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Use of ginkgoic acid in preparation of biological pesticide for killing Oncomelania snail and preventing schistosomiasis

A biopesticide and ginkgolic acid technology, applied in the direction of biocides, chemicals for biological control, biocides, etc., can solve the problems of high price, environmental pollution, hazards, etc., and achieve the effect of preventing schistosomiasis

Inactive Publication Date: 2006-07-26
FUDAN UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Sodium pentachlorophenate has been used in many countries. It has a good molluscicide effect, but it has broad-spectrum toxicity to non-target organisms, especially fish and shrimp farming, and causes serious environmental pollution. It has long been listed as a banned product by the international community
Niclosamide (Niclosamidum) has a good molluscicide effect and is recognized as a molluscicide in the world, but it is expensive and its use in large areas in developing countries is restricted. It will also cause harm to aquatic organisms such as fish when applied in high concentrations. and can lead to increased resistance of target organisms

Method used

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  • Use of ginkgoic acid in preparation of biological pesticide for killing Oncomelania snail and preventing schistosomiasis
  • Use of ginkgoic acid in preparation of biological pesticide for killing Oncomelania snail and preventing schistosomiasis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1 The effect of ginkgolic acid (C13:0) components on killing snails

[0037] 1. Extraction of ginkgolic acid:

[0038] Add 100g of dried and crushed Ginkgo testa, add petroleum ether (boiling range 60-90°C) in a ratio of 1:5 for ultrasonic extraction for 1 hour, filter, repeat the extraction of the filter residue twice, combine the extractions, and evaporate the petroleum ether to obtain the exocarp. Skin crude extract extract.

[0039] The extract was dissolved in a small amount of petroleum ether and loaded onto a silica gel column (120 g of silica gel, the silica gel column was a Ф32×400 mm glass column, packed by wet method), and the mixture was mixed with petroleum ether: ether: formic acid = 89: 11: 1 ( V / V / V) elution, with thin-layer chromatography appearing fluorescence at 254nm as detection, collect the ginkgolic acid components, concentrated to dryness; repeat the column once, the obtained ginkgolic acid components were washed to neutral, concentrated...

Embodiment 2

[0050] Example 2 The effect of ginkgolic acid (C15:1) components on killing snails

[0051] 1. Extraction of ginkgolic acid (C15:1):

[0052] Dissolve the above testa crude extract extract with a small amount of petroleum ether and apply it on a silica gel column (120 g of silica gel, the silica gel column is a glass column of Ф32 × 400 mm, packed by wet method), and use petroleum ether: ether: formic acid = 89 : 11:1 (V / V / V) elution, detected by the appearance of fluorescence at 254nm in thin-layer chromatography, collecting the fraction with the largest spot among them, and concentrating to dryness to obtain the ginkgolic acid (C15:1) fraction.

[0053] 2. Analysis and purity determination (reversed-phase HPLC method):

[0054] The 15cm C18RP-HPLC column is connected with the 20cm silver-loaded cation HPLC exchange column and is used, with methanol: water=93: 7 (acidified with 0.1% formic acid) as mobile phase, and the ultraviolet detection wavelength is 311nm, to the above...

Embodiment 3

[0062] Example 3 Killing effect of Ginkgo biloba exotesta crude extract extract on Oncomelania snails

[0063] 1. Preparation of ginkgo testa crude extract:

[0064] Add 100g of dried and crushed Ginkgo testa, add petroleum ether (boiling range 60-90°C) in a ratio of 1:5 for ultrasonic extraction for 1 hour, filter, repeat the extraction of the filter residue twice, combine the extractions, and evaporate the petroleum ether to obtain the exocarp. Skin crude extract extract.

[0065] 2. Analysis of the content of ginkgolic acid in the crude extract of Ginkgo biloba testa (reversed-phase HPLC method):

[0066] Measured by the above-mentioned RP-HPLC method, the content of total ginkgolic acid in the crude extract of ginkgo testa is 5.46%.

[0067] 3. The effect of soaking the extract of ginkgo testa crude extract to kill oncomelania:

[0068] Dissolve the crude extract of ginkgo biloba testa with a small amount of absolute ethanol, and then dilute with boiled and cooled tap w...

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Abstract

The invention relates to the use of ginkgoic acid in preparation of biological pesticide for killing Oncomelania snail and preventing schistosomiasis, wherein experiment result shows that, the ginkgolic acid can effectively inhibit key enzyme and de-coupling reaction to mitochondria oxidation phosphorylation in mollusk energy supply biochemical metabolism, effectively inhibit tyrosinase during insect exuviation procedure and prostaglandin synthetase during breeding process, thus can eradicate unwanted mollusk harming agronomic crops.

Description

technical field [0001] The invention belongs to the field of biological pesticides, and relates to the new application of ginkgolic acids in the preparation of biological pesticides, in particular to the use of ginkgolic acids for killing oncomelanias and preventing schistosomiasis and its application as a novel biological pesticide. Background technique [0002] The snail (Oncomelania hupensis, O. hupensis) belongs to the mollusk, and is the only intermediate host for spreading the widespread schistosomiasis in my country. [0003] The miracidia of schistosomiasis can develop into schistosomiasis after invading the snail, which multiplies tens of thousands of times in the snail, and continuously produces cercariae in the water or on the wet soil surface, so as to infect humans and other animals. The spread and spread of the disease. Elimination of oncomelania can cut off the transmission route of schistosomiasis. Therefore, killing snails is an internationally recognized ...

Claims

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Application Information

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IPC IPC(8): A01N37/10A01P9/00
Inventor 潘家祜谢德隆高崎毛佐华俞培忠潘小海孙锴
Owner FUDAN UNIV
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