Method for removing and enriching ginkgolic acid from ginkgo biloba extract

A technology for ginkgolic acid and ginkgo leaves, which is applied in the field of removing and enriching gingkgolic acid, can solve the problems of high price, low purity of total ginkgo acid, and poor selectivity of gingkgolic acid, and achieve simple regeneration method, strong selectivity, and reduced cost Effect

Active Publication Date: 2014-12-10
SHANDONG ANALYSIS & TEST CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Mainly there are the following reasons: the template "ginkgolic acid" is expensive, considering the cost and income, it is unreasonable to use the template "ginkgolic acid" to prepare molecularly imprinted polymers on a large scale. If general substances are used instead of ginkgolic acid, it will The selectivity of ginkgolic acid molecularly imprinted polymers to ginkgolic acid is poor, and the purity of the total ginkgo acids obtained is very low. So far, there is no cheap substance to replace ginkgolic acid, and the obtained molecularly imprinted polymers have strong selectivity , can be used for targeted enrichment of ginkgolic acid

Method used

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  • Method for removing and enriching ginkgolic acid from ginkgo biloba extract
  • Method for removing and enriching ginkgolic acid from ginkgo biloba extract
  • Method for removing and enriching ginkgolic acid from ginkgo biloba extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] The flow chart of molecular imprinting technology removal and enrichment of ginkgolic acid is as follows: figure 1 As shown, the specific steps are as follows:

[0036] Preparation of ginkgolic acid pseudo-template molecularly imprinted polymers:

[0037] (1) material preparation

[0038] Each raw material component is formulated according to the following dosage ratio: pseudo template molecule 2-hydroxy-6-alkoxybenzoic acid (n=15) 0.99g, functional monomer 4-vinylpyridine 1.66g, crosslinking agent dimethyl Ethylene glycol acrylate 16.12g, initiator azobisisobutyronitrile 0.13g, pore forming agent chloroform 45.34g;

[0039] (2) Preparation of polymers with template molecules

[0040] Add the above prepared 2-hydroxy-6-alkoxybenzoic acid, 4-vinylpyridine, ethylene glycol dimethacrylate, azobisisobutyronitrile, and chloroform into the reaction kettle and mix evenly. Gas for 15 minutes, then filled with nitrogen and deoxygenated for 15 minutes, sealed and polymerized...

Embodiment 2

[0047] Preparation of ginkgolic acid pseudo-template molecularly imprinted polymers:

[0048] (1) material preparation

[0049] The components of this example are formulated according to the following dosage ratio: template molecule 2-hydroxy-6-alkoxybenzoic acid (n=0) 0.91g, functional monoacrylamide 1.83g, crosslinking agent N,N-dimethyl Base diacrylamide 24.00g, initiator azobisisobutyronitrile 0.15g, solvent chloroform 50.00g.

[0050] (2) Preparation of polymers with template molecules

[0051] Add 2-hydroxy-6-alkoxybenzoic acid, acrylamide, N,N-dimethylbisacrylamide, azobisisobutyronitrile and chloroform prepared in step (1) into the reaction kettle and mix well , ultrasonically degassed for 15 minutes, then filled with nitrogen and deoxygenated for 15 minutes, sealed and polymerized in a constant temperature water bath at 60°C for 24 hours to obtain a polymer with template molecules;

[0052] (3) Preparation of molecularly imprinted polymers that remove template mole...

Embodiment 3

[0058] Preparation of ginkgolic acid pseudo-template molecularly imprinted polymers:

[0059] (1) material preparation

[0060] The components of this embodiment are formulated according to the following dosage ratio: template molecule 2-hydroxyl-6-alkoxybenzoic acid (n=1) 0.85g, functional monomer methacrylic acid 1.66g, crosslinking agent divinylbenzene 21.10 g, 0.10 g of initiator (azobisisovaleronitrile), 47.00 g of solvent chloroform.

[0061] (2) Preparation of polymers with template molecules

[0062] Add 2-hydroxy-6-alkoxybenzoic acid, methacrylic acid, divinylbenzene, azobisisovaleronitrile, and chloroform prepared in step (1) into the reaction kettle and mix evenly, and ultrasonically degas for 15 minutes. Then fill with nitrogen and remove oxygen for 15 minutes, seal and polymerize in a constant temperature water bath at 60°C for 24 hours to obtain a polymer with a template molecule; then grind it through a 200-mesh sieve to obtain a molecularly imprinted polymeri...

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Abstract

The invention discloses a method for removing and enriching ginkgolic acid from ginkgo biloba extract. The method comprises the following steps: carrying out column filling by taking a molecularly imprinted polymer of ginkgolic acid as column chromatography packing; then filling the column with a ginkgo biloba extracting solution; leaching the column with a mixed solution of cyclohexane and ethyl alcohol in a volume ratio of 10 to 1; finally, eluting the chromatographic column with alcohol so as to obtain an effluent, namely ginkgolic acid, wherein the alcohol can be of ethyl alcohol, isopropanol, n-propyl alcohol or n-butyl alcohol. A molecular imprinting technique is applied to the removal of ginkgolic acid during ginkgo biloba processing for the first time, so that compared with the traditional ginkgolic acid removal method, according to the method disclosed by the invention, ginkgolic acid with high purity is prepared while ginkgolic acid is removed by virtue of the directional enrichment effect of the molecularly imprinted-solid phase extraction column, thereby providing the source of a raw material for clinical pharmacological research on ginkgolic acid.

Description

technical field [0001] The invention belongs to the technical field of traditional Chinese medicine preparation, in particular to a method for removing and enriching ginkgolic acid from ginkgo biloba extract. Background technique [0002] Ginkgo biloba is a rare and precious tree species unique to my country. It is known as the "living fossil of the plant kingdom". It originates in my country and is listed as a national second-class protected plant. my country's ginkgo leaf resources account for 70% of the world's total, ranking first in the world. There are many studies and reports on the active ingredients of Ginkgo biloba at home and abroad. Ginkgo biloba preparations are currently one of the most widely used natural medicines in the world for adjuvant treatment of cardiovascular and cerebrovascular diseases. They have unique curative effects and are widely used in medicines, dietary supplements, etc. . [0003] Ginkgolic acid (Ginkgolic Acids, referred to as GAs) is an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/16C08J9/28C08J9/26C08F222/14C08F222/38C08F212/36C08F220/56C08F220/06C08F226/06
Inventor 王晓纪文华赵恒强耿岩玲马秀丽谢宏凯陈凌霄
Owner SHANDONG ANALYSIS & TEST CENT
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