33 results about "Goniothalamus" patented technology

The invention provides a bushy knotweed leaf fingerprint HPLC method. The method comprises preparation of a sample to be detected and HPLC detection of the sample. The method can simultaneously detect 12 index components such as gallic acid, chlorogenic acid, ferulic acid, polydatin, rutin, isoquercitrin, quercitrin, resveratrol, quercetin, rheum emodin, chrysophanol and physcion in bushy knotweed leaf raw drugs and their products. The bushy knotweed leaf fingerprint HPLC method has a chromatographic peak resolution, realizes retention time stabilization of different samples and solves the bushy knotweed leaf capsule quality control problems.

The invention discloses a beta-glucosidase high-yielding strain. The beta-glucosidase high-yielding strain is screened from the nature, with fructus gardenia as a carbon source, and has a preservation number of CCTCC No.M2013634 and is classified and named as penicillium rolfsii HXL; the strain is able to efficiently convert geniposide and polydatin into genipin and resveratrol; on that basis, a method for converting and preparing genipin and resveratrol by using the beta-glucosidase high-yielding strain above is created. The method has the advantages that geniposide and polydatin are respectively hydrolyzed under a mild condition, so as to avoid the shortage of the loss of active ingredients of the traditional method, and thus the yield is effectively increased; in addition, the active ingredients, namely, geniposide or polydatin, of fructus gardenia or polygonum cuspidatum are converted by mass under an in-vitro controllable condition by the modern microbial fermentation engineering technology and enzyme engineering technology, and thus the production cost is decreased, and the production efficiency is improved. With the adoption of the beta-glucosidase high-yielding strain, a feasible manner is provided for industrially producing beta-glucosidase, genipin and resveratrol as mass, large economic benefit and social benefit are brought, and the market development prospect is wide.

The present invention relates to a composition for preventing or treating respiratory diseases, and more particularly, to a pharmaceutical composition, and to a health food, comprising extracts of herbal mixtures or fermented products thereof having lactic acid bacteria as effective ingredients for preventing or treating respiratory diseases, wherein the herbal mixtures comprise: Sophora flavascens, Radix glycyrrhizae, Flos Lonicerae, Angelicae Gigantis radix, Aralia continentalis, Inula helenium, Saposhnikoviae radix, Zizyphus spinosa, Houttuynia cordata, Forsythiae fructus, Arctium lappa, Herba epimedii, ginseng, Lithospermi radix, Sanguisorbae radix, Cnidii rhizoma, Scrophulariae radix, and Polygoni cuspidati radix. The herbal extracts or fermented products thereof having lactic acid bacteria exhibit the effects of significantly lowering an airway resistance value; reducing the numbers of leukocytes, neutrophilic leukocytes, lymphocytes, eosinocytes, and basophil leukocytes to levels similar to that of a normal group; and reducing the infiltration of inflammatory cells and eosinocyte cells near AHR on lung tissues. Therefore, the herbal extracts or fermented products thereof having lactic acid bacteria may be valuably used for the prevention and / or treatment of respiratory diseases such as asthma.

The invention discloses a gingko-polygonum cuspidatum juice drink and a preparation method thereof. In the preparation method, a gingko extract and a polygonum cuspidatum extract resveratrol extracted from natural plant gingko and polygonum cuspidatum, and a fruit juice obtained by squeezing natural fruits are adopted as main materials to prepare the gingko-polygonum cuspidatum juice drink. The method comprises the following steps of: taking water as a base material, and mixing with the gingko extract, the polygonum cuspidatum extract resveratrol, honey, rock candy, the juice and an emulsion stabilizer. The juice drink is prepared by the extracts and squeezing substances of green natural products; the juice drink not only is fresh in taste and fruity, but also has special effects on preventing cardiovascular and cerebrovascular diseases, protecting the liver, beautifying, preventing tumors, and building bodies; and the juice drink has good social benefits.

