Eucalyptus endophyte and application thereof

An endophyte, eucalyptus technology, applied in the application, biocide, microorganism and other directions, to achieve the effect of improving the utilization rate, reducing the utilization rate and promoting normal growth

Inactive Publication Date: 2011-02-16
FUJIAN AGRI & FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Endophytic bacteria that can promote phosphorus uptake have not been reported yet

Method used

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  • Eucalyptus endophyte and application thereof
  • Eucalyptus endophyte and application thereof
  • Eucalyptus endophyte and application thereof

Examples

Experimental program
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Embodiment 1

[0033] 1 Bacteria solution preparation

[0034] The separation and purification of bacterial strain 1 of the present invention include:

[0035] A, material: collect the roots of the wild Eucalyptus dunnen and Eucalyptus eucalyptus with a diameter of less than 0.5cm, stem segments and mature leaves with a diameter of less than 0.5cm;

[0036] B. Disinfection: leaves are sterilized by soaking in 70% alcohol for 40s and 0.1% mercury liter for 90s; stems are disinfected by soaking in 70% alcohol for 30s and 0.1% mercury liter for 60s; roots are disinfected by soaking in 70% alcohol for 50s and 0.1% mercury liter Soak for 120s;

[0037] C, separation: the roots, stem segments and mature leaves of the surface-sterilized wild Eucalyptus dunnensis and Eucalyptus macrophylla plants are inserted into the fungal solid medium to propagate the endophytic fungi;

[0038] Fungal solid medium: use improved Martin solid medium, the formula is peptone 5.0g / L, yeast extract powder 2.0g / L, glu...

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Abstract

The invention provides a penicilliums p. strain and application thereof. Eucalyptus endophyte is a penicilliums p. strain 1, and the collection number is CGMCC No.4297. The eucalyptus endophyte inoculated to eucalyptuses can improve the low phosphorus stress resistance of the eucalyptuses and promote phosphorus absorption of the eucalyptuses. Because the eucalyptus endophyte is used for promoting the phosphorus absorption of the eucalyptuses, and when the strains are inoculated to the eucalyptus plants, the activities of the plant phosphatase and the soil phosphatase can be improved, the pH value of the soil rhizosphere can be reduced, and the plant P utilization rate is greatly improved; therefore, the purpose of promoting normal growth of the plants in the low phosphorus soil is fulfilled, and the utilization rate of the soil phosphate fertilizer is greatly reduced.

Description

technical field [0001] The invention relates to a eucalyptus endophyte and its application. Background technique [0002] Soil phosphorus deficiency has become the primary factor restricting the development of agriculture in the world, with 1.319 billion hm 2 Phosphorus deficiency accounts for 43% of cultivated land. Two-thirds of the soil in my country's farmland is severely deficient in phosphorus, and the soil in the south is one of the soils with the lowest phosphorus content. If relying solely on phosphorus in the soil, it can only meet 1% to 0.5% of the needs of a season of crops. In most soils, the diffusion ability of phosphorus in the soil is poor, generally concentrated in the soil surface, and decreases with the increase of depth. Studies by Yan Xiaolong and others have shown that the current utilization rate of phosphorus fertilizers is only 0% to 25%, which means that 75% to 90% of phosphorus is converted into difficult-to-absorb phosphate, and the soil has f...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/04A01P21/00C12N1/14C12R1/80
Inventor 洪伟吴承祯谢安强
Owner FUJIAN AGRI & FORESTRY UNIV
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