformation method of PEG (Polyethylene Glycol) mediated penicillium sclerotiorum strain protoplast and application of the genetic transformation method. The method comprises the following steps: (1) culturing penicillium sclerotiorum to produce spores, germinating hyphae, and collecting and cleaning fresh penicillium sclerotiorum hyphae; (2) compounding Lysing Enzymes, Yatase, cellulase and pectinase to prepare a protoplast enzymatic hydrolysate, and then adding the protoplast enzymatic hydrolysate into the penicillium sclerotiorum hypha for enzymolysis to obtain a penicillium sclerotiorum protoplast; and (3) adding plasmids to be transformed into the penicillium sclerotiorum protoplast, and carrying out PEG (Polyethylene Glycol) mediated transformation and resistance screening to obtain a penicillium sclerotiorum protoplast transformant. According to the method, the number and regeneration rate of the protoplast can be remarkably increased, and the enzymolysis time can be effectively shortened.

The invention relates to the technical field of microorganisms, in particular to penicillium LDFST10 and application of the penicillium LDFST10 in promoting plant growth or preventing and treating stem rot. The preservation number of the penicillium LDFST10 is CGMCC (China General Microbiological Culture Collection Center) No. 41960. The application comprises the step of applying the penicillium LDFST10 or the fermentation product thereof to rhizosphere soil of the plant so as to promote the growth of the plant and prevent and treat basal stem rot. The invention researches and screens out the penicillium frutescens LDFST10 capable of promoting plant growth and inhibiting various pathogenic bacteria of stem rot, various growth promoting preparations or bacteriostatic agents or drugs aiming at the stem rot can be developed on the basis of the penicillium frutescens, and the penicillium frutescens LDFST10 has important value in the planting field of various crops such as corn, wheat and the like.

The invention belongs to the field of microorganisms, and particularly provides a non-pathogenic penicillium ochrochloron strain which is derived from garlic rhizosphere soil, is classified and identified as penicillium ochrochloron, is preserved in the China General Microbiological Culture Collection Center (CGMCC), and has the preservation number of CGMCC 41151. The strain has broad-spectrum bacteriostatic activity and can antagonize various plant pathogens; the plant growth promoting agent has the growth promoting characteristic, can secrete siderophores and phospholytic enzymes, and improves plant iron and phosphorus nutrition; the composition is safe and free of pathogenicity to crops. Tests show that the P4 strain can relieve plant iron deficiency yellowing and promote crop growth. The invention also provides application of the strain in biological control of plant diseases and plant growth promotion.

The present invention generally relates to an isolated Penicillium strain, named Penicillium olsonii TLL1, with a preservation number of NRRL 68252, and to the use thereof in promoting plant growth, and to the use of the Penicillium strain, named Penicillium olsonii TLL1, named Penicillium olsonii TLL1, named Penicillium olsonii TLL1, named Penicillium olsonii TLL1. Specifically, the present invention provides compositions suitable for promoting plant growth comprising the isolated fungal strains that can mitigate Al toxicity 5 and increase phosphate effectiveness, as well as methods of their promotion of plant growth.

The invention discloses a microbial agent for increasing manure compost temperature and application thereof in high-temperature compost, and belongs to the field of agricultural solid waste treatment. The microbial agent for increasing the manure composting temperature comprises phanerochaete chrysosporium, bacillus stearothermophilus, penicillium oxalicum, streptomyces, common thermoactinomycetes and bacillus subtilis. The livestock padding manure high-temperature clean composting technology is established through adjustment and optimization, the purposes that the temperature of the padding manure is increased fast, the high-temperature period is long, the composting period is shortened, base materials are fully degraded are achieved, reference can be provided for resource utilization of livestock and poultry breeding solid waste, and important application value is achieved.

