294 results about "Ginkgolic acid" patented technology

The invention discloses a preparation method of detoxified gingko tea. The preparation method comprises the following steps: 1) preparing a fermenting agent, namely, separating eurotium cristatum from Fuzhuan dark tea and preparing a solid pure strain fermenting agent; 2) pretreating gingko leaves, namely, cleaning the gingko leaves, removing impurities, slitting and carrying out fixation, cooling and twisting after fixation so as to obtain raw tea; 3) blending and carrying out pile fermentation, namely, blending the raw tea of which the impurities are removed with dark raw tea, and carrying out pile fermentation on the blended tea leaves after primary steaming; 4) inoculating, namely, adding the solid pure strain fermenting agent into the tea leaves after pile fermentation and secondarily steaming and inoculating; 5) briquetting and fermenting: compressing the inoculated tea leaves into tea bricks, and putting the tea bricks into a fermentation chamber for fermenting; and 6) dehydrating and packaging: dehydrating the fermented tea bricks and dehydrating, and packaging the dehydrated tea bricks directly. The method not only can be used for effectively retaining active substances benefiting to human bodies, but also can be used for reducing the content of ginkgolic acid; the detoxified gingko tea is safe and reliable in quality, and is suitable for large-scale production and popularization.

The invention relates to a gingko leaf composition and a preparation method thereof. The composition comprises the following components: 24 to 45 percent of total flavonol glycoside, 4 to 18 percent of ginkgolide, 2 to 6 percent of bilobalide, and less than 5 ppm of ginkgoic acid. The invention provides a simple, feasible and reliable method, wherein total flavonoid and terpene lactones in a certain proportion can be obtained simultaneously in the extraction process the yield is high, purity of effective components is high and fewer impurities exist, and harmful substances such as alkylphenolic acid can be effectively removed.

The invention discloses a method of preparing a plurality of activation materials from ginkgo leaves, and belongs to a manufacturing method of chemical substances or a manufacturing method of drugs. Strong polarity macroporous adsorption resin or polarity macroporous adsorption resin is adopted for separation, and is adopted to solve the problems that the amount of ginkgolic acid is high when the prior ginkgo extract is prepared, and solve the problems that a large amount of organic solvent is consumed when the prior ginkgo active substances are extracted and prepared, and accordingly the environment is polluted, or that a complex separation technology can not enlarge production, or that extraction efficiency is not high, and use ratio of raw materials is low and the like. The method of preparing the plurality of activation materials from the ginkgo leaves can simultaneously obtain a plurality of active substances such as ginkgo polysaccharide, ginkgol biloba extracts, ginkgol biloba general flavone, ginkgol biloba total lactones, gbilobalide A monosome, gbilobalide B monosome, gbilobalide C monosome and the like. The technical scheme is that the method comprises a raw material pretreatment step, an extraction step, a polysaccharide precipitation step, a macroporous resin separation step, a silica gel dry-column separation step, a reversed phase chromatography purification step, a crystallization step and the like, saves production cost, and improves work efficiency. The process of the method is simple and convenient to conduct. The method can be easily enlarged to the commercial process, and meanwhile greatly increase use ratio of raw materials.

The present invention relates to an extraction technique of natural plant active component. In the concrete, it relates to a preparation process of ginkgo extract with low ginkgolic acid content. In the ginkgo extract obtained by adopting the invented preparation method the ginkgo total flavone content is 28-40%, bilobalide content is 6-14% and ginkgolic acid content is less than 5ppm. Its preparation method includes the steps of extraction, separation, purification, concentration and drying.

The present invention discloses a method for removing ginkgolic acid from ginkgo leaf extract by orgnaic solvent extraction process, and said process mainly includes: extracting and drying. In which the refining procedure includes: adding methyl alcohol or ethyl alcohol to pulverized ginkgo leaf extract, stirring, dissolving, standing still, filtering, reduced pressure concentrating and drying; and its extracting procedure includes: stirring the pulverzed ginkgo leaf extract with ethyl alcohol or acetone, dissolving and placing said material into extract, adding paraffins material, uniformly mixing them, standing still and demixing, removing upper layer, repeatedly operating lower layer for several times, drying.

