Oil-control skin-astringing composition as well as preparation method and application thereof

Abstract

The invention discloses an oil-controlling and skin-astringing composition as well as a preparation method and application thereof, and particularly belongs to the field of preparations for dressing and makeup. The composition for controlling oil and astringing skin is prepared from the following components in parts by weight: 1.5 to 2.1 parts of tea leaf extract, 7.1 to 8.6 parts of hamamelis virginiana water, 0.003 to 0.01 part of moso bamboo leaf extract, 0.036 to 0.051 part of lotus corniculatus seed extract and 0.6 to 1.2 parts of alkanet extract. The oil-controlling and skin-astringing composition provided by the invention has excellent performance of inhibiting grease secretion of human sebaceous gland cells, and has an excellent deep oil-controlling effect on skin; the resident skin external preparation provided by the invention has excellent effects of short-time oil control, lasting oil control and skin astringing.

Description

Technical Field

[0001] The invention belongs to the field of cosmetic preparations, and in particular relates to an oil-controlling and skin-smoothing composition and a preparation method and application thereof. Background Art

[0002] In the field of modern skin care, oil control and pore tightening are the focus of many consumers. Excessive oil secretion and enlarged pores not only affect the appearance of the skin, but also easily cause skin problems such as acne and pimples. Therefore, it is of great significance to develop a skin care composition that can effectively control oil and tighten pores.

[0003] Witch hazel water contains vitamins, tannins, flavonoids and other beneficial ingredients, which have anti-inflammatory and anti-oxidation effects, regulate oil secretion, shrink pores, and even skin tone. Tea extract is widely used in cosmetics. Tea contains more than 1,400 substances, including tea polyphenols, purine bases, amino acids, proteins, vitamins, mineral elements and tea saponins, which have significant antioxidant, anti-inflammatory and antibacterial, pore-tightening, anti-wrinkle and anti-aging effects. Although witch hazel water and tea extract each have significant skin care effects, a single ingredient is often difficult to meet consumers' comprehensive needs for oil control and pore tightening.

[0004] Therefore, developing an oil-controlling and skin-astringing composition that organically combines multiple natural ingredients can not only give play to the advantages of each of them, but also promote the oil-controlling and skin-astringing effects of North American witch hazel water and tea extract through synergistic effects, thereby achieving more significant skin care effects. Summary of the invention

[0005] In view of the deficiencies in the prior art, the purpose of the present invention is to provide a composition with oil-controlling and skin-tightening effects, which contains multiple plant extracts including tea extract, witch hazel water, bamboo leaf extract, lotus seed extract and ginkgo root extract, as well as a preparation method and application. Through the organic combination of natural plant ingredients, a more significant oil-controlling and pore-tightening effect is achieved.

[0006] To achieve the above object, the present invention discloses the following technical solutions:

[0007] In a first aspect, the present invention provides an oil-controlling and skin-smoothing composition, which comprises the following components in parts by mass:

[0008]

[0009]

[0010] Preferably, the composition comprises the following components in parts by mass:

[0011]

[0012] Further preferably, the composition comprises the following components in parts by mass:

[0013]

[0014] More preferably, the composition comprises the following components in parts by mass:

[0015]

[0016] In a second aspect, the present invention provides the use of the aforementioned oil-controlling and skin-astringing composition in the preparation of a skin care product having oil-controlling, acne-removing, anti-oxidation, antibacterial, anti-inflammatory and / or soothing effects.

[0017] In a third aspect, the present invention provides a leave-on skin topical preparation having the effects of oil control and skin astringency, wherein the leave-on skin topical preparation comprises the aforementioned oil control and skin astringency composition;

[0018] The amount of the oil-controlling and skin-astringing composition added to the leave-on skin external preparation is 5-10 wt %.

[0019] Preferably, the leave-on skin topical preparation further comprises a thickener, a moisturizer, a preservative, a chelating agent, an emulsifier, a pH adjuster, and a solvent.

[0020] Further preferably, the thickener is selected from at least one of acrylic acid (esters) / C10-30 alkyl acrylate cross-linked polymer and carbomer;

[0021] The moisturizing agent is selected from at least one of glycerin, allantoin, 1,3-propylene glycol, butylene glycol, and 1,2-hexanediol;

[0022] The preservative is p-hydroxyacetophenone;

[0023] The chelating agent is disodium EDTA;

[0024] The emulsifier is PPG-5-cetoeth-20;

[0025] The pH regulator is arginine;

[0026] The solvent is deionized water.