The invention belongs to the technical field of microorganisms, and particularly relates to bacillus velezensis A-39 and application of the bacillus velezensis A-39 in prevention and treatment of postharvest diseases of citrus, and the preservation number of the bacillus velezensis A-39 is CCTCC NO: M 20252917. The bacillus velezensis A-39 generates soluble antibacterial substances, does not generate volatile antibacterial substances, and has good prevention and treatment effects on citrus green mold, citrus anthracnose and citrus black rot. The result of the embodiment shows that the strain A-39, the bacterial liquid, the fermentation liquid and the metabolite of the strain A-39 can obviously inhibit the mycelial growth of penicillium digitatum; the control effect of the strain A-39 on the penicillium citrinum induced by penicillium digitatum is 100%; under natural conditions, the morbidity of citrus anthracnose treated by the strain A-39 is 0.33%, and the morbidity of citrus black rot is 0.33%; the control effect of the strain A-39 after fruit picking under the bacterial liquid concentration of OD600 = 0.3 is 68.84%.

The present invention relates to the technical field of microbial pharmaceutical chemistry, specifically to a method for preparing a Penicillium fungus ( Penicillium daleae The present invention relates to the medical use of a polyketide compound with a unique cage-like structure isolated from the fermentation product of a strain of the bacterium Mycobacterium spp. Using patch-clamp techniques to measure the action potential discharge frequency and spontaneous excitatory post-synaptic currents (sEPSCs) of hippocampal basket cells, the present invention confirmed that the compound has significant inhibitory activity on neuronal excitability and is non-toxic to N2a neurons at active concentrations. The compound can be used to prepare new drugs for treating diseases associated with neuronal hyperexcitability.

The invention belongs to the technical field of biological prevention and control of microorganisms and plant diseases, and particularly relates to a Penicillium griseofulvum strain antagonizing soft rot of Pinellia ternate and application of the Penicillium griseofulvum strain in prevention and control of plant diseases. The Penicillium griseofulvum AFP-25 strain provided by the invention is a strain of pinellia ternata soil rhizosphere microorganism, is safe to human, livestock and crops, and is friendly to the environment. The Penicillium griseofulvum has a very strong antagonistic effect on soft rot of pinellia ternate, is easy to colonize in pinellia ternate plants and surroundings thereof, utilizes Penicillium griseofulvum to biocontrol soft rot of pinellia ternate, is a stable Penicillium griseofulvum biocontrol mode, is low in cost, and can better solve the problem that large-area outbreak of soft rot of pinellia ternate cannot be controlled in agricultural production. Meanwhile, the penicillium griseofulvum can also effectively inhibit generation and spreading of other plant fungal diseases such as root rot, so that the penicillium griseofulvum has good practical application value.

The invention discloses a microbiological degraded Betallop simulation sample and a preparation method thereof, and belongs to the technical field of cultural relic degradation simulation. The method comprises the following steps: inoculating a specific decay-causing fungus suspension on the surface of a bay leaf simulated substrate for microbial degradation, wherein the specific decay-causing fungi comprise penicillium chrysogenum and humicola brownish black. The penicillium chrysogenum and the humicola brownish black obtained through screening have important values for researching a biological degradation mechanism of the cultural relics and developing a targeted biological prevention and control technology, and the simulation sample prepared by the method provides a standardized sample for protecting and repairing the Betashes; the method can be used for safely and efficiently verifying various reinforcing materials, mildew preventive, cleaning agent, protection process and repair technology and researching the aging mechanism, greatly reduces the risk of directly testing on precious cultural relics, and has important significance on promoting the scientific protection of plant fiber cultural relics such as Beijing.

The present application belongs to the technical field of postharvest disease control, and particularly relates to a biocontrol Serratia marcescens and its use for postharvest disease control and storage of pears. The steps are as follows: the Serratia marcescens is activated and cultured, and the bacterial cells are obtained by centrifugation after the culture; the bacterial cells are resuspended in sterile physiological saline to obtain a bacterial suspension; the bacterial suspension is injected into the holes punched on the surface of the pears, and then an equal volume of Penicillium expansum spore suspension is injected, so that the effective control of the Penicillium disease caused by Penicillium expansum is achieved; or the bacterial suspension of the Serratia marcescens is uniformly sprayed on the surface of the pears, and then naturally dried, so that the use for postharvest disease control and storage of the pears is achieved; the present application can significantly reduce the occurrence of the postharvest Penicillium disease of the pears, and reduce the natural rot rate of the pears after harvest, and is safe and environmentally friendly, and not only has no significant adverse effect on the main quality indicators of the postharvest pears, but also can maintain the storage quality of the pears, and achieves a significant technical effect, and has a wide application prospect.