The invention relates to a polygonum cuspidatum stem endophytic fungus of which the category name is penicillium oxalicum, wherein the endophytic fungus is conserved in the China General Microbiological Culture Collection Center on January 17, 2011 and the CGMCC NO is 4559. The endophytic fungus is inoculated in the culture medium of polygonum cuspidatum extract so that polydatin in polygonum cuspidatum can be converted to resveratrol. Therefore, polydatin can be directly converted to resveratrol through microbial fermentation, the extraction of polydatin is not required, the cost can be saved and environmental pollution can be reduced.

The invention discloses a Polygonum cuspidatum root endophytic strain. The strain is named Penicillium oxalicum G3 in a classified way, the strain was collected in China General Microbiological Culture Collection Center on January 17, 2011, and the collection number is CGMCC NO.4558. Polydatin in Polygonum cuspidatum can be transformed into resveratrol by inoculating the strain into a culture medium containing Polygonum cuspidatum boiling extracting solution, so that the polydatin can be directly transformed into the resveratrol through microbial fermentation, the extraction of the polydatin is not required, the cost can be saved, and the pollution to the environment can be reduced.

The invention belongs to the field of biotechnology, relates to a method for induction of polygonum cuspidatum trichoid root and enlarged cultivation by use of a biological reactor, and in particular relates to a method for production of resveratrol from the polygonum cuspidatum trichoid root and enlarged cultivation. According to the method, polygonum cuspidatum tissue culture seedlings are induced successfully from polygonum cuspidatum plant wild stem sections with buds as explants, a polygonum cuspidatum tissue culture system is established, a polygonum cuspidatum trichoid root system is successfully induced, a polygonum cuspidatum trichoid root resveratrol production system is established, the resveratrol is successfully produced from the polygonum cuspidatum trichoid root by an airlift bioreactor, the resveratrol content is determined, and the resveratrol extracted and separated. Through the production of the resveratrol from the polygonum cuspidatum trichoid root, compared with the current mode of production, the method for the production of the resveratrol from the polygonum cuspidatum trichoid root has the rapid growth characteristic on a hormone free cultivation medium, so that the method has the advantages of simple operation, low cost, no limitation of climate, lands and other natural conditions, and the like, and a reliable source and material basis for the future industrial production is provided.

The invention provides a bacterial strain capable of producing glycosidase, namely Aspergillus niger XW-2, and application thereof in improving resveratrol content in Japanese knotweed in the biotransformation method. The bacterial strain is preserved in the Chinese typical culture preservation center, the preservation number is CCTCC No: M2011358, and the preservation date is 2011 / 10 / 19. The Aspergillus niger capable of producing glycosidase and the application thereof in improving resveratrol content in Japanese knotweed have the advantages that: (1) Aspergillus niger is normally-used strains in the fermentation industry and is non-toxic, harmless and safe to use; (2) Aspergillus niger XW-2 is simple in nutrition requirements, fast in growth and easy to culture; (3) glycosidase producedby Aspergillus niger XW-2 is extracellular enzyme, after thalli are removed through separation, coarse enzyme liquid directly serves as a biotransformation system, and after Japanese knotweed is grinded and added into the coarse enzyme liquid for transformation, content of resveratrol in Japanese knotweed can be improved by at most four times; and (4) Japanese knotweed after biotransformation treatment contains no thalli or metabolite residues of thalli, so that Japanese knotweed can be used as Chinese herbal medicines and can be used for extraction and preparation of resveratrol.