[0027] In a fourth aspect, the present invention provides a method for preparing the leave-on skin topical preparation according to the third aspect, the preparation method comprising the following steps:

[0028] S1. The thickener, humectant and deionized water are stirred and mixed, heated to 80-90°C, homogenized at a speed of 1000-1500rpm for 5-10min, and kept warm for standby after homogenization to obtain component A;

[0029] S2. The preservative, chelating agent and emulsifier are stirred and mixed with deionized water, heated to 50-60°C, homogenized at a speed of 800-1000rpm for 3-5min, and kept warm for standby after the homogenization to obtain component B;

[0030] S3. Component A is cooled to 50-60°C, component B is added at a rotation speed of 500-600rpm, and the mixture is stirred and mixed for 5-10min. Then, the temperature is cooled to below 45°C, the oil-controlling and skin-smoothing composition is added at a rotation speed of 500-600rpm, and the mixture is stirred and mixed for 5-10min. Finally, a pH adjuster is added to adjust the pH to 5.5-6.5, stirring is stopped, and the material is discharged to obtain a leave-on skin topical preparation.

[0031] In the present invention:

[0032] Tea extract is rich in tea polyphenols, alkaloids, tea polysaccharides, theanine, vitamins, etc. It has antioxidant effects, can scavenge free radicals, and delay skin aging. Tea extract also has astringent properties, can shrink large pores, and reduce wrinkles. It also has antibacterial and anti-inflammatory effects. In terms of oil control, tea extract can inhibit oil secretion, reduce oiliness, and help maintain the water and oil balance of the skin.

[0033] Witch hazel water is rich in tannic acid, gallic acid, catechins, proanthocyanidins, flavonoids, choline, saponins, etc. It has the effects of tightening pores, anti-inflammatory and calming, oil control and balance, and anti-oxidation. For oily and combination skin, witch hazel water can control excess oil secretion and reduce the greasy feeling of the skin. It also has a moisturizing effect. Witch hazel water can also reduce redness and inflammation and soothe sensitive skin.

[0034] Moso bamboo leaf extract contains a variety of flavonoids, which have antioxidant, antibacterial and anti-inflammatory, blood lipid regulating and anti-cell aging effects. Moso bamboo leaf extract can help control oil, it can regulate sebum secretion and reduce oiliness of the skin.

[0035] Lotus japonicus seed extract has astringent and anti-inflammatory properties, helping to reduce oil secretion in the skin while providing soothing and moisturizing effects.

[0036] Ginkgo biloba root extract has antioxidant and anti-inflammatory effects, can reduce skin oil secretion, and has the effect of tightening pores.

[0037] The present invention has found that when tea extract, witch hazel water, bamboo leaf extract, lotus seed extract and ginkgo lithospermum root extract are used at the same time, the effects of each other can be enhanced. Specifically, tea extract and witch hazel water both have astringent and oil-control effects, and their simultaneous use can more effectively reduce sebum secretion and shrink pores; the antioxidant and anti-inflammatory properties of bamboo leaf extract and lotus seed extract can soothe the skin, reduce inflammation and oil secretion, and the like; the addition of ginkgo lithospermum root extract can further enhance the oil-control effect, and they promote each other to obtain more excellent oil-control and skin-astringent effects.

[0038] Beneficial effects of the present invention:

[0039] 1. The oil-controlling and skin-smoothing composition provided by the present invention has excellent performance in inhibiting the oil secretion of human sebaceous gland cells and has excellent deep oil-control effect on the skin;

[0040] 2. The leave-on skin topical preparation provided by the present invention has excellent short-term oil control, long-lasting oil control and skin astringent effects. Compared with the blank control group, the oil control and enlarged pores reduction effects of the test group are obvious. DETAILED DESCRIPTION

[0041] In order to make the purpose, technical scheme and advantages of the present invention more clearly understood, the present invention is further described in detail below in conjunction with specific embodiments. However, the following embodiments are only preferred embodiments of the present invention, not all. For the sake of clarity, all features of the actual embodiments are not described.

[0042] Based on the embodiments in the implementation manner, other embodiments obtained by those skilled in the art without making any creative work shall all fall within the protection scope of the present invention.

