Method of preparing a plurality of active substances from ginkgo leaves

Abstract

The invention discloses a method of preparing a plurality of activation materials from ginkgo leaves, and belongs to a manufacturing method of chemical substances or a manufacturing method of drugs. Strong polarity macroporous adsorption resin or polarity macroporous adsorption resin is adopted for separation, and is adopted to solve the problems that the amount of ginkgolic acid is high when the prior ginkgo extract is prepared, and solve the problems that a large amount of organic solvent is consumed when the prior ginkgo active substances are extracted and prepared, and accordingly the environment is polluted, or that a complex separation technology can not enlarge production, or that extraction efficiency is not high, and use ratio of raw materials is low and the like. The method of preparing the plurality of activation materials from the ginkgo leaves can simultaneously obtain a plurality of active substances such as ginkgo polysaccharide, ginkgol biloba extracts, ginkgol biloba general flavone, ginkgol biloba total lactones, gbilobalide A monosome, gbilobalide B monosome, gbilobalide C monosome and the like. The technical scheme is that the method comprises a raw material pretreatment step, an extraction step, a polysaccharide precipitation step, a macroporous resin separation step, a silica gel dry-column separation step, a reversed phase chromatography purification step, a crystallization step and the like, saves production cost, and improves work efficiency. The process of the method is simple and convenient to conduct. The method can be easily enlarged to the commercial process, and meanwhile greatly increase use ratio of raw materials.

Description

technical field [0001] The invention belongs to a manufacturing method of chemical substances or medicines, and in particular relates to a method for obtaining various active substances by extracting, separating and purifying ginkgo leaves as raw materials. Background technique [0002] Ginkgoaceae plants widely existed in Asia, Africa, Europe, South America and Australia 200 million years ago. Almost all over the earth's surface. Due to the great changes in the earth during the Tertiary and Quaternary periods, most of the Ginkgoaceae plants became extinct, and only one species of Ginkgo biloba survived, so it is called a "living fossil" plant. Ginkgo is a unique precious tree species in my country, especially in the south of the Yangtze River and Taiwan Province, accounting for more than 70% of the world's total ginkgo resources. With the excavation and development of the comprehensive value of medicinal and health care components in Ginkgo biloba, the systematic research...

AI Technical Summary

Problems solved by technology

[0006] The invention provides a method for preparing various active substances from ginkgo leaves, which is separated by strong polarity or polar macroporous adsorption resin, solves the problem of high ginkgolic acid in the existing preparation of ginkgo extract, and solves the problem of existing extraction and The preparation of ginkgo active substances consumes a large amount of organic solvents, causing environmental pollution; or it is difficult to scale up production by using more complicated separation techniques; or the extraction efficiency is not high, and the utilization rate of raw materials is low, etc.