The invention relates to olygonum cuspidatum endogenous bacterial strain for converting polydatin. The olygonum cuspidatum endogenous bacterial strain is classified and named as aspergillus fumigates J3 (Aspergillus fumigates ) bacterial strain. The olygonum cuspidatum endogenous bacterial strain had been preserved in China Center for Type Culture Collection in March 25, 2015, and the collecting umber is CCTCC M2015161. Biotransformation fermental cultivation is conducted on the bacterial strain in a PDB culture medium with 10 mg substrate polydatin. A biotransformation product component B possesses ability of eliminating DPPH. free radical and OH free radical in vitro after being separated and purified, and the capability to elimite OH free radical of the biotransformation product component B is greater than that of substrate polydatin. In this way, the bacterial strain J3 can conduct biotransformation on polydatin, ramification with higher antioxidant activity is formed, and more lead compound is provided for screening of natural antioxidant activity drug.

The invention discloses polygonum cuspidatum flavored fermented bean curd. Polygonum cuspidatum with a health-care effect serves as a raw material, and the polygonum cuspidatum flavored fermented bean curd is prepared by the following processes of pulping, mixing the pulp, forming a blank, inoculating, salting, canning, checking and the like. Health-care ingredients of the polygonum cuspidatum are mixed into the fermented bean curd, the finished polygonum cuspidatum flavored fermented bean curd has the effects of clearing away heat and removing toxic materials, dispelling the wind and promoting diuresis, relieving cough and reducing sputum, strengthening Yang and replenishing vital essence and is enriched in various amino acids, unsaturated fatty acids, vitamins and mineral substances necessary for the human body. The finished product is rich in nutrition, fine and smooth in texture, delicate in taste, and when the fermented bean curd is eaten together with rice or bread, the appetite can be improved, and digestion is promoted. When the polygonum cuspidatum is comprehensively developed, a wide market space is provided for medicinal herb growers who plant the polygonum cuspidatum.

The invention relates to a plant growth regulator extracted from a Polygonaceae plant giant knotweed and application of the plant growth regulator, and belongs to the technical field of agro-ecology. The giant knotweed multifunctional plant growth regulator is prepared by the steps of: extracting from the giant knotweed, concentrating, drying and adding a solvent. Through related experiments, thegiant knotweed used as the plant growth regulator has remarkable effects, namely a giant knotweed extract at lower concentration has an effect of promoting plant growth and a giant knotweed extract at higher concentration has a certain effect of inhibiting plant growth.

The invention provides a method for cultivating polygonum cuspidatum seedlings, which comprises the steps of cultivating aseptic seedlings, cultivating multiple shoots, cultivating strong seedlings, rooting, acclimatization and transplant. During the step of cultivating aseptic seedlings, a polygonum cuspidatum tender stem / shoot is adopted as an explant. The polygonum cuspidatum tender stem / shoot is sterilized by alcohol, disinfected by 0.05-0.15% of HgCl2, rinsed by sterile water, inoculated in a 1 / 2 MS or an MS with a moderate amount of plant growth hormone, and cultivated for 12-18 days to obtain aseptic seedlings. During the step process of cultivating multiple shoots, an already grown aseptic seedling is cut off, inoculated in a multiple shoot culture medium prepared through adding a moderate amount of plant growth hormone in a 1 / 2 MS, and cultivated. During the step of strong seedling cultivating and rooting, when multiple shoots are grown to about 1 cm in size, the multiple shoots are inoculated into strong seedling culture media and rooting culture media, grouped in bottles and cultivated, where in the culture media are pared by 1 / 2 MS and / or MS with a moderate amount of plant growth hormone. The above steps are conducted at the cultivating temperature of 15-30 DEG C and under the illumination intensity of 2000-3000 LUX, wherein the illumination time is 6-12 hours / day. During the step of acclimatization and transplant, regenerated plants from root cultures are transplanted in a matrix composed of garden soil and peat in the ratio of 2:1, shaded by a shading net, and grow out of the matrix after being cultivated at the humidity of 60-70% for 15-30 days. According to the invention, the growth speed and the plantation benifit of polygonum cuspidatum seedlings are greatly improved. meanwhile, the method is low in cost, small in pollution, simple in process, applicable in all seasons and suitable for large-scale culture of seedlings.

