165 results about "Eupenicillium molle" patented technology

The present invention relates to a method of enhancing growth conditions for plants by growing the plants in soil containing, in proximity to the plant roots, both a phosphorus source and at least two strains of the fungus Penicillium, particularly P. bilaiae, more particularly strains NRRL 50162 and NRRL 50169.

The invention provides a penicilliums p. strain and application thereof. Eucalyptus endophyte is a penicilliums p. strain 1, and the collection number is CGMCC No.4297. The eucalyptus endophyte inoculated to eucalyptuses can improve the low phosphorus stress resistance of the eucalyptuses and promote phosphorus absorption of the eucalyptuses. Because the eucalyptus endophyte is used for promoting the phosphorus absorption of the eucalyptuses, and when the strains are inoculated to the eucalyptus plants, the activities of the plant phosphatase and the soil phosphatase can be improved, the pH value of the soil rhizosphere can be reduced, and the plant P utilization rate is greatly improved; therefore, the purpose of promoting normal growth of the plants in the low phosphorus soil is fulfilled, and the utilization rate of the soil phosphate fertilizer is greatly reduced.

The invention discloses a penicilliumgriseofulvum XJ-EP-058 strain as well as a microbial preparation resisting banana vascular wilt and application thereof, belonging to the technical field of microbial pesticides. The strain is penicilliumgriseofulvum XJ-EP-058 with the preservation number of CGMCC (China General Microbiological Culture Collection Center) No.5467. The microbial preparation, containing the strain, for resisting the banana vascular wilt is obtained by test tube seed culture, liquid seed culture for the strain and liquid fermentation culture for the strain. The strain and the microbial preparation, containing the strain, for resisting the banana vascular wilt have obvious in-vitro and in-vivo preventing effects and have the germination inhibition rate reaching 100% to spores of banana vascular wilt bacteria, and the average diameter of bacteriostatic zones to the banana vascular wilt bacteria is 14.5mm; and a result of a greenhouse potted living organism test shows that the strain and the microbial preparation have the average control effect reaching 74.38% on the banana vascular wilt bacteria. The microbial preparation has the advantages of low raw material cost and simple and feasible production process.

The invention discloses a penicillium vulpinum fungi strain and a method for preparing levo 7-hydroxyl butylphthalide ((S)-(-)-butyl-7-hydroxyl phthalide) employing the fungi strain. The fungi strain disclosed by the invention is a penicillium vulpinum fungi strain NCC3421 (penicillium vulpinum) and is preserved at the China General Microbiological Culture Collection Center (CGMCC for short); and the preservation number is CGMCC No.9094. Under the culture condition provided by the method, the fermentation unit of the levo 7-hydroxyl butylphthalide reaches over 1600mg/L; the fermented product is relatively single in components, and easy to separate and purify, and can be applied to industrialized production of the levo 7-hydroxyl butylphthalide.

The invention discloses a high-activity composite cellulase and preparation thereof, and an application method for the same in enzymatic saccharification of wood fiber. According to the invention, Eupenicillium javanicum ZN-205 is subjected to shake-flask fermentation so as to produce beta-glucosidase; best enzyme production conditions are that an initial pH value is 6, the concentration of peptone is 0.75%, the concentration of microcrystalline cellulose is 2.5%, the addition amount of tween-80 is 0.05%, culture temperature is 28 DEG C, the volume of liquid filled in a 250 ml triangular flask is 100 ml, a revolving speed of a shaker is 175 r/min, inoculum amount is 5% and the highest activity of beta-glucosidase is 2.312 IU/ml; beta-glucosidase prepared from Eupenicillium javanicum ZN-205 and cellulase prepared from Trichoderma reesei Rut C-30 are compounded, and an obtained optimal ratio of beta-glucosidase activity to filter paper activity of 1.4. Utilization of the compounded enzyme for enzymatic saccharification of wood fiber enables high-efficiency and low-cost enzymatic saccharification effects to be achieved.