An insecticide for killing oncomelania is prepared from the external seed vessel of gingko through reflux extracting in petroether, alcohol, or ether, vacuum concentrating, separating and purifying by macroreticular resin or chromatography with silicon gel column to obtain the ginkgolic acid mixture, and dissolving it in the aqueous solution of cosolvent.

The invention relates to a high-purity folium ginkgo composition, a preparation including same and a preparation method thereof. The composition comprises ginkgo flavonoids refined content and ginkgo total lactone refined content according to the weight ratio of (3-9):1. The content of total ginkgolic acid in the composition is not beyond 3%. Flavonoids and total lactone have high purity and less impurity. Injection prepared from the composition is light coloured, safe and effective and has proper active ingredient proportion and steady quality.

The invention discloses a ginkgo toxicity removal method based on a physical and enzymatic combination method. Through screening of substantial experiments, toxic component degrading enzymes of ginkgoes are used for bioconversion at the optimal temperature under optimal moisture conditions, toxic component 4-0-methyl pyridoxine and cyanogentic glycoside type components of the ginkgoes are removed efficiently, allergic protein in the ginkgoes are denatured and inactivated through high-temperature treatment, degraded products of the cyanogentic glycoside components are evaporated and removed, toxic component ginkgo acid is removed, moisture of the ginkgoes is reduced to some extent according to distribution of the ginkgo acid in the ginkgoes, and germ positions with the most gingko acid are removed manually or mechanically. The ginkgo toxicity removal method has the advantages of reasonableness in overall process design, high detoxification efficiency, environment protection, safety, non-existence of residual solvent, convenience in operation, low cost and capabilities of overcoming defects in the prior art and achieving industrial production.

The invention discloses a removal technique of ginkgolic acid in a ginkgo leaf extract, which utilizes ginkgo leaf extract which passes through a macroreticular resinous to be raw material, and the process comprises firstly utilizing polyamide which is pre-processed to be a removing agent of the ginkgolic acid, and then changing superpositions of resin pillar with pre-processing strong alkalinity macroporous ion to remove ginkgolic acid. The process has simple technique, stability and low cost, which can effectively remove ginkgolic acid in ginkgo leaf extract, and can not affect yield rates of ginkgo biloba flavonoids and ginkgolide.

The present invention discloses a kind of ginkgo leaf extract, injection containing the said extract and its preparation process. The ginkgo leaf extract contains flavonol glycoside 18-40 wt%, bilobalide 2.5-25 wt%, and heavy metals less than 9.4 ppm, with the bilobalide comprising bilobalide A, bilobalide B and bilobalide C in the weight proportion of 7-27 to 3-20 to 4-40. The ginkgo leaf extract with high effective component content and high purity, and the prepared medicine preparation, especially injection, has obvious curative effect on cardiac and cerebral vascular diseases, clinical use safety and no side effect.

The invention discloses low-acid ginkgo flavone and an extraction method thereof. The invention is characterized in that the content of the ginkgo flavonoid glycosides of the low-acid ginkgo flavone is over 27 percent, the content of ginkgolides is over 6.5 percent, and the content of ginkgoic acid is lower than 1PPM. The low-acid ginkgo flavone is extracted according to the following method: crushing folium ginkgo, marinating the crushed folium ginkgo in ethanol; recollecting the ethanol used for marinating the crushed folium ginkgo; removing impurities from the marinated folium ginkgo; adding resin to absorb the ginkgoic acid for the first time; eluting with ethanol so as to obtain an eluting solution; adding resin to absorb the ginkgoic acid for the second time; eluting with ethanol so as to obtain an eluting solution; recollecting the ethanol; obtaining the low-acid ginkgo flavone after the drying. Compared with other extraction methods, the extraction efficiency of the low-acid ginkgo flavone extracted through the adoption of the method is improved by 0.2 percent, the content of the ginkgo flavonoid glycosides is improved by 3 percent, the content of the ginkgolides is improved by 0.5 percent and the content of ginkgoic acid is lower than 1PPM.

A gingko leaf extract used for preparing liquid injection or freeze-dried powder injection is prepared from gingko leaves through breaking, thermal reflux extracting with the aqueous solution of alcohol, vacuum volatilizing of solvent, depositing in ammonia water to remove polyphenol, extracting with n-hexane to remove ginkgolic acid, adsorbing by macroreticular resin, separating, refining, ultrafiltration and spray drying.