The invention relates to a method for preparing root systems containing resveratrol with culturing of polygonum cuspidatum leaves. Adventitious roots and hairy roots are directly obtained through the polygonum cuspidatum leaves and callus tissues thereof, then the adventitious roots and the hairy roots are used as raw materials, and the resveratrol is extracted from the raw materials. The method comprises the following steps: (1) preparing bacteria-free polygonum cuspidatum seedlings; (2) preparing different explant callus tissues of polygonum cuspidatum; (3) performing direct differentiation and rooting on the callus tissues of the polygonum cuspidatum so as to obtain polygonum cuspidatum root systems. Therefore, a new way for replacing wild polygonum cuspidatum resources is provided.

The invention discloses a mountain chicken feed containing giant knotweed rhizome residue, the mountain chicken feed is prepared by adding the giant knotweed rhizome residue into a basic ration for mountain chickens, wherein the adding amount of the giant knotweed rhizome residue is 5wt%-15wt% of the basic ration for the mountain chickens. The invention further discloses a preparation method of the mountain chicken feed. The mountain chicken feed provided by the invention is prepared through the following steps: performing drying and crushing processing treatment on waste residue obtained by extracting juice from giant knotweed rhizome; then preparing giant knotweed rhizome residue powder; and finally adding the giant knotweed rhizome residue powder into the basic ration for the mountain chickens. The mountain chicken feed can be used for improving the health condition of the mountain chickens, improving the chicken quality and flavor of the mountain chickens, reducing the feeding cost of the mountain chickens and upgradng the economic benefits of the mountain chickens.

The invention relates to a method for preparing root systems containing resveratrol with culturing of polygonum cuspidatum leaves. Adventitious roots and hairy roots are directly obtained through the polygonum cuspidatum leaves and callus tissues thereof, then the adventitious roots and the hairy roots are used as raw materials, and the resveratrol is extracted from the raw materials. The method comprises the following steps: (1) preparing bacteria-free polygonum cuspidatum seedlings; (2) preparing different explant callus tissues of polygonum cuspidatum; (3) performing direct differentiation and rooting on the callus tissues of the polygonum cuspidatum so as to obtain polygonum cuspidatum root systems. Therefore, a new way for replacing wild polygonum cuspidatum resources is provided.

The invention discloses a method for increasing the yield of resveratrol by fermenting Aspergillus niger. The Aspergillus niger species is cultured in the culture medium of the Erlenmeyer flask for 48 hours, and the mycelium is evenly added to the moistening medium according to the mass percentage of 2%. : 1 in the Rhizome of Polygonum cuspidatum, stacked and fermented for 30h at a constant temperature of 40°C. The invention adopts a solid accumulation fermentation method to convert the polydatin in Polygonum cuspidatum into resveratrol, and optimizes the solid fermentation conditions. This method does not have a strong acid-base reaction, does not need crude glycoside extraction, and does not need to add expensive equipment, so it is easy to popularize. The resveratrol extraction yield of the present invention is increased to 1.197%, which is 30% higher than that of the existing method, and a very significant technical effect has been achieved.

The invention belongs to the field of traditional Chinese medicine health-caring, and particularly relates to polygonum cuspidatum instant tea and a preparation method thereof. The polygonum cuspidatum instant tea is prepared from the following raw materials in parts by weight: 2-10 parts of a polygonum cuspidatum extract, and 20-40 parts of a black tea extract. The preparation method of the polygonum cuspidatum instant tea comprises the following steps: respectively filtering the the polygonum cuspidatum extract and the black tea extract by using ultra-filtration membranes of which the molecular weight is 5000-7000; concentrating the filtrates by carrying out reverse osmosis until the solid contents are 40-50%; uniformly mixing the two concentrated components; and freeze-drying the mixture. The polygonum cuspidatum instant tea has improved taste. Being tasted, the polygonum cuspidatum instant tea is free of bitter and astringent taste, as well as free of traditional Chinese medicine smell. Thus, the polygonum cuspidatum instant tea is suitable for long-term drinking. Moreover, the black tea is prepared by carrying out fermentation with tannase, so that the bitter and astringent taste of the black tea is thereby reduced. The tea beverage prepared by brewing the polygonum cuspidatum instant tea is clear and free of precipitation; thus, the polygonum cuspidatum instant tea is apt to be drunk every day.