The invention relates to marine streptomyces with antibacterial activity and belongs to the technical field of microbes. The marine streptomyces is streptomyces chumphonensis AM-4, is registered and collected in China General Microbiological Culture Collection Center on October 30, 2017 and has a collection number of CGMCC No.14843. The streptomyces chumphonensis AM-4 has an excellent inhibitory effect on ccolletotrichum glecosporioides, penicillium italicum, penicillium digitatum and pathogen of brown stem-end rot, and has the potential of being developed into a biopesticide.

The invention relates to a treatment method for dephosphorizing and denitrifying sewage. The method is characterized by carrying out treatment by combining a physical method and a biological agent, wherein the biological agent comprises the following raw materials in parts by weight: 5-7 parts of paracoccus denitrificans, 5-7 parts of saccharomyces cerevisiae, 4-5 parts of rhodococcus, 4-5 parts of bacillus amyloliquefaciens, 3-4 parts of clostridium papyrosolvens, 3-4 parts of penicillium ochrochloron, 2-3 parts of pseudomonas and 1-2 parts of bacillus cereus. The biological agent contains various microorganisms with excellent capacity of degrading refractory pollutants, is reasonable in compatibility of the strains, has good degrading effects and has a broad application prospect.

The invention discloses a marine fungi penicillium crustosum bacterial strain, quinolinone compounds derived from marine fungi penicillium crustosum, and a preparation method and applications of the quinolinone compounds. Specific structural formulas of the quinolinone compounds are represented by formula R1-formula R6. According to the preparation method, a culture medium is inoculated with the marine fungi penicillium crustosum bacterial strain AP2T1 CGMCC No.8516 for static fermentation; an obtained fermentation broth is extracted with ethyl acetate, and mycelium is extracted with an organic solvent; obtained crude extracts are mixed, an obtained mixture is subjected to sephadex column chromatography, reverse-phase silica-gel column chromatography, and thin layer chromatography detection using organic solvents; an obtained elution ingredient containing intermediates is subjected to methylation, and thin layer chromatography and reverse-phase silica-gel column chromatography separation and purification so as to obtain target compounds R1-R6. The target compounds R1-R6 possesses wide application prospect in preparation of antitumor drugs, antibacterial agents, and agricultural insecticides.

The invention discloses tephrosia purpurea endophytic fungus penicillium griseofulvum TPL25 and application of a fermentation product of the tephrosia purpurea endophytic fungus penicillium griseofulvum TPL25 in plant disease prevention fungal pathogens. The tephrosia purpurea endophytic fungus penicillium griseofulvum TPL25 is preserved in China Center for Type Culture Collection, and the preservation number is CCTCC NO: M2014607. Fungus fermentation liquor, fermentation liquor extractives and mycelium extractives have the strong mycelium grow inhibition function on sclerotinia rot of colza, tobacco blackleg and other phytopathogen, excellent strains are provided for development of microbial pesticide, and in addition, the tephrosia purpurea endophytic fungus is studied for the first time.

The invention discloses a gene highly expressing a nuclease P1 and an expression vector and a host cell comprising the gene and an application for the gene. The invention discloses a recombinant pichia yeast CGMCC No.3188 expressing the gene of nuclease P1 produced from Penicillium citrinum and its construction method. The inventive recombinant engineering strain pichia yeast CGMCC No.3188 can highly express Penicillium citrinum nucP gene. The secretion-expressed nuclease P1 has high phosphodiesterase activity, is used for hydrolyzing RNA, has high product yield and little generated impurity and reduces working procedure of downstream separation as well as decreases production cost. The invention uses the recombinant engineering strain pichia yeast CGMCC No.3188 to perform high density fermentation. The enzyme activity is 1.5 times by the wild strains. The acquired recombinase is applied to RNA solution enzymatic hydrolysis, thus, reaching the hydrolysis rate of more than 40% and the acquired hydrolysate by-product is substantially reduced.