The invention discloses a gingkgo active ingredient extraction and separation method and use of extracted active matters in medicines for treating cardiovascular and cerebrovascular diseases. The method comprises the following steps: 1) grinding gingkgo leaves, and extracting gingkgo leaf active ingredients, namely flavone and terpene lactone compounds; 2) removing ginkgolic acids; separating gingkgo flavones from terpene lactones; and 4) purifying the terpene lactones by high performance liquid chromatography. The tests of effects of high-purity gingkgo terpene lactone medicines on the heart function of adult and old rats at organ, body and cell levels prove gingkgo terpene lactones have protective effects on the heart of Sprague Dawley (SD) adult and old rats. The method has the advantage that four terpene lactones with high purity of over 98 percent are prepared by high performance liquid chromatography. The ginkgoic acid content in the gingkgo leaf extract is controlled to be within 1ppm, the separation of flavones from terpene lactones is realized, the content of gingkgo flavones after separation reaches over 55 percent, and the gingkgo terpene lactone content reaches over 25 percent. All of the three indexes reach national standards.

The invention discloses a separation technique for multiple components in ginkgo leaves. Ginkgo leaves are used as a raw material. By combination of ultrasonic extraction and supercritical CO2 extraction-distillation, separation of multiple effective components is realized. The separation technique provided by the invention comprises the following steps: firstly, extracting the ginkgo leaves by ultrasonication in ethanol solutions with different concentrations for two times, and carrying out the following processes on filter liquor and filter residue obtained by filtration: (1) carrying out supercritical CO2 extraction and separation on the filter residue to obtain polyprenol compounds, and separating raffinate by water extraction and alcohol precipitation to obtain ginkgo polysaccharides; and (2) carrying out ultrafiltration and concentration on the filter liquor, and firstly separating to obtain ginkgolic acids by the supercritical CO2 extraction at a proper temperature and a properpressure; and changing extraction conditions, starting a rectifying column, heating by the temperature variation in the axial direction in four segments, and continuously extracting and separating toobtain ginkgo terpene lactones and flavonoid compounds. The separation technique provided by the invention has the advantages that active ingredients are difficult to decompose or destroy, harmful chemical reagent residues do not exist, the extraction efficiency is high, the separation effect is good and the like, solves the problem that the content of poisonous phenolic acid in ginkgo leaf extract exceeds the standard, and is an environment-friendly, economic and industrialization-suitable technique.

The invention discloses a method for reducing the content of ginkgolic acid in ginkgo seeds, and belongs to the technical field of food processing. According to the method disclosed by the invention, simple heat treatment and alcohol soaking treatment are combined, so that the content of the ginkgolic acid in the ginkgo seeds can be notably reduced, and the safety of the ginkgo seeds is greatly improved. Besides, the method disclosed by the invention is simple to operate and low in cost, and has good popularization potential.

The invention discloses a method for removing ginkgolic acid from a ginkgo biloba extract. The method comprises the following steps of: extracting, performing column chromatography, and drying, wherein the column chromatography process comprises the following steps of: regulating the relative density of a filtrate obtained by extracting and filtering by using water to be 1.05 to 110 to obtain an extracting solution, allowing the extracting solution to pass through a macroporous adsorption resin exchange column in a ratio of adding 1.5 to 3 milliliters of extracting solution into each gram of resin, washing by using the water, eluting by using 60 to 80 mass percent ethanol solution, and collecting 60 to 80 mass percent ethanol eluent, wherein the macroporous adsorption resin is an LSA series macroporous adsorption resin. According to the ginkgo biloba extract obtained by the method, the content of total flavonol glycosides is more than 35 percent, the content of total terpene lactones is more than 12 percent, and the content of total ginkgolic acid is less than 1 ppm.

The invention relates to the use of ginkgoic acid in preparation of biological pesticide for killing Oncomelania snail and preventing schistosomiasis, wherein experiment result shows that, the ginkgolic acid can effectively inhibit key enzyme and de-coupling reaction to mitochondria oxidation phosphorylation in mollusk energy supply biochemical metabolism, effectively inhibit tyrosinase during insect exuviation procedure and prostaglandin synthetase during breeding process, thus can eradicate unwanted mollusk harming agronomic crops.

