12 results about "Bilobalides" patented technology

The invention discloses an application of bilobalide in preparation of a product for resisting salmonella typhimurium infection. The bilobalide takes salmonella typhimurium QseB protein as a target, has a good interference effect on an AI-2 quorum sensing signal of salmonella typhimurium and has good pharmacological activity, and particularly, the bilobalide has good pharmacological activity under the condition that the growth of salmonella typhimurium is not inhibited. The motility, biofilm formation and infection pathogenicity of salmonella typhimurium are obviously inhibited. The bilobalide does not influence the growth of salmonella typhimurium, directly acts on pathogenic factors, weakens the pathogenicity of bacteria from the source, does not generate selective pressure on salmonella typhimurium, and does not cause the generation of drug-resistant pathogenic bacteria. Therefore, the bilobalide has a good application prospect in the aspect of development of the salmonella typhimurium infection resisting medicine.

Provided herein are bilobalide derivative compounds, processes for their preparation, methods of use, and uses for the prevention or treatment of nervous system diseases and cancer.

The invention discloses a gene GbCYP701A6 for promoting synthesis of ginkgo terpene lactones and application of the gene GbCYP701A6. The nucleotide sequence of the gene GbCYP701A6 is shown as SEQ ID NO.1, and the amino acid sequence of protein expressed by the gene GbCYP701A6 is shown as SEQ ID NO.2. The invention further discloses a preparation method of the gene GbCYP701A6. According to the invention, a brand new gene GbCYP701A6 is cloned from gingko for the first time. The GbCYP701A6 gene is transferred into a ginkgo biloba body, the GbCYP701A6 gene is over-expressed, the contents of terpene lactones including ginkgolide A, ginkgolide B, ginkgolide C and bilobalide in ginkgo biloba are remarkably increased, and the contents of the ginkgolide A, ginkgolide B, ginkgolide C and bilobalide are remarkably reduced when the gene is silently expressed, so that the gene is a key gene for promoting synthesis of the ginkgo terpene lactones, and the GbCYP701A6 gene can be used for preparing the ginkgo biloba terpene lactones. Therefore, the regulation and control of the expression of the GbCYP701A6 has important application value in the aspects of improving the medicinal quality of the ginkgo leaves and the like.

This invention provides a composition of Ginkgo biloba extract and its preparation method. The composition consists of Ginkgo biloba extract (calculated as total flavonol glycosides) and dipyridamole in a dosage ratio of 10:4. The preparation method can significantly improve product yield, reduce production costs, and significantly increase the content of the main components of the produced Ginkgo biloba extract, namely total flavonol glycosides and terpene lactones. It can also reduce the content of poorly water-soluble ginkgolides and harmful impurities such as ginkgolic acid, control its microbial level, and improve the stability and safety of the Ginkgo biloba extract preparation product of this invention.

The invention provides a composition of ginkgo biloba extract and a preparation method thereof, the composition is composed of ginkgo biloba extract (based on total flavonol glycosides) and dipyridamole according to a dosage ratio of 10: 4, and the preparation method can significantly improve the product yield, reduce the production cost and improve the product quality. The ginkgo biloba extract prepared by the method has the advantages that the content of main components, namely total flavonol glycosides and terpene lactones, of the ginkgo biloba extract is obviously increased, the content of bilobalide with poor water solubility and the content of a harmful impurity, namely ginkgolic acid can be reduced, the microbial level of the ginkgo biloba extract is controlled, and the stability and safety of the ginkgo biloba extract preparation product are improved.

The invention relates to the technical field of medicines, in particular to application of bilobalide or stereoisomers thereof to preparation of medicines for preventing or treating obesity and obesity complications and preparation of anti-aging medicines or health-care products. The bilobalide disclosed by the invention can be used for effectively controlling the weight gain of mice, reducing the weight of epididymis fat of the mice, improving the holding power of the mice and increasing the muscle proportion of the mice in a high-fat feed-induced obesity model, and can be used for preparing medicines for preventing and treating diet-induced obesity, preparing anti-aging medicines or health-care products and the like.

The invention discloses application of bilobalide in inhibiting migration and invasion of ovarian cancer cells, and relates to the technical field of biological medicines. Application of bilobalide in preparation of drugs for inhibiting migration and invasion of ovarian cancer cells; the concentration of the bilobalide is 5 [mu] M to 40 [mu] M. In the application of the bilobalide, the bilobalide shows an obvious growth inhibition effect on ovarian cancer cells and has no obvious influence on the survival rate of normal ovarian cells; in a concentration range of 5-40 [mu] M, bilobalide shows dose dependence on activity inhibition of SKOV3 and ID-8 cells, but has small influence on IOSE80 cells, and shows a good safety window; compared with a traditional chemotherapeutic drug, the bilobalide as a natural compound has better biocompatibility, and is expected to be capable of remarkably relieving common toxic and side effects such as myelosuppression and liver and kidney function impairment in clinical application.

