Preparation method of detoxified gingko tea
A production method and technology of ginkgo tea, applied in the field of tea processing, can solve the problems of high price of ginkgo leaf extract, difficulty in exerting the efficacy of ginkgo tea, inability to promote large-scale, etc., and achieve shortened production cycle, shortened flowering time, and easy scale. The effect of chemical production
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preparation example Construction
[0027] 1) Preparation of leavening agent:
[0028] ① Firstly, isolate S. coronoidis from Fuzhuan tea, purify and identify it, store it on a low-temperature slant, and use PDA to expand and cultivate it for later use;
[0029] 2. prepare starter culture medium then, starter culture medium comprises the tea that tea-water ratio is 1 / 40, every 100L tea water adds bran 100kg, glucose 6kg, yeast extract powder 4kg, NaCl 0.8kg; Described starter culture medium is at 121 Sterilize for 30 minutes at a temperature of ℃ or at a pressure of 0.1-0.15 MPa;
[0030] ③The expanded and cultivated S. coronoidis prepared at a concentration of 2×10 7 ~9×10 7 Bacteria solution per mL, inoculated on the above-mentioned starter culture medium, the inoculum amount was 20ml / kg relative to the above-mentioned starter culture medium, the culture time was 8 days, the culture temperature was 26-28°C for the first three days, and 26-28°C for the next five days. 24-26°C, when the golden-yellow closed cy...
Embodiment 1
[0048] A preparation method of detoxification ginkgo tea, comprising the following steps:
[0049] 1) Preparation of leavening agent:
[0050] ① Firstly, isolate S. coronoidis from Fuzhuan tea, purify and identify it, store it on a low-temperature slant, and use PDA to expand and cultivate it for later use;
[0051] 2. prepare starter culture medium then, starter culture medium comprises the tea that tea-water ratio is 1 / 40, every 100L tea water adds bran 100kg, glucose 6kg, yeast extract powder 4kg, NaCl 0.8kg; Described starter culture medium is at 121 Sterilize for 30 minutes at a temperature of ℃ or a pressure of 0.1 MPa;
[0052] ③The expanded and cultivated S. coronoidis prepared at a concentration of 2×10 7 ~9×10 7 Bacteria solution per mL, inoculated on the above-mentioned starter medium, the inoculum amount is 20ml / kg relative to the above-mentioned starter medium, the culture time is 8 days, the culture temperature is 26°C for the first three days, and 24°C for th...
Embodiment 2
[0060] A preparation method of detoxification ginkgo tea, comprising the following steps:
[0061] 1) Preparation of leavening agent:
[0062] ① Firstly, isolate S. coronoidis from Fuzhuan tea, purify and identify it, store it on a low-temperature slant, and use PDA to expand and cultivate it for later use;
[0063] 2. prepare starter culture medium then, starter culture medium comprises the tea that tea-water ratio is 1 / 40, every 100L tea water adds bran 100kg, glucose 6kg, yeast extract powder 4kg, NaCl 0.8kg; Described starter culture medium is at 121 Sterilize for 30 minutes at a temperature of ℃ or a pressure of 0.12 MPa;
[0064] ③The expanded and cultivated S. coronoidis prepared at a concentration of 2×10 7 ~9×10 7 Bacteria liquid per mL, inoculated on the above-mentioned starter medium, the inoculum amount is 20ml / kg relative to the above-mentioned starter medium, the culture time is 8 days, the culture temperature is 27°C for the first three days, and 25°C for the...
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