Bushy knotweed leaf fingerprint HPLC method and its use in bushy knotweed leaf capsule quality control

A technology of fingerprint spectrum and Polygonum cuspidatum, which is applied to the HPLC method of Polygonum cuspidatum fingerprint and its application in the quality control of Polygonum cuspidatum capsules, can solve problems such as lack of scientific basis, achieve high separation of chromatographic peaks, and solve the problem of quality control. , the effect of stable retention time

Active Publication Date: 2016-10-12
云南海沣药业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current summary of the indications and efficacy of Polygonum cuspidatum leaves is still the experience accumulated in practice. It is in the stage of empirical medicine, and there is still a lack of corresponding scientific basis for the safety, effectiveness, dosage, and usage of Polygonum cuspidatum leaves.

Method used

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  • Bushy knotweed leaf fingerprint HPLC method and its use in bushy knotweed leaf capsule quality control
  • Bushy knotweed leaf fingerprint HPLC method and its use in bushy knotweed leaf capsule quality control
  • Bushy knotweed leaf fingerprint HPLC method and its use in bushy knotweed leaf capsule quality control

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Embodiment 1 Establishment of Polygonum cuspidatum Leaf Fingerprint HPLC Method

[0022] 1. Sample

[0023] Table 1 List of sample information

[0024]

[0025] 2. Reference substance

[0026] Table 2 List of reference substance information

[0027]

[0028] 2 sample preparation

[0029] 2.1 The medicinal material Polygonum cuspidatum No. 1-3, weigh 0.2500g to 100mL in a ground-mouth Erlenmeyer flask, add 20mL of 80% methanol, weigh, ultrasonicate (power 200W, frequency 40kHz) for 30min, make up the weight, and centrifuge for 10min. spare.

[0030] 2.2 Reference substance Take quercitrin reference substance, accurately weigh 1.73mg into a 10mL brown volumetric flask, add methanol to make a solution containing 0.173mg per mL, and obtain it. Take polydatin, resveratrol, quercetin and emodin reference substances, accurately weigh 5.70, 5.36, 5.39, and 5.61mg, put them into 25mL brown volumetric flasks, add methanol to make each mL contain 0.2280, 0.2144, 0.2156 ...

Embodiment 2

[0043] The UPLC-MS research of embodiment 2 Polygonum cuspidatum leaf medical material

[0044] 1. Sample

[0045] Polygonum cuspidatum No. 3 medicinal material sample.

[0046] 2. UPLC-MS conditions

[0047] 2.1 UPLC gradient conditions

[0048] Utilize Shimadzu Method Tranfer software to convert (column temperature: 20 ℃) ​​into UPLC condition (column temperature: 20 ℃) ​​in the HPLC method among the embodiment 1,

[0049] 2.2 Mass Spectrometry Conditions

[0050]

[0051]

[0052] 3. Results and Analysis

[0053] Through the UPLC-MS detection of Polygonum cuspidatum No. 3 sample, 20 components were preliminarily identified after analysis, as shown in Table 4.

[0054] Table 4 Retention time of main components, MS data and identification of compounds

[0055]

Embodiment 3

[0056] Example 3 Fingerprint Spectrum Research of Polygonum cuspidatum leaves and capsules

[0057] 1. Sample name

[0058] Fingerprint analysis samples include 6 batches of Polygonum cuspidatum leaves, 2 batches of Polygonum cuspidatum leaves and 3 batches of Polygonum cuspidatum capsules, see Table 1 in "Samples and Reference Substances in Example 1".

[0059] 2. Sample preparation

[0060] 2.1 The medicinal material is the medicinal material solution prepared in the optimization of HPLC conditions and the content determination in the content determination in Example 1.

[0061] 2.2 The finished product adopts the finished product solution prepared in “Optimization of HPLC Conditions in Example 1 and Content Determination in Content Determination”.

[0062] 3.3 Reference substance polydatin, quercetin, resveratrol, quercetin and emodin mixed reference solution, the concentrations are 0.0570, 0.0346, 0.0536, 0.0539, 0.0561mg / mL; the rest of the reference substances are diss...

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Abstract

The invention provides a bushy knotweed leaf fingerprint HPLC method. The method comprises preparation of a sample to be detected and HPLC detection of the sample. The method can simultaneously detect 12 index components such as gallic acid, chlorogenic acid, ferulic acid, polydatin, rutin, isoquercitrin, quercitrin, resveratrol, quercetin, rheum emodin, chrysophanol and physcion in bushy knotweed leaf raw drugs and their products. The bushy knotweed leaf fingerprint HPLC method has a chromatographic peak resolution, realizes retention time stabilization of different samples and solves the bushy knotweed leaf capsule quality control problems.

Description

technical field [0001] The invention relates to a method for quality control of traditional Chinese medicines, in particular to an HPLC method for fingerprints of polygonum cuspidatum leaves and its application in quality control of polygonum cuspidatum capsules. technical background [0002] Polygonum cuspidatum Sieb.et Zucc., a Polygonum cuspidatum Sieb.et Zucc., is mainly distributed in temperate Asia. In China, it is widely distributed in the Qinling Mountains, south of the Yangtze River, reaching the Nanling Mountains and southwest, and northeast reaching North Korea, Korea, and Japan. It is mostly evergreen below 2000m. Forest or mesophytic mixed forest next to the stream, with strong environmental adaptability. The traditional Chinese medicine Polygonum cuspidatum is the dry root and rhizome of P.cuspidatum. The effect. In "Rescue Famine Materia Medica", it is recorded that the sour barrel bamboo shoots (polygonum cuspidatum leaves) are "on the side of the Shaohua M...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
CPCG01N30/02G01N2030/027
Inventor 陈艳明罗维早王欣覃瑶
Owner 云南海沣药业有限公司
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