The invention discloses application of a DNA methylation inhibitor in regulation and control of synthesis of ginkgo terpene lactones in the technical field of plant cultivation, and the epigenetic regulation and control effect of 5-azacytidine on leaf growth and terpene lactones synthesis is researched by taking ginkgo as a material. 5-AzaC with different concentrations is set for treatment, and the dynamic change of leaf growth traits and terpene lactone accumulation is systematically analyzed. Researches show that treatment of 5-AzaC shows a significant dose effect: low concentration (50 [mu] mol / L) promotes increase of fresh weight and increase of terpene lactone content, and high concentration (greater than or equal to 150 [mu] mol / L) inhibits growth but significantly increases accumulation of bilobalide and ginkgolide C. Correlation analysis shows that biomass and terpene lactone content are in significant negative correlation, a growth and defense balance phenomenon is proved, the invention reveals that 5-AzaC affects synthesis and accumulation of different terpene lactones by regulating DNA methylation level difference, and a theoretical basis and a technical path are provided for accurate regulation of ginkgo medicinal components.

The invention provides a quality detection method and application of a ginkgo and American ginseng preparation, and relates to the technical field of pharmaceutical analysis. The quality detection method comprises the following steps: taking the gingko and American ginseng preparation, crushing, sieving, weighing, adding a solvent for extraction, filtering by a filter membrane, evaporating filtrate to dryness, adding methanol into residues for dissolution, and fixing the volume to obtain a test solution; the solvent is a mixed solution of n-butyl alcohol, methanol and acetic ether; the method comprises the following steps: weighing reference substances including bilobalide, bilobalide A, bilobalide B, bilobalide C, ginsenoside F11, ginsenoside Rb1, ginsenoside Re and ginsenoside Rg1, and mixing the reference substances with a solvent to obtain a reference substance solution; determining the reference substance solution and the test solution by using a liquid chromatograph to obtain a chromatogram; the quality detection method has the advantages of being simple, rapid, stable, reliable, high in precision, good in reproducibility, easy to master and the like, and the saponin, the lactone and other components can be detected at the same time.

The invention discloses a preparation method of ginkgo total lactone and bilobalide monomers contained in the ginkgo total lactone, which comprises the following steps: (1) dissolving a ginkgo leaf extract, extracting with a mixed solvent, taking a mixed solvent layer, and concentrating under reduced pressure until the mixed solvent layer is dry to obtain a ginkgo total lactone crude product; (2) adding dilute ethanol into the crude product of the ginkgo total lactone, heating, pulping, crystallizing, filtering and drying to obtain the ginkgo total lactone; (3) adding a high-concentration ethanol water solution into the ginkgo total lactone, heating, dissolving, crystallizing, filtering, concentrating mother liquor to be dry, repeating the step, and concentrating secondary mother liquor to be dry to obtain a bilobalide monomer crude product; and (4) dissolving the crude bilobalide product with dilute ethanol, adsorbing through macroporous adsorption resin, eluting, concentrating to be dry, and recrystallizing once to obtain the bilobalide monomer. The method has the advantages of few process steps, simplicity in operation, low cost and high product content and yield, and is suitable for industrial production.

The application provides a quality detection method and application of ginkgo ginseng preparation, and relates to the technical field of pharmaceutical analysis. The quality detection method comprises the following steps: crushing and sieving the ginkgo ginseng preparation, weighing, adding a solvent for extraction, filtering with a filter membrane, evaporating the filtrate, dissolving the residue with methanol, constant volume, and obtaining a test sample solution; the solvent is a mixed solution of n-butanol, methanol and ethyl acetate; a control sample of ginkgo lactone, ginkgo lactone A, ginkgo lactone B, ginkgo lactone C, ginsenoside F11, ginsenoside Rb1, ginsenoside Re and ginsenoside Rg1 is weighed, mixed with a solvent, and a control sample solution is obtained; a liquid chromatograph is used to determine the control sample solution and the test sample solution, and a chromatogram is obtained; the quality detection method has the advantages of simplicity, rapidness, stability, reliability, high precision, good reproducibility, easy operation and the like, and can simultaneously detect the above-mentioned saponins and lactones and the like.

The present invention belongs to the field of biomedicine technology and discloses a stem cell preparation for treating arthritis and a preparation method thereof. The stem cell preparation for treating arthritis of the present invention comprises umbilical cord mesenchymal stem cells pretreated with protocatechuic acid and bilobalide, and a cell protection solution. The umbilical cord mesenchymal stem cells pretreated with protocatechuic acid and bilobalide can effectively inhibit inflammatory responses. The stem cell preparation provided by the present invention can promote the expression of Collagen II and Aggrecan, proteins related to cartilage repair in mice, and is suitable for alleviating inflammation and repairing cartilage tissue in arthritis diseases, thereby achieving symptom relief for patients.