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Separation technology for multiple components in ginkgo leaf

A separation technology, the technology of ginkgo biloba, which is applied in the field of separation of multi-components of ginkgo biloba, can solve the problem of excessive toxic phenolic acid in ginkgo leaf extract, achieve high purity, solve the problem of excessive toxic phenolic acid, and good separation effect

Active Publication Date: 2012-07-25
福建华尔康生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The invention has the advantages of not being easy to be decomposed and destroyed, no harmful chemical reagent residue, high extraction efficiency, good separation effect, etc., and at the same time solves the problem of excessive toxic phenolic acids in the extract of ginkgo biloba leaves. It is an environmental protection, economical and industrializable Implemented technology

Method used

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  • Separation technology for multiple components in ginkgo leaf
  • Separation technology for multiple components in ginkgo leaf

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Embodiment 1: Ginkgo biloba component separation

[0022] Raw materials: 3-year-old ginkgo leaves picked in June.

[0023] step:

[0024] (1) Remove impurities from the ginkgo leaves, dry until the water content is ≤5%, pulverize, and weigh 3000g of ginkgo leaves of 10-24 mesh.

[0025] (2) Add 10 times the amount of 85% ethanol to impregnate for 60 minutes, extract for 30 minutes under the condition of ultrasonic power 2800W, 42°C, and then filter; the filter residue is then ultrasonically extracted with 10 times the amount of 70% ethanol, the conditions are the same as above, filtered and combined The filtrate was used twice.

[0026] (3) The filter residue of step (2) is subjected to supercritical CO 2 Extraction, the conditions are: extraction kettle pressure 26MPa, extraction temperature 52°C; separation temperature 40°C, first-stage separation kettle pressure 9MPa, second-stage separation kettle pressure 6MPa, CO 2 Flow rate 2.2L / min (food grade, purity ≥ 99.5...

Embodiment 2

[0031] Embodiment 2: Ginkgo biloba component separation result

[0032] Each component obtained in Example 1 was detected and analyzed, and its compound quality, purity, and extraction rate are shown in Table 1.

[0033] Table 1 Separation results of Ginkgo biloba components

[0034]

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PUM

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Abstract

The invention discloses a separation technique for multiple components in ginkgo leaves. Ginkgo leaves are used as a raw material. By combination of ultrasonic extraction and supercritical CO2 extraction-distillation, separation of multiple effective components is realized. The separation technique provided by the invention comprises the following steps: firstly, extracting the ginkgo leaves by ultrasonication in ethanol solutions with different concentrations for two times, and carrying out the following processes on filter liquor and filter residue obtained by filtration: (1) carrying out supercritical CO2 extraction and separation on the filter residue to obtain polyprenol compounds, and separating raffinate by water extraction and alcohol precipitation to obtain ginkgo polysaccharides; and (2) carrying out ultrafiltration and concentration on the filter liquor, and firstly separating to obtain ginkgolic acids by the supercritical CO2 extraction at a proper temperature and a properpressure; and changing extraction conditions, starting a rectifying column, heating by the temperature variation in the axial direction in four segments, and continuously extracting and separating toobtain ginkgo terpene lactones and flavonoid compounds. The separation technique provided by the invention has the advantages that active ingredients are difficult to decompose or destroy, harmful chemical reagent residues do not exist, the extraction efficiency is high, the separation effect is good and the like, solves the problem that the content of poisonous phenolic acid in ginkgo leaf extract exceeds the standard, and is an environment-friendly, economic and industrialization-suitable technique.

Description

technical field [0001] The invention belongs to the field of separation of Chinese herbal medicine components, and in particular relates to a separation technology of ginkgo biloba multi-components. Background technique [0002] Ginkgo biloba has high medicinal value. Ginkgo biloba and its medicinal effects have been recorded in the treatises of traditional Chinese medicine of all dynasties in my country, previous pharmacopoeias and many foreign studies. Studies have shown that the special chemical composition of Ginkgo biloba is an important factor for its wide range of pharmacological effects. So far, more than 160 compounds have been found in Ginkgo biloba, including: flavonoids, terpenoid lactones, polysaccharides, phenolic acids, polyprenols and other components. [0003] At present, the separation, in-depth research and utilization of natural active ingredients in Ginkgo biloba are mainly focused on flavonoids and terpenoid lactones, while the research and utiliza...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C33/02C07C29/76C07D311/30C07D311/40C07G99/00C08B37/00
CPCY02P20/54
Inventor 魏巧容郭震宇张河溪吴松刚黄建忠林金科郭耀荣
Owner 福建华尔康生物科技有限公司
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