[0043] In the present invention:

[0044] Tea extract is Camellia sinensis leaf extract, INCI name / English name is Camellia sinensis LEAF EXTRACT, purchased from Yunnan Tangren Biotechnology Co., Ltd.;

[0045] The witch hazel water is witch hazel water (HAMAMELIS VIRGINIANA), INCI name / English name is HAMAMELIS VIRGINIANA (WITCH HAZEL) WATER, purchased from Ganzhou Huahan Biotechnology Co., Ltd.;

[0046] The moso bamboo leaf extract is a moso bamboo (PHYLLOSTACHYS PUBESCENS) leaf extract, INCI name / English name is PHYLLOSTACHYS PUBESCENS LEAF EXTRACT, purchased from Golden Basin Biotechnology Co., Ltd.;

[0047] Lotus corniculatus seed extract is a lotus corniculatus seed extract, INCI name / English name is LOTUS CORNICULATUS SEED EXTRACT, purchased from Shanghai Jiakai Biological Co., Ltd.

[0048] Lithospermum officinale root extract is Lithospermum officinale root extract, INCI name / English name is LITHOSPERMUM OFFICINALE ROOT EXTRACT, purchased from Mufan Biotechnology;

[0049] Other raw materials are commercially available.

[0050] Preparation of the composition:

[0051] Accurately weigh the raw materials listed in Table 1, mix and stir evenly to obtain an oil-controlling and skin-astringing composition.

[0052] Table 1 Composition raw material mass ratio

[0053]

[0054] Note: “-” in the table means no addition.

[0055] Sebum secretion inhibition test:

[0056] Test cells: Immortalized human sebaceous gland cell line SZ95 was selected. The cell line passed the cell line quality control test and the result was qualified. The generation number of this test is: P10.

[0057] Experimental consumables: fetal bovine serum, high-glucose DMEM medium, penicillin, streptomycin, phosphate buffered saline (PBS), modified Oil Red detection kit, paraformaldehyde, isopropanol, isotretinoin, dihydrotestosterone (DHT).

[0058] Complete medium: high-glucose DMEM medium supplemented with 10% fetal bovine serum, 100 U / mL penicillin, and 100 μg / mL streptomycin;

[0059] Test sample group: a complete medium containing a sample to be tested and 1 μmol / L DHT, wherein the sample to be tested is component 1-12, and the concentration of the sample to be tested in the complete medium is 5wt% and 10wt%;

[0060] Blank control group: complete medium;

[0061] Negative control group: complete medium containing 1 μmol / L DHT;

[0062] Positive control group: complete culture medium containing 1 μmol / L DHT and 5 μmol / L isotretinoin.

[0063] Test plan:

[0064] (1) Cell preparation: Well-grown human sebaceous gland cells were inoculated in complete culture medium in a 24-well plate, with 5 × 10 cells per well. 4 cells, add 500 μL PBS solution to the edge wells of the well plate, and set up a blank control group, a negative control group, a positive control group and a test sample group, with three replicate wells in each group. The 24-well plate was placed in a 37°C, 5% CO2 incubator and cultured overnight.

[0065] (2) Administration: After overnight culture, the complete medium in the 24-well plate was replaced and the administration was carried out. The blank control group was given 1 mL of complete medium, the sample group was given 1 mL of complete medium containing the corresponding concentration of the test sample and 1 μmol / L DHT, the positive control group was given 1 mL of complete medium containing 1 μmol / L DHT and 5 μmol / L isotretinoin, and the negative control group was given 1 mL of complete medium containing 1 μmol / L DHT. After administration, the 24-well plate was placed in a 37°C, 5% CO2 incubator for 48 h.

[0066] (3) Oil droplet detection: After incubation, wash with PBS, fix with 4% paraformaldehyde for 30 min, and wash with distilled water for 2 min. Wash twice and perform detection according to the instruction manual of the Oil Red Detection Kit. Use an inverted microscope and an ELISA reader to detect the oil droplets in each sample well. The data of each group are the average of three replicate wells.

[0067] Criteria:

[0068] Oil red is a fat-soluble azo dye that can specifically stain neutral lipids such as triglycerides in cells or tissues, but has a weaker staining effect on lipids such as phospholipids and steroids. When cells or tissue sections are immersed in oil red staining solution, oil red can dissolve in fat (such as lipid droplets) in cells or tissues, making them red or orange-red. The staining in tissues or cells can be observed under a microscope to compare the differences in triglyceride accumulation in each group. After staining, lipids can be extracted with 60% isopropanol, and the absorbance of the dye at 510nm can be measured for quantitative comparison. Under the same 1μmol / L DHT stimulation condition, the oil droplet content of the test sample group decreased compared with the negative control group, indicating that the sample has the ability to inhibit oil secretion at this test concentration. The inhibition rate calculation formula is: inhibition rate (%) = (oil droplet content in the negative control group - oil droplet content in the test sample group) / oil droplet content in the negative control group × 100%. The results are shown in Table 2.