This invention belongs to the field of microbial technology, specifically relating to an Kodamaea strain HBF12 and its applications. The Kodamaea strain HBF12 provided by this invention is deposited at the China Center for Type Culture Collection (CCTCC), with accession number CCTCC M 20241462. Verification through examples shows that this strain has significant inhibitory effects on *Geotrichum citri-aurantii* and *Penicillium digitatum*, and exhibits good preventative effects against postharvest diseases of citrus, particularly sour rot and green mold. Strain HBF12 demonstrates good biocontrol potential, providing a new resource for the safe control of postharvest diseases of citrus.

This invention discloses a compound fungal agent containing Penicillium citrinum and Trichoderma guizhouense, wherein the Penicillium citrinum is Penicillium citrinum (… Penicillium citrinum NJAU-T106, the Guizhou Trichoderma is Guizhou Trichoderma ( Trichoderma guizhouense NJAU4742. This invention also discloses its preparation method and its application in promoting banana plant growth, increasing banana yield, and controlling banana wilt disease. This invention screened Penicillium citrinum (NJAU4742) from banana disease-suppressing soil to obtain a strain with good biocontrol and growth-promoting effects. Penicillium citrinum The study used NJAU-T106 and innovatively combined it with Trichoderma guiyuanensis NJAU4742 to construct a synergistic system of Penicillium citrinum and Trichoderma guiyuanensis. This effectively reduced the incidence of banana wilt disease while better promoting banana plant growth and increasing banana yield, thereby driving the green and sustainable development of the banana industry.

The present application relates to the technical field of microbial inoculant, in particular to a compound microbial inoculant containing penicillium oxalicum and a preparation method thereof, the compound microbial inoculant containing penicillium oxalicum comprises the following raw materials in parts by weight: penicillium oxalicum solid-state fermentation product 3-5 parts, zeolite powder 20-25 parts, potassium fulvate 5-10 parts, humic acid 10-15 parts, sodium lignosulfonate 8-12 parts, and modified diatomite 30-40 parts. The compound microbial inoculant containing penicillium oxalicum prepared by the present application has excellent soil-borne disease control effect and good stability.

The present invention relates to the field of genetic engineering technology, and specifically provides a high-yield xylanase-producing Trichoderma reesei strain and its application. The applicant firstly isolates the strain from Penicillium decumbentum ( Penicillium reclining ) was overexpressed in a Trichoderma reesei host to construct a recombinant expression strain. This strain was then used as a starting strain for UV mutagenesis, resulting in a mutant strain that significantly increased xylanase expression. The mutant strain is deposited with CCTCC NO: M2021920. This mutant strain can be widely used in xylanase production, helping to reduce the production cost of the enzyme.

The present application relates to a kind of penicillium for plant disease biological control and its application.The present application is obtained by research and screening a new biocontrol agent Penicillium brefeldianum 54-13, the fermentation product thereof can effectively activate the immune induction level of plant, improve the disease resistance of plant caused by wheat powdery mildew, wheat scab and rice blast fungus, including wheat powdery mildew, wheat scab and rice blast fungus etc., provide a safe and environmentally friendly disease control strategy for the sustainable development of wheat and rice.

The invention provides a method for preparing DMOA mixed-source terpenoids from penicillium sclerotiorum HY5, the DMOA mixed-source terpenoids and application of the DMOA mixed-source terpenoids in herbicides, and belongs to the technical field of weed control. The penicillium sclerotiorum HY5 is utilized to successfully prepare the DMOA mixed-source terpenoid compound capable of regulating and controlling the growth of the weeds, the growth of the weeds can be promoted or inhibited, and the penicillium sclerotiorum HY5 has the development potential of serving as a green pesticide lead compound and can be used for developing natural product herbicides or plant growth regulator products with brand-new structures and unique action mechanisms.