The invention belongs to the technical field of biopesticides, discloses application of ginkgoic acid and ginkgol compounds to killing agriculturally harmful mollusks, and discloses novel application of the ginkgoic acid and ginkgol to preparation of the biopesticides. The invention shows that the ginkgoic acid and ginkgol compounds can effectively inhibit the synthesis of glycogen and protein inmollusk ampullaria gigas, can simultaneously inhibit the activities of ChE, SDH and MDH in the cephalopodium and the liver of the ampullaria gigas, interfere a NADH respiratory chain and cause energymetabolic dysfunction so as to cause cell energy supply deficiency and unattainability of normal synthesis and secretion functions, and promote the lack of important proteins and enzymes in the body of the ampullaria gigas so as to cause the death of the ampullaria gigas. The ginkgoic acid and the ginkgol are readily degradable in the natural environment, and have small influence on the environment; and an acute toxicity test proves that the ginkgoic acid and the ginkgol are proved to be slightly toxic pesticides, can be developed into novel biopesticides, and can be used for preventing the ampullaria gigas for rice, vegetables and fruits.

The invention relates to an application of ginkgolic acid in preparation of an external preparation for treating dermatopathy. The ginkgolic acid comprises ginkgoic acid, ginkgol, ginkgo diphenol, and the like, and can be obtained from extracts of leaves, fruit, exopleura, and rhizome of ginkgo by a chemical separation method. The dermatopathy is infectious, inflammatory, allergic or anaphylactic dermatopathy. External preparations of ginkgolic acid-containing medicaments, cosmetics, or disinfectants for treating dermatopathy comprises 0.001-80% (by weight) of ginkgolic acid, and pharmaceutically common external preparation substrates or acceptable carriers; the preparation has any pharmaceutical dosage form. Pharmacological experimental research shows that the preparation of the invention has significant effect of anti-infection of skin resident pathogens, anti-inflammation, antianaphylaxis, anti-allergy, analgesia, itching relief, and wound heal promotion. Clinical trials show that the preparation of the invention has significant treatment effect on various dermatopathy, has an effective rate of up to 100%, is safe and has no side effect.

The invention relates to an agricultural insecticide, particularly relates to gingkolic-acid microemulsion for botanical insecticide and bactericide. The gingkolic-acid microemulsion is made of gingkolic-acid stock solution, solvent, cosurfactant and surfactant mixed and agitated evenly, and then added with tap-water and then agitated again. The invention has the advantages of reasonable formulation, stable physical and chemical performances, easy operation of production, remarkable effect of insect disinfestations; the insecticide can be used to prevent and kill cotton aphid, rice borer, Prodenialitura Fabr. and other major plant diseases and insects, and has eminent effect in inhibiting the sprout of the spores of potato late blight.

The invention discloses application of ginkgolic acid in killing of a blue-green algae, application of an alcohol extract of ginkgo biloba sarcotesta in killing of the blue-green algae, a composition and application of the composition in killing of the blue-green algae. It is observed that the ginkgolic acid and a preparation containing the ginkgolic acid have strong activity of killing the blue-green algae from the embodiment of the invention. In addition, the ginkgolic acid is plant source algicide, is taken from the natural world and applied to the natural world, is low in toxicity and residue, and is an algicide which is friendly to the environment, and simple in using method; and the drug resistance is not easily generated.

The invention relates to a method for negative-pressure boiling extraction and preparation of a low-acid ginkgo extract from ginkgo leaves. According to the method, crushed fresh ginkgo leaves or dry ginkgo leaves are used as a raw material, hydrous ethanol is used as an extraction solvent, negative-pressure boiling extraction is carried out, then a liquid extract is filtered and then pretreated by using quartz sand and microporous centrifugal filtration, a filtrate is separated and purified by using a ceramic membrane and an ultrafiltration membrane, a solution passing through the ultrafiltration membrane is concentrated by using a nanofiltration membrane, then ethanol is recovered, a concentrate is subjected to purification with a macroporous resin and phenolic acid removal with a deacidification resin, and negative-pressure microwave spray drying or vacuum freeze drying is carried out so as to obtain the low-acid ginkgo extract. The low-acid ginkgo extract prepared by using the method has a low acid value, high purity, ginkgetin content of more than 28%, lactone content of more than 8% and ginkgolic acid of no greater than 1 ppm, and the method may become a novel technology for industrial production of a low-acid ginkgo extract product.