[0069] Table 2 Relative content and inhibition rate of oil droplets in sebaceous gland cells

[0070]

[0071]

[0072] Result analysis:

[0073] Compared with the negative control group, the oil droplet content of components 1-4 showed a decrease. The inhibition rates of oil secretion of human sebaceous gland cells of components 1-4 at a concentration of 10wt% were 30.11%, 31.26%, 33.15% and 30.74%, respectively, indicating that the oil-controlling and skin-astringing composition provided by the present invention has excellent performance in inhibiting the oil secretion of human sebaceous gland cells and has excellent deep oil control effect on the skin.

[0074] Preparation of Example:

[0075] Weigh the raw materials according to Table 3;

[0076] S1. The thickener, humectant and deionized water were stirred and mixed, heated to 90°C, homogenized at a speed of 1200 rpm for 10 min, and kept warm for use after the homogenization to obtain component A;

[0077] S2. The preservative, chelating agent and emulsifier were stirred and mixed with deionized water, heated to 60°C, homogenized at 800 rpm for 5 min, and kept warm for use after homogenization to obtain component B;

[0078] S3. Component A is cooled to 60°C, component B is added at a speed of 500 rpm and stirred for 10 minutes, then the temperature is cooled to below 45°C, the oil-controlling and skin-smoothing composition is added at a speed of 500 rpm and stirred for 10 minutes, and finally a pH adjuster is added to adjust the pH to 6, stirring is stopped, and the material is discharged to obtain an essence.

[0079] Table 3 Mass percentage of raw materials in the examples

[0080]

[0081] Note: “-” in the table means no addition.

[0082] Performance test of the embodiment:

[0083] 1. Oil control effect test

[0084] 1.1 Instant oil control effect test

[0085] The above-mentioned Examples 1-3 were used as the test group, and the blank control example was used as the blank control group, and the instant oil control effect test was performed on the arm skin of the skin test subjects.

[0086] Test method: 15 male subjects were selected and divided into 3 groups, 5 in each group. Two 4cm×4cm test areas were selected from the corresponding positions on the inner side of both arms of the 15 skin subjects, marked as A and B respectively. A latex finger cot was used to apply (1.0±0.1) mg / cm 2 Squalane, and 3 minutes after application, apply (2.0±0.1)mg / cm2 on area A 2 As a blank control, apply (2.0±0.1) mg / cm2 on area B. 2 After applying the sample, the skin test area was tested using the skin test system of the SkinLab Combo skin detector provided by the Danish company Cortex. The instrument probe was used to test the test area and give the oiliness value. The average value of the results of all subjects (retaining one decimal place) was taken as the final result of the sample. The specific oiliness value data of each group are shown in Table 4.

[0087] Table 4 Oil gloss value test results

[0088]

[0089]

[0090] 1.2 Test of long-lasting oil control effect

[0091] The above-prepared Examples 1-3 and the blank control example were used as test samples, wherein Examples 1-3 were used as the test group, and the blank control example was used as the blank control group, and the forehead skin of the skin test subjects was tested for the long-lasting oil control effect. The sebum test system of the SkinLab Combo skin detector provided by the Danish Cortex company was still used to test the oil content.

[0092] Test method: 20 male subjects were selected. There was no significant difference in the facial oil level of these 20 subjects. They were divided into 4 groups, with 5 people in each group. The method of use was that the subjects took 2.0±0.5g of the test sample and evenly applied it on the face in the form of smear after washing their faces with the standard cleanser in the morning and evening; the subjects performed a facial oil content test before starting to use the test sample, and the initial value was recorded as W0; the facial oil content test was performed after using the test sample for 2 weeks, recorded as W2; the facial oil content test was performed after using the test sample for 4 weeks, recorded as W4; the average value of each group of data was taken (keeping two decimal places), and the specific test results are shown in Table 5.

[0093] Table 5 Grease content test results

[0094]

[0095] 1.3 Facial pore change detection

[0096] The above-prepared Examples 1-3 and the blank control example were used as test samples, wherein Examples 1-3 were used as the test group, and the blank control example was used as the blank control group, and the facial skin of the skin test subjects was subjected to pore feature counting detection. The facial image acquisition device VISIA provided by Canfield Company of the United States was used to detect the pore feature counting. The pore feature count is the number of pores obtained by VISIA statistics, and the parameter is obtained by VISIA's own software analysis.