The invention discloses a penicillium griseofulvum FZ61 strain and application thereof in prevention and treatment of panax notoginseng leaf diseases, and belongs to the technical field of microorganisms. The penicillium griseofulvum FZ61 strain (the preservation number is CGMCC (China General Microbiological Culture Collection Center) No.41462). Compared with penicillium griseofulvum strains FZ37 and FZ46 obtained through separation in the same batch, the penicillium griseofulvum strain FZ37 and FZ46 has excellent bacteriostasis, siderophore production, IAA production, cellulase production, nitrogen fixation and inorganic phosphorus dissolving capacities, easily and rapidly occupies a beneficial ecological niche at plant phyllosphere, can directly inhibit growth of main pathogenic bacteria of panax notoginseng leaves, can also improve the activity of related defensive enzymes of panax notoginseng leaves after foliage spraying, and has a wide application prospect. The leaf resistance is enhanced, the panax notoginseng black spot, the panax notoginseng round spot and the panax notoginseng anthracnose are prevented and treated, and the harm of leaf diseases to panax notoginseng planting is effectively reduced. In addition, the strain also has a remarkable inhibition effect on pathogenic bacteria of leaf diseases such as soybean blight, potato early blight and corn southern leaf blight which are seriously harmful in agricultural production, and can be used as a high-quality biocontrol bacterium resource for biological control of leaf diseases of crops.

This invention discloses a comprehensive control method for black rot disease in cauliflower containing a biological agent, comprising the following steps: S1, transplanting treatment: 200-500 ml of the mixed biological agent is applied to the roots of transplanted cauliflower seedlings as a root drench; the mixed biological agent contains at least 1 × 10⁻⁶ viable bacteria. 8 S1. A bacterial suspension of *Penicillium restrictum* and *Paecilomyces lilacinus* at a CFU / mL ratio of 1-3:1; S2. Spraying treatment: After transplanting cauliflower seedlings, spray the plants with a compound microbial agent; the compound microbial agent contains a bacterial suspension of *Penicillium restrictum* and *Paecilomyces lilacinus* at a volume ratio of 4-6:1; the viable count of both *Penicillium restrictum* and *Paecilomyces lilacinus* bacterial suspensions is not less than 1×10⁻⁶. 8 CFU / mL. The method described in this invention can effectively reduce the incidence of black rot in cauliflower during the transplanting to survival period, survival to heading period, and heading to harvest period.

The invention relates to an acid soil improvement fungicide based on penicillium photosporum and alkaline minerals and a preparation method and application thereof, the fungicide is composed of penicillium photosporum spore powder, the alkaline minerals and humic acid according to a certain mass ratio, and the alkaline minerals are formed by mixing calcium carbonate and magnesium silicate according to a specific ratio. The thermal setting formula and process can significantly improve the performance of the microbial inoculum, so that the colonization rate of spores in acid soil is greatly improved, and the pH buffer efficiency is also significantly improved. After the microbial agent is applied to acid soil, the pH value can be synergistically increased, the aggregate stability is enhanced, nutrients are activated, and the microbial agent is suitable for remediation of acid soil in farmlands, orchards and the like.

The invention provides Penicillium ochrochloron Ynauu-PO resistant to lead and cadmium and capable of antagonizing panax notoginseng root rot and application of the Penicillium ochrochloron Ynauu-PO, and relates to the field of microorganisms. The preservation number of the penicillium ochrochloron Ynauu-PO is CCTCC NO: M 20252802, the strain can have the capabilities of efficiently resisting lead and cadmium and antagonizing panax notoginseng root rot at the same time, and the technical bottlenecks that the survival rate of microorganisms in high-concentration lead and cadmium polluted soil is low and the remediation effect is poor are solved; an environment-friendly biological control scheme is provided, so that the use of chemical pesticides is reduced, and the environmental pollution and the drug resistance of pathogenic bacteria are avoided.

