The invention belongs to the technical field of preparation of
protein peptides, and particularly relates to a
ginkgo peptide with anti-inflammatory and anti-oxidation effects and a preparation method of the
ginkgo peptide. According to the present invention, with the process of
sodium carbonate-
citric acid-hot water three-stage pretreatment, composite
fermentation and double enzymolysis, the toxic components in the
ginkgo can be efficiently removed, the complex structure of the ginkgo can be disintegrated, the macromolecular
protein can be efficiently converted into the
small molecule peptide, and the rich specific
active components of the ginkgo can be retained; therefore, in the ginkgo peptide provided by the invention, the residual amount of
ginkgolic acid is less than or equal to 10mg / kg, the residual amount of
cyanide is less than or equal to 0.5 mg / kg, and the ginkgo peptide meets the
food safety standard; meanwhile, the
protein content in the ginkgo peptide is larger than or equal to 70%, the average molecular weight is smaller than or equal to 1000 Da, the
scavenging rate of the ginkgo peptide to
DPPH free radicals is larger than or equal to 85%, and the inhibition rates of the ginkgo peptide to
inflammatory factors TNF-alpha and IL-6 are larger than or equal to 30%, that is, the ginkgo peptide has the advantages of being high in toxic component residue removal rate, high in
protein content, reasonable in molecular
weight distribution, sufficient in anti-inflammatory and anti-oxidation
biological activity and the like; and a brand-new and efficient way is provided for high-value utilization of ginkgo resources.