The invention discloses a method for reducing toxic substances in ginkgo biloba, belonging to the technical field of food processing. According to the method, the ginkgo biloba is firstly frozen and is then unfrozen; the fresh ginkgo biloba has very high water content, so that the structure of the ginkgo biloba changes after the ginkgo biloba is frozen and unfrozen in an environment with the temperature of -18 to -50 DEG C, and the operation of reducing the toxic substances by heating in the later period is facilitated. After that, microwave heat-treatment can enable the temperature of the ginkgo biloba to rise rapidly, so that the shell of the ginkgo biloba is broken, and a certain amount of cyanide and ginkgolic acid can be effectively removed. After the shell of the ginkgo biloba is broken, infrared heat treatment is subsequently carried out on the ginkgo biloba, and the ginkgo biloba is kept at high temperature, so that the content of the ginkgolic acid is greatly reduced.

The invention relates to a high-purity ginkgo flavone and a composition thereof. The high-purity ginkgo flavone is light brownish yellow, and tastes slightly bitter; the content of ginkgo flavone is greater than or equal to 50%, the peak ratio of quercetin to kaempferol is 0.8-1.2, the peak ratio of isorhamnetin to quercetin is greater than or equal to 0.15, the concentration of ginkgoic acid is smaller than 5ppm, and the content of residue on ignition is smaller than 0.8%. The water content is smaller than 5.0%. The method for preparing the high-purity ginkgo flavone comprises the following steps: extracting by heating ginkgo leaves, which are used as the raw material, under reflux with a 20-80 wt% ethanol solution; concentrating the extracting solution to recover ethanol; separating by passing through macroporous resin columns; carrying out ethanol desorption; diluting the product by adding water; adsorbing by passing the product through a gel-type hydrogen bond adsorption resin which is mainly based on hydrogen bond adsorption, and desorbing; and collecting the effluent liquid and the desorption liquid, concentrating and drying. The preparation technique is simple and reliable, has the advantage of low cost, and does not use toxic organic solvents in the process, thereby being environment-friendly. The high-purity ginkgo flavone can be made into a composition as an effective constituent, and the composition can be used as various preparations (capsules, tablets, oral liquids, granules, powder, injections and the like) of ginkgo flavone medicines or health products.

The invention relates to a method for removing ginkgolic acid in gingko biloba seeds. The method is characterized by comprising the following steps: the gingko biloba seeds peeled are placed into an opened container, then water is added, an alkali solution is added into another opened container, the two opened containers are placed in a pressure resistant distilling kettle for heat treatment, the heating and digesting temperature ranges from 105 DEG C to 200 DEG C, the processing time lasts for 20 to 60 min, and the concentration of the alkali solution is 10 wt%, in saturation. According to the method provided by the invention, alkali and the gingko biloba seeds (ginkgo) are separately placed into a pressure tight case at an interval, heating is performed, the gingko biloba seeds are stewed, alkali is used for absorbing carbon dioxide produced during the ginkgolic acid heating and the decarboxylating process, the removing effect of the ginkgolic acid in the gingko biloba seeds is enhanced, and ginkgolic acid residue reduction and the processing time shortening are facilitated.

The invention relates to a preparing process for folium ginkgo purifying freeze dried powder, characterized that: ginkgoic acid as an injurious ingredient can be effectively removed from the folium ginkgo by a supercritical CO2 extraction technology replacing traditional alcohol extraction; the product has stronger efficacy by the fact that it is much easer to preserve bioactive component of the folium ginkgo by extracting in low temperature of 35-40 DEG C. than that in high temperature of 65-75 DEG C; bioactive damage of the product during drying is close to zero by vacuum freeze drying process in temperature of -40 DEG C. rather than traditional spray drying process in 150 DEG C; due to a separation and purification process of biomembrane method and chromatography, purity of the product is greatly improved compared with the traditional product, the content of ginkgo flavone and lactone as active ingredient is more than 50%, and injurious ingredient is further reduced, so that the content of the ginkgoic acid is less than 5ppm.