The invention discloses a preparation method of penicillium oxalicum and application of penicillium oxalicum in phosphate solubilizing, growth promoting and fusarium graminearum antagonism, and belongs to the technical field of environmental biology. The penicillium oxalicum MEM02 can inhibit germination of fusarium graminearum spores in soil and remarkably reduce infection of the fusarium graminearum spores to wheat, and meanwhile the penicillium oxalicum MEM02 can efficiently solubilize phosphorus and promote plant growth. Therefore, the penicillium oxalicum MEM02 has good application prospects in the aspect of developing new biopesticides or new biological control microbial agents.

The invention discloses a Polygonum cuspidatum root endophytic strain. The strain is named Penicillium oxalicum G3 in a classified way, the strain was collected in China General Microbiological Culture Collection Center on January 17, 2011, and the collection number is CGMCC NO.4558. Polydatin in Polygonum cuspidatum can be transformed into resveratrol by inoculating the strain into a culture medium containing Polygonum cuspidatum boiling extracting solution, so that the polydatin can be directly transformed into the resveratrol through microbial fermentation, the extraction of the polydatin is not required, the cost can be saved, and the pollution to the environment can be reduced.

A methylotrophic bacillus strain (Bacillus methylotrophicus) for biocontrol and application thereof are disclosed. The bacterium Bacillus methylotrophicus BCN2 is deposited in the China General Microbiological Culture Collection Center under the accession number CGMCC No: 16443. The invention comprises isolation and culture of the strain and molecular identification of 16S rDNA; effective inhibiting effects of Methylotrophic Bacillus volatiles against common spoilage fungi (Fusarium oxysporum, Staphylococcus aureus, Trichoderma viride, Colletotrichum gloeosporioides, Penicillium expansum) ontwo-zone plate and loquat fruits and the antiseptic application of the volatile matter in loquat storage. The volatile antimicrobial substances produced by Bacillus methylotrophicus (BCN2) could not contact fruit directly, It can be used for preparing biocontrol fresh-keeping agent which can inhibit spoilage fungi such as Fusarium oxysporum, Staphylococcus aureus, Trichoderma viride, Colletotrichum gloeosporioides, Penicillium expansum and so on, and has wide bacteriostasis spectrum.

The invention discloses a method for improving the salt resistance of turf by adopting a reinforced garbage compost microbial agent. The reinforced salt resistant microbial agent comprises the following components: reinforced bacillus subtilis, reinforced lysine bacillus and reinforced penicillium chrysogenum, with the weight ratio of 1: 1: 1. The method disclosed by the invention adopts the following steps: gradually increasing the concentration of NaCl to reinforce microorganisms in urban garbage compost, and finally performing the screening so as to obtain the prepared reinforced salt resistant microbial agent. The obtained reinforced salt resistant microorganisms of the reinforced bacillus subtilis, the reinforced lysine bacillus and the reinforced penicillium chrysogenum are compounded into composite microbial agents in different concentrations, the composite microbial agents are inoculated to a planting system of the turf, so that the optimal concentration of the composite reinforced salt resistant microbial agent is screened out, the salt resistance of the turf is improved, and the technical support is provided for planting the carpet turf on the substrate of saline-alkali soil.

The invention belongs to the microorganism field. A solid culture medium taking Ca3(PO4)2 as a unique phosphorus source is utilized for screening a strain 14 with a phosphorus-dissolving effect, and the phosphorus-dissolving capacity of a to-be-tested phosphorus-dissolving strain is determined by virtue of an inorganic phosphorus liquid culture medium shake flask experiment. The inorganic phosphorus liquid culture medium shake flask experiment determines that the phosphorus-dissolving capacities of penicillium strains QM-6 and QM-10 are relatively high, and the maximum soluble phosphorus contents of the two corresponding strains are respectively 1239.21mg/L and 1386.93mg/L. The strains QM-6 and QM-10 are preliminarily determined as Penicillium oxalicum by virtue of an ITS sequence determination method. The determination of a greenhouse pot culture phosphorus-dissolving experiment and the analysis of a growth-promoting effect find that the phosphorus-dissolving strains QM-6 and QM-10 are capable of converting unavailable phosphorus which is difficult to be absorbed and utilized by plants in soil into soluble available phosphorus which can be directly absorbed and utilized and well promoting the growth of the plants and have important theoretical and practical significances to reduction of the application amount of phosphorus fertilizers.