The invention relates to the technical field of plant polysaccharides, and discloses a water-soluble polygoni cuspidate rhizoma et radix polysaccharide extract and a preparation method thereof. A water-soluble polygoni cuspidate rhizoma et radix polysaccharide has good anti-inflammation, analgesic and immunity regulation effects. The water-soluble polygoni cuspidate rhizoma et radix polysaccharide extract is obtained by the preparation method comprising the following steps: 1, degreasing; 2, water extraction; 3, deproteinization; 4, ethanol precipitation; 5, drying. A method for extracting the water-soluble polygoni cuspidate rhizoma et radix polysaccharide is provided, the shortcoming of gaps about the water-soluble polygoni cuspidate rhizoma et radix polysaccharide and the preparation method for the same in the field of plant polysaccharides is overcome, and a basis is provided for post development and utilization of the water-soluble polygoni cuspidate rhizoma et radix polysaccharide.

The invention provides a strain of Inonotus obliquus QD04 and a method for transforming Polygonum cuspidatum to produce resveratrol, triterpene saponins and polysaccharides. The Inonotus obliquus QD04 is preserved in the China Type Culture Collection Center, numbered CCTCC M 2015036 , the present invention uses the medicinal fungus QD04 to convert resveratrol glycosides in plants into resveratrol, which greatly improves the yield of resveratrol, with simple process and good repeatability; the bacteria can use Polygonum cuspidatum to synthesize its own The active substances are triterpene saponins and polysaccharides; the fermentation product Inonotus obliquus mycelium is rich in essential amino acids and has high nutritional value; resveratrol, triterpene saponins, polysaccharides and obliquus obliquus can be obtained at the same time in one fermentation process Four kinds of mycelia products have high added value in the production process; the Inonotus obliquus can more thoroughly hydrolyze the cellulose of Polygonum cuspidatum, fully utilize the biomass resources of Polygonum cuspidatum, and reduce waste of resources and environmental pollution.

The invention relates to a medium composition for cultivating pleurotus cornucopia by Japanese ampelopsis roots. The medium composition comprises, by weight, 40-80 parts of Japanese ampelopsis rhizomes, 20-40 parts of Japanese fleecceflower rhizome waste, 15-35 parts of Thomson kudzuvine root filter residues, 0.3-0.6 part of cane sugar, 0.5-1.5 parts of urea and 0.5-1.5 parts of gypsum. The medium composition further provides a method for cultivating the pleurotus cornucopia. The method includes the steps: traditional Chinese medicinal material pretreatment; medium sterilization; mushroom inoculation; cultivation; alcohol extract treatment; cultivation; pleurotus cornucopia harvesting. According to the medium composition, functional components of the Japanese ampelopsis roots are sufficiently utilized, the functions of activating blood and protecting health of the pleurotus cornucopia are enhanced, cellulose in existing Japanese ampelopsis root and Thomson kudzuvine root waste is sufficiently utilized, and the utilization rate of traditional Chinese medicinal material waste is increased.

The present invention describes extracts of Polygonum cuspidatum and related compositions that are capable of photodynamic inactivation (PDI) of microorganisms such as bacteria, viruses, fungi, and protozoa, and capable of killing or inactivating cancer cells. The present invention further describes methods of using said Polygonum cuspidatum extracts and photodynamic inactivation (PDI) as a therapy for the treatment of microbial infection and as well as the use of Polygonum cuspidatum extract and related compositions as photodynamic therapy agents for the treatment of cancer.