[0097] Experimental method: 20 male subjects were selected. There was no significant difference in the facial pore feature count levels of these 20 subjects. They were divided into 4 groups, with 5 people in each group. The method of use was that the subjects took 2.0±0.5g of the test sample and evenly applied it on the face in the form of smear after washing their faces with the standard facial cleanser in the morning and evening; the subjects performed a pore feature count test before starting to use the test sample, recorded as W0; the pore feature count test was performed after using the test sample for 2 weeks, recorded as W2; the pore feature count test was performed after using the test sample for 4 weeks, recorded as W4; the average value of each group of data was taken (keeping one decimal place), and the specific results are shown in Table 6 below.

[0098] Table 6 Changes in pore feature counts

[0099]

[0100] 1.4 Results Analysis

[0101] According to the test results, the leave-on skin topical preparation provided by the present invention has excellent short-term oil control, long-lasting oil control and skin astringent effects. Compared with the blank control group, the test group has obvious effects on oil control and reduction of enlarged pores. At the same time, the oil control and skin astringent performance of the skin topical preparation is enhanced with the increase of the concentration of the composition, showing concentration dependence, indicating that the composition is a key ingredient for oil control and skin astringency in the overall formula.

[0102] Although the specific embodiments of the present invention are described above, it should be understood by those skilled in the art that this is only for illustration and the protection scope of the present invention is defined by the appended claims. Those skilled in the art may make various changes or modifications to these embodiments without departing from the principles and essence of the present invention, but these changes and modifications all fall within the protection scope of the present invention.

Claims

1. An oil-controlling and skin-smoothing composition, characterized in that: The composition comprises the following components in parts by mass:

2. The oil-controlling skin-smoothing composition according to claim 1, characterized in that: The composition comprises the following components in parts by mass:

3. The oil-controlling skin-smoothing composition according to claim 2, characterized in that: The composition comprises the following components in parts by mass:

4. The oil-controlling skin-smoothing composition according to claim 3, characterized in that: The composition comprises the following components in parts by mass:

5. Use of the oil-controlling and skin-smoothing composition according to any one of claims 1 to 4 in the preparation of a skin care product having oil-controlling, acne-removing, anti-oxidation, antibacterial, anti-inflammatory and / or soothing effects.

6. A leave-on skin topical preparation with oil-controlling and skin-smoothing effects, characterized in that: The leave-on skin topical preparation comprises the oil-controlling and skin-smoothing composition according to any one of claims 1 to 4; The amount of the oil-controlling and skin-astringing composition added to the leave-on skin external preparation is 5-10 wt %.

7. The leave-on skin external preparation according to claim 6, characterized in that: The leave-on skin external preparation further comprises a thickener, a moisturizer, a preservative, a chelating agent, an emulsifier, a pH adjuster, and a solvent.

8. The leave-on skin external preparation according to claim 7, characterized in that: The thickener is selected from at least one of acrylic acid (esters) / C10-30 alkyl acrylate cross-linked polymer and carbomer; The moisturizing agent is selected from at least one of glycerin, allantoin, 1,3-propylene glycol, butylene glycol, and 1,2-hexanediol; The preservative is p-hydroxyacetophenone; The chelating agent is disodium EDTA; The emulsifier is PPG-5-cetoeth-20; The pH regulator is arginine; The solvent is deionized water.

9. The method for preparing the leave-on skin external preparation according to claim 8, characterized in that: The preparation method comprises the following steps: S1. The thickener, humectant and deionized water are stirred and mixed, heated to 80-90°C, homogenized at a speed of 1000-1500rpm for 5-10min, and kept warm for standby after homogenization to obtain component A; S2. The preservative, chelating agent and emulsifier are stirred and mixed with deionized water, heated to 50-60°C, homogenized at a speed of 800-1000rpm for 3-5min, and kept warm for standby after the homogenization to obtain component B; S3. Component A is cooled to 50-60°C, component B is added at a rotation speed of 500-600rpm, and the mixture is stirred and mixed for 5-10min. Then, the temperature is cooled to below 45°C, the oil-controlling and skin-smoothing composition is added at a rotation speed of 500-600rpm, and the mixture is stirred and mixed for 5-10min. Finally, a pH adjuster is added to adjust the pH to 5.5-6.5, stirring is stopped, and the material is discharged to obtain a leave-on skin topical preparation.

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