The invention relates to a biochemical preparation for treating glutamic acid fermentation sewage. The biochemical preparation contains a physical adsorbent and a composite microbial preparation, wherein the composite microbial preparation is prepared from the following raw materials in parts by weight: 6-8 parts of azotobacter chroococcum, 4-6 parts of Rhodococcus sp., 4-6 parts of bacillus amyloliquefaciens, 3-5 parts of clostridium papyrosolvens, 3-5 parts of penicillium ochrochlorron, 3-4 parts of Pseudomonas stutzeri and 2-3 parts of bacillus cereus. The biochemical preparation contains a variety of microbes which have excellent degradation capability to refractory contaminants, and all strains are reasonably combined, so that the biochemical preparation has a good degradation effect and has a broad application prospect.

Methods for an improved culturing of the fungus Penicillium chrysogenum, in particular strain KIP 3201, for an optimised production of the anti-tumour natural compound sorbicillactone A (2), and derivatives thereof in large amounts

as well as optimised methods for the recovery and purification thereof from the fungal biomass and from the culture medium are described. Furthermore, the biological activity of sorbicillactone A (2), and studies regarding the genotoxicity of the compound are described.

The invention provides a penicillium strain and use thereof. The eucalyptus endophyte strain is Penicilliumsp. strain 2 of which the collection number is CGMCC No.4278. When inoculated onto the eucalyptus, the eucalyptus endophytes can relieve the aluminum toxicity in the eucalyptus. In the invention, an eucalyptus endophyte strain is screened out by separation, the relieving effect and influences of the strain on aluminum toxicity are proved, and as the aluminum toxicity becomes more and more serious with a large area of soil in China suffering from damage of acid rain, the improvement on the aluminum toxicity resistance in plants by inoculating endophytic fungi onto the plants represents a new method, and thus, a new way for lowering the aluminum toxicity in the eucalyptus is found.

The invention discloses bifidobacterium adolescentis capable of inhibiting filamentous fungi and an application of the bifidobacterium adolescentis, and belongs to the technical field of biology. Theinvention provides a bifidobacterium adolescentis CCFM1108 strain, and the strain can inhibit filamentous fungi. Inhibition of filamentous fungi is specifically reflected in that after the strain is co-cultured with penicillium expansum spores, aspergillus niger spores, penicillium roqueforti spores or penicillium digitatum spores, the germination rate of the penicillium expansum spores, aspergillus niger spores, penicillium roqueforti spores or penicillium digitatum spores can be remarkably inhibited within 7 days; and after the fermentation supernatant of the strain is separately co-culturedwith penicillium expansum spores, aspergillus niger spores, penicillium roqueforti spores or penicillium digitatum spores, germination of the penicillium expansum spores, aspergillus niger spores, penicillium roqueforti spores or penicillium digitatum spores can be remarkably inhibited, and the spore germination inhibition rates can reach 99.88+/-0.66%, 92.23+/-0.53%, 88.83+/-0.82% and 99.25+/-0.62% respectively.

The invention discloses a recombinant penicillium chrysogenum gene engineering strain and a construction method and an application thereof. The sequence of the Pc-somA gene in the penicillium chrysogenump gene engineering strain is replaced by the Bleo resistance gene. The penicillium chrysogenum is ATCC48271; the construction method is as follows: (1) extracting genomic DNA of penicillium chrysogenum; (2) constructing a gene knockout fragment; (3) preparing protoplasts of penicillium chrysogenum; and(4) introducing the gene knockout fragment into the prepared protoplast for homologous recombination to obtain recombinant penicillium chrysogenum gene engineering strain. Beneficial effects are as follows: the gene engineering strain provided by the invention can increase the biofilm amount OD570 of penicillium chrysogenum by nearly 2 times in the process of immobilized fermentation for penicillin production, the hypha can be better adsorbed and combined with a plastic medium, the titer of penicillin is increased to 1963.25 U/mL, the whole fermentation period is shortened by 24 hours, and the production cost is reduced.