The invention discloses application of a compound microbial agent in prevention and treatment of leguminous crop root rot and crop growth promotion, and relates to the technical field of microorganisms, and the key point of the technical scheme is as follows: the compound microbial agent is a compound of a strain of Penicillium spinulosum LS001 and a strain of Trichoderma asperellum LS002. The strains are analyzed and identified to be P.spinulosum LS001 and T.asperillum LS002 based on a culture form and an ITS sequence, and plate antagonism proves that the two strains and sterile filtrate thereof have a very good inhibition effect on fusarium oxysporum; pot experiments prove that a single strain fermentation broth and a compound microbial agent have significant promotion effects on plant heights, root lengths and fresh weights of four selected crops; and after the root is irrigated with the complex microbial inoculant for 15 days, the control effect on the soybean root rot caused by fusarium oxysporum reaches 73.69%. The results show that the Penicillium spiniferum LS001 and the Trichoderma asperellum LS002 are disease-preventing and growth-promoting microbial strains with good effects, and the microbial agent formed by compounding the Penicillium spiniferum LS001 and the Trichoderma asperellum LS002 has efficient disease-preventing and growth-promoting effects on leguminous crops.

The invention discloses a growth conditioner for planting tricholoma matsutake and a preparation method thereof, and belongs to the technical field of tricholoma matsutake planting.The growth conditioner for planting tricholoma matsutake is prepared from, by weight, 5-8 parts of penicillium citrinum metabolite, 2 parts of arbuscular mycorrhizal fungi with the spore content being 20-30 / g, 5 parts of bacillus subtilis 108-1010 CFU / g and 10-12 parts of matrix. The pH value of the saline-alkali soil can be reduced, meanwhile, the organic matter content of the soil is increased, and the method is suitable for planting tricholoma matsutake; the beta-glucosidase activity of the tricholoma matsutake is promoted, the biomass of cell walls of pine trees serving as host trees is easily utilized, biological energy is obtained, and the growth of the tricholoma matsutake is promoted.

The invention provides a combined biological agent as well as a preparation method and application thereof, and relates to the technical field of soil remediation. The combined biological agent is prepared from a penicillium oxalicum fermentation solution, a chaetophyllum fermentum fermentation solution, a thiamine-solubilizing clostridium fermentum fermentation solution, a nitrogen-fixing functional bacterium fermentation solution, a phosphorus-solubilizing bacillus megaterium fermentation solution, a bacillus mucilaginosus fermentation solution and a pseudomonas aeruginosa fermentation solution. According to the combined biological agent, through the synergistic effect of multiple functional strains, the effective state concentration of heavy metal in soil is effectively reduced, meanwhile, the soil fertility is improved, the survival rate and colonization ability of the agent in an extreme environment are enhanced, the vegetation coverage rate is remarkably increased, and the soil quality is improved. And an efficient solution is provided for ecological restoration of the solid waste storage yard of the nonferrous metal mining area in the northwest arid region.

The invention discloses Penicillium carmichaeli and application thereof in prevention and control of crop stem rot, and belongs to the technical field of green prevention and control of crop diseases. According to the invention, a strain of Penicillium cameberti JK10 is separated from rhizosphere soil of crops, and the Penicillium cameberti JK10 has an obvious antagonistic effect on pathogenic bacteria, namely fusarium, of crop stem rot, has the properties of nitrogen fixation, potassium dissolution, phosphorus dissolution and siderophore production, and can be used as a novel green crop disease prevention and control preparation.

The present invention discloses a method for rapidly separating white rot fungi infected by miscellaneous fungi, belonging to the technical field of microbial culture. This method mainly includes four steps: spore liquid preparation, isolation and purification culture, identification culture, and scale-up culture. Different from traditional methods such as streak separation and addition of inhibitors, this method mainly conducts restrictive culture on common miscellaneous fungi such as Penicillium and Trichoderma in production applications through a white rot fungi selective medium, and rejuvenates the nutrition of the target strain. This method is simple to operate, has a high success rate, effectively reduces the time cost, and further improves the production efficiency. In addition, the white rot fungi separated and purified by this method have high activity and stable quality.