The invention provides a bushy knotweed leaf fingerprint HPLC method. The method comprises preparation of a sample to be detected and HPLC detection of the sample. The method can simultaneously detect 12 index components such as gallic acid, chlorogenic acid, ferulic acid, polydatin, rutin, isoquercitrin, quercitrin, resveratrol, quercetin, rheum emodin, chrysophanol and physcion in bushy knotweed leaf raw drugs and their products. The bushy knotweed leaf fingerprint HPLC method has a chromatographic peak resolution, realizes retention time stabilization of different samples and solves the bushy knotweed leaf capsule quality control problems.

The invention discloses guava buccal tablets. The guava buccal tablets are prepared from the following raw materials in parts by weight: 2-6 parts of cochinchinese asparagus roots, 5-10 parts of wild radix puerariae, 2-5 parts of giant knotweed rhizomes, 1-3 parts of three-coloured amaranth, 5-10 parts of fiveleaf gynostemma herbs, 15-20 parts of guavas, 2-4 parts of green tea leaves, 2-3 parts of apple peels, 5-10 parts of carrots, 2-4 parts of spinach, 30-40 parts of starch and 20-30 parts of xylitol. The buccal tablets disclosed by the invention are rich in nutrition, have the efficacies of benefitting qi, invigorating the spleen, eliminating phlegm, stopping a cough, clearing heat and removing toxicity and are especially suitable for diabetics to hold in mouths.

The invention relates to the field of pharmaceutical formulations, in particular to an anti-hepatic-fibrosis traditional Chinese medicinal decoction. The anti-hepatic-fibrosis traditional Chinese medicinal decoction is characterized by comprising, by weight, 17-22 parts of silybum marianum, 13-17 parts of prince's-feather fruits, 17-22 parts of desmodium, 3-7 parts of abrus herbs, 13-17 parts of urena procumbens, 17-22 parts of the root of tubeflower duchmans pipe, 17-22 parts of polygonum polystachyum, 13-17 parts of polygonum capitatum, 17-22 parts of Chinese starjasmine stems, 22-28 parts of ground beetles, 13-17 parts of lomatogonium rotatum, 3-7 parts of leeches, 13-17 parts of giant knot weeds, 8-12 parts of the root of Chinese thorowax, 8-12 parts of peach kernels, 5-10 parts of Chinese honeylocust spines, 13-17 parts of roasted turtle shells, 8-12 parts of chicken's gizzard-membranes, 8-12 parts of astragalus membranaceus and 17-22 parts of madder. The raw materials are soaked in water, and dregs are removed after decoction to obtain a filtrate. The anti-hepatic-fibrosis traditional Chinese medicinal decoction can eliminate inflammatory cells and inflammatory media of hepatic inflammations and has remarkable curative effect on hepatic fibrosis caused by hepatitis virus infection.

The invention discloses a processing method of polygonum cuspidatum-bamboo shoot having hot pepper oil. The processing method comprises: S1) raw material treatment: washing fresh bamboo shoots by using clear water, and cutting the washed bamboo shoots into thin slices by using a cutter; S2) cooking; S3) material preparation comprising the preparation of a hot pepper oil material and a sousing liquid containing a traditional Chinese medicine composition, wherein the traditional Chinese medicine composition comprises, by weight, 30-50 parts of polygonum cuspidatum, 20-40 parts of dodder, 20-35 parts of dendrobium, 10-20 parts of panax notoginseng, and 10-30 parts of wild chrysanthemum; and S4) sousing: placing the bamboo shoots into a jar, sprinkling a layer of zanthoxylum bungeanum maxim and monosodium glutamate on the surface of the bamboo shoot, sealing the jar opening by using a plastic bag, taking out after 30-50 days, pouring the obtained bamboo shoot into a basin, adding the materials, and uniformly stirring. The polygonum cuspidatum-bamboo shoot having hot pepper oil of the present invention has characteristics of excellent taste and high nutrition value.