The invention discloses a preparation method of total terpene lactones with low phenolic acid, which uses ginkgo biloba extract as a raw material, uses ethyl acetate backflow to extract and uses amide resin to purify and deacidify. The preparation method takes the ginkgo biloba extract as the raw material and water saturated ethyl acetate as a solvent; at the weight measurement ratio of extractives to the solvent of 1:1-15, the extractive and solvent are extracted for 0.5-5 hours at the temperature of 20-90 DEG C and filtered; filtrate is concentrated to obtain extractum; after the extractum is adsorbed by polyamide resin, the extractum is eluted by 10-50% of lower alcohol solution; fraction is collected, concerted and dried; and the fraction is smashed to obtain dry powder in which the total terpene lactone content is above 50% and ginkgoic acid is lowered than 1 ppm. The preparation process of the invention has short extract time, low extract temperature, high extract efficiency, small solvent amount, solvent and energy saveing and lowered production cost; the deacidifying process method is convenient to operate and can lower the ginkgolic acid below 1 ppm; and the technical method provided by the invention can effectively remove tannin and pigment in extractives.

The invention provides a ginkgoic acid preparation method using macroporous adsorption resin, comprising the following steps: absorbing ginkgoic acid from the feed liquid containing ginkgoic acid until being saturated using the macroporous adsorption resin; using water to wash out the residual feed liquid which is not absorbed by the resin; washing the impurities with lower resin absorption compared with the ginkgoic acid using the non-alkaline hydrophilic organic solvent aqueous solution; eluting ginkgoic acid constituents using the non-acidity hydrophilic organic solvent aqueous solution; adjusting the p H value of the eluent; decompression recovering the solvent and vacuum concentrating and drying the eluent to produce the finished products of the ginkgoic acid. The preparation method has features of novel design, simple and easily executed technology, best quality, low production cost, and can be used in further separation and purification of the feed liquid containing ginkgoic acid from separate sources with larger generalization.

The invention discloses a method for removing and enriching ginkgolic acid from ginkgo biloba extract. The method comprises the following steps: carrying out column filling by taking a molecularly imprinted polymer of ginkgolic acid as column chromatography packing; then filling the column with a ginkgo biloba extracting solution; leaching the column with a mixed solution of cyclohexane and ethyl alcohol in a volume ratio of 10 to 1; finally, eluting the chromatographic column with alcohol so as to obtain an effluent, namely ginkgolic acid, wherein the alcohol can be of ethyl alcohol, isopropanol, n-propyl alcohol or n-butyl alcohol. A molecular imprinting technique is applied to the removal of ginkgolic acid during ginkgo biloba processing for the first time, so that compared with the traditional ginkgolic acid removal method, according to the method disclosed by the invention, ginkgolic acid with high purity is prepared while ginkgolic acid is removed by virtue of the directional enrichment effect of the molecularly imprinted-solid phase extraction column, thereby providing the source of a raw material for clinical pharmacological research on ginkgolic acid.

The invention relates to a water-soluble ginkgo leaf extract and its preparation technology, The method of the invention comprises: taking common ginkgo biloba as a raw material, mixing with acetone and extracting, concentrating, diluting by adding water, cryoprecipitating, filtering, performing a gradient elution to a filtrate through macroporous adsorption resin, carrying out an alcohol precipitation by ethanol with high purity, water extraction and cryoprecipitating, concentrating, drying to obtain the water-soluble ginkgo leaf extract, wherein the content of total flavonol glycoside is greater than or equal to 27%; the content of lactone content is 9%-12%, wherein the content of Terpene Lactones A is greater than or equal to 2.1%, the content of Terpene Lactones B is greater than or equal to 0.9%, the content of Terpene Lactones C is greater than or equal to 1.2%, and the lactone ratio of GA:GB:GC is close to 7:3:4; the water-solubility is greater than and equal to 10%; the ginkgolic acid is less than and equal to 1ppm, which can be taken as a raw material used for injection. The invention has the advantages of simple production technology, high and stable product quality, high finished product yield, low cost and good economic benefit.