The invention provides disease-resistant and growth-promoting penicillium oxalicum AF-P1 and application thereof, and belongs to the technical field of microorganisms. The invention provides a penicillium oxalicum AF-P1, and the preservation number of the penicillium oxalicum AF-P1 is CGMCC (China General Microbiological Culture Collection Center) No.41803. The penicillium oxalicum The Penicillium oxalicum AF-P1 has the characteristics of high salt resistance and strong alkali resistance, has the capability of producing siderophores, has the capability of producing acid, has the capability of dissolving inorganic phosphate, can improve the stress resistance of plants, and the like. The Penicillium oxalicum AF-P1 can inhibit the growth of fusarium oxysporum, fusarium oxysporum, pythium miscanthus, Aparis minuta and phomopsis cantoniensis, so that the Penicillium oxalicum AF-P1 is beneficial to prevention and control of plant diseases caused by the pathogenic bacteria. The result of the embodiment shows that the Penicillium oxalicum AF-P1 can be used for preventing and treating rice seedling blight and tomato fusarium wilt.

The application discloses a black soldier fly breeding method for improving the biological conversion capacity of black soldier flies on agricultural wastes. Penicillium sclerotigenum The application discloses a black soldier fly breeding method for improving the biological conversion capacity of black soldier flies on agricultural wastes. The application discloses a black soldier fly breeding method for improving the biological conversion capacity of black soldier flies on agricultural wastes. The application discloses a black soldier fly breeding method for improving the biological conversion capacity of black soldier flies on agricultural wastes. The application discloses a black soldier fly breeding method for improving the biological conversion capacity of black soldier flies on agricultural wastes. The application discloses a black soldier fly breeding method for improving the biological conversion capacity of black soldier flies on agricultural wastes. The application discloses a black soldier fly breeding method for improving the biological conversion capacity of black soldier flies on agricultural wastes. The application discloses a black soldier fly breeding method for improving the biological conversion capacity of black soldier flies on agricultural wastes. The application discloses a black soldier fly breeding method for improving the biological conversion capacity of black soldier flies on agricultural wastes. The application discloses a black soldier fly breeding method for improving the biological conversion capacity of black soldier flies on agricultural wastes.

The invention provides a biocontrol penicillium and application thereof, the biocontrol penicillium is Penicillium oxalicum POIL-1, the biocontrol penicillium is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.42328. The biocontrol penicillium has the advantages that the biocontrol penicillium is the Penicillium oxalicum POIL-1, and the biocontrol penicillium is the Penicillium oxalicum POIL-1; it is found for the first time that the strain or culture filtrate of the strain can effectively inhibit various common potato pathogenic bacteria including phytophthora infestans, can be used for field biological control of major diseases such as potato late blight and the like, and is beneficial to reduction of use of chemical pesticides. In addition, the strain significantly promotes the growth of solanaceae crops such as potatoes, tobaccos and tomatoes, has good phosphate solubilizing ability, and is helpful for improving the crop yield and reducing the use of phosphate fertilizer. The Penicillium oxalicum POIL-1 strain can be used for biological pesticide development, has triple effects of preventing diseases, promoting growth and improving soil quality, is green and environment-friendly, is low in cost, and has good application potential.

The application discloses a kind of livestock and poultry manure high-temperature rapid composting compound microbial inoculum and preparation and application thereof, the microbial inoculum is prepared by the fermentation product of Brevibacillus borsalensis and the fermentation product of Penicillium chrysogenum mixture.The microbial inoculum can promote rapid composting temperature rise, shorten composting time, while significantly reducing ammonia and nitric oxide emissions during composting, increase the content of nitrogen, phosphorus and potassium in composting, and improve composting quality.It is suitable for industrial scale production requirements, and is easy to use in large areas.

The application belongs to the technical field of microorganisms, and particularly relates to a salt stress-resistant and phosphorus-solubilizing Penicillium oxalicum as well as products and applications thereof. The application provides the Penicillium oxalicum, which is preserved on October 26, 2022, preserved in the China General Microbiological Culture Collection Center, and has a preservation number of CGMCC No. 40346. The Penicillium oxalicum provided by the application has the functions of salt stress resistance and phosphorus solubilization, can improve the utilization rate of soil insoluble phosphate in a saline-alkali soil, significantly improves the content of soluble phosphorus in the soil, has a wide application prospect in soil remediation and soil fertility improvement in the saline-alkali soil, and has far-reaching significance in agricultural production activities.